Exploring the fascinating development of cannabinoid-producing trichomes - Zamir Punja, PhD
ฝัง
- เผยแพร่เมื่อ 4 ก.ย. 2024
- Tags: cultivation, potency, cannabinoids, terpene, resin, cannabis growing, yield, harvest
Zamir Punja presents “Exploring the fascinating development of cannabinoid-producing trichomes in cannabis flowers” at CannMed 2022.
Glandular trichomes produced on bracts of female inflorescences of THC-containing plants are known to produce cannabinoids. What are the details of how these structures develop ? The fascinating process of capitate stalked trichome development was studied using light and scanning electron microscopy (SEM). Two strains- Moby Dick and Space Queen - were compared at 3 weeks and 6 weeks of the flowering period. Four morphologically distinct trichome types will be shown: nonglandular hairs (stacoliths) and three glandular (secretory) forms -pedicillate bulbous (15-30 um in diameter), sessile peltate (30-45 um in diameter) and capitate (40-85 um in diameter) that were stalked or sessile. The stalked capitate trichomes are formed in greater numbers on lower compared to upper bract surfaces, and at higher frequency on Space Queen compared to Moby Dick as maturity of the inflorescence progressed. Trichome glands increased in volume with maturity and resin was secreted through cracks or tears in the surrounding cuticle, resulting in collapse of the trichome gland. Dried buds of four cannabis strains showed varying trichome densities, stages of development, trichome stalk length, and degree of gland dehiscence. These findings may be extrapolated to develop a method to estimate trichome gland maturity to optimize cannabinoid levels at harvest.
I have been taking pictures of trichomes with microscopes for like 15 years. But Wow these pictures are phenomenal amazing stuff doc.
@@Bombarded1n this is about real weed not no detla 8 shit
@@_cspr delta 8 is hemp, not cannabis 😂
@@borbleborb4586 im well aware lol
@@_cspr just sayin that’s a weird thing to pull outta nowhere lmao
@@borbleborb4586 superstrain is a delta 8 brand, why i said it to the guy talkin about superstrains for whatever reason
Who is going to be the hero who captures a microscopic time-lapse of trichomes growing/maturing. He'll get the nobel prize
Great stuff. I just love it when the former hippie plant becomes scientifically explored. Keep em coming!
Former hippie plant wow so ignorant
The first “acid test” grateful dead shows were the first attempt at a “Meta-verse” where they provide you with their version of reality, they’re bohemian grove members
When a former hippie plant what other hippie plants you talking about?
@@williambarringer6513 Please elaborate
but GAWD gave us this plant and the evil goobermint stole it from us
is this what happens when a scientist gets a really really really good bag?
😂😂😂😂😂😂
The most intelligent and beneficial plant on the earth!
Banisteriopsis caapi says 'hello'
Some of those magnified images belong on a wall as posters, beautiful
I actually have a 12 month calendar made up. Each month is a different trichome photo ! Pretty awesome as you wait for the next months image to show up !
@@zamirpunja1978 You should sell them!
harvested at 7 weeks, dried for 5 days then sold. thats why you grow your own 💀
Perlite n sprays.
Cureing for months is the best.
Hot Crust Buns! I love the science and research going on, and the fact that there is some much left to discover.
I watched this video in the morning drinking my coffee. Had a good laugh when he used hot crust buns to describe two bulbs stuck together 😂😂
Beautifully detailed trichome presentation 💚🙏
Yes I was *fascinated* computers will bring us the best cannabis ever. Thanks Mr. Z. Punja
The visuals really helped me grasp the concept better. Incredible research and breakdown here, thank you for this!
This was very informative, thank you for posting.
This is one part of the future that I am loving!
Tremendous and interesting exploration, as a grower I have always been impressed by the cannabis plant and how intelligent its whole structure is, of course all plants have amazing systems, but cannabis has that special susbstance, I have taken very good pictures of the trichomes when the plants are in the sun and these trichomes in the telescope look great, from now on I will follow the channel because I got very good information from this video.
You mean scanning electron microscope 😀
@@websurfer5772 (slow clap) thank you!!! this isnt the same as using a jewelers loupe to check if harvest ready or using a 1000x to check for other things or just see progress this is an electron microscope something the vast majority of us will never have access to so value that aspect also its an amazing machine
The energy going into filling the globular trichomes with enzymes, cannabinoids and THC.. all part of the specie's propagation and survival strategy.
Once in a while the work seems quite noticeable, IMO that's one rare sample right here.
Holy trichomes Batman. Wow wow wow
Amazing video! I really appreciate you breaking down the structures so thoroughly. You mentioned the enzymes in the trichomes that manufacture terpenes and cannabinoids, and I was wondering you knew any papers or research that went into detail about that? How plants manufacture chemicals like these has been fascinating to me for awhile, and I hope to do research on it in the near future. Thank you again!
Have you heard of google? or incapable of reading and doing basic research...
@@jakelooter5139 why the need to be so condescending? I have looked it up before, and since leaving this comment I have researched it quite a lot. I was just asking for help from professionals, whose whole channel is dedicated to sharing information on cannabis.
@@adultpersonman4612Jakelooter needs a big fat doobie to calm them down.
My main english is not even english, im a biology student, you speak so easily and so precise, and the images are fabulous!! Thank you so much!!
I wonder what they did with "The study material" after because it looks amazing.
what a wonderful, thourough and educative video!! It wouldve been nice to see how different the trichomes differ im a Sativa/Imdica..but seriously as a seasoned cannabis grower this just blew my mind. I learned so much more about trichomes!!! and cannabis itself.. best video ever! subbed!!
"Educative" 🤔
@@Armistead_MacSkye 🤡
Can you do a video like this for Psilocybe Cubensis? Another completely beautiful organism with such powerful molecules ❤️
yes to shrooms 💯
nature has the BEST molecules, man
Can't wait to see more development on this.
the involvement of endocannabinoids in bone growth remodeling has become clinically relevant. Thanking all breeders cultivators lovers of the master plant. She breeds passion and homeostasis cb2 receptor inhibits sars2. Cannabis is a medicinal treasure trove peace and love
5 days to dry? You've got to be joking, or misinformed.
Usually 10-14 days and then a curing period after that.
5 days and you'll end up with flavourless dusty crispy grass clippings.
thats not true
Experience is your best teacher! Go for it!
Nice work. You’ve answered several questions that have been swirling around my head. Great images too.
I can't help but wonder what evolutionary pressure or benefit caused the plant to develop the trichomes?
Afaik the trichomes are a natural shield/reflector against radiation.
The plant comes originally from the Nepal/Hindukush area. Means, she had to fight the radiation of the sun for propably millions of years. In my opinion it is some kind of a natural evolutionary developed shield against UV and other to biological lifeforms hazardous radiations (be it from the ultra short or ultra long spectrum of light). Well, that is at least what i got from reading deep into it back in the days. I was already growing in the 90s, and i remember that we already had studies from scientists floating around at that time. Cant remember specific names, but i definitely know that science researched that already 20-30 years ago. If i remember an article or scientific paper about it, then ofc i will add it here as comment.
@@insideaero7660 You know, that make perfect sense, there would have to be a shading effect from the quantity. Thanks!
God
seed protection OBVIOUSLY they are extremely coveted by animals for food.
Thank you very much for the science and for sharing information.
fascinating lecture. love this incredible plant
Excellent talk! I am a molecular virologist and geneticist turning his eyes towards cannabis research...
Can you please tell me, has any scientist ever actually seen a virus? I'm dying to know.
Also, has anyone ever seen a double-helix, twisted, DNA molecule?
Thank you very much for the pictures and information.
I Would I like to see as a agronomist and horticulturist that some of hybrids be used in the landscape industry. Of course without the THC . But still provide the colors and fragrance. Unlike flowers and other flowering plants, cannabis holds its fragrance for almost the life of the plant . Something to think about
it quickly and aggressively chokes out all other species though which makes gardening somewhat difficult.
Thc is the best part!
Cbd flowers dude
no it doesnt. it doesnt even smell until near the end, when the terpene profile changes daily and weekly. Why no THC? As a "horticulturist" LOL you should know, that it is the SAME SPECIES Cannabis sativa, ie NO HYBRIDS genetically. all of you fake scientists on here trying to look intelligent because you are pseudointellectuals w barely above average IQs...and know it. No one cares what you want to see either FYI...
@@nickhowatson4745 no it doesnt most weeds will overtake it in a fertile environment...
Great video. Very educational..love tge the electron microscope images..thx fir sharing
You definitely need to dry for more than 5 days, and it's 100% mids if you're packaging it up before a minimum of 3 weeks off the plant.
Yea I heard 5 days and was like hell naw
Lol ok dude
not necessarily...how is it mids if it is still 30 something percent THC and is well cured?
Amazing. Thank you so much for the information and the images
(Artists conception of a glowing 4 meter tall trichome) “It would be wonderful to see these on our streets...”. No shit. I love this guy!!
5 days of drying and they are ready for packaging? Say what?
Would have been interesting to compare outdoor, vs indoor trichomes!
I agree. I bet with the outdoor you would see many different dead insects that got stuck inside these trichomes
it could be awesome
Hi Carl - yes I have not looked at outdoor flowers to see the trichomes. They could look quite different, being exposed to different environmental stresses.
@@papacos4745 I get fruit flies on my buds which are indoor, outdoor UK not many pests but snails nibble a bt but thats ok, snails gotta eat too!
@@papacos4745 Yes like looking into amber
WOW!!! Spectacular!
Extremely fascinating.
you have such a clear way of communicating this information to us, this is why i love scientists their minds work with such conscientiousness
Excellent!
Why don't they shake the resin off? I know it's sometimes laborious but it's gorgeous! This plant is so soooo helpful for humanity! It's seriously the most beautiful thing in the world!
Actually they do like that once is dried .
Loves me Florence,Flo was an original strain 35 years ago,stop inflammation use your tricolmes wisely and often, Peace
Very well done. Thank you. Look forward to more info from you. 😊
I would think there are more glands on the bottom because gravity works for free.
The really long trichome stalks are prob just stretching up out of the forest for light? Fascinating slides. I didn't know there were tricomes that never develope heads. Thought I was being too rough in handling.
Masterpiece, I loved your research
One of its kind
Absolutely gorgeous. Shows all the beautiful structures removed and destroyed in legal weed. Would be nice if they ever matched the black market in price and quality, but not holding my breath, no pun intended. Thank you for such amazing views of this fascinating plant.
😊 ❤ God is amazing, look at his creation.
There is a story from the Dogon tribe that says cannabis was a gift to the earth from beings that are from the Canis star system. And looking at this beautiful SEM pictures of the Trichomes it's easy to believe! .. it's worth remembering that that Tribe were able to describe star systems and constellations a looooong time before humans knew about them. Makes you wonder where they got that info. 🤔
Thoth the Atlantean, maybe?
Wow! Thanks! Amazing info. More please!
Outstanding presentation, excellent and interesting.
Wow, thank you for summing it up so nice.
Superb imagery
Very intersting. Great images and information
Re: trichome density on bottom. Maybe because insects tend to prefer the undersides of leaves. Just a guess. 22:02
Exceptional
Very cool to see at that magnification.
Imagine with colours. Microscopes can be cheap and i never had regrets buying mine, except for the mechanical support with no easy focus...
@@Egzoset everything at that magnification level is just amazing the world of the very small is just out this world it’s alive I’m interested in nano technology and life at this scale. We need to focus on this because it can really help us but it’s the tools 🛠️ to play with our nano world we also have to be very careful not to disturb it as it can cause massive problems like with gain of function research.🔬
very informative, super awesome! thank you for this
Makes me want to go light up, but a nap seems more appropriate. I'll push pause, do the nap thing, wake up, light up and finish enjoying this flick.
no one cares
I was already pretty familiar with the structure with glandular trichome head and the capitate stalk. These images are absolutely amazing though. It really gives me an amazing real life window into what these structures look like up super close. Also shows me the reason why that no matter how well you isolate the heads, the resulting hash will never quite melt down to no solid residue. Even with perfectly isolated heads, you still have those stipe cells and secretory cells on the underside that are not going to be melty. Even if you find a crazy mutation where those cells are super small, I'm guessing they'll still take up at least 10% of structure of the head. Which is why you can never get 100% melty product unless you blast it with butane or some other hydrocarbon, or press rosin out of it, to sort out the non-melty bits.
thaank you sir
absolutely wonderful work
Thank you this was Great!
Got to love how it has taken modern science to tell us what near eastern farmers have known for centuries
Very interesting science behind cannabis
Im here from the Madame Cannoli live stream !!
Oh man can you imagine these growing in ours streets!! I would be a very happy dude.
Cannabis trichomes are so cool.
This is Brilliant. Spliff time
Holy wow is this the best thing I've seen in years. I love marijuana and I have a degree in plant science from Penn State! I love this stuff, thanks so much!!
super cool! This makes me wonder how far we are away from some visual sensor that can tell us the moment at which the plant is fully developed and ready to harvest. That is the hardest part about being a home grower. Im never really sure if I am harvesting at the right time. Thank you for the presentation
What day of flowering are you harvesting?
Three basic stages of development based on visual cues: clear glandular heads means early in development, resulting in higher precursor compounds, milky are typically desired developmental stages for harvest in recreational application and denote a wider range of cannabinoid availability, and finally amber colored glandular heads will denote partial degradation of primary cannabinoids into things like CBN, which impart heavier affects commonly associated with extreme-case medicinal uses, such as alleviating cancer patient suffering or combatting insomnia. On second thought, a mix of amber and milky glandular heads are probably most ideal for extreme case medicinal use, as CBG is recommended for aiding symptoms associated with glaucoma, thca and D9thc are found to be anti-fungal/antibacterial and CBD is, in conjunction with THC compounds, considered beneficial for their anti-inflammatory and cancer-fighting potential.
This is a generalization and, by far, not the entirety of the subject knowledge/application potential, but I wanted to be brief for simplicity's sake.
The "hairs" or more accurately, stigmas, will turn orange as the virility of the female components degrade and aren't necessarily correlated with trichome development, in that they won't tell you about cannabinoid development as accurately as glandular head coloration will (to the best of my knowledge).
Joshua, experiment by clipping off a couple buds at timed intervals, carefully drying/curing the buds, keeping track of what each buds trichomes looked like when you clipped them. You can then compare clear, milky, and amber.
pretty well done.... full of knowledge to get the right education.......thank you very much ......
Excellent video!
This was super neat and informative.
I've always grown outside the last near 50 years and always used Female rather than going through the process of male & female My biggest problum being thick Buds ! Due the state I'm in around march humidity shoots up leading to mold ! I use the Trichome to decide when the buds are ready from a clear or look to the foggy look they get towards the end ! That way you can pick maximum THC production along with all the other traces / cannabinoids My next import requirement for me is the Taste and smell / terpenes ,The electron magnifying Puts my $100 one to shame But the same process ! Always the happy receiver of gifts if you feel the need to send your electron to Australia , great video thank you
microscope begging on youtube? literally one of the dumbest comments I have EVER seen on the internet period.
excellent presentation
Beautyfull.....enlightening......new.....deserving of a big screen view,.......also...how about a video of it growing?
Thank you for making this video. Such beautiful photos.
Beautiful images! Most non-users don't get it I'm sure.
Makes sense to me that there'd be more trichomes under than on top. Cannabanoids are partly pest replenents, as I understood it. And most pests will move from lower on the plant to higher.
Thank you for the presentation and sharing your knowledge
I'm curious about how a southern magnetic field would effect trichrome development. I like this lecture. Thanks for the work.
Hi Ben. great thought ! Not sure how magnetic fields may affect trichome development. Have to think more about that.
Ahahah you need to start put more tobacco on what are you smocking 😅😂😂
probably NOT AT ALL since that isnt what causes trichome development. back to the basics for you...
Very cool! Answered a lot of questions I’ve had!
Tetrahydrocannabinol is only one of the 113 total cannabinoids on the plant. Trichome glands are our friends.
Great presentation. Would love to see you dissect all three covid vaccines and use your microscope to look at all the elements in the vaccines! Your commentary would be dynamic, informing, and appreciated by the world, and probably used by all the professionals that are respected by the truth movement!
Yep this would be verry interesting
Buto way off topic, many questions about thricomes are still to be analysed by such a close cool
they already knew what was in it BEFORE rollout I know I did.,..
I stopped taking clones of a Cali gold strain that grew amazing, looked good, but it didn't have any smell. I grew 4 or 5 clones. Then bloomed the mother. I sifted most of the bud for kief and it made the craziest kief I e ever seen. It all melted. My theory was the gland heads perfectly fell off. I regretted blooming the mother.
they always fall off,,,
@@jakelooter5139 well then you must be a great hash maker. if they all fell off everyone would have super pure rosin and hash
Seeing those pics makes me giggle with joy!
what an amazing video. thanks for the presentation!
Awesome talk! Great images!
Thank you ❤️
Look so similar to mushroom formations. Tiny explosions of potential.
Not really...I am a widely known mycologist NOT a random idiot posting
The difference in trichomes on the underside must be to reduce any compromise and/or negation of photosynthesis, kind of in the same way we put black plastic balls in bodies of water to negate evaporation from sunlight.
Outstanding work, sir.
thankyou
Thank you, Doctor! I love this stuff!
Thank you
Very good, thank you for the education. I think it would be interesting to find out what differences there are between the aged triclome heads and fresh... the image of the dried head and separate stalk seamed to show tubes in the stalk and compartments in the base of the head. I am now fascinated to find out lol. Keep up the great work.
Very good presentation.
🙏
Thankyou Zamir Punja for this Excellent Presentation,Loved it :)
At 7 weeks these plants have the potential to develop further