The best brand of aquatic plant tissue culture is Tropica if you still want to go that route of starting your culture from existing TC. If you want to disinfect aquatic plants, the procedure that works best for me is an alum dip followed by bleach. Dipping aquatic plants in 2 tablespoon per gallon of alum or aluminum sulfate for 30 minutes (sorry, I will need to dig my notes for the more scientifically rigorous procedure in SI units) will kill invertebrate hitchikers. A solution of common household bleach in (3-5% strength) in 1 part bleach to 19 parts water can be used as a disinfectant dip. Soak sensitive and stem plants for no more than 20 seconds. Plants like Bucephalandra and Anubias can soak for up to 1 minute. It's best to use a sterile solution of sodium thiosulfate as a dip after the bleach solution to immediately stop bleach activity.
Aqua Design Amano mades some of the high quality tissue culture plants. Surprisingly they retail for the same price as these contaminated Top Fin TC’s.
Most aquatic plants sold, in the wild just live on the banks of rivers, so they have the ability to stay alive and grow while submerged during flood season, but aquatic plant farms grow them in humidity saturated greenhouses, not submerged. So I guess it's not essential to grow them sumberged in tissu culture either.
The main reason in the aquarium hobby for tissue culture plants, and why they're popular, is generally you end up with snails on non-TC plants. So, people know they won't get snails if they buy TC plants. Some people, falsely, believe that snails will eat their plants; they only eat decaying matter and are actually very good for the tank. Other people just don't like the look of snails.
Petsmart has been selling TC plants for probably 15 years now. Since there's no humidity shock issue when dropping them into an aquarium, the survival rate is very high, even for total novices, and they tend to actually have a longer "shelf life" in the store since most non-TC aquarium plants are grown out of the water in their emergent forms, so they usually look like hot garbage a week after arrival in the shop since they will dump their old leaves to grow new submerged ones.
You are definitely not a newb boob like me lol 😂I thought I knew what’s up, damn make me feel stoopid, this is so cool. Jelly of your setup, been getting into fish biology lately but never dedicated myself to plants like you, might just get into this.
Just potentially helpful heads up about growing aquatic plants, is that most of them actually don't like being grown immersed (too little CO2), and will much more happily grow emersed like any other typical plant. As a matter of fact nearly all aquarium plants are grown emersed, and they melt and die back as they transition to immersed. P.S. I also wouldn't sweat the pH either, the common plants are all really robust and can handle a wide range of pHs.
I think it's a stretch to say they don't like growing submerged. Of course many will grow faster emersed due to higher CO2 levels and more light, but that doesn't mean they don't like growing submerged. They are adapted to grow underwater so they can survive flooding in their natural habitats and so they can take advantage of a niche that most other plants can't.
@@pendlera2959 It's not a stretch at all. Yes, their niche is the marginal zone with their roots in water logged soil, and the capability of surviving immersed during flooding. But when you find them in nature, they are not growing underwater. They grow right up to the water's edge, maybe out to a few inches of water depth (but with foliage emersed). This is how you typically find plants like Anubias, Buce, java fern, Eleocharis, Bacopa, etc... immersed is definitely not their preferred location, while emersed is very strongly preferred. Are there true aquatics as well? Absolutely! Vallisneria, Potamogeton, etc... Which if you then look at Tropica's and Top Fin's tissue culture lines, they don't typically do immersed plants.
@@pendlera2959 the thing with aquatic plants they morph drastically when grown underwater vs emersed. so best to do it semi-aquatic not totally submerged tbh. faster growth rate, easier to adapt underwater.
100% noob question, if we had a plant that we wanted to get tc of(basically growing and ready to go) how much is that, and is there economies of scale for it?
You should have washed the plants with a bleach solution before proceeding It’s common to clean / sterilise plants In a mild/moderate solution of bleach Before adding them to aquariums or propagating them
I can’t remember who but I think on Garden Talk with Mr.Growit a lady was talking about using bleach solution because alcohol can actually help some unwanted things (a type of virus maybe?) grow
This might explain the notoriously poor performance of the top fin tissue cultures. I thought I was going to be the exception to the rule with some of their bucephalandra but unfortunately it did not do well for me in the aquarium environment
It’s interesting that the plants were noticeably contaminated only after they were subcultured. Is this to say that topfins sterilization technique is subpar and they are selling contaminated plants? Yet there is always a percentage of contamination
they're beneficial detritivores, but aren't something you want there if you didn't plan on it. also they harbor bacteria, and some fungi parasitize marine amphipods. and as someone with aquatic isopods and invertebrate friends, I cannot abide!
Ayyyoooo daaaaaamn. I was excited to buy a shirt from the website but its $28 for shipping on a single shirt bringing it to $60! Is there a discount code on shipping??? (PS Make a hoodie please)
It looks like it’s more of a pain in the ass than it’s worth I’ve bought a few tissue culture plants but they’re stupidly expensive compared to non tissue culture plants and I’m not payin’ that again
Its pronounced (roughly) Beu-sef-uh-laund-rah I'm not judging, because I have no doubt I've mispronounced my share of scientific names over the years. Just trying to be helpful on account of all the aquarium keepers who are probably having fits now. 😂 Also, Tropica generally has very clean TC cups; you can store them for weeks on a lighted shelf while preparing a new tank. If I planned to get TC cups for starting my own multiplication cultures, that's where I would source from. Top Fin stuff is cheap and often visibly contaminated, though as long as it hasn't completely overtaken the plants, that's not really a huge issue if you are dropping it directly into an aquarium (no special acclimation required; you open the bag, rinse under a tap to remove agar, and stick it in the tank). I buy it sometimes for my aquariums because it's readily available, but definitely wouldn't trust it to be sterile for cultures.
That's super common for readers, like that guy who read all the works of "plah-to". Everyone knows it's pronounced "play-to" (but most haven't read Plato's works). Super informative video as always, thank you!
And then you banned me from Discord, I thought that this was a place for tissue culture plants? Or are you in fact working for big tissue culture now? I'm just going to cry in a corner now...
@GreenCanvasInteriorscape because I posted photos of "abducted" or "poached" plants :( But most of my plants that come from Thailand have all been propagated by my father who lives in Thailand and have been brought through TSA (airport security). I understand that some people have problems with plants taken from other countries, but these plants are not protected in any form and you are allowed to bring them with you in sealed containers. I also own a few plants that have been given to me by friends that just took a cutting in the hope I would be able to propagate it...
@GreenCanvasInteriorscape sadly I can't share pictures on TH-cam, but I have 39 different species of plant in my windowsills and a big grow light to give them 18 hours of light.
Have you ever experimentaled with colloidal silver since it is antifungal antaiviral antimoldial and auntie bacterial. And it is great for human use as we all nose.
I don't know if I'll ever do these things, but I watch them with such a desire to get my hands dirty.
When will the pet smart meal video be? I'm expecting the video soon, Glorb.
Next time Glorb needs to feed
The best brand of aquatic plant tissue culture is Tropica if you still want to go that route of starting your culture from existing TC. If you want to disinfect aquatic plants, the procedure that works best for me is an alum dip followed by bleach. Dipping aquatic plants in 2 tablespoon per gallon of alum or aluminum sulfate for 30 minutes (sorry, I will need to dig my notes for the more scientifically rigorous procedure in SI units) will kill invertebrate hitchikers. A solution of common household bleach in (3-5% strength) in 1 part bleach to 19 parts water can be used as a disinfectant dip. Soak sensitive and stem plants for no more than 20 seconds. Plants like Bucephalandra and Anubias can soak for up to 1 minute. It's best to use a sterile solution of sodium thiosulfate as a dip after the bleach solution to immediately stop bleach activity.
That "fruit fly" in your Bucephalandra looks more like a fungus gnat, it definitely wasn't a fruit fly.
Bucephaladras will never be the same after this video
That “roll the clip” got me for some reason 😂 (I’m puffing a doob)
Happy to see that you’re working with bucephelandra! Such a rewarding plant to propagate. And by rewarding I mean profitable 😛
amazing the audio translate, thnaks so much i loved thanks from brazil!
That was an absolute classic intro!!! Lol
Aqua Design Amano mades some of the high quality tissue culture plants. Surprisingly they retail for the same price as these contaminated Top Fin TC’s.
Loved the intro
Most aquatic plants sold, in the wild just live on the banks of rivers, so they have the ability to stay alive and grow while submerged during flood season, but aquatic plant farms grow them in humidity saturated greenhouses, not submerged. So I guess it's not essential to grow them sumberged in tissu culture either.
The fact pet smart actually sell tissue culture plants is a wow factor...
The main reason in the aquarium hobby for tissue culture plants, and why they're popular, is generally you end up with snails on non-TC plants. So, people know they won't get snails if they buy TC plants. Some people, falsely, believe that snails will eat their plants; they only eat decaying matter and are actually very good for the tank. Other people just don't like the look of snails.
Petsmart has been selling TC plants for probably 15 years now. Since there's no humidity shock issue when dropping them into an aquarium, the survival rate is very high, even for total novices, and they tend to actually have a longer "shelf life" in the store since most non-TC aquarium plants are grown out of the water in their emergent forms, so they usually look like hot garbage a week after arrival in the shop since they will dump their old leaves to grow new submerged ones.
@@danarennick7003 Depends on the species. Some snails will eat your plants.
You are definitely not a newb boob like me lol 😂I thought I knew what’s up, damn make me feel stoopid, this is so cool. Jelly of your setup, been getting into fish biology lately but never dedicated myself to plants like you, might just get into this.
Just potentially helpful heads up about growing aquatic plants, is that most of them actually don't like being grown immersed (too little CO2), and will much more happily grow emersed like any other typical plant. As a matter of fact nearly all aquarium plants are grown emersed, and they melt and die back as they transition to immersed.
P.S. I also wouldn't sweat the pH either, the common plants are all really robust and can handle a wide range of pHs.
I think it's a stretch to say they don't like growing submerged. Of course many will grow faster emersed due to higher CO2 levels and more light, but that doesn't mean they don't like growing submerged. They are adapted to grow underwater so they can survive flooding in their natural habitats and so they can take advantage of a niche that most other plants can't.
@@pendlera2959 It's not a stretch at all. Yes, their niche is the marginal zone with their roots in water logged soil, and the capability of surviving immersed during flooding. But when you find them in nature, they are not growing underwater. They grow right up to the water's edge, maybe out to a few inches of water depth (but with foliage emersed). This is how you typically find plants like Anubias, Buce, java fern, Eleocharis, Bacopa, etc... immersed is definitely not their preferred location, while emersed is very strongly preferred.
Are there true aquatics as well? Absolutely! Vallisneria, Potamogeton, etc... Which if you then look at Tropica's and Top Fin's tissue culture lines, they don't typically do immersed plants.
@@pendlera2959 the thing with aquatic plants they morph drastically when grown underwater vs emersed. so best to do it semi-aquatic not totally submerged tbh. faster growth rate, easier to adapt underwater.
Next video for the Biocoupler say legal "hemp" instead of cannabis 😉 also.... uga uga!
good experiment
I was hooked by the first idea
Your pronunciation of Bucephalandra would be approved by most Latin/botanical Latin speakers. Third comment, I am going to stop now.
Thanks for the "do not google" warning!
Lots of people grow "tomatoes" in hydroponic culture, I believe.
Interesting findings! Haha! Yeah YT punishes for the c-word.. :) Thanks for sharing!
The joke hook must now be on every video!! Make it your thing!!
Interested in the weird protocol you've said in the video 4:09 , might if I get a link for it or something?
100% noob question, if we had a plant that we wanted to get tc of(basically growing and ready to go) how much is that, and is there economies of scale for it?
Have you tried Tropica's aquatic plant tissue cultures.
Got the same experience on the commercial TC in germany. Maybe get some rare or variegated TCs from aquarium plants factory in the US.
Awesome video !
You should have washed the plants with a bleach solution before proceeding
It’s common to clean / sterilise plants
In a mild/moderate solution of bleach
Before adding them to aquariums or propagating them
If they weren’t contaminated (which they really shouldn’t be), they wouldn’t need to be rinsed with bleach
Como pones la pista de audio en español?
I can’t remember who but I think on Garden Talk with Mr.Growit a lady was talking about using bleach solution because alcohol can actually help some unwanted things (a type of virus maybe?) grow
This might explain the notoriously poor performance of the top fin tissue cultures. I thought I was going to be the exception to the rule with some of their bucephalandra but unfortunately it did not do well for me in the aquarium environment
Have Laur and Lujan ever been seen in the same room at the same time?
It’s interesting that the plants were noticeably contaminated only after they were subcultured. Is this to say that topfins sterilization technique is subpar and they are selling contaminated plants? Yet there is always a percentage of contamination
The bags they came in are not sterile. Alcohol will not sterilize, it kills somethings but not others. Spores are everywhere
❤❤❤❤❤❤thank you ❤
They're so contaminated! I see mites and scuds and stuff *thriving* in their media
EWW
@@plantsinjarswhat's wrong with these animals?
@frowner_and_co There should not be any animals in the tissue culture media
they're beneficial detritivores, but aren't something you want there if you didn't plan on it.
also they harbor bacteria, and some fungi parasitize marine amphipods. and as someone with aquatic isopods and invertebrate friends, I cannot abide!
so, it is a fake tc then?
The best tissue aquatic plant tissue cultures I have found are from ADA. Enjoy, aquatic plants are the best!
You had me there...
FYI petco has better plant selection.
Ayyyoooo daaaaaamn. I was excited to buy a shirt from the website but its $28 for shipping on a single shirt bringing it to $60! Is there a discount code on shipping??? (PS Make a hoodie please)
I need to make tissue culture of cumin and garlic Could you plz help me??
You need to do this again with not water plants 👀
I think TopFin only sells aquatic plants :(
@ do like a philodendron or monstera :p
Where are you seeing commercial sales of terrestrial plants in bags produced by tissue culture?
Can i buy the fruit fly from you?
🔥🔥🔥🔥🔥
It looks like it’s more of a pain in the ass than it’s worth I’ve bought a few tissue culture plants but they’re stupidly expensive compared to non tissue culture plants and I’m not payin’ that again
CLONE THE 🪰
Default level 1 no skin 😭😭😭
❤
Buke a flandra 😅
Its pronounced (roughly) Beu-sef-uh-laund-rah
I'm not judging, because I have no doubt I've mispronounced my share of scientific names over the years. Just trying to be helpful on account of all the aquarium keepers who are probably having fits now. 😂
Also, Tropica generally has very clean TC cups; you can store them for weeks on a lighted shelf while preparing a new tank. If I planned to get TC cups for starting my own multiplication cultures, that's where I would source from.
Top Fin stuff is cheap and often visibly contaminated, though as long as it hasn't completely overtaken the plants, that's not really a huge issue if you are dropping it directly into an aquarium (no special acclimation required; you open the bag, rinse under a tap to remove agar, and stick it in the tank). I buy it sometimes for my aquariums because it's readily available, but definitely wouldn't trust it to be sterile for cultures.
Thank you for taking pity on us aquarium keepers. ; . ;
Please its buuu ceee not bucee its killing me!!
Boose
Lol thank you. I pronounce everything wrong because I’ve only ever read and never hear the words IRL
That's super common for readers, like that guy who read all the works of "plah-to". Everyone knows it's pronounced "play-to" (but most haven't read Plato's works). Super informative video as always, thank you!
This intro made me run across my house and slap the fullscreen button.
And then you banned me from Discord, I thought that this was a place for tissue culture plants?
Or are you in fact working for big tissue culture now?
I'm just going to cry in a corner now...
Why were you banned and now crying?
@GreenCanvasInteriorscape because I posted photos of "abducted" or "poached" plants :(
But most of my plants that come from Thailand have all been propagated by my father who lives in Thailand and have been brought through TSA (airport security).
I understand that some people have problems with plants taken from other countries, but these plants are not protected in any form and you are allowed to bring them with you in sealed containers.
I also own a few plants that have been given to me by friends that just took a cutting in the hope I would be able to propagate it...
@GreenCanvasInteriorscape sadly I can't share pictures on TH-cam, but I have 39 different species of plant in my windowsills and a big grow light to give them 18 hours of light.
Lfg!!!!
Have you ever experimentaled with colloidal silver since it is antifungal antaiviral antimoldial and auntie bacterial. And it is great for human use as we all nose.
1:49 cute.
Such BS that you got demon-ized for just saying cannabis.