Tissue Culture Q&A
ฝัง
- เผยแพร่เมื่อ 24 ก.ค. 2023
- 2024 update: Watermelon Snow is now available on my Etsy! etsy.me/41V69lQ
Find me on Instagram: / plantsinjars_tissuecul...
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Protocol I mentioned in the video: www.researchgate.net/publicat...
Timestamps:
1:05 TC Timeline
3:53 Begonia tissue culture
8:03 Q&A
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
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Bro, this is what YT was made for. Love the channel
As a YT lady. I approve of this comment
i visited epcot maybe 10y ago and did the same tour it was amazing also i have been doing hydroponics and mycology work for some time which is now leading into aquaponics and tissue culture so am finding your channel very interesting and love that u are so beginner/hobbyist friendly keep up the good work and thank you
I'm an American plant junkie that's been living here in Thailand......If there are any plants you want from here...just let me know. I've been dying to learn tissue culture forever....so I'm your fan-boy! A very old fan-boy.
The part about you being able to do all this even if you studied finance really set in my mind that it's still not too late for me to be scientifically-proficient even though I didn't end up choosing a science major. I've always wanted to be a scientist but different factors led me to take a different route, and it hurts a bit to not be able to fulfill my childhood dream career. Anyways, I've only very recently discovered your videos and I'm binging all of them, and I really commend the comedic gen z humor your videos have while also being comprehensive yet succinct without being too intimidating for people who are new to this (like me)
Also, I might just be one search bar away from finding this all out, but I was wondering if you could make a video explaining why exactly the recipes for plants are different? If there's a pattern to it that we could take into consideration in case a plant doesn't have a recipe, and how we can adjust the ingredients to yield better results. Like why the recipes for a verrucosum and serpens are different, what they have in common, and how we could tweak the recipe for a plant that doesn't have one like the verrucosum x serpens (just using random names as an example)
Its so nice to find another person talking about begonias and african violets that isn't 3x older than me. Love the content!
Regarding C. sativa, you can always do a demonstration with industrial hemp. It grows like a weed (pun intended) here in Texas. It sticks out once you learn to recognize it.
Looking forward to the in-depth videos on organogenesis and somatic embryogenesis!
Theyre already available on her onlyfans lol 🤣
Underrated comment 😂😂😂
I'm joining the tissue culture journey! I ordered everything and more off of your starting list. 🎉
Keep me updated on how it goes!
Keep US updated ❤
i love your videos, and you have been my main motivation to open a small business using tissue culture
That is so awesome. Thank you!
Bringing down Big Plant ....
The empire is expanding...
Another amazing video, chock full of information. Every minute is educational, entertaining, or both!
You have the gift of teaching :)
Glad to see you’re hand is doing better. I’ve been there. Cut muscles and and tendons in my hand several times. Love plants and I love you teaching how to propagate!🌱
you are too cool for doing this series!
great lesson , thank you :) your every video inspiring me towards tissue culture
I'm all ears, your character and plant passion makes me tingle
Your videos have popped up a few times on my feed and I have enjoyed them. Brings back memories as I have a degree in Mycology (mushrooms) and a lot of what you do, I've done in the past. For those suggesting you do mushrooms, I say go for it. You can do tissue culture from most parts of the mushroom, just make sure you sample from an interior area protected from any contaminants. Standard medium is PDA (Potato Dextrose Agar) The recipe is available on lots of sites.
Personally, I would like to see you do it because it will highlight where you need to improve in your sterile work under the flow hood. PDA will grow all fungus, molds, and yeast so one small mistake will lead to a contamination of the plate. It took me 100's of plates to get my contamination down to below 2%. ;)
Hello @GrandpasPlace ! I am a frustrated marine mycologist, I say frustrated because my field is actually fish physiology. I wanted to shift to marine mycology but the institution I worked with did not allow me to. Nice to see a veteran mycologist here.
Fascinating, digestible and informative. A subject I have never been exposed to, but I am very much enjoying your tutorials. Also, love your philosophy.
I think you can reach that goal, rooting for you 🎉
You are so funny!
I am really impressed that someone with no science background has come so far in such a technical field. Having worked in a high end lab (virus culture, not tissue culture, but many of the techniques are the same), I am in awe at what you're able to accomplish on a shoestring budget. Keep up the great content!
I did have a question about tissue culture of patented plants....How big of an issue is that? My first thought when I saw your Watermelon Snow was that was probably a patented variety, but in looking through a bunch of sources, I don't see plant patents listed as frequently as they once were. Not sure how you know if the plant you have is covered by a patent.
By the way, accidental tendon damage like you experienced is absolutely no fun. Hope you continue to heal and get back to 100%. I had something similar happen a couple of years ago and my finger is no longer straight and refuses to contract as well as the others. Something you learn to live with.
Thanks again for your videos!
Hie my sister I really happy to see your practical nice works, God bless you
Hello Laur. Yes... I know you think I need a crash course in pop culture because I am on my forties and I don't know who is Hanna Montana. I'm absolutely loving that program from the University of Hawaii you told about. Preserving all plant species is so important, that every country should definitely hop on board before it's too late. Thanks for sharing your knowledge with me, Laur! I'm glad I get to learn all these amazing things with you! Some beautiful plants and flowers for you🏵🪴🌵☘🌺🌷😉😉
Great work! Thanks to you I I have been grow strawberry in tissue culture for a month now. I have only 15 jar, but they grow excellent!
im in love with you and your knowledge!
I just came across this video in my recommended, and when you read the question at 10:20, I was literally thinking about the Lyon Arboretum at UH Mānoa. They're doing really important work to keep the plants my ancestors once used!
It's just a shame we can't do the same for our endemic fauna--at least not in the same way.
Love your videos! Super interesting! :)
Whats your disposal protocol for media/disposable containers?
As soon as you mentioned mycorrhizal fungi networks I was like "oh yeah nice we'd get along great!", then you said you don't like mushrooms.
I'm not sure what to tell you now.
Jokes aside, I love this channel. I've learned so much. I've been propagating aquatic plants using various hydroponic systems, but I've been struggling with propagating enough of certain plants. I'm realizing this might be the answer to mass-production of babies to grow out, even if the initial time input might be significant. The scalability seems incredible! I'm also familiar with mycelium culturing and have the equipment and procedures figured out, so hopefully it transfers over well. Thanks for putting this information out there! I hope your finger's healed, I just watched that scalpel accident video.
Neat! First video of yours I've watched. I'd love to try it but my hands shake too much.
About schools: in 2014 I sold my business and went back to school. Now up to 5 degrees. Next is Ms in Biology and BS in Hort. You can do it too. Go for it. If you have questions, ask.
Thanks for the tree info, Much apreciated
Thank you for your amazing work! Here two ideas for future videos: 1) what about safety in using hormones and how to deal with disposables? Do you have to sterilize them after use? 2) from what I understand you are reproducing mainly indoor plants, what about making tissue culture with conifers? Is it possible?
I really like plants but could really watch you talk about anything 😊
This is fascinating.
Tammy would work possibly for the name 😆. Thank you for your content! I have been recently getting into this. I have several pothos, and monstera I am planning on TC 🙂. Thank you so much for what you do!
Love the way you present science, like a cool family member than a grumpy teacher. Also checkout ayurvedic plants for propagation they have a huge demand too !!
Yo you are an absolute gangster! Love your energy and information! Also, thanks for that link in the description 😅
I absolutely love that you’ve created a whole business out of this. I definitely assumed you had a biology degree of sorts, so double kudos to you for learning all of this yourself. I will say, I’m quite sad that you’ve switched to disposable containers. I’m not sure what the benefit is since you still have to sterilize them. And it’s just so much unnecessary plastic waste 😞. Nonetheless, love your videos and can’t wait to see the next one!
There’s allot of benifit in using plastic including not having to expose yourself to contamination to clean you just throw them out, at a exponential size it can be very expensive to invest in the same amount of mason jars and lids etc
I started tissue culture 2 years ago and I did tens of attempts and everything was a complete failure until today :(
But I never give up and I'm still striving to see a sprouted explant!
The interesting fact is that I don't have any fungal contamination (0% fungal) and the only thing that's ruining everything is endogenous and exogenous bacterial contamination from my explant! The last time I almost killed most of my explants in the sterilisation process but again I got contamination from the explants 😭
Try growing your plants that you cut off of sterile , grow with hydrogen or oxide, spray the plant with wet table sulphur to clean it with , before you take your cuts spray the plants down well with hydrogen peroxide/ water mix , 35 percent hydrogen peroxide at 10 mls a gallon. Then cut your material from that plant. This will help a lot getting your tissue through clean.
Hail the K-Cup tech 😁 I grow mushrooms and any savings I can use I take advantage of!
I found your content extremely informative. I look forward to your future content. I've only had a chance to view 2 of your videos. I looked at your etsy store and would be happy to shop there for rare tropicals, sorry not a begonia fan. I am assuming the Thia Constellation was tissue cultured and hope to find that in one of your videos. You had at least a 1/2 dozen including some Hoya, Burle Marx I am still in the market for. I have been successful with cuttings. Like I said I can't wait for you to finish your series. I subscribe to a few channels and would love to see weekly content. Too bad TC takes so long I came across an affordable batch of Spritus Sancti, but with my luck, the price will have dropped considerably before the plants are viable and I don't have the luxury of your weather living in MI. TC works for me with rarer plants because you can buy anything of real size here. A 4ft monstera is nearly $100 and if you try to ship larger plants they just get damaged. I put almost all my large plants on the balcony for the summer and they grow so well I don't care how much you spend on lights nothing beats mother nature. Come fall everything has to be treated as organically as possible for pests repeatedly I lost half my collection in 2 weeks at the start not keeping a close enough eye on thrips and spider mites. Now I know which big box stores sell plants that really attract them so I go buy some sacrificial ones that I really keep an eye on and place them just outside the main growing areas which has worked well and the plants can handle extra pest treatments better than some of the harder to grow plants. I am amazed that you didn't study botany in college. I do agree with you having shopped Thailand I came across a number of sites that had much cheaper shipping for tissue culture for rare tropicals. The one site would have shipped full-size mother plants for what you were charged. I guess I will have to sign up with a few social media platforms rather than just here if I am going to be able to follow you. I appreciate all the extra information you include like the names of a few places in the states doing tissue culture, where you can go to see it done. What better reason than to plan a trip to Hawaii.
LMAO don’t come for me at 8:36 😂😂😂
This is so very cool.
This is totally off topic, but you are SO CUTE and I love your hairstyle! 🥰
I would want more people like you in tissue culture since I want to find a way to get Wheat and Corn in hydroponics
We need you in the cannabis industry ❤ thanks for the nice content
Have you ever used tissue culture to remove plant viruses? Thanks for sharing all your TC knowledge!
So I damaged my tendon with MRSA and I can no longer do a good portion of things especially because it is my index. The tendon branches off. So it's important to use your hand. Keep at it 😊
You can try to grow a fluorescent mushroom it's very pretty
I couldn't find in your videos what kind of paper you use to make the micropropagation in the sterile cabinet. do you use normal kitchen paper or another kind? do you sterilize the paper in the autoclave? how is it done? thank you very much for your videos and your work!
STOP - not the Plants, Gardening & You class! My tomato plant was STOLEN off my porch and I ended up getting a B in the class because of it 😭😂 but I also absolutely loved that class and took it for no related reason to my major too lmao
Great video, looking forward to you next one on acclimation. Quick question, can you show close ups of a callus? I'm trying to TC citrus, and I'm concerned that a callus can look a lot like contamination...
Contamination will grow in the culture ,
You can compensate the mushroom eating with Soya Sauce. The chinese variety. *nod nod*
Nice work
Laur i love you 🥰🥰🥰🥰
Hi question: can you grow micro cultures from other seeds? You mentioned growing orchids that way and I'm only able to find rubus chamaemorus seeds even though I've been looking for a plant/cutting/bareroot for over a year
Thanks for the vid. What varieties seem to be most popular? Are you aware of any folks that do tissue culture for common edible plants, like herbs, fruits and veggies? Thanks. Cheers from N. FL!
I’m guna need you to upload way more often! Please. I need to learn all of this and I’m a binge watcher type lol I need all the videos 2maro
Such a neat TH-cam channel ❤🌱
I'm following you to do this with my cannabis cultivars I breed...
Nice to see you're focusing on b2b orders now. How's it going for you?
You may not personally like the taste of mushrooms, but you can make a profit selling them. Gourmet/rare culinary mushrooms and medicinal mushrooms can be grown from free materials like used coffee grounds from local coffee shops, or wood chips discarded by arborist companies. I know it's a lot of work running your own business, just an idea if/when you're looking to expend your horizons.
Just a question, what discord? And how do you clean the plant after it has rooted? How do you root it? How do you clean up the containers after you're done? I'm guessing you can't just remove the gels using water, since you need to specially treat it. How do you clean the tools?
I wonder, is it possible to tissue culture a pepper plant from a store bought pepper? I know with Aloha peppers if you plant the seeds from them, you just get plain yellow peppers. It would be nice to be able to grow those things at home
AYO Fellow Begonia lover! heck yeah!
Awesome...
One thing I never see mentioned is air during multiplication and rooting. Do containers need air hole and filter patch, and if so, what size hole. Only hole I have seen is on biocoupler.
Call it the Frankencutlure or saladculture jar, I'm leaning towards Salad culture🤪
If you decide to do woody plants. I have a small-ish pine tree thats worth growing for pine nuts. Would it be possible to try Pinus pumila?
Enjoyed the video
Can you keep them in there in the jars forever? And when they grow to big split them up? Or do they “mature” and need to be transplanted eventually
I grow mushrooms and find myself drawn to plant tissue culturing. I want to create a permaculture home someday.
Hi, I’m new to this. And you mentioned somewhere at the end of Part II, that on that big glass beaker or jar that the plants are contaminated? What do you mean by contaminated? And how do you know it is contaminated? How do I find out that my specimen is contaminated and with what? I’m just learning tissue culture from you. Thanks
For Kryo temperatures - USE LIQUID NITROGEN
I cringed when you hit your finger... I've only recently found your channel so the trauma is still very real here. Can I ask to what extent you need to be careful with waste containing the PGRs? The used media and waste water from washing the plants or washing containers, etc. How do you dispose of your troubled waste? Can't wait to watch you on this incredible journey.
Many thanks from Sydney, Australia.
Which light timer do you use?
If I only have room under my grow light for, let's say, 10 jars of my favorite plant/plants. How do I slowdown the growth? Thanks for all the info, it is literally like drinking from a fire hydrant watching your videos, lol!
Is there any reason why you use two explants? Do you sell or sources where I can buy the tools required in plant culture? Can I store explants for sometime before use?
😍🤗 sayin rooting lots of times sounds like a naughty Australian lol :D
Hi Laur. You mention that people from discord helped you for one of your begonia protocol. Are there any discord server dedicated to PTC enthusiasts that you know?
I'm curious if tissue culture would be a better way to propagate specific cannabis cultivars in particular specific phenotypes. Because traditional methods of producing clones, as far as I'm aware are by keeping one or multiple mother plants and taking cuttings then rooting those in rooting hormone but it is a process that often makes the plants more unstable in their growth pattern as I understand it, in particular not developing a vertical main stem that branches out evenly, but results in plants that have to be "monster cropped" and makes the care and training a lot more complicated and labor intensive. How does the quality of plants grown from tissue culture differ from cloning standard cuts? Does it affect the resilience differently? It's another topic of debate in the cannabis community that I haven't found a clear scientific answer to, some people claim cloning have the same exact genetics but others claim the more you clone the same plant the weaker the resilience gets. Isn't the exact phenotype preserved in all instances? Do the different means of propagation affect this differently do you know have any info on this?
Not plants in jail cells XD You could probably use non-active hemp plants used for fiber culture to bypass the law btw, just wouldn't be able to test flower quality
Is it possible to tissue culture a monstera albo? I know its possible for a Thai constellation but I heard something about the Albo being chimera or something.
Please helllp! 😭 I messed up!
I accidentally added 30gr/L of agar instead of 6gr/L. I used an expensive DKW media and other expensive additives and hormones and I don't wanna waste it!
Will this too high concentration of agar affects plant growth?
do you ever use coconut milk for cytokine, and what is the ppm like for your media. Whats you NPK structure for or do you use a sugar based mixture?
grafting is also fun in horiculture, almost the same process as inoculation
Imagine you can achieve success with something rare like Encephalartos latifrons going at around +/- $1000 per inch 😦 I must definitely try this out.
Try glowing mushrooms
What size are those deli containers you use??
Can you sterilize with an instant pot? I assume so just don't know how long to keep them in.
I love the reference of Edo Tensei. 🤭🤭🤭
Name the giant flask EMIL (or EMILy :) )...like the guy that it's named after, Emil Erlenmeyer. Love your videos btw keep it up!
Hey, can you please share the model and make of your Flowhood?
Is it possible to tissue culture a Hoya?
Will you do protoplast fusion someday?
How often do you get Contamination? It's funny in mycology Trich is contamination for mushroom cultivation but in the plant world it's used in mycorrhizal relationships. Any thoughts?
Usually around 10% or so get contaminated. Trying to get that number as low as possible though
how do you tell the jar has been contaminated?
There's some cool mushrooms out there some even glow in the dark
Ever tried aloë for propagation?
Your mom sounds lit. I also recommend everyone eat 1 mushroom a day.
Have you ever tried to TC carnivorous plants like Nepenthes, Drosera, or Venus Flytrap? What, if anything, is different? My concern is the disinfecting the explant material before culturing. They seem to die easily if they come in contact with a variety of substances. You have to use 0 ppm tds water to water the plant, so I'm curious how this changes with the nutrient you use.
I have sundews multiplying in some BioCouplers right now. The main difference is that you want to use less MS media (I think you use 1/3rd the amount you would use for other houseplants).
@@plantsinjars That's awesome. Thanks. Out of curiosity, what parts of the Drosera do you cut for your explant material? Also... what does MS media stand for? 😅
Your instagram link in the description isn't working. Just a heads up. Thanks for the videos
thank you! think i fixed it
Can you link your light timer?
Oops! Forgot. This is her: amzn.to/3Qv6kAH
You are cool) I'm wondering whether you have ever tried to grow clones out of buds? It's known that even if a plant is infected with any viruses or other deceases, the buds are usually free of those pathogens. It means that you can save a dying plant and make a lot of totally healthy clones out of it (which is impossible if you use leaves or stems). Some professional laboratories do it this way to propagate cultural plants such as strawberries or even potatoes and provide viruses free material to agriculture. I don't know whether this information has any value but I just wanted to share :)
If you have a flask that gets contaminated could you treat it with hydrogen peroxide?