Cell proliferation and cytotoxicity assay

[@zunikaamit]

Hi Julia, your video shows how to use the product with suspension cells. How about the adherent cells?

[@michaeljones2781]

Hi Zunika, Orangu works great with adherent cells.

[@shaymaabalamand]

Thanks so much.. very helpful ❤ Julia

[@FA-oz4cd]

Hi Julia, can you explain how do you generate your calibration curve?

[@scholarshipnetworkguideoff7988]

Great work done, I am PhD Scholar in China, Wish to work with you

[@twinbrothers111]

Thanks Jolia
Plz tell me how you determine LD50 plz

[@fran16justo]

Really nice!!

[@teferaworku8842]

Hi Julia I want to use the protocol that you have been demonstrated last time using column chromatography would you please! share me the the type of chemicals(reagent), column chromatography. And also would you send me the protocol and the required chemicals and instrument for the method. Thank you Julia

[@CellGS]

Hi Tefera,
The Exosome Isolation range protocols can be found here: www.cellgs.com/items/exosomes/exospin.html.
Please do not hesitate to email us at [e-mail] if you have any further questions. Thank you!

[@45bounty]

thk's so much..very helpful -)

[@luisp.9710]

Hi. Can this kit be used after 6 months (shelf life) and get reliable results?

[@michaeljones2781]

Hi Luis, Yes, the kit will still be reliable after 6 month's storage

[@dr.senthilkumarkalyanasund6099]

Concentration in 100microlitres of Sample is?.. Reply...please... thanks

[@CellGS]

Hi Senthilkumar, you can read the protocol here www.cellgs.com/products/oranguand8482--cell-counting-solution.html

[@biotical7697]

Hi julia
I Tried orangu assay for HCC cell line(Hep3B) , i seeded almost 7500 cells in per well(96 well) and it incubated , but it doesn't work i tried many times but i didn't get result do you have any suggestion?

[@michaeljones2781]

Hi I'msadaf, please contact [e-mail] for support

[@rjkim5000]

Nothing on quantified data analysis and results interpretation?

[@michaeljones2781]

Please have a look here. www.cellgs.com/exosomes.htm where we show examples of data generated using exosome purified using Exo-spin.

[@gogomahmoud6807]

From the videos i watched .the color converted from yellow to purple and here is become orange why?

[@kosheeka]

It depends on the dye you are using for your cell cytotoxicity assay.

[@lailatarar5885]

Hi julia can you tell me in detail about PFC assay mean to say how to perform it

[@CellGS]

Hi Laila,
The protocol for Orangu can be found in the following user guide: cellgs.e2ecdn.co.uk/Downloads/Orangu_User_Guide.pdf
Please do not hesitate to contact us at [e-mail] if you have any further questions. Many thanks.

[@dr.l.v.prasaddiagnostics6035]

WHAT IS THE DIFFRENCE BETWEEN Cell proliferation and cytotoxicity assay

[@michaeljones2781]

Hi Dr Prasad. A cell proliferation assay measures any increase in the number of cells. A cytotoxicity assay measures the capacity of cytotoxic compounds to cause cell death, so markers of cell death are measured.

[@kosheeka]

Cell proliferation assays measure cell growth, while cytotoxicity assays assess cell death caused by drugs or other factors. They provide insights into cell health and function, but target different aspects: growth vs. survival.