Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.
What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?
Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?
Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(
Because he is using anchorage-dependent cells, cells that only grow if they adhere to a surface. The cells are simply doing what they would do in vivo, inside the body. Cells that belong to tissues produce molecules that mediate both adhesion to the surface and cohesion with each other in order to actually form the tissue. On the other hand, red blood cells for example are usually in circulation in our blood so they wouldn't naturally adhere to a surface.
I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....
@@louisjacobmoon the basic idea of lab meat is to avoid killing animals, so if you are killing an animal to get Trypsin or something out of them so that the cells can feed-on and multiply then it defeats the purpose.
Many now use recombinant Trypsin eg TrypLE www.thermofisher.com/uk/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html?s_kwcid=AL.3652.3..e..o..tryple&ef_id=869904f24fa9112537fe6585ae811767:G:s&s_kwcid=AL!3652!10!76691033548341!76691086026937&cid=bid_clb_cce_r01_co_cp0000_pjt0000_bid00000_0se_bng_bt_pur_con&msclkid=869904f24fa9112537fe6585ae811767 However the foetal calf serum is most certainly animal-derived... There are some new but very expensive non-animal serum replacements
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Really great for someone who already has cell culture experience and just needed a quick crash course!
I can't agree more ! Exactly!
In other words, he is being waay to casual going about this to nitpick on. Haha
But still a great video.
Thank you so much this was really helpful. Going to crush my interview now!
This is the best video I have ever seen. Many congratulations, for having put your best effort into making it happen.
This is extremely useful, thanks Neil!
Excellent video, very clear and informative.
This is awesome, thanks! One note for anyone watching: Make sure you don't lay the pipette tip down with fluid still in it like at 11:05
Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross
Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,
Absolutely great
Excellent efforts, very nicely explained
I love your videos! thank you!
Great job 👏
véry good explanation sir
Very thorough love this
Thank you soo much!!!! That was brilliant
Thank you very much. This is very explicit.
Very nicely explained sir. Thanks a lot..
Very informative 👍🏻👍🏻👍🏻
Thank you
It was very helpful!! Thanks!
Thanks for this video👍
This is an amazing work! Thank you so much!!
Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?
Thank you this was very helpful!
Thank you!
Thank you for this nice video
REALLY GREAT .
thanks so much
Very good video , thanks
Very helpful 👍
Very nicely explained sir thank you very much 👍
this is so useful thank you so much for making this!!!!!!!
Thank u so much 🙏😊🙏🙏
An informative video
Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.
What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?
Very good presentation
Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?
extremely helpful
very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,
When you filling the 96well plate are you doing reverse pappeting?
Great video!
Nice sir it help us lot in COVID situation.
I serial dilution for that plating solution?
Thank you for this video, however I can't find the tutorial for counting cells :(
No trypandblue?😮
wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.
Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(
Can I ask if there are any cells in the dmem media that you throw out or all cells only adhere to the flask?
cells adhered to the flask
Is breathing on it adding potential contaminating?
Does this really follow aseptic practice? I doubt that。
Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?
Why he put 1.2 instead of 120 ? Someone explain please
How are the cells adhering to the flask enough so they're not dumped out with the fetal calf serum?
Because he is using anchorage-dependent cells, cells that only grow if they adhere to a surface. The cells are simply doing what they would do in vivo, inside the body. Cells that belong to tissues produce molecules that mediate both adhesion to the surface and cohesion with each other in order to actually form the tissue. On the other hand, red blood cells for example are usually in circulation in our blood so they wouldn't naturally adhere to a surface.
I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....
Sir explain how insulin cells are produced and insulin injection are prepared
Are any animals killed during the extraction of Trypsin from their pancreas ? Or can it be done without killing the animal ?
Why?
@@louisjacobmoon the basic idea of lab meat is to avoid killing animals, so if you are killing an animal to get Trypsin or something out of them so that the cells can feed-on and multiply then it defeats the purpose.
Many now use recombinant Trypsin eg TrypLE www.thermofisher.com/uk/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html?s_kwcid=AL.3652.3..e..o..tryple&ef_id=869904f24fa9112537fe6585ae811767:G:s&s_kwcid=AL!3652!10!76691033548341!76691086026937&cid=bid_clb_cce_r01_co_cp0000_pjt0000_bid00000_0se_bng_bt_pur_con&msclkid=869904f24fa9112537fe6585ae811767
However the foetal calf serum is most certainly animal-derived... There are some new but very expensive non-animal serum replacements
Finally found a cure for my son Sickle cell I’m so glad you came into my life. I pray for long life so you can save more souls,Thanks #druromi .
wow can you share your work or publications?
@@mrx4814 Yes sure