Such assays actually already exist. Here I only demonstrate a simple ELISA protocol aimed at teaching students the principle behind ELISA, but for clinical assays, the methods are often a bit more elaborate. You can learn more about how an ELISA assay can be used to diagnose SARS-CoV 2: www.epitopediagnostics.com/covid-19-elisa
Here I demonstrated how to perform an ELISA assay to test for the presence of antigens. But other protocols can test for the presence/absence of other ligands (i.e. protein) in a liquid sample using antibodies directed against the protein to be measured.
Great video man, very helpful. Thanks!
thanks from Marmara University, Turkey.
I have a question in my mind, why do we use towel for absorbance. isn't just spill the water into the sink sufficient?
Thank you!!
Why did the antigen stick to wall in the first step . Is it a special well ?
Thanks
Why can't we pippette out the liquid? Why to we have to dab it onto tissue paper?
What is gray zone calculations that is +-10%
When can we expect these kinds of tests for the COVID19 antigens?
Such assays actually already exist. Here I only demonstrate a simple ELISA protocol aimed at teaching students the principle behind ELISA, but for clinical assays, the methods are often a bit more elaborate. You can learn more about how an ELISA assay can be used to diagnose SARS-CoV 2: www.epitopediagnostics.com/covid-19-elisa
Why do u wash it the first time if there nothing to bind to it?
Wash the excess antigen that didn't stick to the well wall
when antibody and enzyme linked antibody binded with antigen which colour should be yield??
😢❤❤😮❤😮❤😅❤
what type of ELISA is this?
Here I demonstrated how to perform an ELISA assay to test for the presence of antigens. But other protocols can test for the presence/absence of other ligands (i.e. protein) in a liquid sample using antibodies directed against the protein to be measured.