Indirect ELISA

( www.abnova.com ) - The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies. More videos at Abnova www.abnova.com

[@MonsieurLavabo]

Holding the pipette vertical and pre-rinsing the tip will help you get more accurate results and reduce the variation between wells.

[@otienorose7674]

I like the way the video shows in details on how to carry out indirect ELISA test

[@heysup8889]

this video with that song was much of a life changing journey as it was an educational video, haha thx for the video !

[@ABNOVA1]

We don't have name for background music but we like it as well. Thanks for your support!

[@MonsieurLavabo]

@0102murphy No, the worst scenario would be cracking your plate if you're hitting extremely hard (which is unnecessary) but it is very unlikely for the Antigen/antibody bonds to break.

[@danielegalati1039]

excuse-me but after coating can we incubate at room temperature for 3 hours or 4°C overnight for wichever proteins? Or this incubation is specific for protein that you're working in this video?

[@vonguoinoithang]

Thanks for video! It gives me more information about indirect ELISA tech.! Very useful!

[@MonsieurLavabo]

@MalagaJolu No, it really depends on the assay you are doing. For example it can be an antibody in Blocker Casein/PBS.

[@0102murphy]

Will the coated antigen in the plate being shaked out if you hit the plate against the table so vigorously??

[@1993LAlice]

What is the purpose of sealing the plate with parafilm? Thanks!

[@ManuelOrozcov003]

OK well, but Cual es la función de cada reactivo utilizado en ese experimento? : )

[@shaymaaali6213]

thank you

[@thepoohbearful]

NEED HELP ASAP! WHICH ELISA TEST SHOULD I USED TO CHECK THE PREVELANCE OF JOHNES DISEASE IN CATTLE?? ILL BE USING BLOOD / SERUM SAMPLES. AND WHAT KIT # ALSO?

[@MalagaJolu]

the coating buffer allways have to be 0.1 M Carbonate pH 9?

[@assiadj7450]

could u help me plz !!
we need to know the cytotoxicity of Macrophages and PBMC which are stimulated with LPS,PMA/PHA .. so we used MTT test and after to detect absorbance, we used enzyme linked immunosorbent assay(ELISA) plate reader at 570...
how we did this ELISA test !!??

[@ArnavPadhi]

Why antigen needs to be diluted in coating buffer??

[@8t1c0f1s9h1]

what is the name of the music?

[@benderadan]

nice one very advantageous

[@Tepmodify]

I like . Good technic

[@justdeadnow1404]

11 years ago ? dayum

[@infoghinaztv5773]

thanks . 👍👍

[@martinmwandiki7000]

I like the video

[@shomeshali5675]

Nice

[@AkumaHaji]

thx u for viedio . tomorrow i must do elisa T_T for project . this viedio i understand >^

[@sohinisen7512]

After coating antigen, why do you add the blocking buffer?

[@mohammedahmedjalloh531]

to prevent unspecified binding

[@joshj747]

Song is a banger a 2x speed

[@MalagaJolu]

Ok, for example I have my purificated Ag ( I want to perform a indirect ELISA) in Tris buffer 50 Mm, 100Mm Kcl, do u recomend me hibrid my antigen to the microplates with that buffer?

[@caleb8781]

Interesting

[@alexandrasoriano542]

why does the song go crazy at 1.75x speed

[@SammyMageto-db7xw]

By now Im able to know direct eliza