This is absolutely the BEST, MOST CLEAR, STRAIGHTFORWARD VIDEO I have ever had the pleasure of learning from! Thank you for making my day! College science should ALL be done with videos like this one
Thank youuuu! I missed a biochemistry lab and I couldn't have done my practical exam without understanding this fully. Your explanations are so easy to follow. My lab book is covered in notes now :)
I was going to wait for the video to at least get 1/3rd of the way before I was going to like it or not, but the Standard Curve definition and example with the consistently-themed penguin objects made me lol and informed, you deserve a like/sub!
Literally I watched and asked so many videos and people about this topic but still there are some lingering questions in mind. But this video cleared everything within 7 min. Tqsm
Thank so much ma'am....that's topic make me so difficult to understand because already miss lectures by professor but now I totally got understood What exact standard curve is.....thank you again
Thank you so much! You did an amazing job of breaking it down...I've been in Molecular Biology class for a few weeks and during lab, the professor didn't bother to describe what a standard curve was. I was thinking, "where is the curve?!". I get it now, thanks to you.
Excellent - clear, concise and educational! 2 questions, 1) How do you know what wavelength to use? 2) What is the difference between a spectrophotometer and a ELISA reader?
WOW! Thank you from the UK! My assignment is based on protein concentration in samples. This video explained it perfectly. I was clueless before I watched it! THANK YOU!!!!!
Thank you so much, I had to use a standard curve for something in uni and I had no idea what I was doing haha This is so clear and I finally understand it!
I just fell in love with you. You just made it so easy. I spent all day trying to understand the graph of ELISA, but I was stuck on standard thing. Now I've got it thanks to you :D
Glad I came across your video I now have a better understanding of this procedure. But as an insipiring analytical chemist, can you ever detect 0 ng of "stuff" or whatever is being sampled?
Could you also just dilute the sample 1:2 and then calculate the concentration? And thank you so much for the video!!! I finally understand clearly how it works!
It depends on the solution, [FeSCN]^2+ for example is a reddish color solution, while Fe^3+ and SCN^- are essentially colorless. Since FeSCN^2+ reflects red light it absorbs BLUE light, so you find a wavelength in the blue light region! (I know this is a 5-year-old comment, but I just came across this video, hope this helps anyone!)
Hi. The best explanation so far. But I have a question. What happens if the concentration that you got is above the maximum concentration? should you accept or not? and What must you do so the concentration must be within the calibration curve? I hope you'll reply
Well done and thanks for this amazing video lesson. Great explanation, If you permit me I will use it for my students in Biochemistry class. My regards
![กี่หมื่นฟ้า | Your Sky Series EP.1 [2/4]](http://i.ytimg.com/vi/qJbQs_nhjD4/mqdefault.jpg)
In less than 7 minutes, you've explained everything in lecture I zoned out on, omg thank you!!
This is absolutely the BEST, MOST CLEAR, STRAIGHTFORWARD VIDEO I have ever had the pleasure of learning from!
Thank you for making my day! College science should ALL be done with videos like this one
I'm so glad you found it useful!
instablaster
Thank youuuu! I missed a biochemistry lab and I couldn't have done my practical exam without understanding this fully. Your explanations are so easy to follow. My lab book is covered in notes now :)
+Francesca Hewitt I'm SO HAPPY!!! Thanks for watching and letting me know!
Kathryn Francesca what your University specialization??
I dont understand this curve in medical lab
Simple yet powerful explanation on stamdard curve and dealing with out of range samples.
this was generally one of the best videos I've ever come across, thank you so much!
Thanks so much! Glad it was helpful.
THE BEST AND CLEAR demonstration
Thank you so much! Glad it was helpful.
I was going to wait for the video to at least get 1/3rd of the way before I was going to like it or not, but the Standard Curve definition and example with the consistently-themed penguin objects made me lol and informed, you deserve a like/sub!
I'm so glad to hear it - thanks so much!
6 years later and it's still a helpful vid , thanks miss u just helped me pass my medical interventions exam
SO happy to hear that! CONGRATULATIONS!!!
Oh thanks! Definitely helped me to understand for my biochemistry lab report!!
Literally I watched and asked so many videos and people about this topic but still there are some lingering questions in mind. But this video cleared everything within 7 min. Tqsm
Such clarity, that too in one shot🙏 wonderfully explained, Mam
Thanks 🌸
So kind of you to say! Glad it was helpful!
OMG, I've struggled my whole life to understand this and you just made it so easy and simple. Thank you!!
Yaaaassss!!!!!
you're so kind i was desperately looking for this. thank you so soooo much!!!!
I'm so glad you found me! You are sooooo welcome!
I have been struggling to find the values watched so much stuff but youu explained it soo smoothly god bless uh🌈⭐
Thank so much ma'am....that's topic make me so difficult to understand because already miss lectures by professor but now I totally got understood What exact standard curve is.....thank you again
Fantastic video, I understood everything perfectly. The ruler example made it all click in my head, thank you so much.
You're very welcome! I'm so glad it clicked.
I will definitely subscribe. You are amazing explaining. Thank you so much.
Omg thank you so much for this knowledge you saved my academic life
Wow!!! You are so welcome!
Loved your explanation! Didn't have slightest idea in class what the standard curve is... Thank you!
Excellent video, i had made lot of mistake and extended the x axis and y axis . but you have discussed it very clearly.thank u so much
Glad it helped!
Thank you so much! You did an amazing job of breaking it down...I've been in Molecular Biology class for a few weeks and during lab, the professor didn't bother to describe what a standard curve was. I was thinking, "where is the curve?!". I get it now, thanks to you.
Fantastic and simple explanation for those of us who are assay beginners!
You are absolutely amazing!! God bless u!!
Excellent - clear, concise and educational! 2 questions, 1) How do you know what wavelength to use? 2) What is the difference between a spectrophotometer and a ELISA reader?
WOW! Thank you from the UK! My assignment is based on protein concentration in samples. This video explained it perfectly. I was clueless before I watched it! THANK YOU!!!!!
My online course is using this video as learning tool. Thanks :) Your video really helped.
Thank you so much, I had to use a standard curve for something in uni and I had no idea what I was doing haha
This is so clear and I finally understand it!
Thank you! So easy to understand my assignment now! LIFE SAVING work done here today. Thank you Penguin Prof!
Glad it helped! You are most welcome!
You really make it easy to understand, thanks a bunch.
+Po Ho You are so welcome - thanks for watching!
thank you so much doctor
this video was so smooth to the brain
Smooth on the brain is a great compliment! Thanks!
BIOTECH TEST TOMORROW AND I AM SO READY THANKS TO YOU!!!
+Saul De Los Santos So glad to hear it! GOOD LUCK!!!
You are the best!!! This is a really good contribution.
Thanks for watching!
So clear and simple! thank you
Nice and clear explanation! Thanks!
Glad it was helpful!
Thank you, you explained very clearly.....
Wow!!! no one can explain better than you!!! awesome presentation!!! Thank you so much!!
Really good brush up lesson, cheers :D
very well expalined.thank you.please make a video on TPC protocol and calculation
Or you can perform single/serial dilution, so that the unknown concentration falls within the 'ruler'?
Excellent advice and methodology for making any measurement. Thanks!
thank you for your effort , its very simple and clear
couldn't understand when my teacher taught me, but I got it first try when you did, thank you!!!!!!!
Really great video I didn't understand it in class and this video was so helpful and easy to understand. Thanks so much!!!
I just fell in love with you. You just made it so easy. I spent all day trying to understand the graph of ELISA, but I was stuck on standard thing. Now I've got it thanks to you :D
I love you too! Glad it helped. You realize you're dating a penguin now. Just to be clear. :)
+ThePenguinProf I'm so glad I am 😁
Thanks. It made it easier for me to understand it.
thank you....easy to understand and very helpful
Nice presentation I really impressed good work thank you mam
Thank you for watching! Glad it was helpful.
I love this video i ask for more: coefficients of variance. And controls meaning vs calibrators please.
Glad I came across your video I now have a better understanding of this procedure. But as an insipiring analytical chemist, can you ever detect 0 ng of "stuff" or whatever is being sampled?
Tbh.. this is the best video for explaining this.. finally I understood what's going on
I'm so happy to hear that! Thanks for letting me know!
fantastic video!!
ELISA seems complicated but not at all! Thank you ThePenguinProf!
Kisses back! Good luck to you!!!
Thank you so much for your detailed lecture.. I just loved it. It provides all basic answers of mine about this topic.
Good luck 😊
Could you also just dilute the sample 1:2 and then calculate the concentration?
And thank you so much for the video!!! I finally understand clearly how it works!
It's a complex relationship, so don't dilute and calculate... dilute and measure the ABS. Be safe. Glad it was helpful!
you cleared all my doubt in just 5 mins ..thank you
Thank you, this is very informative
You are a LEGEND! Thank you so much!
Awww... thanks so much for the comment!!!
straight to the point thank you!
You've just made my life a bit easier. thank you
Woo hoo!!! Thanks for letting me know!
It helped me a lot. Thanks 🙏
Glad it helped!
Great great video, so much easier to understand
Glad you think so!
Thank you so much! I really needed this clarity!!!!
You are so welcome! Thanks for watching!
Your video is amazing.
Thank you! Glad it was helpful.
Thanks a lot. Really helpful.
May god bless you.
You are most welcome! Thanks for watching!!!
very helpful and very cute penguins!! thanks so much!
+Abby Jutilo You are so welcome! Penguins make everything easier, right?
How do you know which wavelength to use?
It depends on the solution, [FeSCN]^2+ for example is a reddish color solution, while Fe^3+ and SCN^- are essentially colorless. Since FeSCN^2+ reflects red light it absorbs BLUE light, so you find a wavelength in the blue light region!
(I know this is a 5-year-old comment, but I just came across this video, hope this helps anyone!)
Thanks a lot! You explained it better than my professor🤙
Happy to help!
Amazing! More videos like this please!
This is very clear, thank you.
Glad it was helpful!
Thanks maam , it really helpful , if the profs in our school can do the same lecture as u did , i think all of us will love to study in this course!!!
Terrific, terrific...thank you.
wonderful explanation,
So glad it was helpful!
Hi. The best explanation so far. But I have a question. What happens if the concentration that you got is above the maximum concentration? should you accept or not? and What must you do so the concentration must be within the calibration curve? I hope you'll reply
If your unknown concentration is above the standard curve, you need to make new (higher concentration) standards -- never extrapolate!
Thank you so much from Africa, i just subscribed. That biochem lab won't know what hit it.
Wow mam u r excellent ❤️ thank u for clearing my confusion.
I'm glad you found it helpful!
Legend! Thank you! So easy to follow and understand....
Well done and thanks for this amazing video lesson. Great explanation, If you permit me I will use it for my students in Biochemistry class. My regards
Thank you for watching and sharing! I hope your students also find it helpful.
Oh god. I needed that! Wonderful
SO glad you found it helpful!
Thankyou you're a saviour ❤
So happy it was helpful! 🐧
Very helpful! Thank you so much!
You're very welcome!
That was extreeeeeeeemly helpful
I am soooooooo happy to hear that!!!
Thank you is the most that I can say tbh! what an amazing video!
Wow, thank you!
Ma'am you are amazing ❤️Please make more videos like this😘
Thanks so much! I will!
you are really amazing easy explanation >>thank you so so much
+sufian algohary You are welcome! Thanks for watching!
Ahhhh. I understand this now. I didn't in my class. Thank you very much.
Thanks you made it so much simpler than my professor did!!!!
Very Helpful!
Great Video! Tkank you so much! Best regards from student from Poland ahhaha!
I'm so glad it helped! Thanks for watching!
Thank you Profpenguin!
thank you so much for this nice explanation. I really appreciate that!
I loved this - well done!
thank you so much, it is very helpful
I'm so glad! You are so welcome.
Thanks so much. You are brilliant!!
Best explanation
Glad you think so!
Thanks you so much that helped so much.
this is amazing! thank you so much
You're so welcome!
i love ur lectures!!! so helpful
Nice explanation
Glad it was helpful!
So enlightened
So happy to hear it!
Made that look easy ☺️