Generating Standard Curve and Determining Concentration of Unknown Sample in Excel - Easy Method
ฝัง
- เผยแพร่เมื่อ 20 ก.ค. 2024
- In this video lecture, we explain about
Generating Standard Curve and Determining the concentration of Unknown Samples in Excel.
-Processing absorbance data
-Plotting concentration vs absorbance scatter plot
-Adding Trendline
-Displaying R2 value and equation on chart
-Calculating the concentration of the unknown sample such as protein concentration from the absorbance
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#Biologylectures #biochemistry #protein
Thank you so much for this presentation !
You are most welcome 🤗
Excellent this is what I was looking for.
Glad it was helpful!
Best lecture on TH-cam ❤
thanks
That was very helpful, thanks a bunch
You are most welcome 🤗
Thank you so much, sir. It was well presented with a moderate pace and exceptional explanation.
Thank you very much for the kind words
Thank you very much for the kind words ❤️
Super amazing, you made it so easy
Thank you very much 😊
Thanks for all of your wonderful videos, I'm just wondering if you have any video here to measure growth curve and how to do that experiment under inducer effect if you have it already please paste the link here I could not find it myself.. best
You are most welcome. Please find the link below for the bacterial growth curve. We will upload another video about how to measure the bacterial growth curve over time as soon as possible. th-cam.com/video/U66FloGr-s0/w-d-xo.html
Dude you saved me!! Thankyou so much this was so helpful
You are most welcome.
thank you sooooo much for this..... very simple....
You are most welcome.
Thank you very much!
You are most welcome 🤗
Excellent tutorial
Thank you! Cheers!
This equation is used for testosterone analysis ?
Thank you🙏🌹❤
You are most welcome.
you saved me. thank you so much
You're welcome!
How do I convert the concentration gotten from the graph to percentage
thank you so much :)
You are most welcome
Did you measure two times on the same same samples ?
Yes, that is correct. That's why we have technical duplicates.
how can you find an unknown dosage using the final concentration and the absorbance?
Great!!!
You are welcome.
Easy to understand
Thank you
How did you get the equation
As shown in the video, click on show equation icon
How to convert mg/ml to mg/100gram... Please reply
Is it must for my plot points to be on the line?
Not necessary..... points may be above or below or on line....r value vary accordingly
aoa
reading of absorbance was measured on ------ nm ??
you did not mention it
kindly explain it
thank you
You can measure the absorbance at 562 nm.
@@BiologyLectures thank you
@@biyamushtaq9616 You are most welcome.
Concentration sy phly wali absorbance kha sy i
The Concentration I got from the Spectrophotometer and from this equation are totally different.
Then please follow the instructions shown in the video to calculate the concentration
The equation will be x = (y- intercept)/ slope.
U are doing x = ( y+intercept)/slope
Please explain if i am doing wrong
I have one doubt that the concentration of unknown will be in mg/ml or mg/100 gram?
It will be in mg/ml.
How i come Germany ❤😊
You can come to study or work in Germany.
@@BiologyLectures Tell me the process please
@@BiologyLectures can you call me..?
@@BiologyLectures Respected Sir..Can you please help me out to find the other value? I have absorbance value of unknown sample for different time like 20 min.,30 min. and for 60 min. I have standard curve also for the same drug. I have used D-glucose and the process is In Vitro Reverse Iontophoresis Process. Now I'm struggling to find the other value. What I want to calculate: Extracted glucose Concentration (µM), Concentration for receptor chamber (µgm), Cumulative amount(µg), Glucose flux extracted(nmole cm−2 h−1), steady state flux, cumulative penetration for each sampling time.
Can you please share your mail i'd here..??