Very nice presentation. A few items to add: Since the blank represents 0 concentration and 0 absorbance, you can plot that as a sixth data point (0,0). In fact, that is why the best-fit straight line (as yours appears to) should begin at the point where the axes meet. Also, I would point out that making sure the best-fit line leaves about as many data points above it as below it (as you did) ensures that this line is "averaging out" any imperfections in the measurements.
This is an absolutely fantastic video!! I thoroughly understand the concepts of the lab now and am much more confident going in tomorrow! Thank you so much you were such a huge help.
It would, but for this particular class we are simply introducing the concept of how protein concentration and absorption relate to one another, as well as how a standard curve could be constructed from that obtained data. Beyond that, many laboratories still do perform standard curves alongside protein assays, so this is intended to provide the students with the protocol associated with those assays.
Thank you so much for this video! my professor didn't answer my question about standard curves, but this video most definitely did! You are an excellent professor! thank you!
Thank you for this video! I been trying to solve this through Beer's Law formula and only lead me to deeper confusion. Plotting my points and finding the X-axis with absorbance number is much easier.
Help with a question ????? A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0,6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345,79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.
god bless you and your family, you have no idea how much help you provided for us. may the reward of this help be paradise in the afterlife and a lot of happiness with prosperity and good health in this world for you and your family. Amen
Thanks and well explained in conceptual term. I would like to ask that if possible, please show the use of spectrophotometer because new researchers faced tedious problems particularly when no Lab technicians are available.
If I have a standard solution of 5.0 ug/ml P and want to make dilutions of 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, and 5 mg/L to make a standard curve to determine mg/L P in a sample how would I prepare those dilutions ?
Oh my goodness. Young man, whoever you are, God bless you. This finally makes sense.
Amen
You just saved my life for my uni assessment!!!!
Happy to help!
Very nice presentation. A few items to add: Since the blank represents 0 concentration and 0 absorbance, you can plot that as a sixth data point (0,0). In fact, that is why the best-fit straight line (as yours appears to) should begin at the point where the axes meet. Also, I would point out that making sure the best-fit line leaves about as many data points above it as below it (as you did) ensures that this line is "averaging out" any imperfections in the measurements.
How wonderful this was! It cleared SOOOOO many concepts up for me.
Thank you so much!!! Three years and counting and this video is still helping people. I got such a better understanding!!
This is an absolutely fantastic video!! I thoroughly understand the concepts of the lab now and am much more confident going in tomorrow! Thank you so much you were such a huge help.
It would, but for this particular class we are simply introducing the concept of how protein concentration and absorption relate to one another, as well as how a standard curve could be constructed from that obtained data. Beyond that, many laboratories still do perform standard curves alongside protein assays, so this is intended to provide the students with the protocol associated with those assays.
Thank you so much for this video! my professor didn't answer my question about standard curves, but this video most definitely did! You are an excellent professor! thank you!
finally someone who explains it like it isnt rocket science.
Thanks alot. I've been searching for such a video for three days. It was very helpful.
I see how this relates to accuracy and precision ideas, thank you
Happy to help!
Thank you for this video! I been trying to solve this through Beer's Law formula and only lead me to deeper confusion. Plotting my points and finding the X-axis with absorbance number is much easier.
Happy to help Ali! Cheers.
Thank u so much , I can't wait to subscribe, this video explained everything I needed to know and fill in my report
Thanks for the sub, and I hope that my videos continue to help you!
Dude I love you. Thank you, you make everything make so much sense!
You are very welcome!
Help with a question ????? A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0,6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345,79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.
god bless you and your family, you have no idea how much help you provided for us. may the reward of this help be paradise in the afterlife and a lot of happiness with prosperity and good health in this world for you and your family. Amen
Gob bless you man for making it easy for us.
It's like simple Linear Regression, now I have idea, thank you
man can u explain it with a y=mx+c equation.?
God bless you
You just solved my assignment
Good refresher!
you my friend are a lifesaver
Thank sir .what i want to know is if i can use either beer's law to find the unknown concentration for a given absorbance or from the graph
Great thank you sir.u are best teacher,help us lots
Thanks and well explained in conceptual term. I would like to ask that if possible, please show the use of spectrophotometer because new researchers faced tedious problems particularly when no Lab technicians are available.
Excellent. Thank you!
You are welcome!
Thank you!, that was very helpful.
Gardenia Magnifica Happy to help!
If I have a standard solution of 5.0 ug/ml P and want to make dilutions of 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, and 5 mg/L to make a standard curve to determine mg/L P in a sample how would I prepare those dilutions ?
thank you so much for your wonderful explanation!
Can we convert the concentration values to absorbance values lyk how we concert transmittance to absorbance values
How did u get the absorbance?
would the results be not as accurate if most of the other points were not on the line?
Simple and easy, good in u Mark...
Very helpful.But how we want to calculate by using formula?
Thank you .that was very helpful.
Extremely helpful 😊
Which data we must put in x, and which one in y?
Thank u so much for making this excellent video!.
I'm glad that it helped.
excellent video
Thanks a million sir!!
Wow I loved that lecture. We'll explained.
It is compulsory to calibrate the spectrophotometer by adding a Blanc tubs!
Great explanation!
جزاك الله خير . جدا استفدت من شرحك
Very helpful!!
Well explained. Well Done
Thank you, this makes sense now!
You are welcome! I'm happy that it helped!
Thanks sir it helped me alot
Sir if hav any artical on this plz send me
Thanks so much
You're welcome!
You are quite welcome!
could you plz share videos about drop count for bacteria ???
So helpful! Thankyou!
You are quite welcome!
Thanks 🙏 Much appreciated
It's really helpful ,thankuu
May God pay you withwhat u deserves best Sir
Easy to understand..thanks bro
would it not be .39 instead of 3.9
That was helpful thank you
How to calculate with equation
Thank you sir...
thx for making this simple
thanks .... but in this way we use beer lambert law or not ...
Thank you so much
Thank u so much sir!!! My chem Professor is no help😭😭.
Thank you very very much!
Thanks a lot
Thank you mark
life savior
Very useful thx
Thanks!
Lace Miggins ..hey
Why is it called a standard curve when you're drawing a straight line? Couldn't you draw a curve of best fit instead of a line of best fit?
nice sir thank u..
mark, you beauty
How ican do it math not curve
❤️💋
How is it a standard curve but you're drawing a line of best fit
شكراً دكتور عاشت ايدك دكتور الله يطيح حظك دكتور 🥬😂
How did u get the absorbance ?