Feeding Paramecium with stained yeast

Paramecium caudatum is a well known ciliate and model organism in biology . Its comparatively large size make it easy to observe its internal features. This video shows the digestion process in Paramecium using bakers yeast that was stained with congo red - a pH indicator.
A small amount of fresh yeast is dissolved in water and the solution is brought to a boil to disrupt the cell wall of the yeast. When the solution has cooled down, a few drops of a 0.1% congo red solution is added to the yeast. The yeast take up the dye and a small drop can be added to our ciliates which will readily phagocytose the yeast. During the digestion process, the food vacuole is acidified by proton pumps which activates the digestive enzymes. Due to the acidification process, the yeast stained with congo red turn blue.
Microscope:
Olympus Vanox AHBT3 with DIC
Objectives used:
Olympus SPlanApo 20/0.7
Leitz NPL Fluotar 50/1.0 Oil
The camera is a Panasonic S1 adapted with an Olympus NFK 2.5x eyepiece.
Music:
Fern - Ann annie

[@alexandrevaliquette3883]

I would suggest you to show a small image of you doing the preparation. It's always nice to have visual support.
What a great image quality you have by the way!

[@DuhKodaa]

Back again! Always nice to see you upload, great footage and info per usual :)

[@VANOXmicroscopy]

Thank you Brian!

[@Microscopyenthusiast]

Welcome back! He is alivee!😀

[@VANOXmicroscopy]

Haha of course :D

[@alexandrevaliquette3883]

What a great idea!!!

[@trr7fd]

Amazing video, you deserve way more subscribers

[@SLAUGHTEREDEYES]

He returns !! good stuff my guy!

[@VANOXmicroscopy]

Thank you :D Happy to see that my trusty subscribers are still here :)

[@SLAUGHTEREDEYES]

@@VANOXmicroscopy Im here as long as you are lol.

[@VANOXmicroscopy]

@@SLAUGHTEREDEYES

[@ElGeologoModerno]

Which Paramecium species is this? Thank you.

[@VANOXmicroscopy]

It is most likely paramecium caudautum!

[@ElGeologoModerno]

Thank you.