Fungi infected Oligochaete - Pristina sp. under the microscope (DIC)
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- เผยแพร่เมื่อ 7 ก.พ. 2025
- The Oligochaete from the genus "Pristina" (maybe Pristina longiseta) originated from a hay infusion and was heavily infected by a fungus which bevomes evident by the huge number of spores inside its body. It's body is also covered by Cilliates which could belong to the genus "Vorticella".
The genus Pristina belongs to the family "Naididae" and contains 21 species.
An Olympus Vanox AHBT3 microscope was used with differential interference contrast. The UVFl 10/0,4 and SPlanApo 20/0,7 were used together with a Panasonic S1.
Thank you all for the positive feedback! I really really appreciate it ! I already have a long list of upcoming video ideas so stay tuned and stay healthy :)
Ma favourite Microvideo I've seen so far! The quality is breathtaking!
Thanks Vanox, quality as always!
Thank you BTNE !
Amazing montage! Very fine and clear definition and contrast plus a great subject. Subscribed!
Thank you!
@@VANOXmicroscopy If its not a secret would you mind telling me how you connect your Panasonic S1 to the microscope? Is it via afocal or direct projection and what sort of mounts and adapters did you use?
@@microscopyoptics2881 Not a secret of course: I use the 2,5x NFK projection eyepiece from Olympus without additional optics. There is only the Olympus L phototube between projective and camera. The Olympus L phototube however needs an adapter that converts the OM mount to the Leica L mount. Thats basically the normal adaption you use for all finite Olympus microscope from the BH-2 era.
@@VANOXmicroscopy Thanks a lot!
Absolutely interesting
Thanks, I like your videos :-)
@@VANOXmicroscopy Thanks!
awesome footage
Thank you Boris :)
Excellent DIC microscopy and photomicrography! I and my son posted a video of a naididae some days ago. Polarization. I have, unfortunately only DIC for 40x and higher...
Thank you! I will check it out :)
Amazing quality and clarity. What objectives do you use?
Thank you :) I used an Olympus SplanApo 20/0,7 objective for this video. Probably my favourite objective. I also used the Olympus UVFL 10/0,4 for the lower magnification shots
That microscope has killer resolution.. i have a swift 1800 from an school lab u think that thing could ever look this good??
I'm happy that you like the quality :) I just googled your specific microscope but couldn't find a picture. Swift microscopes are entry level however, that doesn't mean that you can't have lots of fun with it ! The clips from water organisms i produce are usually in DIC (differential interference contrast) and only high end microscopes from the big 4 brands can actually be equipped with it (and it costs several thousands of dollars). The microsocpe I use is from Olympus and more than 30 years old (thats where DIC becomes more or less "affordable"). If you want to get somewhat similar results with a certain 3D effect you should try to do oblique illumination. You can DIY that yourself !
@@VANOXmicroscopy woooow cool! 🤤
Amazing! Did you find this in your own culture ?
Thank you! Yes I have a very old hay infusion (couple of months old) next to my microscope which i occasionally feed with a drop of milk. There are a couple of them in there and all of the ones i have seen so far, seem to be infected.
@@VANOXmicroscopy so cool! I've just recently bought my first microscope. I'm a student and I'm pretty comfortable with a microscope but have 0 experience of growing and finding cultures. Have any tips on starting out a hay infusion? Would love to find something like this
@@vincentdandolfi2362 Congratulations to your new microscope, I'm sure you will have a lot of fun with it :) I'm a biology student myself.
Getting a hay infusion started is pretty simple: Get some dry grass/hay, put in a suitable container that allows gas exchange and cover it with water from a natural source (pond, river etc.) Make sure you not only take crystal clear pond water but also some detritus and decaying plant material from the pond (thats the place where most organisms live). Put your hay infusion at about 20°C (room temperature) and wait a couple of days. After a week you will have lots of microorganisms but be sure to check every day and observe the progress because the dominant species you find will change over time. Good luck and don't hesitate to contact me if you need more help :)
@@VANOXmicroscopy thanks! Any tips on the container ? Because I'm sure it will start to smell haha,
@@vincentdandolfi2362 I use plastic containers or glass jars for it. I try to make the hole for gas exchange not toooo big because as you already mentioned, it will get a little smelly ;) Maybe you can find a place where the smell will be acceptable. So far, it's the best method I know of to have a steady supply of something to look at. I don't have too much time to go to the nearest pond whenever I want...student problems ^^
Awesome! What song is playing?
Thanks :) The song is called Simple by Patrick Patrikios. I got it from the YT music library
beautiful stuff .. what scope / lighting you using ?
Thank you :) The microscope is an Olympus Vanox AHBT3 with differential interference contrast (DIC). I changed the original halogen bulb of the microsocpe to a powerful LED.
@@VANOXmicroscopy would it be possible to make a vid of the scope and how you set up the dic?
@@SLAUGHTEREDEYES That's totally doable! It will take some time however as i think the video would be a little better with a voiceover but the microphone I ordered hasn't arrived yet. So stay tuned :)
@@VANOXmicroscopy Awesome.. I have searched high and low for any kind of info on how to align the prisms or anything useful on dic other than describing what it is. I'm just starting but I try to research any methods I can to increase my little scopes abilities. Check my channel when you get a chance. Not quite dic beautiful but I'm getting there lol. Thanks and subbed.
@@SLAUGHTEREDEYES Thanks for subbing, i will check out your channel :) If you want to further push your microscope maybe the following blog will be interesting for you: bettermicroscopy.blogspot.com/2017/04/3d-image-enhancement-made-easy-part-i.html