I couldn't be me more thankful to you for these videos. Amazing. I have been studying a paper for a PhD interview and I couldn't understand a thing. But thanks to your videos, now I do. Thank you again. Keep up the good Work, Please.
Regarding the step 3 of transgenesis, the procedure through which the transgene is introduced is called TRANSFECTION. Transformation is done autonomously by micro-organisms ( as demonstrated by the Griffith experiment). Nice video and well done.
in the 3rd step of transgenesis you insert gene of intrest with vector (plasmid) , why cant we just directly inject naked gene into the male pronucleus
Transgenic insertion of a plasmid containing the mouse gene of interest may be performed to generate an overexpression model, but this has an unknown number of gene insertions. For a single-copy insertion overexpression model, we can perform PiggyBac-on-BAC transgenesis.
I couldn't be me more thankful to you for these videos. Amazing. I have been studying a paper for a PhD interview and I couldn't understand a thing. But thanks to your videos, now I do. Thank you again. Keep up the good Work, Please.
how is proteins like primase and polymerase are created/?
Regarding the step 3 of transgenesis, the procedure through which the transgene is introduced is called TRANSFECTION. Transformation is done autonomously by micro-organisms ( as demonstrated by the Griffith experiment).
Nice video and well done.
Did you test it on yourself ??
Great Explanation! Thanks for the effort!
Very well made Explainty! It's a shame your Channel and Videos don't get as much attention as they deserve.
Keep going, your videos are great.
Very Informative and brief. Can you please give details to prepare Foster mother by inducing progestron harmon. Timings, dose and other details.
plasmids occur in prokaryotes. can recombinant plasmids be inserted into mice?
Yes
it sounds like a complicated selective breeding...
in the 3rd step of transgenesis you insert gene of intrest with vector (plasmid) , why cant we just directly inject naked gene into the male pronucleus
Could i use this technique to over-express a gene already in a mouse? If not, what other methods can i use for this?
someone over-expressed my balls genes; they are HUGE
Transgenic insertion of a plasmid containing the mouse gene of interest may be performed to generate an overexpression model, but this has an unknown number of gene insertions. For a single-copy insertion overexpression model, we can perform PiggyBac-on-BAC transgenesis.
Thank you❤️😊
From Sri Lanka 🇱🇰😍
Thanku.for giving such a valuable content....👍
Thank you, for your nice explanation!
@@Cyagen please don't I will have to report you
Thank u so much for explaining and the animation
Are you volunteering?? It's just an injection 🤣🤣🤣🤣
@@zabranjenakojasko6833 IDK what u mean they rlly explained it good
Me: Okay so let's search to find the answer it's 21st century after all
The video: the exact mechanism has not been determined
Me: 👁️👄👁️
i like this explanation but the “youtuber” accent is rlly annoying lol