Your videos are a treasure! One year ago I watched your DIY autoclave video and next week I’ll be starting a job in plant genomics. On paper, I’m completely unqualified. But the knowledge I’ve amassed from your channel gave me a shot. You’re making a huge difference if a shmuck like me can get into these spaces. Keep up the phenomenal work!
When I first learned how to do this I had the biggest nerdiest smile on my face thinking about how I could use this method. I don’t have the equipment since it was the companies equipment and I wasn’t really looking because of how expensive it typically is for their methods. Though seeing your video makes me want to get back into it and buy the stuff for my own garden.
This will be extremely useful for growing cactus from seed. When we grow cactus from seed, we typically grow it under cover for weeks or months or even years, so the seed doesn’t dry out and die or get stunted. Cactus seedlings have minuscule root systems and it takes ages for them to develop. Normally we sterilize the soil in the microwave and then grow it in some kind of plastic container with a lid. I’ve had a lot of failures with this method due to mold and algae finding their way inside and either suffocating the seedling or causing damping off. Next winter I wanna abandon my caveman ways and do it the proper scientific way.
Since cactus seeds are soooo tiny, you could also use a micropipette with sterile tips to pick up the seeds in the solution and place them directly onto the media. I am planning to try that soon with some gibbaeum seeds
@@fruitofpalm4668super super long and boring basically you just watch the soil i m about to rage quit this experiment and already buy full grown cactus lol
I really enjoy your channel. I don't think that I will ever try to perform any of the operations that you do on tissue culture or plants, but it is fascinating to see how technology can improve productivity.
I have to say this is the video that woke me up to this❤. I'd seen so many tissue culture videos on FUNGI, not with plants, at least by how I was searching. My first experience with it was really at Disney World, the place known as pleasure island, had some really cool stores and they sold sequoia trees in testtubes. and of course they were adorably tiny in there and I wanted to buy one, but I was afraid I'd kill it as I didn't know sooo much at 15 years. I think you're channel is a gem and your vocal delivery has a jovial personality that brings an inner child to life within me as I watch and learn from you. May you in all facets of your life know true, blessed joy.❤❤
@Micheal-uc5qb I used to live in Orlando, Fl. If the Virgin Music Mega store is still there it's in THAT shopping spot (Pleasure Island, adults only when night falls. This was 29 years ago. I'm old now!)
Have you ever looked at using starsan for sterilizing hard surfaces? It makes like 5 gal from 1 oz concentrate and is used in brewing and restaurants a lot.
Back when I worked with mammalian cells we would give them a bit of penicillin/streptomycin to reduce the risk of contamination. It's probably poor practice due to the whole antimicrobial resistance issue we've got going on, but it allows the students to not contaminate all of their samples.
Thanks for the video! Can you give me a hint for my containers pls? I sterialize them in the microwave but as soon as it cools down they suck the lid and the container gets dents from that
I came here to learn about plant tissue culture techniques, and stayed not only for the marvellous content, but because you are the sexiest, funniest nerd I have ever seen! I used to love watching the Big Bang Theory, and I have to say, if you took the three leading ladies ie Penny, Bernadette and Amy aka Melissa Rauch, Kaley Cuoco & Mayim Bailik and somehow amalgamated them together, you would get YOU! Beauty, pazazz and brains. I know these days that you aren’t supposed to say things about peoples looks etc, but I just had to! Also, your humour is off the charts dry and funny as heck. Thank you for posting all you do. Content, 10 out of 10. You…., You destroyed the scale!
Thanks for another excellent TC video! Anyway, a question. How long roughly from initial seeds to planting seedlings in the soil? This would be the seed - explant - cultures - soil option and for sunflowers.
amazing video!! one question, 5ml of h2o2 and 45ml of water gives 0,3% of final solution, that is not very weak to sterilize? usualy I like to use 1% solution
Interesting to know how easy this process is once you have all the equipment and knowledge. Thanks for making this video to educate us. However, I don’t mind waiting a year to collect seeds from the best plants as that’s what I do every year anyway.
Great video.. thanks from the UK. This would be great for difficult to germinate seeds. I would have loved to see the cutting procedure. I was expecting you to use the baby growth point, not the leaves.
Thank you for this video. I have no interest in all the cloning and stuff, I just like your channel and the processes you employ (I'm a nerd). The seed starting though! That's new to me and exciting. It's a different way for me to experiment starting seeds for my garden. I begin the process every January, so I have time to prepare. Thank you ^_^
Great channel, fellow botanical nerd here. U are far more advanced in Tc than me. Lol I started here and ended up in fungal tissue culture but still love plant tc
Thanks for video. It is great to see you share your passion. For beginners, a Bunsen burner on a clean surface is cheap way to create a sterile working space.
-actually a cool biology/engineering/technology video that might be useful -linked products -in vid ad, followed by yt ad -good thumbnail -literally can sell the results of the video (ok maybe sunflowers are whatever) Bro is grinding
At the rate this is progressing, we'll soon get a video showing how to extract and culture your own bone marrow stem cells using kitchen utensils and a pressure cooker.
Ola, sou brasileiro e estudo para ser engenheiro agronomo. Adorei o video. Apos as folhas de girassol se tornarem calos, voce passa para um meio de cultura convencional MS igual o que usou para germinar? Ou necessita de outros hormonios para tornar os calos em plantas novamente?
It just dawned on me that your channel would literally blow up after a nuclear war..........................................................................................................................
When you do the replicating step. How long can a plant stay in there before you are forced to do something with it?? Just wondering how often work needs to be done while rotating a catalog of plants over long period of time.
Very interesting technology. Ever since I got to know Kallus cultures, I have been fascinated by the possibilities. It's also more fun than with animal cells, both ethically and technically easier!
@@mochartsmo2122 I bought 4 ft lights from Hortipower and attached them to the Home Depot shelves. Hortipower sells grow racks but they are extremely $$$
I'm doing classic gardening and it does feel caveman like in comparison to TC. What would you say would be a good application for the technique you ahowed here? I'm thinking about expensive seeds, but I'm not sure if TC would come out ahead for garden plants.
Vanilla is really easy to clone in TC. To get a specimen sized plant to produce a seed pod is another story lol. Saffron also yes, but TC won't make it cheaper because the problem is each plant yields very little. Truffles are fungus so I know nothing about them...
I’ve had this thought in my head a few days… if you add two types of the same plant like say Thai constellation and a albo monstera could you cause a hybrid in tc? Maybe if the tissues are “grafted” to each other might help get some cells to swap. Just a thought, could be a cool experiment.
That’s exactly how it works, it’s actually a breeding technique that already exists. What u described creates a chimeric cross. Although not a hybrid because the plants u described are in the same species. U could take it a step further by a another method known as “Cellular Fusion” by coating the cells with polyethylene glycol, which dissolves away the membrane, letting the cells merge, replicating natural hybridization. Nowadays u don’t need to wait till maturity.
Naturally cells will not merge unless the membrane is penetrated… if u remember in school… reproductive cells has the ability to penetrate the other team thus allowing fusion, while with vegetative cells that layer has to be removed using various ways.
@@mil0thebird possible, u can even do it without TC. Find a Thai constellation monstera stem with a bud cut off the stem just right in the spot it's growing a bud clean and straight with precise tools, try your best to make an exact half cut of the bud-junction and do the same to the pink princess. Connect the top half with the bottom half. And apply keiki paste on the bud eye. Now take good care. pruning off other potential growth points may lessen the wait although not nessasary.
The larger ones sometimes do get crushed if you stack them. If you have a laminar flow hood, you can pour the media under the hood. The small ones I use from lollicup also do not crush in the autoclave, even when stacked. Hopefully that helps!
I bought some from Phytotech when I was first getting started. Not sure if they still sell it though. Once you get the hang of it, the media is very easy to make.
Big plant been real quiet since this dropped.
😅😅😂😂❤
０：
Thanks for bringing joy to this retired plant geek!
thank you!
Your videos are a treasure! One year ago I watched your DIY autoclave video and next week I’ll be starting a job in plant genomics. On paper, I’m completely unqualified. But the knowledge I’ve amassed from your channel gave me a shot. You’re making a huge difference if a shmuck like me can get into these spaces. Keep up the phenomenal work!
When I first learned how to do this I had the biggest nerdiest smile on my face thinking about how I could use this method. I don’t have the equipment since it was the companies equipment and I wasn’t really looking because of how expensive it typically is for their methods. Though seeing your video makes me want to get back into it and buy the stuff for my own garden.
This will be extremely useful for growing cactus from seed. When we grow cactus from seed, we typically grow it under cover for weeks or months or even years, so the seed doesn’t dry out and die or get stunted. Cactus seedlings have minuscule root systems and it takes ages for them to develop. Normally we sterilize the soil in the microwave and then grow it in some kind of plastic container with a lid. I’ve had a lot of failures with this method due to mold and algae finding their way inside and either suffocating the seedling or causing damping off. Next winter I wanna abandon my caveman ways and do it the proper scientific way.
Since cactus seeds are soooo tiny, you could also use a micropipette with sterile tips to pick up the seeds in the solution and place them directly onto the media. I am planning to try that soon with some gibbaeum seeds
I just started growing cacti & various succulents from seed - great idea!
@@fruitofpalm4668super super long and boring basically you just watch the soil i m about to rage quit this experiment and already buy full grown cactus lol
Did you try it??
She makes me want to go out and get the gear and try this!!
Makes me want to visit Sicily.
I really enjoy your channel. I don't think that I will ever try to perform any of the operations that you do on tissue culture or plants, but it is fascinating to see how technology can improve productivity.
OMG OMG OMG
An affordable flow hood!!!!
ty ty ty 🎉
I have to say this is the video that woke me up to this❤. I'd seen so many tissue culture videos on FUNGI, not with plants, at least by how I was searching. My first experience with it was really at Disney World, the place known as pleasure island, had some really cool stores and they sold sequoia trees in testtubes. and of course they were adorably tiny in there and I wanted to buy one, but I was afraid I'd kill it as I didn't know sooo much at 15 years. I think you're channel is a gem and your vocal delivery has a jovial personality that brings an inner child to life within me as I watch and learn from you. May you in all facets of your life know true, blessed joy.❤❤
which disney world did you find that tell me I HAV 2 GO!
@Micheal-uc5qb I used to live in Orlando, Fl. If the Virgin Music Mega store is still there it's in THAT shopping spot (Pleasure Island, adults only when night falls. This was 29 years ago. I'm old now!)
Have you ever looked at using starsan for sterilizing hard surfaces? It makes like 5 gal from 1 oz concentrate and is used in brewing and restaurants a lot.
Any plans to do a demonstration video with the mini flow hood? 👀
Probably in my next vid! I wanted to use it for this one but I filmed most of the video before I got it
Dang that baby laminar flow hood is tempting!
I've always used polypropylene plastic bags for sterilizing tweezers and other hardware. And you can re-use them for 10's or even 100's of times.
Great video and sounds perhaps the best way to get into tissue culture before going the whole hog of trying to sterilise explants etc.
Can you germinate cactus seeds like this?
Yes 100%. But since the seeds are so small I would use a sterile micropipette to transfer them instead of forceps.
This is one of my favorite subscriptions;)
Back when I worked with mammalian cells we would give them a bit of penicillin/streptomycin to reduce the risk of contamination. It's probably poor practice due to the whole antimicrobial resistance issue we've got going on, but it allows the students to not contaminate all of their samples.
Thanks for the video! Can you give me a hint for my containers pls? I sterialize them in the microwave but as soon as it cools down they suck the lid and the container gets dents from that
I came here to learn about plant tissue culture techniques, and stayed not only for the marvellous content, but because you are the sexiest, funniest nerd I have ever seen!
I used to love watching the Big Bang Theory, and I have to say, if you took the three leading ladies ie Penny, Bernadette and Amy aka Melissa Rauch, Kaley Cuoco & Mayim Bailik and somehow amalgamated them together, you would get YOU!
Beauty, pazazz and brains.
I know these days that you aren’t supposed to say things about peoples looks etc, but I just had to! Also, your humour is off the charts dry and funny as heck.
Thank you for posting all you do.
Content, 10 out of 10.
You…., You destroyed the scale!
Thanks for another excellent TC video!
Anyway, a question. How long roughly from initial seeds to planting seedlings in the soil? This would be the seed - explant - cultures - soil option and for sunflowers.
This is my favorite channel
Love her sense of humor and enthusiasm.
What make or model is the glass bead sterilizer you're using around 9:30? I like how deep it is.
Ah the pressure cooker. The smaller humble cousin of the autoclave. Or you could call it the home autoclave I guess!
I was surprised the cotyledon leaves were used vs the first true leaves. looking forward to the longer x100 vid , thanks.
Curious, have you ever tried this method, without all of the sterilisation steps? Just to prove it's requirement?
TC does not work without sterilizing the explant
This is amazing, my line of work is nothing related, but I'm a simple man, when I see a lab and some equipment I'm all in.
This is officially my new favorite channel.
amazing video!! one question, 5ml of h2o2 and 45ml of water gives 0,3% of final solution, that is not very weak to sterilize? usualy I like to use 1% solution
I wanna see your isopods! I had no idea collecting isopods was a thing but googling exotic isopods yielded some really cool looking little guys !
I guess I'm a caveman.
Fr
I'm apparently part of your tribe then. Lol
Guys, forget them. Let's go walk in the manure.
*Picks up Spear and Leaves*
Ouga ouga !
Whole Neanderthal right now
Interesting to know how easy this process is once you have all the equipment and knowledge. Thanks for making this video to educate us.
However, I don’t mind waiting a year to collect seeds from the best plants as that’s what I do every year anyway.
Would RO water work?
When germinating the seeds, what media did you use?
I really love your humor :) I am inspired! I'm gonna try and grow some Wasabi seeds using TC
Great video.. thanks from the UK.
This would be great for difficult to germinate seeds.
I would have loved to see the cutting procedure.
I was expecting you to use the baby growth point, not the leaves.
Thank you for this video. I have no interest in all the cloning and stuff, I just like your channel and the processes you employ (I'm a nerd). The seed starting though! That's new to me and exciting. It's a different way for me to experiment starting seeds for my garden. I begin the process every January, so I have time to prepare. Thank you ^_^
Great channel, fellow botanical nerd here. U are far more advanced in Tc than me. Lol I started here and ended up in fungal tissue culture but still love plant tc
Thanks for video. It is great to see you share your passion. For beginners, a Bunsen burner on a clean surface is cheap way to create a sterile working space.
This is my Old School experience as well. Love that Bella Box...I might have to buy one.
This is alot for such cheap and common plants have you tried growing mushrooms you have the right set up for mycology
Mini Portable Laminar Flow Hood
as a one of can i use 1/ 2 PVC 4- way Elbows
and plastic or gladwrap
I also like to make 100s of plants from one plant but I take the cheaper longer route and do traditional cloning with plant cuttings.
It's all about the vibes. In a mission to prove my mechanostimulation technology can bolster plant steering processes.
Great vid... Cheers!
You appeared on my feed today. Super cool video! Subbed :D
-actually a cool biology/engineering/technology video that might be useful
-linked products
-in vid ad, followed by yt ad
-good thumbnail
-literally can sell the results of the video (ok maybe sunflowers are whatever)
Bro is grinding
broster's about to ride the algorithm to 1m subs
Amazing video! Do you ever wanna do cacti?
At the rate this is progressing, we'll soon get a video showing how to extract and culture your own bone marrow stem cells using kitchen utensils and a pressure cooker.
Don't give her ideas. Please! 😉🤣
You use the same basic things she is doing.
The funny thing is that it’s totally possible
To do exactly that
Mad scientist corner of my brain churning this over and over.
Hi! Great vids! I'm hooked. Can you explain doing TC to date palm?
Great video 🙏
Awesome, thanks for sharing. I'm sooo pleased that I found this channel!
Ola, sou brasileiro e estudo para ser engenheiro agronomo. Adorei o video. Apos as folhas de girassol se tornarem calos, voce passa para um meio de cultura convencional MS igual o que usou para germinar? Ou necessita de outros hormonios para tornar os calos em plantas novamente?
Best video I've seen on this, I like your style.
how well do these transfer to outside soil?
It just dawned on me that your channel would literally blow up after a nuclear war..........................................................................................................................
I dunno, if this is irrelevant, but i definitely need to know your skincare routine!
It GLOWS 🌟
I want to have such a flawless skin.
Subbed!
When you do the replicating step. How long can a plant stay in there before you are forced to do something with it?? Just wondering how often work needs to be done while rotating a catalog of plants over long period of time.
Very interesting technology. Ever since I got to know Kallus cultures, I have been fascinated by the possibilities.
It's also more fun than with animal cells, both ethically and technically easier!
Man I need to find someone like you wow love your energy
Hello, I would like to know, where did you get these nice culture grow racks?
The shelves? They are from Home Depot. Amazon also sells similar ones
@@plantsinjarsohh really, whats the name? i was searching for shelves with lights but i didnt find any good ones
@@mochartsmo2122 I bought 4 ft lights from Hortipower and attached them to the Home Depot shelves. Hortipower sells grow racks but they are extremely $$$
@@plantsinjarsDo you use the Hortipower Nuser 1, 2, 3, or 4? Also, would you suggest for growing microgreens?
Nurser 3. I don’t grow microgreens, but I don’t see why they wouldn’t work
It's cheaper to buy 100 seeds
Hahaha
Yes but it is less neat
I love this resource. You're a G
I loved the video, I'm going to try to do it at home
I'm curious about trying gibberellic acid to germinate old seeds.
Do you find those polypropylene pots crumple in the autoclave ? The company selling them advises against sterilising with the media in them
I'm doing classic gardening and it does feel caveman like in comparison to TC. What would you say would be a good application for the technique you ahowed here? I'm thinking about expensive seeds, but I'm not sure if TC would come out ahead for garden plants.
Have a great Month!
so knowledgeable and cute? cant be true! lol keep up the great work. I'm new to your channel but loving the videos! thank you
Would something like this work for funguses that are hard to grow like truffle? Vanilla and Saffron prices bouta plummet
Vanilla is really easy to clone in TC. To get a specimen sized plant to produce a seed pod is another story lol. Saffron also yes, but TC won't make it cheaper because the problem is each plant yields very little. Truffles are fungus so I know nothing about them...
I love that you're like...holding back laughter from your own little subtle jokes and dark humor lmao
Back in my day we just died when our crops didn't grow
This video delivered, and I was very skeptical. Thank you.
Caveman option is better...i would prefer to buy 100 seeds to grow 100 plants rather than buying all expensive equipments to grow 100 plants😅
I’ve had this thought in my head a few days… if you add two types of the same plant like say Thai constellation and a albo monstera could you cause a hybrid in tc? Maybe if the tissues are “grafted” to each other might help get some cells to swap. Just a thought, could be a cool experiment.
That’s exactly how it works, it’s actually a breeding technique that already exists. What u described creates a chimeric cross. Although not a hybrid because the plants u described are in the same species. U could take it a step further by a another method known as “Cellular Fusion” by coating the cells with polyethylene glycol, which dissolves away the membrane, letting the cells merge, replicating natural hybridization. Nowadays u don’t need to wait till maturity.
Naturally cells will not merge unless the membrane is penetrated… if u remember in school… reproductive cells has the ability to penetrate the other team thus allowing fusion, while with vegetative cells that layer has to be removed using various ways.
@@Micheal-uc5qb that’s really cool thanks for the info, I want to try it now!
@@Micheal-uc5qb now I’m dreaming of a pink variegated monstera. Thai x pink princess maybe 🤔 would that be possible?
@@mil0thebird possible, u can even do it without TC. Find a Thai constellation monstera stem with a bud cut off the stem just right in the spot it's growing a bud clean and straight with precise tools, try your best to make an exact half cut of the bud-junction and do the same to the pink princess. Connect the top half with the bottom half. And apply keiki paste on the bud eye. Now take good care. pruning off other potential growth points may lessen the wait although not nessasary.
Aluminum foil is the street method?? We used that all the time on class!!
Let me ask you a layman's question, is this method cost competitive on a production scale? (1-10hec for exemple).
I find autoclaving typically crushes polypropylene containers, do you find that not sealing them helps?
The larger ones sometimes do get crushed if you stack them. If you have a laminar flow hood, you can pour the media under the hood. The small ones I use from lollicup also do not crush in the autoclave, even when stacked. Hopefully that helps!
Does plant cell technology have issue with purchasing supplies often. They have been out of MS forever?
It will be back in stock so soon!
Dr pepper tofu flavor !! Omg !!
Nah bruh lavender honeysuckle sprite
Now i need to taste this !! Only in japan
I bought a Hanna instruments pH meter it lasted exactly one month past the warranty and they didn't offer repair service.
Whose pH meter lasts?
PH meters are ALL garbage and failure is a question of when, not if. Use PH test strips instead.
I love the fact that you are a beautiful, funny, intelligent, nerd... so cool... Love it
What in the world of gardening did I stumble upon? This is wild but amazing!
What are you studying faculties, so you start up making this beautiful masterpiece stuff?
where did you buy this still air box?
Hi could you tell me can banana peel be used as tissue culture if yes how?
Probably not but I have a banana specific video in my writing drafts
best intro
Very dumb question, how much water is in the pressure cooker when you sterilize things?
Depends on the PC, but mine is 12 cups
@@plantsinjars Is there a rule of thumb with the depth? Or is it something that would be specified in the manual? Also thanks!
Excellent 😃🎉
I subscribed to your channel 🙏🌱
water melon seeds did you nick tham to let the water in slit nick on the point as a example ( the outer skin )
I was wondering if there was a reason you chose to use solid media instead of the martini shaker method?
@plantsinjars can you do a video about engineering glow in the dark house plants?
JUST FOUND YOUR CHANNEL AND I AM GLAD
I knew a guy that had 27 kids, I now understand the power of seed.
amazing.. new thing learned ❤
Your teeth look like diamonds wtf
Is there a place I can I buy a premixed medium?
I bought some from Phytotech when I was first getting started. Not sure if they still sell it though. Once you get the hang of it, the media is very easy to make.
Appreciate you!
She’s so pretty wow
Great video 👍
you used the cotyledons not the leaves
This
You should try Madagascar Hissing Cockroaches if you love isopods.
I can see the applicability of this technology really only to rare or exotic seeds.