AK LECTURES ! You are the best lecturer on youtube. To be honest , You are even better than my prof. in my school. I wish I could only stay home, eat, sleep, and watch your video for my education haha JK!. I know I am so lazy for not going to school someday, if that such a case, you are my savior.
I've been watching your videos recently, it is clear and easy to understand eventhough I am not a native english speaker. thank you for the videos, and it really helped me through the mid term test!
oh my goodness! I am really impressed by the quality of your teaching skills! Thank you loads; You helped me to finally understand what my biochemistry prof can't bring across in lectures at uni! You saved my good mark
wow thanks! you are one of the only people to have heard those pieces.. not many people know about them. funny you listened. thank you so much! your work has helped me tremendously and i am so grateful.
yah i did. i'm surprised you like it.. that's so nice. i have a few more pieces, but haven't recorded them because i've been locked up studying for the mcat. wouldn't have been able to make it this far without you actually. so thank you again. it's really nice to know someone likes my music.
In this lecture, when you are explaining the technique of Isoelectric Focusing, you mention that a protein with a positive net charge would move towards a higher pH. This confuses me because I have understood, and correct me if I am wrong, that if the protein has a positive net charge then it has a "surplus" of H+ atoms, therefore, I would assume that the protein is acidic. I appreciate any explanation or clarification! Thanks a lot!
Sorry, one question. For Aspartate (acidic side chain). Why do we only average alpha-COOH and side chain? If the pH=3.6, then, alpha-COOH will be negative (pH>pKa) , side chain will be positive (pH
Why would IEF gel not denature protein? Since by going to their pI, you're messing with protein's charge (which in turn mess with their ionic & H-bond).
These are really good lectures. Just one minor tip, maybe try to move less. It's distracting from the lecture. The rest of it is amazing. Helped me really well for my test.
So theoretically if you placed a single unitary amino acid (the one you explained in step 1 where the pI would be 5.55) anywhere on the gel, the amino acid will migrate to its isoelectric point by the nature of the isoelectric focusing process - is this correct?
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Congratulations on your incredible knowledge, fluency and dedication to the task of disseminating the biochemistry.
AK LECTURES ! You are the best lecturer on youtube. To be honest , You are even better than my prof. in my school. I wish I could only stay home, eat, sleep, and watch your video for my education haha JK!. I know I am so lazy for not going to school someday, if that such a case, you are my savior.
You can do just that now! It's quarantine
This comment aged well, lmao. Now we're all doing that!
There's no one in the world who disagree to that 🔥🔥🔥
I've been watching your videos recently, it is clear and easy to understand eventhough I am not a native english speaker. thank you for the videos, and it really helped me through the mid term test!
The interwebz respects people like you, who actually manage to make things clear in a clear way. Go Humanity!
Thanks
oh my goodness! I am really impressed by the quality of your teaching skills! Thank you loads; You helped me to finally understand what my biochemistry prof can't bring across in lectures at uni! You saved my good mark
you're such an amazing guy. thank you for all your work!
thanks Emily! I like the piano pieces! talented :)
wow thanks! you are one of the only people to have heard those pieces.. not many people know about them. funny you listened. thank you so much! your work has helped me tremendously and i am so grateful.
Well it was a pleasure to listen to, especially "Noch". Something about it just made it so relaxing and peaceful. Did you compose it?
yah i did. i'm surprised you like it.. that's so nice. i have a few more pieces, but haven't recorded them because i've been locked up studying for the mcat. wouldn't have been able to make it this far without you actually. so thank you again. it's really nice to know someone likes my music.
The best explanation EVER! Thank you :)
You are so amazing. Thank you so much.I love you.
I love this. Thank you for taking time for doing this!
Finally I do understand the Ip. Thank you very much for amazing explanation.
I don’t usually comment on TH-cam videos, but thank you so much- this was really helpful!
Thank you so much! I love your videos and the way you teach:)
Great video, thanks a lot. You are a great teacher!
In this lecture, when you are explaining the technique of Isoelectric Focusing, you mention that a protein with a positive net charge would move towards a higher pH. This confuses me because I have understood, and correct me if I am wrong, that if the protein has a positive net charge then it has a "surplus" of H+ atoms, therefore, I would assume that the protein is acidic. I appreciate any explanation or clarification! Thanks a lot!
OMG you're the best lecturer!!!! Thank you so much for making these videos!
Great Explanation 👍👍 AK
YOU ARE THE BEST!!!! OMG I CANNOT THANK YOU ENOUGH.
Seriously...why are u less than 1M subs? Ur a genius my friend.
For case #2, best NOT to memorize but rather understand. Fully protonate at first (i.e., low pH) and then work your way up.
If group 4 only contains Cysteine and Tyrosine, where do Serine, Threonine, Aspargine and Glutamine belong? In which of the 4 groups? Thanks! :)
I’m teaching myself biochem bc my profesor sucks. Thanks for your lectures this is helping me too ;)
For the tyrosine, isn't the charge on the hydroxy group supposed to be OH not O- since the pka is higher than the pH?
I understand PI separation methods thank u for best explanation !!!!
thank you so much sir for explaining such complex topics so easily...
Excellence as always!!!! Much Appreciation !
Awesome explanation 😍🤩
thank you for existing... really
Amazing teaching skills keep it up thanks👍👍👍
Sorry, one question. For Aspartate (acidic side chain). Why do we only average alpha-COOH and side chain? If the pH=3.6, then, alpha-COOH will be negative (pH>pKa) , side chain will be positive (pH
That's what I've been wondering too...
The side chain won’t be positive but neutral. COOH is neutral when acidic and negative when basic.
Why would IEF gel not denature protein?
Since by going to their pI, you're messing with protein's charge (which in turn mess with their ionic & H-bond).
This is very helpful! I wish you were my biochemistry professor 😩😩😩😩😩
I wish you were my mentor!
Thank you so much!
Just perfect, thank you
awesome awesome job. thank you so much for these they're so helpful!
These are really good lectures. Just one minor tip, maybe try to move less. It's distracting from the lecture. The rest of it is amazing. Helped me really well for my test.
test in two hours. lets do this
how did you do?
Is the negative end of the voltage source always connected to the basic end of the gel? Thank you :)
great video, very helpful!
Very well explained - thank you!
+Maria Xhako Thanks again !
This is really good!
you're the best ever
Thanks a lot
best explanation... thank u..
So theoretically if you placed a single unitary amino acid (the one you explained in step 1 where the pI would be 5.55) anywhere on the gel, the amino acid will migrate to its isoelectric point by the nature of the isoelectric focusing process - is this correct?
thank u
@ak lectures please help me, how can I find diffusion coefficient of each peptide/amino acid? which formula should I need to use?
Thank you!
the best lecture
please expand the biochem series ....This is about where we're at for this current semester.
yes, thats what I am current working on now. my website will have the complete biochem playlist so you can follow it there! good luck! :)
How to separate two proteins with same isoelectric point?
Correct me if I'm wrong...but I believe your tyrosine structure is missing a carbon.
thanks
i love this video... :)
Which book u follow sir??
Amazing, I love you, no homo.
12:18
nyc :) (y)
And what that means is... XD