In electric fields, carrier ampholytes partition into smooth pH gradients that increase linearly from the anode to the cathode. The slope of a pH gradient is determined by the pH interval covered by the carrier ampholyte mixture and the distance between the electrodes. After the pH gradient is created, no further separation can happen in the electric field, which maintains the pH gradient. Then you apply your sample and now only only your protein sample will move according to it's pI value.
Awesome ,I have a test on Electrophoretic techniques tomorrow, Thank you for clearing the concept
Glad you liked it. Do share
You are awesome ... Thanks to explaning in such a easy manner... Thanks a lot❤️
😊😊😊
Superb
Thankyou 😊
great video and explanation!
Glad you liked it. Do share
Nice ❤️❤️
Thankyou
Nice explanation thank you
😊😊😊
Clear, lucid explanation👍🏻
Thankyou dear....do share
nicely explained. thanks ma'am.
Glad you liked it. Do share
Well explained
Thankyou
Very helpful, thank you!
Happy to help ☺️
Very helpful 🙃thanks🙃
Happy to help 😊😊😊
Clearly understood..Many thanks!!
😊😊😊
Thank you so much for making this video
Glad you liked it. Do share
Love u mam for this wonderful explanation
Thanks 😊😊😊
Thank you. High informative
Glad you liked it. Do share
thank you so much..it was highly informative
Glad you liked it. Do share
Just amazing...
Happy to help 😊😊😊. Stay safe
It was very helpful
Thanks 😊
Great explanation thank you.
Welcome 😄 do share 😇
Very much helpful ❤️
Glad you think so!
what does the music ha to do with the PI? i dont understand explain plz
Music ?
Very helpful 👍🏽
Thank-you. Do share 😇
Thank you ❤️
Welcome 😊😄
Thank you for your video :)
😊😊
why does the positive charge decrease on the molecule as it moves towards the cathode?
The charge is changing due to the pH gradient we created ( due to change in pH)
Thank you!
Glad you liked it. Do share
Thanks 🙏
😊😊😊
Thank uuu so much ma'am 😊😊
Happy to help 😊😊😊
Tanq mam for such a vedio
Glad you liked it. Do share
Mam how can we know that a single band at PI has a single band or multiple band ?
U mean how to know there are more than one protein in one band? Well for that u can do other techniques like western blot
Okay mam 😊thnku so much
Happy to help. Stay safe 😊
Awesome
Happy to help 😊😊😊
Thank u mam 😊
Happy to help 😊😊😊
How pH Gradient over a gel is maintained at different level ?
In electric fields, carrier ampholytes partition into smooth pH gradients that increase linearly from the anode to the cathode. The slope of a pH gradient is determined by the pH interval covered by the carrier ampholyte mixture and the distance between the electrodes.
After the pH gradient is created, no further separation can happen in the electric field, which maintains the pH gradient. Then you apply your sample and now only only your protein sample will move according to it's pI value.
Thank you
thank you soo much
You're welcome!
awesome
Happy to help 😊
Refer link for updated video without music: th-cam.com/video/iGlPtSx-Fe0/w-d-xo.html
Thanks.... :)
Glad you liked it. Do share
Thanks a tonnes😃
Glad you liked it. Do share
thnk yu
Welcome
Computation method
🥰🥰🥰🥰🥰🥰
thankyou
Video was so informative thnk you so much..... music was soo irritating no need for any king of dumb music 🙄🙄🙄
yes got it. thaNKYOU
Sis please remove background music
Yes I understand it is annoying. I kept it down for future videos. Will reupload these videos without the music asap
Reading wilson walker..still wrong reading
What is it that you did not get?
Not satisfied 😐
What was missing ?
Chanchal tyagi
OMG. You started this, and yet BINOD got famous. sorry bro
Music is so distracting ! Can't watch at all
i reuploaded this video without the music. New video link is pinned on the comment section. Sorry.
:)
Why sad
No no...posted by mistake....actually you saved me :)