The Microscope Upgrades We've Made Along The Way | Compilation
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- เผยแพร่เมื่อ 31 ก.ค. 2022
- Go to microcosmos.store before August 8th to get 10% off of almost everything in the store and to pick up a limited edition Dark Mode Hydra T-Shirt!
This channel wouldn’t be what it is if it weren’t for one very key invention: the microscope. Everything we see, we see with the aid of light and lenses, expertly deployed by our master of microscopes, James. And if you’ve been on this journey from the beginning, or if you’ve ever gone back to revisit our earlier videos, you may have noticed that things have changed a bit around here.
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SOURCES:
micro.magnet.fsu.edu/primer/a...
micro.magnet.fsu.edu/primer/a...
www.geog.ucl.ac.uk/resources/...
micro.magnet.fsu.edu/primer/t...
micro.magnet.fsu.edu/primer/j...
www.microscopyu.com/technique...
micro.magnet.fsu.edu/primer/t...
www.microscopyu.com/technique...
www.microscopyu.com/technique...
www.microscopyu.com/tutorials...
www.microscopemaster.com/diff...
www.microscopyu.com/technique...
www.microscopyu.com/microscop...
www.olympus-lifescience.com/e...
www.microscopyu.com/tutorials...
www.microscopyu.com/microscop... - วิทยาศาสตร์และเทคโนโลยี
Go to microcosmos.store before August 8th to get 10% off of almost everything in the store and to pick up a limited edition Dark Mode Hydra T-Shirt!
Second :)
The store looks so wonderful. Visually speaking. Hope I will get a microscope from there soon. By the way it will be really great if we have an interview with James where Hank will be the host. Most probably I am not the only one who desires it sooo badly!! Greetings to Journey to the Microcosmos team.
Helo
God is Great ! Life is GOOD ! :-)
the channel starts slowly becoming more about james then about the microbes lol
It’s 2:27 am here in Aus. And this is exactly what I need Mini lads with James n hank
🦠mini lad
1:49 am here, same. Chillin with the micro bois.
3:33 here
Same 2w later hahaha as soon as i read the comment
nice
It would be great if James could do a video on his workflow from his technique for wet slide preparation to video recording.
When I first saw the channel I wondered about the instruments used. This is a fantastic explanation of the technology itself. Thank you James & team.
Me watching this video, trying to rekindle my love for using my for me expensive enough little microscope (400.-) and seeing his type of microscope model going up for 35.000.- from Zeiss :-o
God it is so hard not to compare yourself with this kind of stuff.
It’s always fascinating to watch these videos! Teaching urinalysis, my students were excited to see what was in it. There were so many things they identified. Crystal shapes was always the most fun. It wasn’t just liquid anymore.
MLS here - my favorite crystals are calcium oxalate monohydrate form. I discovered on my own that they polarize. They look similar in size and shape to RBCs, which do not polarize. also I love seeing motile bacteria =)
People gaze down at the tiny critters and are stunned by their beauty... Then they look up to the Stars and Galaxies, and remember we too, are tiny critters ! :-D
@@SeaJay_Oceans that's not what determines something being small
@@AR15andGOD said the teeny tiny bug stuck to an itzy bitzy mud ball planet floating around an insignificant yellow star...
;-)
@@AR15andGOD Actually, I feel that comparison between objects and/or levels in systems is exactly how one goes about determining something is "small". Everything is relative to everything else
This was a very nice and visually interesting journey of your group's progress in photographing the aquatic micro-world. The new Zeiss microscope was money well spent for your teaching abilities. However, tell James that the excellent picture of his rotifer in 36:03 to 36:30 is not a Keratella. It is a closely related loricated rotifer called Lepadella; probably Lepadella ovalis.
Thank you for your videos. I lost my uncle today and your videos have given me a calm and a piece in this moment in this day that I could explain but I just want to get back to watching micro. so just thank you. My uncle would have liked you too.
Yknow I've always wanted to see a microscope that can somehow do like a lightfield in realtime so the entire depth can be in focus simultaneously because the most annoying thing about microscopy is the incredibly shallow DOF and constantly having to adjust focus.
There is a software based digital camera that can do exactly as you say - I forget the name of it, but it basically gives you 100% DOF by processing all of the captured light and focusing all of it (computationally) so that the final image is ALL crystal clear. Not exactly 'real time' but very close, a few miliseconds delay as Billions of circuits transform data form one form to another, and produce for you, the detailed image for your analog eyes!
I totally agree. When I got into really up close macro photography I learned about just how shallow depths of feilds can get for different lenses or how your lense works with your sensor of your camera. So ya it would be great to see like a triple or quad stacked layer machine that puts all the depths of feilds into one continuous image.
@@SeaJay_Oceans do you mean LYTRO ? yeah light field cameras are AWESOME 😍😍😍
@@carbonium1264 Ya, i think that's it - a digital camera that captures ALL the light and the focus is all done in software...
@@benmcreynolds8581 Yeah. I love macro photography, It’s so cool to see ordinary creatures in higher resolution than your own eye can resolve.
“If you have ever taken an optics class… you will remember how terrible that was” - as a physics major I feel this personally
I have never learned more about light scientifically, all at once and so simply, than here. And I’ve studied photons, waves, energy quite a bit.
Microbes can make selfies now ;)
15:42 - I didn't know I've been occasionally looking at microscopic DIC pics. The things you learn watching this channel!
Hey! Optics class wasn't THAT bad! (Love the descriptions of the methods while comparing the video footage - really nifty!)
Very well presented. When the micro-world was introduced in middle school, that was when I knew I was going to be a scientist. I hope this inspires today's students...👍
Hank you're the next david attenborough. phenomenal VO work, amazing documentary here
So much information……..so little time. Thank you for the explanations of the different filters and their effects on light.
This channel is absolutely amazing to me, thankyou:) I love watching the episodes or just projecting the footage while making music to put me in a good space. Are there are any more collections of just footage out there? I've looked but can't really find any
I just gave a compilation video a thumbs up, and that is something I never do. But this one is the rare examples of where a compilation video was absolutely the right thing to do (short of filming an entire new video that encompasses the entire combination of scripts).
The job I work at is very sad, and not at all what I want to be doing, but seeing this video in my notifications pushed me through today.
What's your job?
Totally awesome.... as an amateur, this was a helpful overview.
I love your content! I'm a grad student currently, and we spinning disk confocal microscopy to visualize fluorophore tagged proteins in living neurons, so we can see how and where they traffic in the cell.
I’m a lab intern and my favorite thing is the confocal lol, it feels like flying a spaceship. We do retinas!
@@franzferdinand1782 That's really awesome, and same feeling as well!
A very important video. Cheers for all the information you've provided within it. 🍻
Your videos bring me joy 🥹 thank you.
people should watch to learn more on youtube. Your effort and also teaching style increadible. i found this channel while looking something to learn and you deserve the best. thanks for all effort.
Greatest video i've seen this year. THANK YOU!
I enjoyed your video and your presentation of how the optics involved can enhance what can be seen through a microscope. One thing that I might have given a bit more attention to is how limited the depth of field is under a microscope’s high magnification. It tends to create the impression that microorganisms are relatively flat, when in fact we are seeing a cross section of a largely transparent object. We learn to compensate for this by shifting the focus up an down a tiny bit, essentially trying to create a an imaginary “stacked image” in our mind’s eye of the organisms more 3-dimensional shape. Very few of will ever get the chance to use the most sophisticated optics, such as DIC, to observe that 3-dimensionality more directly. But, for most of the history of the study of microorganisms, a bright field microscope and an artistic talent provided the content for our textbooks.
Love that T-shirt print. I had to buy one. First thing I've liked so much to buy from a TH-camr store
Wow, thanks for sharing... this is really interesting and inspiring, science is cool
Absolutely amazing. Wow! Super cool. 👍🏾😁
Wow! 😍 So wonderful to see Nature's little surprises w/o "staining"! 😆 Your lighting brings out the eye candy of delights! 😍Thank you!! 💋
James is killin it!
Congratulations on the new microscope :D
Though lengthy this was wonderful. I finally became a Patron of your channel. I will be looking forward to the improvements of James's equipment. I'm glad the other producers are also evolving into this hobby.
I was impressed that these productions avoid employing stains that might alter the behavior of this amazing world.
Q: what does a microbe actually look like?
A: Laurence Fishburne: do you think that's air your breathing?
gee thanks hank.
כל הכבוד על הפרויקט. תודה רבה.
I just ordered yesterday. Dad burn it! Got the whole setup.
what great footage, i thought this one was edited abnormally well too.
Thanks for the upgrade, guys. 😁
Wow! Fascinating!
I found my first tardigrade 2 days ago using a pre-1880 Brass drum microscope.sometimes less is more!
That's amazing. Great jobs!! 😎👍
what a wonderful journey❤😊
This is a magical episode ❤
Amazing footage!
4:50 I didn't appreciate that rotifer flipping me off
Is there a way to capture an image with multiple processes to get a compound image, similar to how telescopes can combine IR, visible light, etc., to resolve a more 'broad' image?
I remember back when I didn't know it was Hank doing the voice over, he sounded like he didn't want to scare away the microorganisms. Good times.
Thanks so much ! Master
It's all about perspective. Microscopes look down onto/into the microbial world, a real "birds-eye view". I think about us humans being viewed just from above. It's not until you come down to our level and face us, that you get a true understanding of what we look like. I can't wait until someone invents a Microscope whose optics peer directly at microbial life on their level... something akin to a side-microscope. Until then, we can only imagine.
Are you ever going to go to superresolution or get down to the molecular scale? What molecules are involved and what are they doing? PSI, PSIi, antenna proteins for instance. Yes the videos are awesome and beautiful but getting into the Why? Of what is giving rise to the colored structures could really inspire a lot of the viewers to pursue the deep molecular science behind the images. Maybe bring a structural biologist into the mix? Love the footage!!
We love u James
Love the comment, 'If you've ever taken an Optics course - you will remember how terrible that was!'. - Oh yes - and some more! The problem was that first time around, it all just didn't make any sense. For me, it took years, gradually refining my knowledge until finally I came to appreciate just how incredible the whole subject is - and the maths is mind-blowing. Sadly, as a Physics student, I never got to see images like these, but I did eventually work with neurons and would so love to see them via these techniques.
WAW...AMAZING VIDEO ( ONE MORE TO THE AMAZING VIDEOS COLLECTION :)
THANK YOU FOR EXPLAINING ABOUT THE MICROSCOPE WORKING TECHNIC :)
AND IM GLAD FOR THE UPGRADES :)
THANK YOU FROM ISRAEL :)
is there somewhere we can find all your setup including cameras, objectives, everything that you use for your videos
ca i suggest samples from aquaculture industry such as shrimp culture (vannamei). more of the concerns are protozoa, vibrios and viruses,
Thank you!
What is the model of the phase condenser? What is the model of the microscope? Etc etc? We need detailed information please. Thanks
You can buy all the equipment you like, it ain't nuthin til James points it at sumthin.
Rather impressive I must admit
What camera do you use for dark field? I have a camera that works good in bright field but in dark field it gets real bad motion blur.
You had me wanting to touch the computer screen during the DIM section.
Amazingly clear images! Do you have any tips on how to keep your optics free of dust?
I built a cat tower over mine!
-James
I would love to see what some parasites (I.e. Giardiasis) would look like using the different microscope lighting techniques
What's a good starter microscope.
Well... Time to make some popcorn, I guess.
Here's a random question: if I were shrunk down to the size of a bacteria, how difficult would it be to punch through the membrane of a bacteria?
Aaaaaa yes this is the sleep I needed
Excellent video sir.first time could see my body. Thanks to scientists, they are my elders
Think about war, the war is because very poor understood of life. Let them understand life through microscope. God bless you and all scientist and and viewers. Thanks lot. Thanks.
We need to go upgrade further
Which camera are you guys using on the new scope?
Amazing quality
I had no clue that Hank hosted this show. I love Hank!
This is amazing. Is it possible to see bacterial phages? I saw a video going into the study of all sorts of types of phages and the possible uses of them if studied correctly. The scientists would go around and collect water samples in weird areas to find different phages that specialized in different things. Is that a possibility for you guys to do ever? Just curious?
They're microscopic even to a microscope. Some of them are only 200 atoms wide.
This is so much more interesting than big creatures
M.O.M Loves DIC....you said it, not me.
😁🤘🏻🙏💜
thank you
Cool video!
Your merch store definitely needs to sell cute little tardigrade plushies those would sell like crazy I mean I'm broke but someone would buy them
Can you check out whats happening in blood while still in the body?
I found my 60x objective on my cheaper microscope to be almost unusable even with oil. It was just close to the slide. Yet here you are using 100x, and getting great images. Do you use special extra thin slides to enable this?
i want one, please, produce more :)
Speechless.
If they make such a thing: Real time `focus stacking` for microscopy. It'd be pretty slick.
There is a software based digital camera that can do exactly as you say - I forget the name of it, but it basically gives you 100% DOF by processing all of the captured light and focusing all of it (computationally) so that the final image is ALL crystal clear. Not exactly 'real time' but very close, a few miliseconds delay as Billions of circuits transform data form one form to another, and produce for you, the detailed image for your analog eyes!
I am become Microscopy the revealer of Micro worlds
Tardigrade is cute regardless of the technique used
This review highlights the facts of how our senses work. The microscopes extend the human sense of light images upon the compound receptors we call the eye.
I am a retired psychiatrist (MD), who has been fascinated by the human as a machine as well as a spiritual being. How a human learns is just one of the many aspects that has kept my attention. All learning is done by comparison. The human does not see what exactly is. The human brain builds upon comparisons. The goal is to get a representation as close to reality as possible. Reality is that which exists beyond one's receptors (senses). Often a mistake can be made in interpretation of stimulus; this is called an illusion.
So with the microscope and the different techniques, one can get different views. This information has to be integrated. Integration is what the human mind uses to understand the environment. The closer to reality is the goal.
could you guys start uploading in HDR that would look stunning
What microscope is this pls
Beautiful
I just recalled that each and every cell of our bodies is off similar (or more?) complexity to one of these complex single-called organisms. Mind-boggling.
How about photographing in shorter wavelengths, such as far UV, and translating the image into colors we can see, like astronomers do. Is that done?
How about simultaneously recording data at a variety of magnifications, at a very high sampling rate, and composing a database that can be explored in VR to visit and revisit different aspects of the organism?
You can do higher res imaging with UV vs visible light but it is challenging (even aside from obvious safety issues) because most glasses absorb UV. You basically need every lens and bit of glass in your system to be made of specialized materials.
Early to the Microcosmos, let’s gooooo
Have you ever conciderd buying a sem microscope? For even higher zoom
can't till james gets his hands on a electron microscope
Can I take any old college level microscope and make it Darkfield and Polarized? I would love to upgrade my old college microscope and look at a dog fecal sample to view hookworm, roundworm, and whipworms under that spectacular pop of the glow!
Is computed tomography ever used in microscopy? It seems like we’re missing so much because we’re only seeing things in 2D. What if we could project a hologram of the thing we were viewing out into the air in front of us and walk around it and inspect it?
Is spectroscopy ever used in microscopy? Might not be possible to learn a lot more about an organism by looking at the spectrum of light emitted by each part of the organism when it is illuminated?
You guys really ought to do a collaboration with "Matt Powers - The Permaculture Student" - He is researching soil based microbes and fungi in an attempt to improve agriculture and gardening practices. He covers a lot of microbes and I think your channels could really benefit from working together.
Second this !
That's awesome. People who believe they can change the world and do things like that often can. I want to discover something cool too, but truthfully only as a get rich scheme. And I think that money only mindset prevents yourself from devoting, or even starting, down the path of personal research into hobbies that interest you. A lesson for myself to not only think of things for money, because it might stop me from using passion to find something that I can then sell and make lots of money 💸💰 haha
Really? I did not see that Powers stuff even comes close.
It blows my mind... Every time you are showing something tiny... there is something tinier swimming around it. Like JW in reverse.
Yesss