Sample Preparation by Wet Digestion Method for the Analysis of Heavy Metals & Minerals Using AAS
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- เผยแพร่เมื่อ 3 ก.ค. 2024
- Sample Preparation by Wet Digestion Method for the Analysis of Heavy Metals & Minerals (AAS)
Wet digestion is very popular and widely used sample preparation method for analysis of heavy metals and minerals using Atomic Absorption Spectrometer. This video represents a details procedure for the sample preparation of heavy metals & minerals analysis by Wet Digestion method. __________________________________________________________________________________________
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Video Links:
Determination of Ash: • Determination of Ash C... Standard Preparation: • Standard Solution Prep... Fe Analysis: To be uploaded __________________________________________________________________________________________
Related Tag:
#AAS
#Wetdigestion
#Samplepreparation
#Heavymetals
#Minerals
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Very intersting video, MicroChem team;
Thanks a lot
Thank you. Stay with us.
GREAT AND SMART WORK AND VIDEO
Thank you
Thanks!! This is really helpful.
Thank you
excellent
Thank you
Please are glass beads compulsory? Also, what alternatives can we use if it's not available. Thank you so much for the insightful video. Great job!!!
Good video MicroChem's Experiments, one question though where is the "Fe Analysis: To be uploaded" video? I can't find it in your channel.
Coming soon!
dear MicroChem's Experiments team: very well demonstrated.
Can we use the hot plate instead of a digester for the digestion of the dry microbial sample? can this method be incorporated for liquid samples?
Yes. You can use Hot Plate to digest your dry microbial sample. This method is also applicable for liquid sample.
Can we use this for finding calcium concentration for a plant sample?
Can v use the same method for the analysis of minerals in liquid samples using ICP OES
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Interesting video, MicroChem
1. Do you have reference method for the procedure? Perhaps by ASTM or the likes? Would appreciate if you can mention this in description.
2. Suppose the sample is a representative of larger material, is there any requirements for the sample? Particle size? Homogeneity?
3. I noticed there are two oxidizing agents used i.e nitric acid and perchloric acid. Is there any reason why? Could we make do with only nitric acid? Would appreciate if you could explain the particular reaction between each chemicals with the element of interest (maybe As or Pb?)
Anyway, looking forward for the Fe by AAS video.
Answer:
1. We developed this method at our lab by combining several methods. So no official reference method for this.
2.Sample should be crushed and blended and homogeneous portion of sample should be taken for the test.
3. Perchloric acid helps to digest sample properly. So you must use Nitric acid along with the perchloric acid to digest your sample properly.
*We will make video for Fe analysis by AAS later. Stay with us.
Hello. I have 3 questions. I hope you guys respond to me.
1. Is there any difference in procedure when we use this wet digestion method for heavy metals of fish flesh samples?
2. Incase, how will we dry the fish samples? How many grams we will be needing?
3. Is this wet digestion can detect various metals after AAS? Or is this method only specific with limited metals?
Thank you so much!
Thankz a lot Mam.
But please upload the procedure for creating method file for AAS thermoscientific
We are working on it. Please wait for few more days. Thank you.
Thank you for the explanatory video. When you left the flasks in fume hood to digest the sample in ambient temperature for 24hr do you also left the fume hood power on?
We don't practically but it should be kept the power on
@@MicroChemsExperiments Thank you for the information. I appreciate that.
@@yacobtesfamariam6400 you are most welcome Sir.
Thanks for the video. Can I keep the simple after digestion for 15 days before analysis with AAS?
You can keep it in the refrigerator at 4 degree Celsius
Nice video. Do you have any reference paper describing that procedure?
Sorry. This method is developed at our lab. No reference is available.
Which standard range (like 1 ppm, 5ppm, 10 ppm) should be calibrate for higher concentration of element of choice???
For instance 1,00,000 ppm.
Prepare calibration curve using 1ppm, 2ppm & 4ppm....not more than that. Dilute the sample to suitable dilution and analyze.
Will there be any elemental loss in fumes while heating??
Phosphorus particularly??
Nop
Heavy Metal content in food sample can be analysed by both way i.e dry ashing or wet digestion.
My doubt is which way is preferable and why?
Please give insight on this issue madam🙏
Wet digestion is the best as it has low possiblity of metal loss.
can i use a heavy metal testing kit in analyzing the sample?
Thats a different thing. You can analyse using the testing kit. But analysis using AAS provides more accurate test results.
can I use this method for the determination of calcium and magnesium both in soil and plant samples.....waiting for your kind reply....
Surely you can analyze your sample for any kind of minerals by following this method.
Thanks for the video.
How we can do the AAS test on silica and kaolinite sample? what would be the solution for dissolving them, and exactly what proportion? like this?
Follow this sample preparation strictly.
@@MicroChemsExperiments Exactly same procedure and same material?
@@saharkf3158 Yes
Good morning. I did a wet digestion using this procedure in order to dose aluminium by AAS. In the end I obtained a clear sample after filtration and dilution with pure water but after 24 hours, the sample becomes cloudy (white). I don't know the cause. Is it normal? I have never do or see wet digestion
We have mever seen like this. Repeat the test avoiding any sort of chemical contamination
Should I keep the flask for digestion in ambient temperature for 24 hrs or 12 hrs
12hour is enough
Can this same sample prepared by wet digestion method be used for flame photometry instead of aas?
Yes. You can use this prepared sample in Flame Photometry.
What about analyzing the results after the measurement on the atomic absorber .... Is there a video????. ... Thank you
We didn't prepare this prepared sample analysis video but we have other video regarding the analysis using AAS. Link: th-cam.com/video/YZ3XeXTFH1w/w-d-xo.html
For diacid, instead of perchloric we can use HCL for FE, Zn analysis. Plz reply
No
Can i apply this digestion method for the determination of heavy metals in honey by atomic absorption spectroscopy
Yes. You can use.
can i digest the food sample with the same method by using heating mantle ?
Meenakshi Trilokia, yes. You can digest on heating mantle. But you should be careful to avoid dryness of some part of the bottom surface of a conical flask or beaker. That may lead to sample loss.
Round bottom flask using is more desirable for this purpose.
Thanks for sharing, is it suitable for all sample kind ? Végétale Biomass and soil ??
Yes
can we use this method for lechate digestion instead of sample used in video ?
Yes.
Good evening. I have a tannery effluent (liquid) that I want to digest for Cr(VI) by the diphenyl carbazide method. What is the appropriate method.
Thaks
You can use this method for Cr (VI)
Why should the digestive flask be left for 24hrs? is it because we need to wait for the reaction (acids and sample) to fully happen?
Yes.
Why do we use conc acid for digestion
To digest sample completely
Can I use this method for platinum and precious group metals analysis without fire assay?
Yes. You can use
kindly provide titrametric method for the determination of selenium in sulphuric acid
I am confused about the avaibility of selenium determination titrametric method. If you have any reference known, please let us know.
Please share the sample preparation method for Organic fertilizer by ICP OES.
Noted
Dear MicroChem's Experiment team, I have a question and need help: I have weighed 1.0153g of a sample and digested with 20 ml of acid mixture, then after digestion, distilled water water added to make up the volume to 250 ml. Then 25 ml of the sample solution was taken and titrated against 0.01 N EDTA and 0.2 ml of EDTA was used to get the end point, to calculate the Calcium content and the formula I should use
This method is applicable for the mineral analysis using AAS.
Thanks for this video. I have a question. Can I digest then stock the simple before analysis with AAS for 15 days at 4°?
Can I use same procedure for leaves, stems and roots of a plant
Yes
Hello......Can you help me choose the best method of digestion.....dry or wet digestion...and what is the best weight for food samples?
Go for the wet digestion method
What about using aqua regia for sample digestion
No need to use here
can i use this procedure for greasy sample?
Not sure. But you can try
We can use both triacid Or diacid for this analysis right.
Diacid
Can I use this method to prepare my sample for ICP-MS? Please reply. Thank you @ MicroChem's Experiments team
Yes. You can
How will we use this method for spices
Same method
Can i use this method for fresh samples of milk for mineral analysis??
Yes, you can use for minerals analysis of fresh milk samples.
Can I use this method for detection of heavy metals and minerals in waste water?
Yes. You can use for waste water.
2:1 is a volumic fraction of acids?
Yes.
What is difference between partial and complete digestion??
Take help from Google
Can I use this procedure for feather digestion?
Yes. You can
Hi, can share the method for Arsenic and mercury digestion in food samples? Is this procedure applicable for Arsenic and mercury as well?
Yes. Same procedure should be followed
How will it be analyzed in AAS? i mean Residues of Filter papers will be analyzed or the solution present in a flask will? Kindly reply.
Solution present in the flask will be analysed
Can i use this method for fish muscle samples?
Shahed Mahmud, yes. You can use.
Can we use this method for preparing soil sample ( sediment)...??
You can
Can I use this method in the determination of heavy metals in palm oil?
Yes. You can.
Kindly tell procedure of texture analysis using texture analyzer.and color using colorimeter Of cake sample.
Noted. Stay with us.
Can I use this for plant samples?
Yes. you can use this method for plant sample.
For filter we can use whattman no 1 filter paper? Plz reply
Yes. You can
Why the flask must be soaked for 12 hours before used?
To remove any sort of dirt, impurities & contaminants
Very well explained 👍
I have a Question that why we prepare blank??
Blank is prepared to minimize the reagent reading.
Blank is prepared to minimize the
@@MicroChemsExperimentscan you elaborate the minimize the reagent reading?
Is calorimeter used for iron analysis in sample like seed ? Plz reply
Use AAS for best result
Can I use this method to extract gold from rock samples??
no
Pls make video on prepare mercuric nitrate solution 0.01 N in 1 ltr and standardized
Noted
We can use Aqua regia for plant samples eg; seed. for digestion and for mineral analysis in AAS? Please reply
You can use
Thank you
We have to use distilled or double distilled water?
Deionized Water
Is this procedure applicable for animal saltlick
Yes
Hi. How are you?
Can we use the way fo the soils samples to determine the total N?
No. You can use this method for nitrogen content.
You can determine the Nitrogen Content of your soil sample by the following video: th-cam.com/video/yJesl8hlbqs/w-d-xo.html
Can I prepare soil and plant sample in this process ??
Yes. You can.
What is procedure after sample preparation?
Run the sample in AAS, ICPMS or ICP-OES
Hello, I am pursuing a research, for which i need to estimate calcium in hair samples. can you kindly guide me on this. Kindly reply.
Thank you
Try this method for sample preparation
We can use only diacid Or triacid plz reply
Diacid
Please why is nitric acid used instead of other acids ?
There are some other methods where HCl & H2SO4 is used. But We developed this method using HNO3 combined with Perchloric Acid and found best digestibility of HNO3. Thats why HNO3 is used for the digestion.
How to check food products present in lead..,and how to check food products amount of lead
Prepare your sample as shown in this video and analyze using AAS.
Hello ma'am for sample digestion we can use aqua regia? Is that correct
Yes. You can
@@MicroChemsExperiments for mineral analysis in plant samples eg seed? Is that correct
How about analysis of heavy metals present in sausages
Sample sample preparation
Can this method used in blood serum heavy metal analysis?
Yes
@@MicroChemsExperiments our sample is in microvolume of serum only. Can you help us design our method sample preparation before can we proceed to the AAS anaysis?
Triacid Or diacid mixture
Diacid mixture
Sir, how to prepare urine sample for wet acid digestion? I have done blood sample but never done any urine sample, plz let me know
You can prepare urine sample in the same way described in this video. But take 10ml of urine sample in the flask instead of 2g. Rest of the procedure will be unchanged.
sir, I am using hot plate. Ok will take 10 mL urine.
@@ChemistryTipsTricks You can use Hot Plate but you have to digest the sample properly to get the accurate results
Whats the essence of glass beads pls does the glass bead prevent explosion also what is glass beads are the recoverable in solution or they have no effect on the digestion process or elemental losses or contamination
Pls give me some common examples of glass beads
Glass beads are used to prevent explosion which has no other effect on testing
nice video
what the nitric acid used for??
Nitric acid is used for the digestion of our sample.
i'm sorry i'm not that good at chem.. what is the purpose of digestion?
Geranally sample is a complex organic compounds. In the digestion process, each of the complex bonds are broken down in the presence of acid, heat and sometime catalyst. When all of the bonds are broken down, all of the minerals and heavy metals are released which can be quantified using AAS.
thank u so much, good luck for the next chemistry project!!!
@@shafadaraillona7850 Thank you so much for your support
What is the reason why glass beads underlie the digestion process?
Glass beads are used as an anti-bumping agents. Glass beads does not interfare the chemical reactions but prevents bumping the acid solution during heating at higj temperature.
Pls can i get an example
Ot its just broken glass i can buy from the market or i can break and pour into it
Please I want information about how digestion serum blood sample to assess zinc and selenium by AAS
Karntoka Ali, you can analysis your serum sample for Zinc & Selenium by following our video of WET DIGESTION. No modification is needed.
@@MicroChemsExperiments 🌺🌺🌺🌺 where's serum sample 😓 I just see powder sample
Sorry for stupid question ^_^
@@karntokaali9778 I didn't use serum sample in my video but if you want to analyze serum sample then you can follow the sample methodology shown in this video.
Hi, is the procedure suitable for cheese samples?
Yes.
@@MicroChemsExperiments thanks
@@asaadha80 Welcome
why did you use 65% v/v of the acids instead of a 100% concentrated ones?
65% nitric acid is enough for this purpose. But if you want, use 100%
There is no such thing like 100% nitric acid in liquid phase at STP. Period.
@@dwisworo332 You can use 65%
Just curious, did you dilute the nitric acid? The container and labe look like ones found on standard 65% Nitric acid GR by Merck.
@@dwisworo332 We didn't dilute the nitric acid. The purity of the nitric acid is 65%
How can we prepare meat sample for wet digestion? For AAS
Yes. You can
@@MicroChemsExperiments what can be procedure for that? My assumption is to dry the meat at 55 for 24-48h to remove moisture and next will be according to you
@@MicroChemsExperiments guide me
This is the only method for digestion? Is there any different methods
Yes there are other methods available but I made only one.
@@MicroChemsExperiments in those methods aqua regia is the one method? right?
How can you throw Acid like that, in the sink as well? It's bad for your pipe and the environment. I appreciate your video, but you must keep every aspect, especially health and safety issues
You are right but It is not possible to maintain everything (e.g: disposal of acid) in the video within a very short time. Because our main focus is to present the test method appropriately. Some times we pipette concentrated acids in the open place, without using the fume hood. We did like that to just shorten the video length. So please maintain health hazard issues at you laboratory while following my video.
Thank you so much for being with us.
We are a family.
Peace.
@@MicroChemsExperiments you are right ✨✨
can u plz share reference of a paper having this wet digestion method ?
This is in-house developed method
@@MicroChemsExperiments but u have adopted this method from some already existing method and then modified it by ur own. Can u plz elaborate about the parent method from which u hv modified this one ? kindly
@@teacherscommunity2552 Ok we will provide you later.
Please share detail protocol of water sample digestion I m lit bit confused
Take 2ml water sample in the digestion flask and proceed in the same way as described in this video
@@MicroChemsExperiments thank you
You are welcome
@@MicroChemsExperiments one more question ..if I will digest the sample using the method 3:1 Hno3/HCl..what should be volume of Hno3 and Hcl...becoz I don't have perchloric acid instead of that I will use HCl ..I m confuse about the volume of both chemicals
@@saminatahirkiani9806 HCL :HNO3 =3:1
Please mention the Reference
Inhouse validated method
References..??
No reference.
Kindly share Reference
It is a laboratory developed inhouse method
Can I digest the fish gills and liver with the same method using a hotplate?
Saeed Noori, yes, you can apply the same method. But you should use a digester because fish gills & liver may take longer period of time to digest and in hot plate technique nitric acid removes rapidly. So if you use hot plate then you have to add addition 10ml of nitric acid in sample and blank beakers in the middle of digestion period to prevent rapid dryness of the beakers.
@@MicroChemsExperiments thanks
@@shaysaeednoori Welcome
I tried to digest vegetable sample with sulphuric acid and hydrogen peroxide on hot plate for heavy metal analysis but no color change. Why???
Why did you try with Sulfuric Acid? Do according to this video and use nitric acid
Can we use the same method for detection of Heavy metals in egg shells of chicken?
Can we use HNO3 and HCl 3:1 or per chloric acid is compulsory???
On hot plate for how much time we have to heat for complete digestion?????
@microchem experiments ?
welcome lab first day noobs ... cleaning glassware for 8 hours a day before they let you do anything cuz you're on the bottom rung of the ladder and the newest staff there ..
Sorry, but I've observed a lot of experimental mistakes. It isn't acceptable.
May be we did a lot of mistakes, we could not do everything what we should do in the lab. This is just because we wanted to shorten our video length, thus skipped many things about the quality control of the test. We just focused on the main procedure of our target test. We are sorry for our mistakes. Plzz do the experiment in your lab correcting every mistakes based on your expertise. Thank you.
can I use this method for the determination of mercury in fish and plant samples?
Yes. You can
Is there any specific amount of deionized water to add, to conduct fe, zn,analysis? Reply please
You can volume 25ml or 50ml by adding DI water
What is the plant used?
You can use