This helped me so much. I would ask my professor for help, but expects me to already know this and I always get scolded, but you made me understand it so easily OMG.
thank you for this amazing video!! it actually not as hard as I thought it would be😭 but I came across this one question that made me confused because there is no similarly in fragments and the total is not the same, it would be helpful if you could briefly explain to me how to solve it🥹 Question uncut plasmid: 3.0 kb Xbal: 1.8 and 1.2 kb EcoRI: 1.6 and 1.4 kb Xbal + EcoRI: 1.0, 0.8 and 0.4 kb
In this question on the final digest you have more than one fragment of one size coming from the double digest...in such cases since there is only two cutting site you can start by placing 2 sites for xbal for 1.8 and 1.2 fragments. Now you need to place two more sites for ecor1. For that you refer the double digest and ecor1 fragments also.
In the double digest you can see 1 and 0.8 fragments that means the 1.8 fragment was cut into two pieces so you can place one site of ecor1 in between the xbal 1.8 fragment
For the second fragment of 1.2 if u subtract 0.4 from it you get 0.8.....so that means the other site is in between the 1.2 fragment but you cannot understand because it is also giving a 0.8 fragment...
You are provided results based on gel images and there we only come to know the different size fragments..based on intensity you can predict number of fragments but that does not work always...since you are not provided with the information that there are two 0.8 fragments you get confused. Always try to make the diagram and start with it. You will find the answer
In these simple questions it won't matter.... So there are always more possible options.... In some cases with multiple digests you can get better organisation of the fragments...but in real life, the exact sequence will be confirmed by dna sequencing if it's an unknown fragment.
For the purpose of solving this problem, any arrangement is acceptable till you get the right fragments upon digestion which shows that you understood the basic concept and can apply it in a laboratory
If you have English subtitles, it will be perfect, because the accent is very heavy, which is a bit difficult for people with poor English hearing. Anyway, Thank you so much!
we just randomly place them ensuring that the fragments obtained are correct. In some cases there are be more possibility for the fragment placement. Actual order can be known by sequencing.
If you have a circulation plastic containing a single ECORI site and you cut it with ECORI how many pieces of DNAwill be formed?what about circular plasmid containing ECORI Sites.?What about linear piece of DNA containing one ECORI Site
Please refer to my video which explains the basics. In circular DNA u get one piece for one site and if the DNA is linear then u get two pieces with one site
its confusing what if i have EcoRI cut in 2 places and BamHi cut in 3 fragment how am i supposed to do i only end up getting 1 ecori in the circular dna
If you have 2 ecori and 3 bam hi you will have total 5 cuts meaning 5 fragments..... normally in problema you will have atleast one enzyme which is making one cut...but even in the condition that you gave, you can randomly place the 2 ecori sites on the circular DNA....and then based on the double digestion fragments...you can mark the three bamHI sites.
I should have watched this earlier before I started crying...the video was really helpful
Thank you so much
Hahaha. Happy to help you out dear 😊😊😊
you have just saved my life after 1 hour of desperate searching in my way too expensive and unuseful literature, thank you!!
Happy to help 😊😊😊
CEO of restriction mapping🔬🧬
lol. Thank-you dear
Thank you so much, this video really helped me. I'm studying for my Molecular Biology exam and now I'm a little bit less scared :)
That's great to know. So happy to help 😊😊😊
I have exam tomorrow, you are my real teacher cause I did not learn anything from my lecturer. Thanks so much. God bless you❤
All the best for your exam. Hope this helps
30 minutes before my exam ur a lifesaver maam
Happy to help 😊😊😊
Thank you very much mam, I could have never understood restriction mapping without watching this video, thank you spookily much. It helped me a lot
😊😊😊
Merciiiii beaucoup vous sauvez mon année (you save my year) 💕💕💕
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wow. I'm confident about these problems now. The concept is so simplified. thanks
Thanks :-)
BioMagica Please make more videos. you are great
so much better than my genetics professor, you are amazing!!!!
Thank you. Happy to help 😊😊😊
Thx so much took me a whole hour trying to solve this problem, but you clarified it for me in five minutes!
Good Job maa'm..Thank u soo much...May Allah Bless u....Aameen...
Happy to help 😊😊😊
This helped me so much. I would ask my professor for help, but expects me to already know this and I always get scolded, but you made me understand it so easily OMG.
Don't worry about the scolding.... always give in your best. And thankyou so much. I'm so glad this helped you out
Best video . And very helpful
Happy to help 😊😊😊
Thank you so muchhhh! You don't know how much you have helped me with my exam 3 hours from now 😂 God bless your channel!
So happy to know that. All the best to you
I understood it very easily ..before it was running all over. thankyou it was very helpful
Happy to help 😊😊😊
explained a difficult topic in a very lucid manner, much appreciate
Thankyou ☺️
This video was helpful thank you
Happy to help 😊😊😊
thank you so much, i went from super confused to understanding everything perfectly! you're an amazing teacher
Happy to hear that! Thankyou so much
thank you miss, your video save my life for tomorrow final exam
Happy to help 😊😊😊
Thanks this video clarified my mind confused mind. I appreciate thanks
Happy to help 😊😊😊
Very clarifying video. Will watch once more!
:) thanks
Thaannkkkk yoouuu varyy vaaryyy muuucchhh♥️♥️♥️♥️
Happy to help 😊😊😊
Thank you so much this has really helped me understand restriction sites for my exams!
😊
Your video and explanation save many students’s life
Thankyou so much ☺️🤓👍
awesome explanation. you are the best :)
thankyou
Best video ever ❤️❤️❤️❤️❤️❤️❤️ ty soooooo much
Welcome
U Really simplified the concept in a short video. Thank you so much 😃😃😃😃
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very helpful thank you sooo much
Happy to help 😊😊😊
Thank you for this video! You clarified a lot of things I previously didn't understand 😊 keep up the good work!
Happy to help ☺️
Thank you soooo much for this video!!!!
ornela nono Happy to know it was helpful ^_^
Thank you soo much... You come as savior for students like me... Thank you soo much.
Happy to help. Thank you 😊😊
this video helped alot i watched some other this was the only one i understood thankyou for breakin it down simply.
So happy to know that it was useful to you
thank you so much for such crystal clear explanation.
Happy to help ☺️
Thank you so much. It was very helpful. ❤
Happy to help 😊😊😊
Explained this restriction mapping so well.
Thankyou
It was very helpful ,thank you so much 😊😊
Happy to help 😊 do share 😇
Thank you for such a clarifying video❤
Glad it was helpful!
U're the best 👌
thankyou
Thank you for making this make sense
Happy that it does 😇 do share 😊
video was helpful for last minute understanding of this before my quiz tomorrow thank you
Glad it helped! And all the best
Damm easy well done mam
Glad it was helpful 😊
It was so helpful!! Thank you so much ❤❤
Happy to help 😊😊😊
Do share 😇
This video ws really helpful thanks alot
Welcome 😊
So helpful! Thank you!
Happy to help 😊😊😊
thank you for this amazing video!! it actually not as hard as I thought it would be😭
but I came across this one question that made me confused because there is no similarly in fragments and the total is not the same, it would be helpful if you could briefly explain to me how to solve it🥹
Question
uncut plasmid: 3.0 kb
Xbal: 1.8 and 1.2 kb
EcoRI: 1.6 and 1.4 kb
Xbal + EcoRI: 1.0, 0.8 and 0.4 kb
In this question on the final digest you have more than one fragment of one size coming from the double digest...in such cases since there is only two cutting site you can start by placing 2 sites for xbal for 1.8 and 1.2 fragments. Now you need to place two more sites for ecor1. For that you refer the double digest and ecor1 fragments also.
In the double digest you can see 1 and 0.8 fragments that means the 1.8 fragment was cut into two pieces so you can place one site of ecor1 in between the xbal 1.8 fragment
For the second fragment of 1.2 if u subtract 0.4 from it you get 0.8.....so that means the other site is in between the 1.2 fragment but you cannot understand because it is also giving a 0.8 fragment...
You are provided results based on gel images and there we only come to know the different size fragments..based on intensity you can predict number of fragments but that does not work always...since you are not provided with the information that there are two 0.8 fragments you get confused. Always try to make the diagram and start with it. You will find the answer
Hope it helps 🤞🙏
Really helpful. Thank you so Much!!!
Happy to help ☺️
Thiss video is really nicee. Please make a video for mapping of linear DNA as well.
th-cam.com/video/HZKMzixy1ek/w-d-xo.html
Thankyou. Hope this helps
very helpful for my cellmol, thanks my dear!
Thankyou
thanks a lot, it really helped me
Glad it was helpful 😊
great stuff
thanks
Ty
Happy to help 😊
Thanks for this video
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Nicely explained.
Don't know why but it seems u made this video when u were in college.
Well partially correct. I made this video while i started teaching in college. It was only a year after i completed my masters, so quite close.
Happy to know it was useful 🐱
Nice video. Very helpful.
Glad you liked it. Do share
Thankyou so much✨💛
Happy to help 😊 Do share with others.
Thank you so much for this❤ it helped a lot.
You're so welcome!
Exceptional thank u ma'am jai jagannath
Thank you too
thank you. Very useful
You are welcome!
Please do share the video
Wow ! Very helpful thanks
Thanks
Bless🙏
Happy to help 😊
Wunderschöne 👏👏👏👏👏👏👏👏
Thankyou ☺️
Thank you!
Happy to help 😊 do share 😇
Nic plz upload all these lecture
Glad you liked it. Do share
😍
😇
THis is great
Glad you liked it. Do share
THANKSS MAM 😍😍💥💥💯🙏🙏
Welcome
Nice...
Glad you liked it. Do share
SO GOOD THANKYOU
happy to help. Do share
great one
Thankyou 😊
nice video
Thanks
thank you soo much but what if i change the direction of pieces
In these simple questions it won't matter.... So there are always more possible options.... In some cases with multiple digests you can get better organisation of the fragments...but in real life, the exact sequence will be confirmed by dna sequencing if it's an unknown fragment.
For the purpose of solving this problem, any arrangement is acceptable till you get the right fragments upon digestion which shows that you understood the basic concept and can apply it in a laboratory
Hope it helps. Thankyou
If you have English subtitles, it will be perfect, because the accent is very heavy, which is a bit difficult for people with poor English hearing. Anyway, Thank you so much!
Yes i get it. I have uploaded subtitles on most of the videos. Trying to add for all soon 🙂
Can't thank you enough
Happy to help 😊 all the best 👍
Thanks
Welcome
😍😍
Very nice ❤
Thanks
Thank you so much❤
You're welcome 😊
The music in the video ws relaxing...
Thankyou
Thanks mam
welcome
Thanks a lot
Happy to help
Thank you so much h❤❤❤
Welcome
Thanku soo much
Most welcome 😊
THANK YOU
Glad you liked it. Do share
How can you do this if one of the digests has 2 fragments, and the other 3
Hi ,If I want to learn restriction mapping by myself how can I learn it ?
See videos and practice on your own you will get it once you practice enough
Helpful
Thankyou
But how do you know the order of the 6/4/3 fragments?
we just randomly place them ensuring that the fragments obtained are correct. In some cases there are be more possibility for the fragment placement. Actual order can be known by sequencing.
Thanx 😊
Welcome
What if you are combining more than one map? Help would be appreciated!
Can you send an example so it would be easier to help you out. You can email to biomagica.solutions@gmail.com
If you have a circulation plastic containing a single ECORI site and you cut it with ECORI how many pieces of DNAwill be formed?what about circular plasmid containing ECORI Sites.?What about linear piece of DNA containing one ECORI Site
Please refer to my video which explains the basics. In circular DNA u get one piece for one site and if the DNA is linear then u get two pieces with one site
th-cam.com/video/HZKMzixy1ek/w-d-xo.html
Thanks mam you are very nice in nature
Plz can you help me in relative problems ?
Yes sure
@@BioMagica how can I connect with you ?
@@BioMagica plz replay to me
@@manarabual-rub8458 Biomagica.solutions@gmail.com
its confusing what if i have EcoRI cut in 2 places and BamHi cut in 3 fragment how am i supposed to do i only end up getting 1 ecori in the circular dna
If you have 2 ecori and 3 bam hi you will have total 5 cuts meaning 5 fragments..... normally in problema you will have atleast one enzyme which is making one cut...but even in the condition that you gave, you can randomly place the 2 ecori sites on the circular DNA....and then based on the double digestion fragments...you can mark the three bamHI sites.
Try referring to a basic video..I'll add a link here
th-cam.com/video/HZKMzixy1ek/w-d-xo.html
If you still have doubts. You can email the particular problem at biomagica.solutions@gmail.com
Thank you very much I'll try that right now
Thank youuuuu
Welcome
when ur teacher takes a whole lesson to explain this concept and u take 6 min
lol.Thankyou. Glad it was useful to you. do share
Sooooooo* much
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I cannot understand what you are saying... i need subtitle
Sure. Will add subtitles asap.
16/05/2023
Lol
copied from Shomu's Biology xD
Anyone who knows how to solve it will definitely think in this direction 😊😊😊
@arindam This video is so much better, and simplified. Well obviously the concepts will be the same. But the content is consized and easy to follow.
@@BioMagica I agree. Thank you for making these videos. They are to the point and very easy to understand. Keep up the good work
Thanks, this helped so much.
Happy to help 😊 all the best 👍
Do share 😇