I am just starting my master's and this explanation was super valuable. I really appreciate the time you put into these videos and making science easy to understand. Keep up the great work you do!
Hello, my laboratory is going to begin a new line of research in the production of recombinant proteins in insect cells. We are starting and looking for materials. Could someone tell me where I can find those screwed glass flasks? Are the plugs sold separately? Are they ventilated? Thanks, very good video.
How important is determining virul titer before infecting the large scale culture? Can i just add the filtered medium from a smaller infected culture (100mL) into a larger uninfected culture (800mL) and see what happens?
Update: I ended up just adding the P3 supernatant containing the virus (stored for over 10 years at -80°C). I had no idea when to harvest though as the only notes I had were to harvest 48 to 72 hrs post infection or when cell viability decreased to ~70%. My cells were over 94% after 72hrs. But cell diameter had significantly increased ~34%. Turns out this is a good indicator of expression. I harvested but let a little bit of the culture continue on and cell size had leveled off and viability dropped to 92% 24 hrs later. I got a good yield (better than the last person) and purity was better. Activity was also good. Not bad for my first time, I'm glad I didn't wait for viability drop to 70% as I would have had to keep checking the cells throughout the weekend. Your videos were a great help, thank you.
I've got a job interview this week for a job involving baculovirus expression and this has really helped me feel more prepared, thank you!
Awesome! Best of luck!
I am just starting my master's and this explanation was super valuable. I really appreciate the time you put into these videos and making science easy to understand. Keep up the great work you do!
Thank you! So glad it was helpful!
Nice explanation. I always feel like I should be walking on a treadmill while I watch your videos. :)
Thanks!
This was very helpful, thank you!
Glad it was helpful!
Thank you so much
This is absolutely incredible and SO informative!!
Thank you so much. Great to hear
Hello, my laboratory is going to begin a new line of research in the production of recombinant proteins in insect cells. We are starting and looking for materials. Could someone tell me where I can find those screwed glass flasks? Are the plugs sold separately? Are they ventilated? Thanks, very good video.
I don't know sorry. And thanks!
Thank you so much. So helpful!
Glad it was helpful!
Great video! Thanks for the information
Thanks!
Great video!
thank you!
How important is determining virul titer before infecting the large scale culture? Can i just add the filtered medium from a smaller infected culture (100mL) into a larger uninfected culture (800mL) and see what happens?
I didn't usually titer, just looked under the microscope at how green the cells were and based the amount off of that. Good luck!
Update: I ended up just adding the P3 supernatant containing the virus (stored for over 10 years at -80°C). I had no idea when to harvest though as the only notes I had were to harvest 48 to 72 hrs post infection or when cell viability decreased to ~70%. My cells were over 94% after 72hrs. But cell diameter had significantly increased ~34%. Turns out this is a good indicator of expression. I harvested but let a little bit of the culture continue on and cell size had leveled off and viability dropped to 92% 24 hrs later. I got a good yield (better than the last person) and purity was better. Activity was also good. Not bad for my first time, I'm glad I didn't wait for viability drop to 70% as I would have had to keep checking the cells throughout the weekend. Your videos were a great help, thank you.