Thanks so much! Glad I could at least help some people! And I just looked and it looks like a lot more people have actually watched it, the lower number's for "likes"
Thank you so much! I'm gonna start a new job at a lab in Munich in protein purification and I'll work in the department of baculovirus expression vector systems so this was extremely helpful for me to understand the overall work that I'll be doing from the following year on!
Fantastic video. I am trying to get a stock of Bacmid DNA (from a mimiprep) but got zero colonies. My Bacmid contains already my gene of interest. I tried to transform XL10-Gold cells and failed (Kanamycin + Gentamycin antibiotic selection). Any idea to how to get a stock of Bacmid? Am I using the wrong Bacteria? should I use dh10bac again to make a stock of the Bacmid? Should I use Kanamycin only when trying to grow the bacteria?
Thank you so much for such a great interesting video! 🤩Could you please answer a couple of questions? (I really want to understand this topic) Why can bacmid be hosted in both bacterial and insect cells and why the acceptor plasmid can't? And what is meant by "hosting" - both replication and transcription or not?
thank you! to be “hosted” means the cells will at least maintain it and pass it on, which requires replication. therefore, the plasmid or bacmid needs to have an origin of replication initiation (ORI) compatible with that cell type and/or the cells need to be able to produce virus that will replicate itself. the bacmid is compatible this way with both bacterial and insect cells. it has a bacterial ori and once you get it into insect cells the virus will be produced and replicate full virus. the birus doesn’t get made in bacteria cells because there aren’t compatible promoters for transcription the acceptor is the bacmid, so I’m confused by that question, sorry. hope the rest helps!
@thebumblingbiochemist Thanks!! And...maybe it will be a very stupid question, but why can't we just immediately insert the gene into the bakmid in a test tube, for example? And thus skip the step with bacteria
Sorry to see that only thirty-something people watched this movie. You know, this movie is really great: well structured and extremely informative!
Thanks so much! Glad I could at least help some people! And I just looked and it looks like a lot more people have actually watched it, the lower number's for "likes"
Thank you so much! I'm gonna start a new job at a lab in Munich in protein purification and I'll work in the department of baculovirus expression vector systems so this was extremely helpful for me to understand the overall work that I'll be doing from the following year on!
Awesome! Here's an updated version too: th-cam.com/video/tPkkFFYLGRs/w-d-xo.html
Best of luck with the new job!
I think this video is pretty helpful! I am recently in a lab and I am glad that I can find this video with great explanations.
Great to hear -thank you! And best of luck with your research
@@thebumblingbiochemist Thank you so much! Your video helps me to connect lots of pieces of information together! I feel I understand better now. 😊
Excellent overview of Baculoviral expression
Thank you!
Fantastic video! The graphics used paired very well with your explanation, and I thoroughly enjoyed the video.
Thank you!
wow really enjoyed the video. it was hillarious when one cell got sucked up into the syringe
thank you!
I like the way u teach
Thanks so much!
very useful and explicit.. thank you
Thank you!
Great Video! Thanks!
Thank you!
Fantastic video. I am trying to get a stock of Bacmid DNA (from a mimiprep) but got zero colonies. My Bacmid contains already my gene of interest. I tried to transform XL10-Gold cells and failed (Kanamycin + Gentamycin antibiotic selection). Any idea to how to get a stock of Bacmid? Am I using the wrong Bacteria? should I use dh10bac again to make a stock of the Bacmid? Should I use Kanamycin only when trying to grow the bacteria?
Not sure sorry, and it's been a while since I've worked with those. Best of luck!!
Thank you so much for such a great interesting video! 🤩Could you please answer a couple of questions? (I really want to understand this topic) Why can bacmid be hosted in both bacterial and insect cells and why the acceptor plasmid can't? And what is meant by "hosting" - both replication and transcription or not?
thank you! to be “hosted” means the cells will at least maintain it and pass it on, which requires replication. therefore, the plasmid or bacmid needs to have an origin of replication initiation (ORI) compatible with that cell type and/or the cells need to be able to produce virus that will replicate itself. the bacmid is compatible this way with both bacterial and insect cells. it has a bacterial ori and once you get it into insect cells the virus will be produced and replicate full virus. the birus doesn’t get made in bacteria cells because there aren’t compatible promoters for transcription the acceptor is the bacmid, so I’m confused by that question, sorry. hope the rest helps!
@thebumblingbiochemist Thanks!! And...maybe it will be a very stupid question, but why can't we just immediately insert the gene into the bakmid in a test tube, for example? And thus skip the step with bacteria
you need the bacteria to do the recombination to make it and then make lots of copies of it
@@thebumblingbiochemist Thank you😊
@@astroseedling8831 happy I could help