Thank you sir. You explained it so nicely. I was struggling to solve second order polynomial equation, but I was not aware that it's so easy to solve it by excel.
😃😃Thank You Protocolplace. This method really helped me alot, though i have a long way to go. Wanted to ask, Why some of my unknown concentration calculated are Negative ?
Thank you for this video. I wanted to know why some of my OD sample value bring cut off by a line?? I am still new in this 😅 I can't seem to find out why it's like this
Thank you Dear! Absorbance and concentration is directly proportional but here I have seen the revers. could you say something on the reason why this happen?
Hi Youssef, thank you for the great videos. One thing that puzzles me is the plotting of the graph for extrapolating the unknown concentraions. Usually we plot Standard concentrations on X axis and variables like absorbance on the Y axis. In this tutorial, it is the other way around. Could you kindly provide an explanation for this. Thank you
Hai thanks for the Video it helped me lot to understand , kindly let me now what if r2 value don't meet to .9 that is not closer to 1 in such result how to evaluate . And if the absorbance lower r greater to you std absorbance what to do ,how to dilute and measure the assay kindly help me in this to understand better .
hi, sorry, but I don't understand how I should interpret the concentration after I calculated them? To what I should compare them? if I need to know the severity of the sample, what's the limit I should compare to? Is it positive control? and how I say this sample is negative? is it negative with its concentration equals/less the negative control? or should I compare them to the standards? please help me :(( I have an ELISA report to submit
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Thank you sir. You explained it so nicely. I was struggling to solve second order polynomial equation, but I was not aware that it's so easy to solve it by excel.
Thank you very much! You just can`t imagine how this video helped me!
This video was very very helpful ! Thank you !!
You need to do a serier on data-analysis basics. My university professor never explained this, just assuming we know it, failing 80% of class.
😃😃Thank You Protocolplace.
This method really helped me alot, though i have a long way to go.
Wanted to ask, Why some of my unknown concentration calculated are Negative ?
Million thanks! I found this video very helpful
Thank you very much. That saved my life.
Wonderful video. Extremely well explained. Special thanks for explaining the calcs. Great job!!! Thumbs up!
You could have also shown us how to calculate intra-assay and inter-assay coefficient of variations
Very useful! many thanks!
What to do if curve fit equation has E
all fine until you plugged in 1/abs to x values.. can you explain that a little..
very well described 👍👍👍👍
Thanks a million! I found this video very helpful
The video made my day. Thank you
Great Video!!!!
Thank you for this video. I wanted to know why some of my OD sample value bring cut off by a line?? I am still new in this 😅 I can't seem to find out why it's like this
Hi, how can I find the reference of that when data that are greater or smaller than standard ranges will be deleted?
Many thanks
Nizar Hussein
very beautifully explain sir thanks
Thank you Dear! Absorbance and concentration is directly proportional but here I have seen the revers. could you say something on the reason why this happen?
This is competitive ELISA so it is not directly proportional. Watch this th-cam.com/video/Kb26nQVMHds/w-d-xo.html.
Thank you!
Hi Youssef, thank you for the great videos. One thing that puzzles me is the plotting of the graph for extrapolating the unknown concentraions. Usually we plot Standard concentrations on X axis and variables like absorbance on the Y axis. In this tutorial, it is the other way around. Could you kindly provide an explanation for this.
Thank you
I believe it's so you can easily solve for Y (concentration) using the polynomial equation generated
this is more suitable for measuring protein concentration. ELISA data with STD with known concentrations???
Hai thanks for the Video it helped me lot to understand , kindly let me now what if r2 value don't meet to .9 that is not closer to 1 in such result how to evaluate . And if the absorbance lower r greater to you std absorbance what to do ,how to dilute and measure the assay kindly help me in this to understand better .
Hye! can i use this method for my sandwich ELISA data ?
Very useful video thank you so much..
hi, sorry, but I don't understand how I should interpret the concentration after I calculated them? To what I should compare them? if I need to know the severity of the sample, what's the limit I should compare to? Is it positive control? and how I say this sample is negative? is it negative with its concentration equals/less the negative control? or should I compare them to the standards? please help me :(( I have an ELISA report to submit
Great Video, Thank you so much.
Thanks, Really Great !
wish you'd do some tutorials for excel tests (fisher test etc.), since your explanations are great
very nice sir, thank you
Can cElisa differentiate the vaccinated & Infected patients
hi i found "E" on my equation. what does it mean?
Della Dedel E means exponential,,ie. exp in Excel
Can I have your mail Id
I hate elisa!!! #metabolism
Best tutorial. Thank you very much ❤