ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism

[@erythreal7770]

I'm so thankful for your ELISA tutorials! Without them I'd be failing my uni assignments, thank you so much!!!

[@Protocolplace-about]

I'm glad the video is helping! Best of luck with your studies : )

[@rimapant0]

Explanation is very clear and simple. This tutorial series is very helpful.
Thanks for the all the effort and time you putting into it.
Waiting to see more.

[@anata110]

Your video presentation is very clear, easy to understand. Very helpful, especially on how to deal with the data. Thanks a lot.

[@Smiles5s5s]

Wow. This was the best tutorial of anything I've ever seen before.

[@royzhang5192]

thanks so much for this tutorail and your selfless dedication

[@zeelbhatia6187]

thank you for this video. you made ELISA assay easy.

[@sadehavvad]

Thank you! I am very happy that I made it all by myself :)

[@carlitosalex2006]

This video was sensational, very clear and objective. Even with a previous version of the GraphPad Prism, the explanation was very helpful. Thanks a lot !!!

[@hassertoo1424]

Hi. Do you know If I can do the analysis in graphpad prims 5?
The thing is, in this version there isn't interpolate standard curve option 😪😪 so if you know please let me know. Thanks.

[@nurainmfuseini]

youssef! Glad to see you are doing great things! hope all is well!

[@sheilaspadaphd9009]

Hi, many thanks for your help! quick question, is the blank subtracted? Thanks!

[@carinebokop3496]

Hello, thank you so much for the videos, Is there a following tutorial ? tutorial 7? Or are there other videos on Gaphpad prism beside tutorial 5 and 6?

[@missroua6862]

thanq so much, still waiting for the sequel :)

[@mustanghmt]

The tutorial is very helpful , could you please explain (the next step) how to use the obtained concentration and the other data to graph the quantified protien and show the error bars

[@BenjaminMatei-v8w]

That was really helpful. Thank you so much! The problem I am experiencing is that Prism would just "make up" values for the readings that were negative or 0. Even though in one run I had a zero standard given with the absorbance and concentration of zero it would calculate a concentration of 50 for another sample that had the same absorbance?! Any suggestions on how I can prevent this, so that my interpolation will also just not give me any result for the samples at or below 0?

[@fml18]

Thank you for help us!

[@weiliu3655]

Really helpful thank you very much

[@medicalresearchcentre-kast9445]

Thank you for the tutorial. I use graph pad Prism 8. Please help me as my Table of Results is missing so I cannot determine R2

[@rahamanahmed3681]

Thank you. Helpful

[@Exculibar7]

Thanks, help a lot!

[@hassertoo1424]

Can someone tell me if it isn't the opción of interpolate standard curve in graph pad prims how can I do this analysis? Thanks

[@shoxanabdullah7151]

please can you give explanation for GraphPad 5 because I did what you said but not give me correct number of concentration of cytokines I chose (analyse->nonlinear curve fit-> interpolate unknown samples-> ok ) but no result when I chose linear I get lower number

[@mikenaughton]

Most immunoassays work best using non linear data, most commonly a 4 or 5 parameter logistic regression with a 1/y2 weighting factor applied.

[@peterfarsta]

Thanks alot. This video helped me heaps!

[@faithuwadiae5609]

Thanks! This video helped so much!

[@NurgisaRametov]

Thanx, but this website don't work in Kazakhstan. Can you fix it?

[@Unpopular-channel]

thank you, exellent .can you do pcr the same way please.

[@kushalkatuwal3900]

cannot use free trial?

[@zeeshan2k5]

hello dear, I need your help in graphpad software its very little help?

[@xiadong2295]

Very helpful! Thank you

[@worldofinformation815]

Thank you 👍✔

[@gengpan]

thumb up. if the curve is not good one what should we do then?

[@SadaqurR]

Try another standard curve model from the "Interpolate a Standard Curve" option

[@immunologistkhalidaljebory6916]

thank you for this lecture

[@gengpan]

if the unkown Y value is out of the standard cure range, is there any method to predict the X value without redo the ELISA?

[@meghapatel1979]

dilute the sample

[@gengpan]

@@meghapatel1979 there was no more samples. anyway method was found.

[@SadaqurR]

@@gengpan Would you please share the method?

[@gengpan]

Sadaqur Rahman I don’t remember clearly. I changed x to log scale and cure became a straight line. Then any point lay on the extension of the straight line is predictable.

[@SadaqurR]

@@gengpan That means the graph becomes semilogarithmic (the manual with my kit also said to fit as semilogarithmic curve). Did you use Microsoft excel or any other software? Thanks for the indication. That might help a lot.

[@0457655]

wow this video helped me so much !

[@chantellelehone9677]

THANK YOU!!!

[@VanessaOliveira-nd7yh]

What if my data doesn't fit the graph?

[@worldofinformation815]

thanks

[@Yodit187]

Great!!! Thank you

[@Irfan-zy9xu]

i though elisa reader itself computes all the data for you, isnt it so

[@claudiaespinosagarcia546]

VERY helpful. Thank you!

[@TanTan-ch3vq]

Avery helpful, thank you very much!

[@kathyguo1450]

Very helpful! Thank you very much!