This video was sensational, very clear and objective. Even with a previous version of the GraphPad Prism, the explanation was very helpful. Thanks a lot !!!
Hi. Do you know If I can do the analysis in graphpad prims 5? The thing is, in this version there isn't interpolate standard curve option 😪😪 so if you know please let me know. Thanks.
That was really helpful. Thank you so much! The problem I am experiencing is that Prism would just "make up" values for the readings that were negative or 0. Even though in one run I had a zero standard given with the absorbance and concentration of zero it would calculate a concentration of 50 for another sample that had the same absorbance?! Any suggestions on how I can prevent this, so that my interpolation will also just not give me any result for the samples at or below 0?
Hello, thank you so much for the videos, Is there a following tutorial ? tutorial 7? Or are there other videos on Gaphpad prism beside tutorial 5 and 6?
please can you give explanation for GraphPad 5 because I did what you said but not give me correct number of concentration of cytokines I chose (analyse->nonlinear curve fit-> interpolate unknown samples-> ok ) but no result when I chose linear I get lower number
Sadaqur Rahman I don’t remember clearly. I changed x to log scale and cure became a straight line. Then any point lay on the extension of the straight line is predictable.
@@gengpan That means the graph becomes semilogarithmic (the manual with my kit also said to fit as semilogarithmic curve). Did you use Microsoft excel or any other software? Thanks for the indication. That might help a lot.
Explanation is very clear and simple. This tutorial series is very helpful. Thanks for the all the effort and time you putting into it. Waiting to see more.
The tutorial is very helpful , could you please explain (the next step) how to use the obtained concentration and the other data to graph the quantified protien and show the error bars
I'm glad the video is helping! Best of luck with your studies : )
I'm so thankful for your ELISA tutorials! Without them I'd be failing my uni assignments, thank you so much!!!
Your video presentation is very clear, easy to understand. Very helpful, especially on how to deal with the data. Thanks a lot.
This video was sensational, very clear and objective. Even with a previous version of the GraphPad Prism, the explanation was very helpful. Thanks a lot !!!
Hi. Do you know If I can do the analysis in graphpad prims 5?
The thing is, in this version there isn't interpolate standard curve option 😪😪 so if you know please let me know. Thanks.
thanks so much for this tutorail and your selfless dedication
thank you for this video. you made ELISA assay easy.
youssef! Glad to see you are doing great things! hope all is well!
Thank you! I am very happy that I made it all by myself :)
That was really helpful. Thank you so much! The problem I am experiencing is that Prism would just "make up" values for the readings that were negative or 0. Even though in one run I had a zero standard given with the absorbance and concentration of zero it would calculate a concentration of 50 for another sample that had the same absorbance?! Any suggestions on how I can prevent this, so that my interpolation will also just not give me any result for the samples at or below 0?
Hello, thank you so much for the videos, Is there a following tutorial ? tutorial 7? Or are there other videos on Gaphpad prism beside tutorial 5 and 6?
thanq so much, still waiting for the sequel :)
VERY helpful. Thank you!
Thank you for the tutorial. I use graph pad Prism 8. Please help me as my Table of Results is missing so I cannot determine R2
Thank you for help us!
Thanks alot. This video helped me heaps!
Thanks! This video helped so much!
Thank you. Helpful
please can you give explanation for GraphPad 5 because I did what you said but not give me correct number of concentration of cytokines I chose (analyse->nonlinear curve fit-> interpolate unknown samples-> ok ) but no result when I chose linear I get lower number
Most immunoassays work best using non linear data, most commonly a 4 or 5 parameter logistic regression with a 1/y2 weighting factor applied.
Thanks, help a lot!
Can someone tell me if it isn't the opción of interpolate standard curve in graph pad prims how can I do this analysis? Thanks
Very helpful! Thank you
wow this video helped me so much !
Thank you 👍✔
THANK YOU!!!
thank you, exellent .can you do pcr the same way please.
cannot use free trial?
Thanx, but this website don't work in Kazakhstan. Can you fix it?
Hi, many thanks for your help! quick question, is the blank subtracted? Thanks!
thumb up. if the curve is not good one what should we do then?
Try another standard curve model from the "Interpolate a Standard Curve" option
What if my data doesn't fit the graph?
if the unkown Y value is out of the standard cure range, is there any method to predict the X value without redo the ELISA?
dilute the sample
@@meghapatel1979 there was no more samples. anyway method was found.
@@gengpan Would you please share the method?
Sadaqur Rahman I don’t remember clearly. I changed x to log scale and cure became a straight line. Then any point lay on the extension of the straight line is predictable.
@@gengpan That means the graph becomes semilogarithmic (the manual with my kit also said to fit as semilogarithmic curve). Did you use Microsoft excel or any other software? Thanks for the indication. That might help a lot.
i though elisa reader itself computes all the data for you, isnt it so
Very helpful! Thank you very much!
Explanation is very clear and simple. This tutorial series is very helpful.
Thanks for the all the effort and time you putting into it.
Waiting to see more.
The tutorial is very helpful , could you please explain (the next step) how to use the obtained concentration and the other data to graph the quantified protien and show the error bars
hello dear, I need your help in graphpad software its very little help?
Really helpful thank you very much
Avery helpful, thank you very much!
thanks
Wow. This was the best tutorial of anything I've ever seen before.
Great!!! Thank you
thank you for this lecture