How to Make Thin Layer Chromatographic Plate (TLC) | Thin Layer Chromatography | क्रोमैटोग्राफी

Thin Layer Chromatography (TLC) = How to Make Thin layer Chromatographic Plate (ENGLISH) • Thin Layer Chromatogra...
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Chromatography- Chromatography is one of those simple but powerful techniques to separate the mixture into its individual component. Chromatography has two parts in its name- chrome- means colour and graphy means a visual identification. there are several types of chromatography like- (1) Paper chromatography (2) Thin layer chromatography (3) Ion exchange chromatography (4) HPLC- High-performance liquid chromatography. Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.
TLC plates, preferably ready made with a stationary phase: These are stable and chemically inert plates, where a thin layer of stationary phase is applied on its whole surface layer. The stationary phase on the plates is of uniform thickness and is in a fine particle size.
TLC Plate preparation consists of following steps-
1- Selection and cleaning of the glass plate for Thin-layer Chromatography (TLC)
2- Drying of plate Thin-layer Chromatography (TLC)
3- Making a slurry of silica gel and water Thin-layer Chromatography (TLC)
4- Transfering the slurry into a beaker
5- Transferring of slurry on a glass plate Thin-layer Chromatography (TLC)
6- Uniformly distribution of a slurry
7- Allow the slurry to dry at room temperature
8- Transfer the glass slide on the oven to make it dry
9- Dry and activate a glass plate Thin-layer Chromatography (TLC)
10- Use as per instruction Thin-layer Chromatography (TLC)
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