Bro thank you SO much, I have been trying to figure it out of literature for over a week but to no avail. The fact that this whole process takes less than 5 minutes is mind blowing. Thank you for your explanation.
Me not expressing my gratitude would be wrong! I really appreciate this video. So simple, so quick and so straight forward. I've been trying to figure this out for the past three days and yet, you've helped me in less than 5min. Really appreciate it bro!!🙏 Keep it up👌
Thank you, great video! But I don't understand how I should interpret the concentration after I calculated them? To what I should compare them? if I need to know the severity of the sample, what's the limit I should compare to? Is it the positive control? and how I say this sample is negative? is it negative with its concentration equals to the negative control or less? please help me :(( I have an ELISA report to submit
The trendline should pass through origin as per the operating procedure. When chosing the option to display R^2 in the excel, there is an option to set intercept. Make it 0. The final equation will be of form y = mx. That is the calibration equation which needs to be used for determining the unknown concentration. Hope it answer your question. Enjoy learning!
this might be because you have not diluted your protein standard to 1mg/ml if this is the case your absorbance value for your protein standard would be higher than 1 (into the range of 2-4). This is likely the case that results in a negative value.
how can i convert this concentration into % in order to compare it with protein content obtained by kjeldahl method please help me with the formula its urgent
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Bro thank you SO much, I have been trying to figure it out of literature for over a week but to no avail. The fact that this whole process takes less than 5 minutes is mind blowing. Thank you for your explanation.
Me not expressing my gratitude would be wrong! I really appreciate this video. So simple, so quick and so straight forward. I've been trying to figure this out for the past three days and yet, you've helped me in less than 5min. Really appreciate it bro!!🙏 Keep it up👌
Thank you so much! This was so useful and I can't believe it took me so long to work out
You are life saver ❤...I was very confused for this calculation but you saved me.. Thanks a lot sir🙏🥰
Thank you saved a lot of time doing my homework !
ahh thats amazing and so quick, cant believe is wasted a week on sorting it out, thank you
Thank you soooo much!! It's straightforward and easy to understand 😊
Thank you very much sir. God bless you 💝
This is so helpful
Can't thank enough🙏
Thank u so much. I have to do this on next monday.
Thanks a lot, this literally saved my life
thank u for vedio please tell can we use your standard curve for analysis
Thank you so much sir ....very helpful
Thank you sir , Please make more videos on experimental data analysis like western blotting and PCR.
Nice Job!!
its very useful for me thanks alot
very informative
thanks sir for detailed explanation
Thank you. Consider Subscribing.
Thanku so much sir..it helped a lot
you’re amazing. thank you
Exam m computer ni milega intercept or slope value calculate krne k liye, manual calculate kr k b dikhana tha
thank you so much. life saver!
Such helpful video 👍🏻
Nice explanation
And very useful video
Thank you.
this was such a help. Thank you very much
Thank you, its great video
You're amazing man
Thank you for making this!!!
THANK YOU👍🏼👍🏼
Amazing video thanks a lot!
this really helped, thank you so much!
Hi I have a doubt this same for unknown concentration of protein sample by using bca assay and how can I write final results
Damn!! This is great... Thanks a lot
Sr how you calculated intercept value
If straight line equation is y=mx-c..this standard curve also considered as correct or not??..please answer sir
well explained
In case of undiluted samples, shall we correct the OF with the blank or not necessary?
Sorry OD value
super helpful, thank you!
Thank you, great video! But I don't understand how I should interpret the concentration after I calculated them? To what I should compare them? if I need to know the severity of the sample, what's the limit I should compare to? Is it the positive control? and how I say this sample is negative? is it negative with its concentration equals to the negative control or less? please help me :(( I have an ELISA report to submit
The trendline should pass through origin as per the operating procedure. When chosing the option to display R^2 in the excel, there is an option to set intercept. Make it 0. The final equation will be of form y = mx. That is the calibration equation which needs to be used for determining the unknown concentration.
Hope it answer your question. Enjoy learning!
It’s a great video! Thank you so much! But when I calculated my protein concentration, it came out negative. Does anyone know what it means? 😢
me too
this might be because you have not diluted your protein standard to 1mg/ml if this is the case your absorbance value for your protein standard would be higher than 1 (into the range of 2-4). This is likely the case that results in a negative value.
thankyouuuuuuuuuuuuuuuuuuuuuuuuu sir 🤗
super usefull!!!
Very nice...
how can i convert this concentration into % in order to compare it with protein content obtained by kjeldahl method please help me with the formula its urgent
how to calculate phenolic content by Spectroscopy ?
thank you
I got negative values while substracting....what does it mean?
Can you give me the formula for determination of protein
@Bio-resource can you please tell me how to convert from ug/ml to ug/g of FWM, it's urgent please help me brother
what if the od of unknown samples is out of the od of standard reagents?
Hi,
You can dilute the unknown sample to fall into the standard's range.
👍🏼🙏🏻
Can you explain how do you find the number 0.34, .132
❤
Where is the negative 0 ug/mL control?
thanks
👍👍👍👍😍😍
Its not a standard curve if you use a linear fit...
you need to adjust the content
Hi.. didn't get you. Could you please clarify?
Bunyi video tidak bagus
its not clear you have to details more..i dont like your explanation
Thank you so much. This is so amazing
Thank you