You count the colonies that form in the plate, that is why it is called plate count. With the colonies found on the plate you calculate the CFU (colony forming unit) and have an estimate of viable cells available per mL from your original sample.
Right down to the roof of the R&D complex in Hercules, CA. www.google.ca/maps/@38.0255571,-122.2766188,3a,75y,323.16h,87.15t/data=!3m7!1e1!3m5!1sZ7M37LTwRXIynelM8B9yag!2e0!6s%2F%2Fgeo1.ggpht.com%2Fcbk%3Fpanoid%3DZ7M37LTwRXIynelM8B9yag%26output%3Dthumbnail%26cb_client%3Dmaps_sv.tactile.gps%26thumb%3D2%26w%3D203%26h%3D100%26yaw%3D70.26178%26pitch%3D0%26thumbfov%3D100!7i16384!8i8192?hl=en
Dear Bio Rad Thank you for this videos, is it possible to upload let say part 2 video to this one showing who to count the plates after incubation? However some people describe below who to count the plate, I do appreciated, but it will be more complete to add plate colonies counts video to this one! many thanx to all
You count the number of colonies that grow there; that gives an estimation of the number of bacteria in the volume of liquid you inoculated the plate with. To get the number of bacteria in 1 ml, you need to account for the volume you put on the plate and the dilution. If you put 0.1 ml on the plate, you need to times that by 10 to get it in ml. If you were counting 10^-5 dilution, you need to times it by 10^5 to get it in 10^1. Cancel those out and you have Colony forming units / ml.
Hi, Sana! To count colonies, we recommend placing the plate lid-side down (so the agar is up and you're looking at agar underside) on a background that gives you good contrast between the agar and the colonies - for white colonies, a black background works well. Using a felt-tipped pen, dot each colony as you count; by dotting you'll know if you already counted a colony. If the plate has a lot of colonies, use the marker to draw lines on the backside of the plate to mark four equally-sized quadrants, count the colonies in one of the quadrants, and multiply by four for an estimated number of colonies on the plate.
You count the colonies that form in the plate, that is why it is called plate count. With the colonies found on the plate you calculate the CFU (colony forming unit) and have an estimate of viable cells available per mL from your original sample.
can you add a video that explains what do you do afterwards? how to analyze the plates?
thank you, I need a refresher and your videos have helped enormously.
Sameeeee.
They really like the color green.
Ghtvgbbt
Right down to the roof of the R&D complex in Hercules, CA.
www.google.ca/maps/@38.0255571,-122.2766188,3a,75y,323.16h,87.15t/data=!3m7!1e1!3m5!1sZ7M37LTwRXIynelM8B9yag!2e0!6s%2F%2Fgeo1.ggpht.com%2Fcbk%3Fpanoid%3DZ7M37LTwRXIynelM8B9yag%26output%3Dthumbnail%26cb_client%3Dmaps_sv.tactile.gps%26thumb%3D2%26w%3D203%26h%3D100%26yaw%3D70.26178%26pitch%3D0%26thumbfov%3D100!7i16384!8i8192?hl=en
Dear Bio Rad
Thank you for this videos, is it possible to upload let say part 2 video to this one showing who to count the plates after incubation? However some people describe below who to count the plate, I do appreciated, but it will be more complete to add plate colonies counts video to this one!
many thanx to all
now i know what will i do to my bacteria. thanks for the info :) very well explained.
thanks for making & uploading such a good video.It's really help in understanding of rDNA Technology subject. I'm having an exam soon.
You count the number of colonies that grow there; that gives an estimation of the number of bacteria in the volume of liquid you inoculated the plate with.
To get the number of bacteria in 1 ml, you need to account for the volume you put on the plate and the dilution. If you put 0.1 ml on the plate, you need to times that by 10 to get it in ml. If you were counting 10^-5 dilution, you need to times it by 10^5 to get it in 10^1. Cancel those out and you have Colony forming units / ml.
how long should u wait for cooling down?
This way of dilutions are not supposed to be 10 minus 1, 10 minus 2, 10 minus 3 etc?
They are -1 -2- 3
Thank you so much for this video. It is very clear and easy to follow and understand.
Where can i buy these wicked neon green gloves??
Do you discard 100 microliters from the last (7th) microtube?
Thank you for uploading the video is quite educative.
Nice explanation, thanks for the video.
thanks for the explanation
hello! this video is very usefull..:) and it helps me so much for my upcoming research. can i ask.. what can i substitute to lb agar?
Phosphate buffer
Very informative , Thank you
Well explained!
very well explained
kumarsarvam singh
Subtitle please.
Nice video 👍🏻
Thank you sir
thank you
When you love greenery more than anyone !!
Totally rad-ical
there is no plaque counting in this video
ok
This isn't plaque assay for phage quantification
more of this videos on youtube and less justin bieber videos please :) thanks
why call it a plate count when you don't count the plates?
It didn't say how the colonies are counted
Alhamdulillah hadir
Then u didn't tell how to count the colonies
Hi, Sana! To count colonies, we recommend placing the plate lid-side down (so the agar is up and you're looking at agar underside) on a background that gives you good contrast between the agar and the colonies - for white colonies, a black background works well. Using a felt-tipped pen, dot each colony as you count; by dotting you'll know if you already counted a colony. If the plate has a lot of colonies, use the marker to draw lines on the backside of the plate to mark four equally-sized quadrants, count the colonies in one of the quadrants, and multiply by four for an estimated number of colonies on the plate.
❤❤❤❤❤❤
Sorry: how NOT who