Several people have commented about the amount of copper I used. Looking at things it seems that yes I had copied it over wrong and ended up with 10X the amount. This isn’t a big deal for spirulina since all the carbonate and high ph makes the copper only slightly soluble so it doesn’t really matter how much I add the concentration in Solution will be limited. It would however be toxic to other algae. I made this video on very little sleep so I guess I shouldn’t be surprised there were errors. I’m tempted to take the video down and redo it and also fix some other issues like not using a tripod. Thoughts?
I really enjoyed the video, and hope you dont take it down. Maybe if youre going to put the effort into a new video, just make a second instead? You could make it a part 2 where you correct the copper amount. Maybe discuss why you chose this algae too?
Leave this vidieo up, and if you get the chance to remake it with a tripod and some more sleep decide what you do with this vidieo at that time. Plenty of good info in this vidieo.
I mean…I vote yes simply because I like watching new videos from you. I already planned on rewatching this one about 100X before the next video came out. If your not happy with the product I would redo but I’ll watch anything you put out.
Used to watch you for YEARS all the time and hadn’t seen any of your stuff pop up in my sub box in over a year. Gonna be honest I kinda forgot about the channel. This video just popped up and I’m so glad I found you again man! Gonna have to put on notifications.
Cody’s lab and ethoslab are two top tier channels who are the pioneers of their field of content creation. True path makers, true mind twisters, true creativity and intelligence embodied as human beings. Nothing short of inspiration and aspiration.
The iron EDTA you're using is almost definitely iron (III), not (II). The chemical formula would suggest as much, and also the yellow/gold colour is classic for the ferric ion. Not sure how much that matters but just wanted to point out
Cody, quick question. If you're making 4 liters of growth media, wouldn't you need 80mL of the 50x bulk nutrient solution, rather than the 100mL you state in the video? Or was that a change made on purpose, just without informing us of why you'd want to end up with 5/4ths the bulk nutrients in the growth media?
Cody, How does the yield workout when going from gallons to weight of algae? Also, I was wondering why you are using plastic for the mixes. With the oxygen permeability of PET bottles. I would think that glass would be better. Also, if you are worried about the air that gets into the bottle every time you use some, here is an old idea. This comes from the darkroom comical storage. So many of those were very sensitive to oxidization. You would use glass marbles to bring the level back up to the starting level of the bottle. In your case, I think boiling the marbles would make sure that you do not introduce any bad actors into your algae colonies.
I would be interested in a comparison between your more precise method and a method more accessible to just people who want to give it a go (the simple vitamin method) and the differences in the growth results.
Really appreciate the great content. Keep up the great work. I know you work very hard, and may have downs In your life, but your my doing great. Hope everything is going well in your personal life and remember there is always someone in a worse situation.
Odd question what in milk makes it difficult to wash out of a cup? If you rinse a cup with milk in it I've noticed it can take multiple rinsings to get it clear vs something like sprite or coffee that washes out usually within 1 to two washes.
I'm not saying you're wrong about the Algaeside in miracle grow, But I recently opened a 5gal bucket I had sitting outside (for maybe a months time) filled with premixed miracle grow/water and it was filled with algae. So if its in there, its not working against the Louisiana sun lol.
It's definitely the first time I've ever heard about algaeside. The box specifically tells you not to use it near bodies of water or before rain because it will cause algea blooms. Is it some kind of low dose or for specific types of algea (algea refers to a lot of very different things, like entirely different kingdoms)? Why would they put stuff in there to kill microbes anyway? The microbes in soil are extremely important to soil health and are what make most of the nutrients bioavailable to plants?
I really hope you've got some better lab dishes by now. Ceramic, glass, and stainless steel will all hold your measured-out ingredients, they're all rigid so you can pick them up, and they're all washable and dishwasher safe so you can keep re-using them for decades.
I thought cyanobacteria is not actually true algae, and is actually specifically excluded from algae (regardless of it's common name of blue-green algae). Especially considering that cyanobacteria is prokaryotic and algae is eukaryotic.
Sometimes I forget how many crazy and unique projects you have going at the same time! That's why I love this channel, randomly hopping between mining and refining minerals to algae to a self-sustaining "mars habitat" prototype to mushrooms to bees...
@@tylerblackburn3760 funny enough, most of those projects we all love used to not be hobbies . You actually had to know this stuff to have a small farm or just sustain a family .
During my college days I was extremely into algae especially as a biofuel source. Seeing you experiment with algae makes me want to restart my own algae project.
I have a sealed bottle of algae in the attic. It has been alive for 3 years now and has been both frozen and almost boiled... It's still growing happily!
Your videos have never once failed to teach me things I likely never would've otherwise leaned, and somehow you've never left me uninterested. You've spurred curiosities in me which I've pursued into full-fledged projects, hobbies, and passions. You can post daily, or twice a year, I'll always be excited to see which rabbit hole you fall into next. Loved the video today Cody, never stop inspiring
I did a little research into growing spirulina after your algae panel video; in regards to the issue of algae dying from stepping up the media volume too fast, could it have something to do with the opacity of the solution protecting the algae against harmful UV light? when growing algae under artifical light, i saw some sources recommend starting with a lower light intensity immediately after propagation for this very reason. did a little more reading now, and apparently because of their structure "self-shading" is also a mechanism they use to protect themselves against excessive UV, ie the spirals can become tighter or looser coils to alter how much light is impacting them, and notably it takes some time for them to adapt. so basically you go from high density of algae causing a very opaque growth medium that shields against UV -> the algae adapt by loosening their coil to receive more light to compensate for the opacity -> you transfer them into a very LOW opacity solution where the UV immediately starts pummeling them and they die before they can adapt? just a theory!
I don't know a whole lot about algae (biology isn't my field), but if I had to take a wild guess at why the algae dislikes being diluted, maybe it's due to trace amounts of other bacteria? Where in a 'saturated' solution of algae, the algae dominate and cut off any foreign invasion at the knees, in a less 'full' environment, it's easier for other bacteria to build to large numbers and challenge the algae. Again, not my field, so I'm likely completely wrong on this. This is kinda an invitation for someone else who might have a better idea to share it.
Normally that wouldn't be a bad theory. But spirilina is grown in really high ph that actually kills most every other kind of bacteria. Which means its unlikely that a new bacteria takes over. The grow media presumptivly must change over time with the spirilina consuming many of the nutrients in solution, eventually untill are virtually depleted. I suspect that rapid osmotic pressure changes between the spirilina cell's inside and the grow medium are to blame for killing his spirilina when too much newly charged nutrient solution is added. In other words spirilina that have been sitting around awhile may need time to adjust to a significant quantity of new nutrients and salts.
@@kreynolds1123 Good theories, maybe the cells are also communicating and dislike the shock of suddlenly being seperated. For example bacteria also need some time to adjust from agar plates to liquid media and are added to a smaller volume first.
@@kreynolds1123 I don't think the pH of ~8-9 preferred by spirulina would kill "most" bacteria, there are plenty species it wouldn't. Also, it's an algae (an organism who's natural habitat is water) combined with all the salts he added I would think the osmotic pressure wouldn't be an issue. I would guess that it is a signaling molecule released by the algae that isn't present in high enough concentrations (in the diluted case) for the algae to continue its life cycle so it just chooses to die. Or as cody put it, they get lonely.
This is likely very true. He is not being very sterile, so he's relying too heavily on his algae outcompeting the contaminants. If he kept it fully sterile, he should be able to inoculate a new batch of media with very kittle algae
I've been curious as to what happened to the algae panel, it seems the panel didn't go quite to plan. Here's to hoping revision 2+ can take off and be a sustainable farm!
@@tartartar23 seemed to me like it was too much babysitting for too little return , this one as well . I think I'll try a plant , similar purpose . It grows fast af , is high protein , and would not require almost any babysitting .
Cody, I love that you want to keep the production a solo process, however I strongly recommend investing in a tripod so you have use of both of your hands 😂
Would you be able to add a typed up version of your ingredients and ratios either in the description or linked? It would be great to have a quick reference. Thanks!
@@ToTheGAMES Not that hard, but other people might want the list too, and if I just post it as a comment they may not see it. Could end up with a lot of people doing the same work.
Cody, I pretty much never comment on youtube videos, but I wanted to say that literally every time I see a new upload from you, my face instantly lights up with a smile. Always excited to see what you're working on, you've been an inspiration to me for quite some time! Edit: added in a missing word oops
I've heard that most of our crude oil is from marine algae. have you considered an experiment in cooking your algae under pressure to see if you can turn it into crude oil?
@@northernmetalworker i think the pressure is important so the low boiling volatiles don't just boil off having a condenser on the out put would capture most , there are probably other reflux and esterification reactions that go on over time as oil sits in the ground
@Cody'sLab as you know plants need Iron 2 and oxidized iron in this case is iron 3. EDTA, ethyldiaminetetracetic acid is perfect for keeping iron 2 safely where plants need it to be to access it unless you do something like bubbling air through the water. In the wild, plants live in the places where large amounts of Iron2 are available and thats in CO2 rich low disturbance water. The bubblers are what kills the algae in lower concentrations, the air has enough time to destroy the EDTA bond to the Iron2 making it into Iron3 again and starving the algae of photosynthesis. (the air pumps also cause biogenic decalcification further starving the algae) If you use an impeller pump to slowly circulate the water instead of air you will have more success keeping the nutrients available to the algae and therefore growing it in larger containers
@@theCodyReeder weirdly no it's the mechanical action of the bubbles forcing out co2 that breaks the bonds . If you can do it without disturbing the water the oxygen levels will saturate without breaking down the iron2 EDTA bond and keep it available to the algae for much longer . You could try looking up the dennerle aquarium system some of the documentation on it might give you a better understanding than I have . It's been a long time since I worked with it.
Hi Cody, I hope you're doing well. As a biochem student I love videos like these. I recently got to work with some cool proteins from Deinococcus radiodurans and immedeatly had to think about your radioactive projects and what cool stuff you would try if you ever got your hands on some of these amazing bacteria
Hi Cody, can you elaborate on the differences between chlorella and spirulina and why you opted to cultivate the latter? I was reading chlorella has bioavailable b12 in it which I find very useful.
The Morton's salt you used, was it iodized salt? If so, how much iodine would that add? (I honestly don't know how much iodine is in normal table iodized salt.) Would iodized salt be enough to throw off the trace amounts needed, or is the tolerance needed so wide that additional iodine from the salt doesn't really matter? Also, you may have mentioned it in a previous algae video, but why don't you use bubbler stones like what are used in aquariums for smaller air bubbles (more surface area of air, less splashing at the top)? Would the algae over time fill up the smaller holes in the bubbler stones, essentially blinding them over time? Or is it just not necessary so you didn't bother spending the money on aquarium bubbler stones?
@@theCodyReeder Re: Salt - I tried to get a good look at the label to see, but you had it turned so I could only just identify the brand. Thanx for the clarification. Re: air stones - That makes good sense.
The hesitation at 00:12:50 had me wondering what the heck he was going to say next. Then I realized he was desperately searching for a better term than Final Solution and couldn't.
For silica dioxide couldn't you just use extremely finely crushed quartz or sand? BTW, why didn't you show the kitty? I heard her meow but you didn't show her..
Quartz is crystalline silica and is much less soluble. Kind of like how a bunch of legos are harder to separate if they are stacked into a neat block opposed to being in a random pile. As for grinding it up it will still be blocks several hundred atoms across even at the nano scale, sure it would increase the surface area and would dissolve faster but you’d get the same rate from much larger pieces of amorphous silica. And the kitty is way too much chaos to have near chemicals. You might me able to hear them running around upstairs literally bouncing off the walls.
@@theCodyReeder I understand sodium or potassium silicate is not bioavailable? It readily dissolves in water and should be stable in alkaline solutions (or at least I would guess it should be)
Hi Cody, are you sure EDTA should be called an anti oxidant? From what I know EDTA is a ligand that is used to complex metals to allow them to stay in a water soluble complex rather than percipitating out. And the label of your Iron Chelate even states that it is an iron monosodium EDTA. Since EDTA has a 4- charge to it your iron has to be in a 3+ oxidation state.
14:44 That's how I feel about Azomite (A to Z of Minerals Including Trace Elements). What am I going to do with a 44lb bag (as it's sold locally). Azomite is mined close to you in Utah. It would certainly be cheaper for you compared to me buying it in Canada eh. High wonder how well Azomite would work for this type of algae application. It works wonders for growing grass.
I have had a chemistry dream since chemisty 3 in Highschool. I wanted to make a molecuole that combined as many elements as possible. IDEALY the entire periodic table of elements in ONE molecule. I think I only got to like 20 or so elements before I gave up. Would it be posible for you to attempt this? Excluding the radio active/man made elements at the bottum of the table of course...
I'm not sure if you'd be willing to do it, but it might be cool to do an experiment comparing algae which has had your nutrient solution and one with human multivitamins. Do you think there would be much difference in growth?
Some plants can not handle sudden changes either in the chemistry [things like the total Ph of the solution] or the actual solution fraction [an excess of pure water can make some plant cells swell up and explode, while to much salinity can dehydrate some plants to a fatal level], so its always best, where and when possible, to exactly match the Ph, salinity [all salts, not just NaCl] and total water saturation to keep the tiny plants from being shocked, poisoned or hyperinflated.
Fish are also highly sensitive to that sort of thing. I suppose natural bodies of water (even freshwater) tend to be pretty big so they don't change that quickly. maybe you could try submerging the galon of algae in the jar with a little hole in the lid in the 20 gal tank so the two bodies of water diffuse into each other very slowly. (kind of like how you sit the bag of fish in the fish tank)
You might like to try making a nano bubble generator to maximize gas absorption into the water. Take a graphite cylinder drill a hole in the center and glue an air hose to just inside the hole in the graphite cyliner. This is your new airstone. Place your new graphite airstone in a tube with water pumped through the tube. Water passing over the graphite airstone inside a tube is accelerated which rips off nano sized bubbles that stay submerged seemingly forever, maximizing gas absorption. It's so effective, that instead of bubbles that rise in a second or two, nanobubbles make the water look cloudy for minutes.
@@ChrisWijtmans Sounds bad. Why is that like adding asbestos? All the nano bubbles do is maximizes the gas exchange. They don't harm the spirilina in any way.
copper sulfate is an excellent fungicide. I noticed it on your table. I use it water tanks for sprayers to keep stuff from growing. Only takes a table spoon or 2 for a few thousand gallons of water. Does it change with all the other stuff?
That's what I was wondering too. He actually added it as one of the trace elements in the 1000x bottle. And it's what I thought was the algaecide in the other granulated mixtures you can get in the store. Makes no sense. If it's not copper sulfate, what exactly is the algaecide in the store-bought granulated mixtures?
Adding carbohydrates, like the filler in a ground-up multivitamin, is a great way to encourage mold growth and thereby spoil your algae culture. I know this form personal experience, unfortunately. Pretty awful smell. Also "chelate" is usually pronounced with a k sound. Nice video though. Thanks for sharing.
Several people have commented about the amount of copper I used. Looking at things it seems that yes I had copied it over wrong and ended up with 10X the amount. This isn’t a big deal for spirulina since all the carbonate and high ph makes the copper only slightly soluble so it doesn’t really matter how much I add the concentration in Solution will be limited. It would however be toxic to other algae.
I made this video on very little sleep so I guess I shouldn’t be surprised there were errors. I’m tempted to take the video down and redo it and also fix some other issues like not using a tripod. Thoughts?
Tripod!
I really enjoyed the video, and hope you dont take it down. Maybe if youre going to put the effort into a new video, just make a second instead? You could make it a part 2 where you correct the copper amount. Maybe discuss why you chose this algae too?
Leave this vidieo up, and if you get the chance to remake it with a tripod and some more sleep decide what you do with this vidieo at that time. Plenty of good info in this vidieo.
I mean…I vote yes simply because I like watching new videos from you. I already planned on rewatching this one about 100X before the next video came out. If your not happy with the product I would redo but I’ll watch anything you put out.
@@tyler7544 I second this. Better suggestion than mine. Then I can still watch this 100X while I wait for the redo lol
In this video Cody makes some really weird soup from his homeland.
Your health is low! Do you have any potions or food?
@@Opzombers There are new quest cards available at the guild for you.
Nice!
@@Opzombers zombie needs food badly
@@Opzombers actually I realize you're talking about something else think fable?
Cody, this is why I like you! One week you are prospecting, another refining minerals and the next making algae!
A Cody's Lab video?? What a happy surprise, my night just got a whole lot better!
I wonder if Cody has ever thought of just going full breaking bad style chemist.
Of course he's thought of it. The question is whether he's actually done it.
@@vigilantcosmicpenguin8721 the government raided his place after the yellowcake video and he didn't get charged with anything. He's clean.
@@GigsVT As if they’d ever find his secret underground lair.
Used to watch you for YEARS all the time and hadn’t seen any of your stuff pop up in my sub box in over a year. Gonna be honest I kinda forgot about the channel. This video just popped up and I’m so glad I found you again man! Gonna have to put on notifications.
You and EthoSlab are such an inspiration!
Cody’s lab and ethoslab are two top tier channels who are the pioneers of their field of content creation. True path makers, true mind twisters, true creativity and intelligence embodied as human beings. Nothing short of inspiration and aspiration.
I wonder if the Slabs are the same person 🤔
Can't wait to add this to my neighbor's pool! Thanks Cody!
Your videos push me to use my brain. thx.
Great video. Really looking forward to the next video in this series
Cloud chamber next please!! CodysLab style!
I don't know a whole lot about algae cultures and ionized minerals, but I do like the pretty colors.
One handed chemistry is an art.
Im excited to see this new panel!
Maybe you can resell the molybdenum as 1lb minus a few gram :P
Nine-hundred and ninety-six grams;
you need four more
that full metal alchemist watch
YEEEEESSSSSSS! HELL YES!
Stoked to see a codys lab video in my feed.
I love the green of these and think it would look great in my house.
Cody's on his side of the mountain!!
This reminded me fullmetal alchemist, collecting the elements the human is made of
The iron EDTA you're using is almost definitely iron (III), not (II). The chemical formula would suggest as much, and also the yellow/gold colour is classic for the ferric ion. Not sure how much that matters but just wanted to point out
Cody really made the chug jug
Love the Alchemist watch!
I get the feeling that figuring out what ratios of chemicals to use from the ratios of elements may be NP-complete...
Love your channel man
what a roundabout way to find an excuse to eat those silica gel packets
Someone buy this man a tripod!!!!
Only having 1 hand to work with seems challenging and almost unnecessary if he just had a tripod...
This terrifies me. I would be so afraid that I’d poison myself. How did you figure this out and have you noticed any health benefits or problems?
Cody lives without fear.
Cody, quick question.
If you're making 4 liters of growth media, wouldn't you need 80mL of the 50x bulk nutrient solution, rather than the 100mL you state in the video? Or was that a change made on purpose, just without informing us of why you'd want to end up with 5/4ths the bulk nutrients in the growth media?
Honestly, sometimes I wish Cody was my Chemistry Teacher.
Maybe it dies because it just gets lonely...Cody is such a romantic.
Anybody else looking forward to the day that Cody goes full Breaking Bad??? 🧐🤷🏻♂️
What’s My Name!!! 😡
Cody!
You’re Damn Right it is! 😤
🤣🤣🤣
Cody, How does the yield workout when going from gallons to weight of algae? Also, I was wondering why you are using plastic for the mixes. With the oxygen permeability of PET bottles. I would think that glass would be better. Also, if you are worried about the air that gets into the bottle every time you use some, here is an old idea. This comes from the darkroom comical storage. So many of those were very sensitive to oxidization. You would use glass marbles to bring the level back up to the starting level of the bottle. In your case, I think boiling the marbles would make sure that you do not introduce any bad actors into your algae colonies.
I would be interested in a comparison between your more precise method and a method more accessible to just people who want to give it a go (the simple vitamin method) and the differences in the growth results.
If you boil the solution you could also use simple soda instead of baking soda - a little bit cheaper ;-)
Really appreciate the great content. Keep up the great work. I know you work very hard, and may have downs In your life, but your my doing great. Hope everything is going well in your personal life and remember there is always someone in a worse situation.
This goes into the............ Final solution. Hmmmm Cody
Looks delicious
"Soylent Green"
This is how it begins ;)
Ah, but can you pronounce the full name of EDTA?
Nice to see chemicals I recognise from work being used, though.
If you were to tell me Trace element solution was any color but blue raspberry blue......
THAT VIDEO WAS 20 MINUTES LONG? THAT FELT LIKE 10
Odd question what in milk makes it difficult to wash out of a cup? If you rinse a cup with milk in it I've noticed it can take multiple rinsings to get it clear vs something like sprite or coffee that washes out usually within 1 to two washes.
Hey cody. Off topic but I noticed what ya have there near the scale.
If you were a state alchemist, what would your nickname be? Has to be original.
ah yes the shrek blood jars
I'm not saying you're wrong about the Algaeside in miracle grow, But I recently opened a 5gal bucket I had sitting outside (for maybe a months time) filled with premixed miracle grow/water and it was filled with algae. So if its in there, its not working against the Louisiana sun lol.
It might biodegrade over time. 🤔
It's definitely the first time I've ever heard about algaeside. The box specifically tells you not to use it near bodies of water or before rain because it will cause algea blooms. Is it some kind of low dose or for specific types of algea (algea refers to a lot of very different things, like entirely different kingdoms)?
Why would they put stuff in there to kill microbes anyway? The microbes in soil are extremely important to soil health and are what make most of the nutrients bioavailable to plants?
I really hope you've got some better lab dishes by now. Ceramic, glass, and stainless steel will all hold your measured-out ingredients, they're all rigid so you can pick them up, and they're all washable and dishwasher safe so you can keep re-using them for decades.
hi cody, i like your videos.
I thought cyanobacteria is not actually true algae, and is actually specifically excluded from algae (regardless of it's common name of blue-green algae). Especially considering that cyanobacteria is prokaryotic and algae is eukaryotic.
8:49 perfect!)
Is a crushed multi vitamin pill per pound of algae equivalent to a gallon of water?
Could you take plant food and cook away or otherwise destroy the algicides?
Be careful, I heard the Elric brothers tried this and ended up losing some body parts.
Cody I'm always wondering do you have chemicals PhD or something like that?
shouldn't it have been 80ml of concentrate or doesn't 25% extra make much of a difference?
Did you test using just micronized rock dust?
I wander how you would recover if the media got polluted? Or start smelling funny...? Increase the ph?
What happened to that solar panel idea of yours? Was it not working out? P
i did enjoy
Final solution 👽
love the vids
what is this algae culture for?
I wonder who would use that much of the molybdenum salt... Seems weird to be able to buy it in such large amounts
Sometimes I forget how many crazy and unique projects you have going at the same time!
That's why I love this channel, randomly hopping between mining and refining minerals to algae to a self-sustaining "mars habitat" prototype to mushrooms to bees...
For nerds with adhd and too many hobbies
And all of it just happens naturally. Cody doesn't need to come up with crazy challenges or anything, because he just does what he wants.
@@tylerblackburn3760 funny enough, most of those projects we all love used to not be hobbies . You actually had to know this stuff to have a small farm or just sustain a family .
@@alanomofo he the bloke you need if ever get stranded on driest island or something with no out side help?
@@dh2032 exactly! I want a cody on my side in case of the apocalypse.
During my college days I was extremely into algae especially as a biofuel source. Seeing you experiment with algae makes me want to restart my own algae project.
I also dove into algea quite deeply, but for eating sustainably... Its a dam interesting and has tons of potential
Do it friend, double dog dare you
I have a sealed bottle of algae in the attic. It has been alive for 3 years now and has been both frozen and almost boiled... It's still growing happily!
@@runed0s86 that is some tough algae.
Your videos have never once failed to teach me things I likely never would've otherwise leaned, and somehow you've never left me uninterested. You've spurred curiosities in me which I've pursued into full-fledged projects, hobbies, and passions. You can post daily, or twice a year, I'll always be excited to see which rabbit hole you fall into next. Loved the video today Cody, never stop inspiring
I did a little research into growing spirulina after your algae panel video; in regards to the issue of algae dying from stepping up the media volume too fast, could it have something to do with the opacity of the solution protecting the algae against harmful UV light? when growing algae under artifical light, i saw some sources recommend starting with a lower light intensity immediately after propagation for this very reason. did a little more reading now, and apparently because of their structure "self-shading" is also a mechanism they use to protect themselves against excessive UV, ie the spirals can become tighter or looser coils to alter how much light is impacting them, and notably it takes some time for them to adapt. so basically you go from high density of algae causing a very opaque growth medium that shields against UV -> the algae adapt by loosening their coil to receive more light to compensate for the opacity -> you transfer them into a very LOW opacity solution where the UV immediately starts pummeling them and they die before they can adapt? just a theory!
Thank you for the information!
I'm loving the algae stuff. Hope you're well Cody!
Isn't any video with algae in it "algae growing media"?
I guess it's algae growing multimedia.
Out of curiosity, what made you go with zarrouk's media instead of the simpler soilwater media or UTEX's Spirulina media?
It was just what I found first. 😂
@@theCodyReeder I like this mentality. Too many people turn perfection into the enemy of good
@@theCodyReeder ah yes, laziness, the father of invention :p
@@defenestrated23 Is it laziness? We can only take in so much. If you use what you think you have too, that’s only being not totally expert. Not lazy.
@@theCodyReeder Spoken like a true scientist
I don't know a whole lot about algae (biology isn't my field), but if I had to take a wild guess at why the algae dislikes being diluted, maybe it's due to trace amounts of other bacteria? Where in a 'saturated' solution of algae, the algae dominate and cut off any foreign invasion at the knees, in a less 'full' environment, it's easier for other bacteria to build to large numbers and challenge the algae. Again, not my field, so I'm likely completely wrong on this. This is kinda an invitation for someone else who might have a better idea to share it.
Normally that wouldn't be a bad theory. But spirilina is grown in really high ph that actually kills most every other kind of bacteria. Which means its unlikely that a new bacteria takes over.
The grow media presumptivly must change over time with the spirilina consuming many of the nutrients in solution, eventually untill are virtually depleted. I suspect that rapid osmotic pressure changes between the spirilina cell's inside and the grow medium are to blame for killing his spirilina when too much newly charged nutrient solution is added. In other words spirilina that have been sitting around awhile may need time to adjust to a significant quantity of new nutrients and salts.
@@kreynolds1123 Good theories, maybe the cells are also communicating and dislike the shock of suddlenly being seperated. For example bacteria also need some time to adjust from agar plates to liquid media and are added to a smaller volume first.
@@kreynolds1123 I don't think the pH of ~8-9 preferred by spirulina would kill "most" bacteria, there are plenty species it wouldn't. Also, it's an algae (an organism who's natural habitat is water) combined with all the salts he added I would think the osmotic pressure wouldn't be an issue. I would guess that it is a signaling molecule released by the algae that isn't present in high enough concentrations (in the diluted case) for the algae to continue its life cycle so it just chooses to die. Or as cody put it, they get lonely.
Maybe Cody is right and when diluted too much the bacteria gets lonely. They cannot take cab or jump on train in order to visit far away soulmate.
This is likely very true.
He is not being very sterile, so he's relying too heavily on his algae outcompeting the contaminants. If he kept it fully sterile, he should be able to inoculate a new batch of media with very kittle algae
I've been curious as to what happened to the algae panel, it seems the panel didn't go quite to plan. Here's to hoping revision 2+ can take off and be a sustainable farm!
You're talking about the diy plastic bottle thingy he did some while ago ?
@@yasirrakhurrafat1142 ye
@@tartartar23 seemed to me like it was too much babysitting for too little return , this one as well .
I think I'll try a plant , similar purpose .
It grows fast af , is high protein , and would not require almost any babysitting .
Cody, I love that you want to keep the production a solo process, however I strongly recommend investing in a tripod so you have use of both of your hands 😂
Maybe RoboCody could hold the camera?
No! Shaky cam is a staple!
the worst thing is that he has tripods, tbh I usually don't make it through those videos even if it is interesting.
Noo! Never
Would you be able to add a typed up version of your ingredients and ratios either in the description or linked? It would be great to have a quick reference. Thanks!
2 hours ago?
@@emperorstorm3266 could be a patreon/member benefit that they get the link before it goes public
@E Van The youtubers themselves decide if they want a video to be premiered or not
How hard is it to pause the video at the list and type it over? :')
@@ToTheGAMES Not that hard, but other people might want the list too, and if I just post it as a comment they may not see it. Could end up with a lot of people doing the same work.
Cody, I pretty much never comment on youtube videos, but I wanted to say that literally every time I see a new upload from you, my face instantly lights up with a smile. Always excited to see what you're working on, you've been an inspiration to me for quite some time!
Edit: added in a missing word oops
I've heard that most of our crude oil is from marine algae.
have you considered an experiment in cooking your algae under pressure to see if you can turn it into crude oil?
He has already made oil from algae, but he just heated the algae up in a test tube to make it.
I’m pretty sure he’s done that
@@lucaslac124 I've read that pressure is a factor in proper production.
but I'll look up his video for sure, thanks.
@@northernmetalworker i think the pressure is important so the low boiling volatiles don't just boil off having a condenser on the out put would capture most , there are probably other reflux and esterification reactions that go on over time as oil sits in the ground
@@lucaslac124 i think it's a different oil
You are the best Cody. I hope the algae grows and becomes sentient one day, and then takes you as their king.
@Cody'sLab as you know plants need Iron 2 and oxidized iron in this case is iron 3. EDTA, ethyldiaminetetracetic acid is perfect for keeping iron 2 safely where plants need it to be to access it unless you do something like bubbling air through the water. In the wild, plants live in the places where large amounts of Iron2 are available and thats in CO2 rich low disturbance water.
The bubblers are what kills the algae in lower concentrations, the air has enough time to destroy the EDTA bond to the Iron2 making it into Iron3 again and starving the algae of photosynthesis. (the air pumps also cause biogenic decalcification further starving the algae)
If you use an impeller pump to slowly circulate the water instead of air you will have more success keeping the nutrients available to the algae and therefore growing it in larger containers
Interesting. Is it just the overwhelming amount of oxygen?
@@theCodyReeder weirdly no it's the mechanical action of the bubbles forcing out co2 that breaks the bonds . If you can do it without disturbing the water the oxygen levels will saturate without breaking down the iron2 EDTA bond and keep it available to the algae for much longer . You could try looking up the dennerle aquarium system some of the documentation on it might give you a better understanding than I have . It's been a long time since I worked with it.
I was thinking the same about the bubbles. It can kill the algae. The bubbles only work when its high CO2 in closed loop i believe.
Hi Cody,
I hope you're doing well. As a biochem student I love videos like these. I recently got to work with some cool proteins from Deinococcus radiodurans and immedeatly had to think about your radioactive projects and what cool stuff you would try if you ever got your hands on some of these amazing bacteria
I woud love to see some of that in culture and compare it to radiation tolerant mycelium
Yay! NEW Cody video!
cody have you considered mounting a camera onto your chest or your head to make filming easier from the first person perspective?
Hi Cody, can you elaborate on the differences between chlorella and spirulina and why you opted to cultivate the latter? I was reading chlorella has bioavailable b12 in it which I find very useful.
Upvoting this comment
Drop everything new Cody's lab
In secret, Cody is actually just a collection of algae, and this is just his dinner.
The Morton's salt you used, was it iodized salt? If so, how much iodine would that add? (I honestly don't know how much iodine is in normal table iodized salt.) Would iodized salt be enough to throw off the trace amounts needed, or is the tolerance needed so wide that additional iodine from the salt doesn't really matter?
Also, you may have mentioned it in a previous algae video, but why don't you use bubbler stones like what are used in aquariums for smaller air bubbles (more surface area of air, less splashing at the top)? Would the algae over time fill up the smaller holes in the bubbler stones, essentially blinding them over time? Or is it just not necessary so you didn't bother spending the money on aquarium bubbler stones?
If the goal is to oxygenate the water, then surface agitation is a thousand times better than small bubbles
The salt was not iodized. In my opinion air stones are just another hard to clean surface to grow harmful bacteria.
@@dylanzrim3635 OK. I did not know that. Thanx.
@@theCodyReeder Re: Salt - I tried to get a good look at the label to see, but you had it turned so I could only just identify the brand. Thanx for the clarification. Re: air stones - That makes good sense.
Why did you choose to use Spirulina rather than a different algae like Chlorella?
Miss you Cody! Always nice to see a new video
The hesitation at 00:12:50 had me wondering what the heck he was going to say next. Then I realized he was desperately searching for a better term than Final Solution and couldn't.
For silica dioxide couldn't you just use extremely finely crushed quartz or sand? BTW, why didn't you show the kitty? I heard her meow but you didn't show her..
Quartz is crystalline silica and is much less soluble. Kind of like how a bunch of legos are harder to separate if they are stacked into a neat block opposed to being in a random pile. As for grinding it up it will still be blocks several hundred atoms across even at the nano scale, sure it would increase the surface area and would dissolve faster but you’d get the same rate from much larger pieces of amorphous silica.
And the kitty is way too much chaos to have near chemicals. You might me able to hear them running around upstairs literally bouncing off the walls.
@@theCodyReeder I understand sodium or potassium silicate is not bioavailable? It readily dissolves in water and should be stable in alkaline solutions (or at least I would guess it should be)
Algae is the best pet you could have.
Hi Cody, are you sure EDTA should be called an anti oxidant? From what I know EDTA is a ligand that is used to complex metals to allow them to stay in a water soluble complex rather than percipitating out. And the label of your Iron Chelate even states that it is an iron monosodium EDTA. Since EDTA has a 4- charge to it your iron has to be in a 3+ oxidation state.
EDTA is a chelating agent... Holds the Iron like a crab ...It should not be thought as an oxidising agent.
14:44 That's how I feel about Azomite (A to Z of Minerals Including Trace Elements). What am I going to do with a 44lb bag (as it's sold locally). Azomite is mined close to you in Utah. It would certainly be cheaper for you compared to me buying it in Canada eh. High wonder how well Azomite would work for this type of algae application. It works wonders for growing grass.
The copper sulfate surprised me as it is a good algecide.
I have had a chemistry dream since chemisty 3 in Highschool. I wanted to make a molecuole that combined as many elements as possible. IDEALY the entire periodic table of elements in ONE molecule. I think I only got to like 20 or so elements before I gave up. Would it be posible for you to attempt this? Excluding the radio active/man made elements at the bottum of the table of course...
I knew from experience as soon as you put that silica gel in the mortar that we were going to have a problem. 😅
I'm not sure if you'd be willing to do it, but it might be cool to do an experiment comparing algae which has had your nutrient solution and one with human multivitamins. Do you think there would be much difference in growth?
Some plants can not handle sudden changes either in the chemistry [things like the total Ph of the solution] or the actual solution fraction [an excess of pure water can make some plant cells swell up and explode, while to much salinity can dehydrate some plants to a fatal level], so its always best, where and when possible, to exactly match the Ph, salinity [all salts, not just NaCl] and total water saturation to keep the tiny plants from being shocked, poisoned or hyperinflated.
Fish are also highly sensitive to that sort of thing. I suppose natural bodies of water (even freshwater) tend to be pretty big so they don't change that quickly. maybe you could try submerging the galon of algae in the jar with a little hole in the lid in the 20 gal tank so the two bodies of water diffuse into each other very slowly. (kind of like how you sit the bag of fish in the fish tank)
@@charleslambert3368 Diffusion and acclimation definitely would make more sense.
You might like to try making a nano bubble generator to maximize gas absorption into the water.
Take a graphite cylinder drill a hole in the center and glue an air hose to just inside the hole in the graphite cyliner. This is your new airstone. Place your new graphite airstone in a tube with water pumped through the tube.
Water passing over the graphite airstone inside a tube is accelerated which rips off nano sized bubbles that stay submerged seemingly forever, maximizing gas absorption.
It's so effective, that instead of bubbles that rise in a second or two, nanobubbles make the water look cloudy for minutes.
that would be like adding asbestos to his nutrient solution.
@@ChrisWijtmans Sounds bad. Why is that like adding asbestos? All the nano bubbles do is maximizes the gas exchange. They don't harm the spirilina in any way.
So *this* is what all the mad scientists have bubbling in the background of all their labs: eco friendly food sources!
Careful, Cody, remember the Law of Equivalent Exchange! After all, what could equal the value of a human soul?
copper sulfate is an excellent fungicide. I noticed it on your table. I use it water tanks for sprayers to keep stuff from growing. Only takes a table spoon or 2 for a few thousand gallons of water. Does it change with all the other stuff?
That's what I was wondering too. He actually added it as one of the trace elements in the 1000x bottle. And it's what I thought was the algaecide in the other granulated mixtures you can get in the store. Makes no sense. If it's not copper sulfate, what exactly is the algaecide in the store-bought granulated mixtures?
@@dansw0rkshop Yes Copper sulphate is a fungicide.. but you have to take concentrations into account... here he is using trace amount
Adding carbohydrates, like the filler in a ground-up multivitamin, is a great way to encourage mold growth and thereby spoil your algae culture. I know this form personal experience, unfortunately. Pretty awful smell. Also "chelate" is usually pronounced with a k sound.
Nice video though. Thanks for sharing.
Yeah the vitamins are not ideal but I have used them with success.