This video explains how protein fold in vivo and inclusion bodies refold procedure. It also introduces IBs refolding methods, such as dilution and chromatography.
Fantastic video about the Refolding of Inclusion Body Proteins from E Coli, which is the main subject of our research. We also want to learn more the detailed information about its processes.
Fantastic video about the Refolding of Inclusion Body Proteins from E Coli, which is the main subject of our research. We also want to learn more the detailed information about its processes.
Is it necessary to establish efficient refolding protocols?
The protocols for dilution or chromatography methods are commonly used. The most important is the buffer and other conditions you used in the process.
What refolding method do you suggest for cost effective purpose?
Dilution is the cheaper way, but the effect is not as good as chromatography. And the subsequent concentration is also laborious.
Do you have general protocols for protein refolding?
Unfortunately, no. The process is similar for different proteins, however, the condition details various greatly.
However, now the refolding of inclusion bodies has become a bottleneck in biopharmaceutical.
Auto voice is creepy, as if she's going to refold me.. left after a minute
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