Home DNA extraction

How can you isolate DNA in your own kitchen?
Reagents and equipment: banana, 200 mL isopropyl alcohol, 1 tbsp table salt, 10 drops of liquid soap containing sodium dodecyl sulfate, 100 mL hot water, 100 mL cold water, shot glass, blender, glasses, gauze, colander, deep dish.
Prepare a precipitating solution: combine 1 tbsp table salt and 10 drops of liquid soap containing sodium dodecyl sulfate in a glass. Add 100 mL hot water. Stir well.
Place a peeled banana in a glass. Add 5 tablespoons of precipitating solution and 100 mL cold water. Blend the mixture thoroughly.
Filter the puree through several layers of gauze. Carefully add 200 mL ice-cold isopropyl alcohol by pouring the liquid down the side of the glass. Observe the formation of a white mass in the alcohol layer.
DNA is a molecule with a complex structure that stores and passes the genetic information of every living organism on Earth from generation to generation. This simple method for DNA extraction is based on the following principles: the liquid soap contains surfactants, which help destroy the cell membranes and nuclei, while sodium ions from the salt bind to the phosphate groups of the DNA molecules, helping isolate the DNA from the solution. Adding cold isopropyl alcohol reduces the resulting DNA and sodium compound solubility in water. You can also use this method to isolate the DNA of strawberries, peaches, and tomatoes.
Safety precautions: conduct this experiment only under adult supervision.

[@ABC-cw7zv]

Lol the first time I did this it was by rubbing a q-tip on my gums for a minute. I had a nice little tube of my dna. It was very visible and exactly how this video showed it. That was in 2005. Science never gets boring.

[@sleepy4205]

Did u do the same experiment like the video did?

[@AE-pb3yv]

REALLY NEAT!!

[@ramakantgupta4273]

make a video on rna extraction

[@artcruiser]

If I use 70% isopropyl alcohol will my DNA still come?? Also my DNA didn't come out as a cluster like in the video, when I took mine out it was kind of slimy and not a cluster it was more of a round shape is that okay?? Please reply . Thank You!!

[@3lwad]

Thank you so much

[@oleiva]

OK, and how can I read it?

[@aamishra4117]

Awesome experiment. Make more videos please

[@sankaseri622]

Really cool

[@drshoaibsheeno]

Wow nice

[@harikrishnanhk8558]

Is there any calculations for this ?

[@Qalb-E-Momin.]

I tried with peas and I got it !

[@harikrishnanhk8558]

Awesome

[@anmolgaur686]

Keep it up 👍👍👍👌 come up with protein quantification and RNA isolation.....

[@abhishekmotivational2920]

Nice

[@remiblache4222]

cool!!!!!!

[@mifthahulhuda8144]

Magnificent!

[@setusof]

Can we use ethenol instead of isopropyl alcohol pleas solve my problem I don't have isopropyl alcohol

[@MELScience1]

It is also called Rubbing alcohol, you may actually have those 👨‍🔬👩‍🔬 But you may try it with ethanol too.

[@divyaa4459]

can we use nailpolish remover for alcohol?

[@diliniperera6842]

Yes

[@timmytheimpaler1750]

Now... how do i use this

[@sebastiancastaneda7284]

Make the experiment with a human and use him like the banana

[@samofficial640]

How can you say that it is DNA? Can't it be other component?

[@cynv2808]

How can you prove it's really DNA? we did this experiment at school but there's a lot of skepticism...

[@MELScience1]

This sim­ple method for DNA ex­trac­tion is based on the fol­low­ing prin­ci­ples: the liq­uid soap con­tains sur­fac­tants, which help de­stroy the cell mem­branes and nu­clei, while sodi­um ions from the salt bind to the phos­phate groups of the DNA mol­e­cules, help­ing iso­late the DNA from the so­lu­tion. Adding cold iso­propyl al­co­hol re­duces the re­sult­ing DNA and sodi­um com­pound sol­u­bil­i­ty in wa­ter. You can also use this method to iso­late the DNA of straw­ber­ries, peach­es, and toma­toes.

[@memesbymicah782]

Yea that’s right

[@ach.mathieu]

You can prove that dna easily but you should have materiel you will do an electrophoresis or use an enzyme DNA ase that destroy dna if its dna it will be destroyed but I should notice that this result is not purely dna

[@sweet-ks7fq]

Can we use. 70 percent Isopropyl alcohol

[@bobbieultee5015]

Yes, you can use it as well. Just used it in my lab experiment.

[@raihanhasbilla6430]

Nile red
Do this first.......
But, this is easier......

[@roshanikarunarathne976]

Do we can use this method for isolate dna from plant leaves

[@djaafarnedder7778]

hello thank you so much ... i just wanna know if there's a method to extract the yeast DNA at home
the yeast we use to make bread !! . thank you

[@MELScience1]

Glad that you liked it :) We cannot confirm this one is suitable for yeast but you may try! Also just try to look it up the internet, maybe you'll find a solution ;) Merry Christmas!🎄

[@djaafarnedder7778]

@@MELScience1 thank you so much, i'll try and give you a feed back later Merry Christmas ✨✨

[@Lislio]

@@djaafarnedder7778 you would need quite a lot of yeast for this to work, but when the yeast is in the soap solution, you could freeze it completely overnight, then warm it up to room temperature, then continue like the rest if the video

[@anusha7631]

@@Lislio hey, at what temperature should we freeze the yeast like normal freezer is fine?

[@Lislio]

@@anusha7631 -20C would be great, but if your freezer isnt that cold it will probably work still

[@alvindoakerta4417]

Can i use this method for fish DNA?
Please answer me

[@drlove3825]

*Great video.very simple method.can I use it for hair DNA extraction?*

[@MELScience1]

Needs researching :)

[@krasivayademie]

Can i try this with my saliva?

[@crystalwu5950]

Would the same procedure work with blueberries, raspberries and blackberries?

[@MELScience1]

Yep, just mash them nicely :)

[@crystalwu5950]

@@MELScience1 OK，thank you!

[@divyagulati714]

Basically, v can do it using any fruit ryt ???

[@crystalwu5950]

@@divyagulati714 I'm pretty sure yes, but the amounts of DNA would differ.

[@divyagulati714]

@@crystalwu5950
Okay
Thank you 😇

[@akosiratbu6929]

Does this work when I put some saliva on it? Because I know that saliva has Dna

[@MELScience1]

Unfortunately not, it only works with some of the fruits.

[@Lislio]

It can work, but youll need alot of saliva, as it has a lot less dna

[@pg2116]

What about human blood

[@ranjiniranju6891]

It works

[@scienceathome3482]

Can we take soap gel with out sodium dodecyl sulphate in it???

[@MELScience1]

It will not work without sodium dodecyl sulphate. It is also called sodium lauryl sulfate, pretty common ingredient in soap 👨‍🔬👩‍🔬

[@liyadsalem192]

My friends used PCR

[@Frepzter]

Can i use ethanol hand sanitizer?

[@allenhuseinovic9994]

yes

[@scienceexplore9534]

We can also Use Nailpaint Remover instead of Alcohol...
You can Watch my video, I've done the same!😊

[@iknowtom]

What could I do? Like what will that do for me

[@MELScience1]

Just don't eat/drink it afterwards :)

[@rudraaradhya8022]

From GIS

[@amaanshaikh9055]

What about human dna?

[@MELScience1]

Needs researching 👨‍🔬👩‍🔬

[@bogdanghilencea4533]

Does it work with ethanol too?

[@Lislio]

Yes it does

[@anaisphotographie1730]

Quatrième

[@aakashfighter1407]

Can put our own blood in this solution for extracting our dna

[@MELScience1]

you can try :)

[@gamerpros413]

yeet?

[@eunaekim9216]

Before you start this process, please think about it - are you okay with essentially wasting fruit?

[@MELScience1]

A sacrifice to science :)

[@alligator985]

How is it wasted? Because it wasn’t eaten? It had a different purpose, education! It wasn’t wasted