1) PCR (Polymerase Chain Reaction) Tutorial - An Introduction
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- เผยแพร่เมื่อ 29 ก.ย. 2024
- This PCR introduction will demonstrate that PCR is a fundamental technique used to amplify fragments of DNA, frequently using the Taq polymerase to facilitate the amplification during the thermal cycling process.
There are many variations of the PCR process including RT PCR for reverse transcription from an RNA template, qPCR for quantitative real time PCR to quantify the amplification during the chain reaction, and many more techniques to optimize the process.
In this PCR video animation, we will give you an overview of the polymerase chain reaction steps as well as the role Taq DNA Polymerase plays in the PCR process. You will learn about PCR applications and factors important in the design of your PCR experiment.
For more information on Polymerase Chain Reaction (PCR) and for a list of the sources used, please visit:
➜ Knowledge Base: info.abmgood.c...
Thank you to all of the amazing community contributions towards translating this video into other languages including:
➜ Indonesian
(To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.)
Check out our other video series:
➜ Polymerase Chain Reaction (PCR) - An Introduction: • 1) PCR (Polymerase Cha...
➜ CRISPR Cas9: • CRISPR Cas9 - A Brief ...
➜ Adeno Associated Virus: • 1) Adeno Associated Vi...
➜ Cell Culture: • Cell Culture
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Ditching school and learning from TH-cam
Thanks for watching! :)
Why all my professors failed to explain simply like this?
Thanks a lot
Hey Anh, thanks for leaving a comment! Glad to hear that the video was helpful :)
Well, that background music is going to be stuck in my head all day lol
yall may be here from biology but im here from forensic science ;)
Hi, the lecture is quite nice and very clear thank you for the excellent PCR tutorial link.
thanks a lot! this video is quite helpful the picture is vivid. Hopefully, more and more great instruments will be invented by your company.
Thanks for watching! We are really glad to hear that you found this video helpful. More interesting videos are coming so stay tuned!
amazing! i'm a student from Korea, and I'd like to watch this video in Korean, too. i'll very thank you if you try to make this great video about pcr in multiple languages. XD
Hi there! Thanks for watching and leaving such a nice comment! Yes, currently our videos are only officially translated into english and mandarin. However, youtube has an automatic translation of captions (if you click on the gear icon in the bottom right corner of the video > click "subtitle" > click "autotranslate" > select your language) which you might find helpful!
Great video. Thank you
Thank you Nathan, glad you enjoyed it!
Really great presentation . A huge thumb up from Egypt :D
Very well animated video! It makes everything much easier to understand. Thank you for posting this!
Hello Alyssa,
Thanks for watching! Glad to hear that you found the video helpful! :)
Thanks for the extra notes....quality control points...priceless...Hokudai
Thanks for watching, Keneth!
Thank You, it is really useful
You're very welcome, and thanks for your comment!
I like your unique style of teaching.
Nice and detailed explanation. Thanks from Bangladesh 🇧🇩
You are most welcome :)
TH-cam is becoming a teaching tool more advanced than previous
Great video , many thanks 😅
You're welcome, Marwa. Thanks for the nice comment!
Hi Applied Biological Materials
Thank you for this great video. I'm from Indonesia
This was very helpful. Thank you!
You're welcome, Zay! Glad to hear that the video was helpful.
Question: how can we know if a sample is more rich in GC or in AT?
Hello Aliah,
There are tools such as a GC content calculator to analyze the % in a given template sequence. Simply Google "GC Content Calculator" and paste your template sequence into the tool to find out the percentage.
Awesome video tutorial!
Hi Buhari! Thanks for the comments and glad you enjoyed our video. Let us know if you have any questions - we'd be happy to help!
link error: 404 not found
this video is everything
Number 15
Polymerase Chain Reaction
very helpful, thanks
Hi Haley, thanks for leaving a comment! Let us know if you have any questions!
Why don't you show actual video of all of this?
Great explanation!
Hi Koem, thanks for watching and leaving a comment! Glad you enjoyed our video - please let us know if you have any questions and we'd be happy to help answer them.
amazing presentation
Hi Deepthi, thanks for your comment and watching our video! If you want to learn more about PCR, we do have a knowledge base that explores it in more depth: goo.gl/OAuLRV
Great video but please remove background music, very distracting!
Hello there,
We are well aware of this issue but unfortunately we couldn't replace all the old videos.
However, we have made improvements on our latest videos so please check them out! :)
As an alternative solution, you can always mute the video and
turn the caption on if the background music bothers too much :)
Thank you!
fantestic video
Hi Waqas, thanks for watching! Let us know if you have any questions!
great information and presentation, still - audio background sucks
Thanks so much for your input!
THis is my first clear understanding to PCR Am very impressed Thanks a bunch
You are welcome :D
lit.
Thank you for the video....
You're welcome :) Hope you found it helpful!
I wonder what’s the name of the music track in the background. So funny!
Hi, it should be a track from TH-cam's audio library.
awesome video .
Hi Shivam, thanks for your comment! Glad you enjoyed the video!
Thanks. I'm from Indonesia
Thanks for watching!
Bisa saya kontak?
@@dxlabs iyaa ada apa pak?
nice vedio,,, but background music is effective
Hello Hifza,
Thanks so much for your feedback! We have made improvements on our recent videos. Please check them out too ;)
Just came beacause of module
Thank you so much
You're welcome! :)
This is better than the modules they show at school
We are really glad to hear the video was helpful. Thanks for watching!
How we get our desired product in pcr if long ends are produced?
Hi Linta,
Thanks for watching and leaving your question!
Would you mind clarifying your question or if the question is in response to a section in our video?
If you would you like to amplify long PCR amplicons there are DNA polymerases specifically engineered to do this with high specificity (such as abm's Long-Range DNA Polymerase)!
Thanks for replying .long fragments are produced but in low quantity and that's why they don't show on gel
@@lintaali6784 Yes, you are correct - in low quantities, they won't show up on the gel. To amplify them, you can use the DNA polymerases specifically engineered for long PCR amplicons, as mentioned in my last comment. Let me know if you have other questions!
Thank you :)
You're welcome :D