Just the note -- the "rpm" (rounds per minute) is not the universal unit for the centrifuge spinning since the rotor diameter can differ and then the applied centrifugal force as well. It is better to use "rcf" (relative centrifugal force) or "g" which counts with these differences and so is more accurate. But anyway, the topic is summarized very nicely in this video, thanks for it! :)
In the organic method, why did you add liquid nitrogen to the sample first and not EDTA? Besides, you never talked about liquid nitrogen as part of the reagents to be used before you started.
Why we add TE buffer at the last part ,and in last step after the purification which reagent we have to add in it and wich wavelength we read it for identify the concentration of dna in the solution ( for protein kinase k)
hello.. great video btw! But where did you read the materials presented? Did you read them from a textbook or from a journal? Can you please specify it? My professor asked me to study DNA extraction methods but I've been having trouble finding a credible source :(
Oh gosh! I'm so sorry. I only know English and the Filipino languages (Tagalog and Kapampangan). :( I hope you were still able to find the video useful. :)
Just the note -- the "rpm" (rounds per minute) is not the universal unit for the centrifuge spinning since the rotor diameter can differ and then the applied centrifugal force as well. It is better to use "rcf" (relative centrifugal force) or "g" which counts with these differences and so is more accurate.
But anyway, the topic is summarized very nicely in this video, thanks for it! :)
Maa Shaa Allah ur video covered all aspects... 👍👍👍👍👍amazaing
Very well explained I hope you have more videos about plant physiology research and expirmentation
Nicely explained. So much better than my professor, can you please do another video explaining the remaining methods
Yes, soon. :)
Excellent - thank you
Excellent explanation 👏👏👏
thank you so much.
In the organic method, why did you add liquid nitrogen to the sample first and not EDTA?
Besides, you never talked about liquid nitrogen as part of the reagents to be used before you started.
Can you please do another video about DNA extraction from fungi
can you please tell, as proteinase K is enzyme(protein) and proteins are organic in nature... how is the method inorganic?
Why we add TE buffer at the last part ,and in last step after the purification which reagent we have to add in it and wich wavelength we read it for identify the concentration of dna in the solution ( for protein kinase k)
Could you pls do a video of rna and plasmid dna extraction as well
Will try to prepare one soon. Subscribe so you get notified when it's ready. :)
hello.. great video btw! But where did you read the materials presented? Did you read them from a textbook or from a journal? Can you please specify it? My professor asked me to study DNA extraction methods but I've been having trouble finding a credible source :(
Thanks more
Can you write. Arabic your explain ?
Oh gosh! I'm so sorry. I only know English and the Filipino languages (Tagalog and Kapampangan). :(
I hope you were still able to find the video useful. :)