2:43 please.. have a question.. what type of solvent you have used for maceration ? I have another question : for ex if we use ethanol-water as solvent, after the extraction we eleminate the ethanol first using ratary-evaporator,but how we eliminate water fraction ? Thanks brother for sharing this video !!
Hi Mira! Thank you so much for your comment. In my own study, I used this paper www.sciencedirect.com/science/article/abs/pii/S030881460900199X. But generally, this video is intended to show how to perform basic plant extraction and discuss what plant extract is. 😊
@DoctorJPTV, how can someone reach you? I’m trying to extract saponins from plant and I was considering using an alcohol like methanol or ethanol as the solvent while I use maceration process for the extraction I’m kinda confused if I should dry my plants first or just use the plant like that And another question, if you can help me with the procedure for maceration extraction I will be grateful Thank you doc!
Hehe! Kawa ka mo ng mga video about make shift lab equipment since hindi naman lahat may boiler at vacuum ek ek. Para sa mga mahilig mag experiment pero wala pambili ng equipment. Hehe
Man I like this laboratory. Thanks for the video. This is the third time I am watching this. So after extraction how do you fortify your product with the extract?
Thnq for such a valuable video sir..... Sir can u pls resolve my quiery...if we want to prepare a plant extract (for any activity) for whom we dnt have any idea about its solubility then which solvent will we use?
Hi! your video helped me so much. But can I ask a few questions to clarify? 1. What is the best solvent to use for decoction method for extracting phenolic content? 2. What is the best solvent to use for decoction method for extracting flavonoid content? Your response would be very much appreciated because I need this for my research methodology. Thanks!!
This video is very insightful. May i ask if you don’t mind, when using the decoction method what is the reasoning behind freezing? And if necessary how will it be possible to obtain a liquid form of the extract instead of the powder? Thank you for any response and for this video.
Hello! Thank you for your comment. To answer your questions, 1st. the reason behind freeze drying is to remove the water content from your extract without deteriorating your bioactive compounds. 2nd. if you want to obtain a liquid form of your extract, it's either you can dissolve the powder in your treatment vehicle (solvent used treatment, depending on your experiment), or you can use the extract automatically after decoction and filtration, but you have to make it more concentrated. If you don't concentrate your extract, it will probably yield lesser bioactivity.
Hello po! Thank you po for the informative video! It really helped us understand the methodology needed to conduct our research. May we ask po how much plant material (in grams) do we need to use to extract 5mg, 16.25mg, 35mg of the pure extract? And also how much solvent is required for maceration? We are currently struggling to compute the amount of grams of plant material to get the according milligrams (We tried using the vague references in the video but still struggled). Also po we need to compute the amount of solvent used for maceration and the ratio is 1:2 (w/v). The volume of our final solution is 50ml and we need to dilute it for 3 concentrations (10%, 32.5%, 70%). After solving this, the grams needed for the dilution is already stated above (5mg, 16.25mg, 35mg). To conclude, our group wants to know how much grams of plant material is needed for the final product using the 1:2 ratio for maceration and how much solvent is required. **We know that this request is a bit hard, if it cannot be answered, do you have any suggestions po on how we can compute it?** Thanks po doc!!
Hi, Sir Doc, I find your video very helpful in our on going project study, I just want to ask if we use boiling of the roots, only through centrifuge is applicable to get the final extract or we can use the rotovap? Thank you Sir! I also subscribed in your youtube channel.
Hello, Sir! Your video helped me a lot in understanding this process which is so hard to learn during online learning. Our study is experimental and we are currently doing our research proposal. I just have a question in mind related to our study, after obtaining the plant extract, how is it usually used to treat the test animal? Just pure or with other substances? Hope you get to answer my question. Thank you!
Very nice sir... I have a question? Someone who don't have fuming hood then how can be evaporated the remaining solvent? As by keeping in open air it takes lot of time to evaporate... ur reply will be appreciated sir...
Hello! Thank you for this informative video. I have 2 questions to which I hope to have an answer to, it would be very much appreciated. 1- In our lab, we don't have a freeze dryer (lyophilisateur), and in a lot of references, i read that they used a drying oven at 40°C for 48h to evaporate to water in the extract. It is okay? Will it not affect my results too much? 2- For your alcohol extract, after using the rotavapor, the extract was liquid meaning there was still a little bit of solvent in it no? if i'm right, how will you then calculate the concentration if you don't know the initial concentration of your extract? Thank you!
1. Yes, you can do that. 2. Yea, there will be some alcohol left. But you need to further evaporate this in a fumehood for a couple of days. Concentration of what? For what? It is automatic that the concentration of the extract you have collected is 100% pure crude extract. If you want to use it as a treatment for testing, then compute the specific concentration you need for the testing using weight of the extract/volume of solvent.
I'm a 4th year pharmacy student and want to be involved with pharmaceutical science research. I've been thinking about taking a Masters in Pharmacy, but I'm second guessing because from what I see the type of research produced is usually assays of plant extracts which I kinda don't like. The type of research I want to do is along the lines of polymers, nanotech, etc. (i haven't quite figured it out yet). Do you have any advice for me?
Hi. This is really informative, can I ask what are the exact volume / measurement of the used materials here? What is the ratio of the plant to solvent? Will there be a difference in the result if the amount of extracted plants are bigger? Thank you.
There’s no need to follow any ratio. As long as you have totally submerged your plant sample in your solvent then you’re good to go. After all, will remove the solvent when you run your mixture in a rotary evaporator.
@@DoctorJPTV thank you! We're planning to make different concentrations for the plant extract to determine which is more effective as antibacterial. How do we measure or make different concentrations of the extract?
@@kaeyaalberich4427 you just have to weigh certain amount of your extract and dissolve it in a certain volume of solvent medium. Example: 10mg of plant extract dissolve in 10mL water will make 1 mg/mL plant extract.
Thank you Doc! laking tulong neto sa gagawin kong thesis... and may I ask if gaano po dapat karami (kg) ng plant ang kelangan to make a 20 mL plant extract?..the plant I'm planning to use is the Chromolaena odorata po Doc.
Actually, mahirap ma-estimate kung gaano kadaming extract ang makakuha mo after extraction. What we do, usually, is prepare as much as we can.. mas maganda na yung sobra kesa kulang. Also, when it comes to the extraction method na gagamitin, nakadepende dapat sa target compounds or family of compounds na gusto mong pag-aral. As I said sa video, different extraction methods will give you different products... and these include volume of tincture/oil/concentrate or yung raw form nung na-extract mo. 😊
Doc, can i store the ethanolic extract in an amber glass bottle before transferring it for rotavap? (our university lack rotavap, and we are using another laboratory for it) How can we store the ethanolic extract safely?
@@DoctorJPTV Thank you. I'm currently doing a stem bark extraction using ethanol as my solvent. Do you know what procedure I could perform to powderized my liquid extract? or to make it a liquid gel? I'd love to hear from you. Thank you
I would like to ask if there are other ways of extracting epicuticular wax as water resistant coating from leaves? We're just going to use it for our capstone project. Thank you!
your rot' evap' setup ... a few questions : 1. what is that thing the sample flask attaches to ? it looks like some sort of splash - guard and does it have a part number ? 2. the condenser glass thing ... does it have a part number too ? you did well with the camera work to capture the p/n's for the rotavapor and heat - bath. with all these p/n's one will be able to shop for them
Estoy trabajando con extractos para mi tesis y no logro eliminar el etanol del agua, me puede explicar a que temperatura esta el refrigerante y cual es el vacio que utiliza.
hello po, yung sa alcohol plant extract po b, after po ung evaporation of excess solvents sa fume hood, I frefreezer din po ba tsaka ifrefreeze dry , para makagawa ng powder? Or hahayaan lng po hanggang magdry sa fume hood yung extract tapos i mortar and pestle para maging powder? Looking forward for your answer po Doc. Thank you!
Hello doc! Pls answer this because I really need it for my team's defense How many extracts will be if I have 3kg of bougainvillea bracts using the maceration method?
I always heard extractions where done with alcohol, butane, propane, and co2 Infusions where done with water, oil, viniger or a low proof alcohol like wine ?
Hey really nice video, so i wanted to extract anthocyanins from Jamun fruit using maceration with ethanol, do I need to dry the fruit or can I just simply put the fruit inside the solvent ?
There is no recommended ratio of solid to solvent when performing maceration because the end step is totally evaporating the solvent. So, as long as you have completely submerged your plant sample in your solvent then it’s good.
Brilliant. thank-you. Question.........If you spend $xxxx for that magnificent rotovap shouldn't you have the expectation that there would be no final "air solvent drying" step. (post-roto)? I mean isn't that the one damn job its supposed to do well? Why not just run it longer or spin it faster or add heat to the bath or pull whatever vacuum it takes before you suck your extract into the bump trap?(that last one was humour) This may not be relevant but everything else was very clear. Thanks
Hmm you’re actually right. But when the extract thicken up it’s very hard to collect from the round flask, most especially if you want to collect most of it (example you have limited resources of plant material). That’s the only reason why we need to save a little solvent. 😊
Good day sir, Is the extract from rotavap the final extract that is used for antimicrobial testing? Thank you so much and Im looking forward to hearing your response😊
@@DoctorJPTV Noted sir. If you don't mind, may I just ask one question po. What is the best alternative to use if our laboratory doesn't have rotavap? Thank you in advance sir☺️
Hi! If you have no plan on retrieving your extraction solvent (alcohol), you can just use a typical water bath. Maintain a certain temperature, use the boiling point of your solvent or somewhere close to that temperature. Then continuously stir your extract during the evaporation process.
Good afternoon, Doc. Can we remove the water using rotary evaporator? We have used 80% ethanol and methanol in the maceration process. However, we are planning to further concentrate the crude extract. What is the alternative way po? Thank you, Doc!
Hello Dr. JP hope all is well, I was wondering if there is a less destructive method of extraction there. All of this heating and drying surely would destroy much of the plant original compounds leaving us is a big chunk of oxidized and a lower grad less therapeutic extract... Would love to hear your answer, thank you.
Personally, I agree with you. However, I don’t thinking you’re making too much alterations or destructions to the bioactive compounds as long as you are in the limits. Hence, it is important to really know the your target bioactive compounds, such as their boiling point, melting point, polarity, solubility, etc.
@@DoctorJPTV thank you for your timely response I am definitely learning something new with your video. But my question remains unanswered if you may. Do we have an alternative to all of this procedure? I am trying to be as puritan as I can in my preperations and know in my gut that there must be a friendlier less destructive alternative. Thank you again 💖
Hi, after you dried the extract in the fume hood, do you get thick & concentrated liquid extract or solid (powder) extract? I am still confuse on how to get plant extract concentration as I got thick liquid extract (I want to calculate in mg/mL just like most research papers). can I just weigh the liquid extracts in mg?
hello! good am I am a 4th-year high school student I just wanna ask what is the best solvent to use for maceration for extracting the components in the plant and also good for the skin?
I have a question, the powder that i have used for the maceration can i still reuse it? After filtering my solution, there was still leftover solute. After some time, it became dry again. And so i was hoping if i could use it again to make another batch of the maceration process. My plant material is very limited, you see. I used methanol as my solvent btw
I’m not sure but I think you need to perform steam distillation to get extract d-limonene.. try checking then form of D-limonene and look what steam distillation is. 😊
Hi sir, would like to ask how to do this extraction using only aqueous solution since my research is about how to get plant extracts in the most economical way possible for farm raisers.
Good day, Doc. Doc, how long did you store your crude extracts in your fume hood for further evaporation? I mean how long does it take to make the crude extract from syrupsy to dried form, if possible? Thank you in advance, Doc.
Water is more polar than ethanol. If you want to extract bioactive compounds specifically those with antimicrobial properties, better check the literature of what Antimicrobial compounds present in your plant and identify in which solvent are they soluble.
Hello. After you put the alcohol extract to the fume hood, did you store it also sa freezer and had it dried through freeze dryer the next day? In this video, you only elaborated the extraction By boiling. Thanks
After putting your extracts in the fumehood, you can use them for assays. If you want to store them, you can put them in a refrigerator. Freeze dryer is used for aqueous extracts.
Another question Doc please, I Will do maceration here but I Need to Ship dry extracts to Hiroshima University. The extracts should be dry. How should I be able to ship my extracts without compromising the compounds? I Will do there HPLC purification, NMR and enzyme assays. Thank you.
@@princessanncuartero7423 yes, you can ship them without damaging the bioactive compounds. Just store it in an amber vial. Cover the vial with a foil. Seal it properly.
what solvent did you use po for the extraction? i am working on my research paper similar with this methodology however i don’t have enough knowledge about it since i am only shs student and i don’t have consultant as well bcs of this pandemic.
Before introducing you treatment (extract) to animal models you should make sure that all of the extraction solvent (ethanol) has completely evaporated. Usually, you can administer your treatment using the common drug delivery methods like oral, intravenous, intraperitoneal, etc. You can also add your treatment to the feed formulation but it will depend on what kind of feeds you have and its nature (dry/wet). Moreover, adding your treatment to the feed formulation might lead to inaccuracies in the volume or concentration of the drug (extract). So, you have to take note of that too. Like for example, if you want to treat your animal model with 200uL of 10 mg/kg extract, you plan how you can administer this accurately to all of your replicates to avoid biases and such.
Hi Doc! We are currently working on a research where are going to extract Peroxidase enzyme from papaya to detect glucose in a urine through GOD-POD method, but we are struggling in finding a laboratory that will help us to extract the Peroxidase from Papaya so that we can have a analytical grade peroxidase that we can use for our research. Do you have laboratories that you can refer so that we can contact them?
Yes, you can. But first, identify your target anticancer compounds. Next, determine in which solvent they are soluble. Then use that solvent to extract your compounds.
I wanna ask how much the ratio of plant sample and solvent do you use. If i use 5g of dried plant how much of ethanol do i need to dissolve the sample?
Hey doc how r u doing that was a great video. Can you please show us fraction distillation of essential oil to extract different components from it or any other technique. For example I want to seperate citrol and Other components
Hello Doc, I would like to ask if it is possible that after decoction, I filter the mixture through a whatman filter paper and freeze dry the liquid extract without the use of a centrifuge and rotary evaporator? (I am using an aqueous extract)
Hello! Sir, may I ask about apple maceration?. We're planning on getting its extract, but we're not sure if we still need to dry them or it's fine to just cut it into small pieces?. Thank you!
please help me to answer this question: What strategies can be employed so that the extraction and isolation of natural products are done under mildest possible conditions?
Hello! I am currently doing my research in plants. I have extracted it with ethanol extract and already airdried it. However, it contains chlorophyll and I am trying to remove it using a separating funnel with non-polar solvents. But there is a lot of confusion, can you explain how can I remove chlorophyll without destroying my active constituents in ethanolic extract also which non polar solvet is best or if there is any other methods.
I have a stupid question but please help, i want to extract nutrients from Seaweed to use it as fertilizer does boiling water denature or destroy any useful amino acids or plant hormones like auxins or cytokinin etc?
Hello sir I have ..I've kept coriander leaves for maceration (for antibacterial property against p.acne) .but only using water I did not use ethanol or methanol with the water ..is it possible to obtain the concentrated extract from it and if so wt should be the temperature maintained in the water bath and also the pressure
Hai, when you freeze dry. Is it ok to fill almost full or leave a small space in your centrifuge tube? Because the technician in the lab that I'm using freeze dryer would tell to fill just half. but I filled the same as in your video. Thanks.
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2:43 please.. have a question.. what type of solvent you have used for maceration ?
I have another question : for ex if we use ethanol-water as solvent, after the extraction we eleminate the ethanol first using ratary-evaporator,but how we eliminate water fraction ?
Thanks brother for sharing this video !!
Wow this is 3yrs video and it's helpful to me this year thank you so much Doc.
Do you have published paper that i can cite you on this method? By the way, thank you for sharing this! Very much appreciated.
Hi Mira! Thank you so much for your comment. In my own study, I used this paper www.sciencedirect.com/science/article/abs/pii/S030881460900199X.
But generally, this video is intended to show how to perform basic plant extraction and discuss what plant extract is. 😊
@@DoctorJPTV Alright, well noted. Thank you for sharing! Love your vid. Simple and easy to understand. 😊😇❤️
Sir I want to work on cannabis but don't know what to do because already some much is done
@DoctorJPTV, how can someone reach you?
I’m trying to extract saponins from plant and I was considering using an alcohol like methanol or ethanol as the solvent while I use maceration process for the extraction
I’m kinda confused if I should dry my plants first or just use the plant like that
And another question, if you can help me with the procedure for maceration extraction I will be grateful
Thank you doc!
Thank you, sir! This has helped me in understanding better the procedures I need to do for my thesis.💚
You're writing a thesis but you need to go on TH-cam to figure out how to run a rotovap?
Hehe! Kawa ka mo ng mga video about make shift lab equipment since hindi naman lahat may boiler at vacuum ek ek. Para sa mga mahilig mag experiment pero wala pambili ng equipment. Hehe
This is a very comprehensive guide
Man I like this laboratory. Thanks for the video. This is the third time I am watching this. So after extraction how do you fortify your product with the extract?
By “fortify” do you mean purify? You need to do chromatic profiling, separation and purification techniques for that.
That was so helpful man! Thank u doc. Stay immensely blessed ✨
big help gyud ni sa ako specially sa school.. thank you kuya John Paul ☺️☺️☺️
Thnq for such a valuable video sir.....
Sir can u pls resolve my quiery...if we want to prepare a plant extract (for any activity) for whom we dnt have any idea about its solubility then which solvent will we use?
Hi! your video helped me so much. But can I ask a few questions to clarify?
1. What is the best solvent to use for decoction method for extracting phenolic content?
2. What is the best solvent to use for decoction method for extracting flavonoid content?
Your response would be very much appreciated because I need this for my research methodology. Thanks!!
1 and 2. You can only use water in decoction method. You’ll be extracting flavonoids and phenolic compounds that are soluble in water only.
This video is very insightful. May i ask if you don’t mind, when using the decoction method what is the reasoning behind freezing? And if necessary how will it be possible to obtain a liquid form of the extract instead of the powder? Thank you for any response and for this video.
Hello! Thank you for your comment. To answer your questions, 1st. the reason behind freeze drying is to remove the water content from your extract without deteriorating your bioactive compounds. 2nd. if you want to obtain a liquid form of your extract, it's either you can dissolve the powder in your treatment vehicle (solvent used treatment, depending on your experiment), or you can use the extract automatically after decoction and filtration, but you have to make it more concentrated. If you don't concentrate your extract, it will probably yield lesser bioactivity.
How we can concentrate the extrait
Hello po! Thank you po for the informative video! It really helped us understand the methodology needed to conduct our research. May we ask po how much plant material (in grams) do we need to use to extract 5mg, 16.25mg, 35mg of the pure extract? And also how much solvent is required for maceration?
We are currently struggling to compute the amount of grams of plant material to get the according milligrams (We tried using the vague references in the video but still struggled). Also po we need to compute the amount of solvent used for maceration and the ratio is 1:2 (w/v). The volume of our final solution is 50ml and we need to dilute it for 3 concentrations (10%, 32.5%, 70%). After solving this, the grams needed for the dilution is already stated above (5mg, 16.25mg, 35mg).
To conclude, our group wants to know how much grams of plant material is needed for the final product using the 1:2 ratio for maceration and how much solvent is required. **We know that this request is a bit hard, if it cannot be answered, do you have any suggestions po on how we can compute it?** Thanks po doc!!
hello sir! What if we don't have rotary evaporator. Can u suggest some alternative for the extraction?
Hi, you can perform distillation method or use a water bath.
@@DoctorJPTV how long po ang water bath sir?
@@aliyah236 until all of the extraction solvent evaporated
@@DoctorJPTV thank you po!
Hi! Can I ask po kung paanong water bath po yung ginawa ninyo? Parang pinakuluan po ba?
Nice content lalo na for students. Very helpful 👏🏻👏🏻👏🏻👏🏻
Thank you for sharing your knowledge po ☺
Hi, Sir Doc, I find your video very helpful in our on going project study, I just want to ask if we use boiling of the roots, only through centrifuge is applicable to get the final extract or we can use the rotovap? Thank you Sir! I also subscribed in your youtube channel.
Hello, Sir! Your video helped me a lot in understanding this process which is so hard to learn during online learning. Our study is experimental and we are currently doing our research proposal. I just have a question in mind related to our study, after obtaining the plant extract, how is it usually used to treat the test animal? Just pure or with other substances? Hope you get to answer my question. Thank you!
It depends on your test and research objectives. You need to have a “vehicle” when introducing your treatment…
@@DoctorJPTV Thank you, SIr!
impressive and practically well-teaching video. thanks
Hi, very informative video, tanong lang is there chance na yung plant extract is very oily? Kasi we did it using a seed and the extract was very oily
This is very informative! Support ito, grabe!
Mommah Juu thank you po!
Very nice sir... I have a question? Someone who don't have fuming hood then how can be evaporated the remaining solvent? As by keeping in open air it takes lot of time to evaporate... ur reply will be appreciated sir...
Hello! Thank you for this informative video. I have 2 questions to which I hope to have an answer to, it would be very much appreciated.
1- In our lab, we don't have a freeze dryer (lyophilisateur), and in a lot of references, i read that they used a drying oven at 40°C for 48h to evaporate to water in the extract. It is okay? Will it not affect my results too much?
2- For your alcohol extract, after using the rotavapor, the extract was liquid meaning there was still a little bit of solvent in it no? if i'm right, how will you then calculate the concentration if you don't know the initial concentration of your extract?
Thank you!
1. Yes, you can do that.
2. Yea, there will be some alcohol left. But you need to further evaporate this in a fumehood for a couple of days.
Concentration of what? For what? It is automatic that the concentration of the extract you have collected is 100% pure crude extract. If you want to use it as a treatment for testing, then compute the specific concentration you need for the testing using weight of the extract/volume of solvent.
hi can u send me your basis? might as well use it in my reswarch thank u
I'm a 4th year pharmacy student and want to be involved with pharmaceutical science research. I've been thinking about taking a Masters in Pharmacy, but I'm second guessing because from what I see the type of research produced is usually assays of plant extracts which I kinda don't like. The type of research I want to do is along the lines of polymers, nanotech, etc. (i haven't quite figured it out yet). Do you have any advice for me?
in oil extraction,how would you compare maceration and cold pressed using a machine,which do you think is more potent?
Hi. This is really informative, can I ask what are the exact volume / measurement of the used materials here? What is the ratio of the plant to solvent? Will there be a difference in the result if the amount of extracted plants are bigger? Thank you.
There’s no need to follow any ratio. As long as you have totally submerged your plant sample in your solvent then you’re good to go. After all, will remove the solvent when you run your mixture in a rotary evaporator.
@@DoctorJPTV thank you! We're planning to make different concentrations for the plant extract to determine which is more effective as antibacterial.
How do we measure or make different concentrations of the extract?
@@kaeyaalberich4427 you just have to weigh certain amount of your extract and dissolve it in a certain volume of solvent medium.
Example:
10mg of plant extract dissolve in 10mL water will make 1 mg/mL plant extract.
You could use vacuum filter to do the filtration faster than using filter paper.
Sir
Please suggest food wastes( plant or vegetables) for the extraction of polyphenols for my dissertation
Thank you Doc! laking tulong neto sa gagawin kong thesis... and may I ask if gaano po dapat karami (kg) ng plant ang kelangan to make a 20 mL plant extract?..the plant I'm planning to use is the Chromolaena odorata po Doc.
Actually, mahirap ma-estimate kung gaano kadaming extract ang makakuha mo after extraction. What we do, usually, is prepare as much as we can.. mas maganda na yung sobra kesa kulang. Also, when it comes to the extraction method na gagamitin, nakadepende dapat sa target compounds or family of compounds na gusto mong pag-aral. As I said sa video, different extraction methods will give you different products... and these include volume of tincture/oil/concentrate or yung raw form nung na-extract mo. 😊
Thank you so much from Brazil
@@Denis-ss1mt glad I helped 😊
one thing i want to ask, can you please share the procedure of solvent extraction for plant bark extract, for its phytochemical analysis.
Dmt nexus
Doc, can i store the ethanolic extract in an amber glass bottle before transferring it for rotavap?
(our university lack rotavap, and we are using another laboratory for it) How can we store the ethanolic extract safely?
pls do a video proceeding to HPLC analysis :)
Him Thanks for this very informative video. Can decoction be used for stem bark?
Yes 😊
@@DoctorJPTV Thank you. I'm currently doing a stem bark extraction using ethanol as my solvent. Do you know what procedure I could perform to powderized my liquid extract? or to make it a liquid gel?
I'd love to hear from you. Thank you
Hi doc! What did you use po for filtration? Which is better po to use for filtration, cartridge of UF membrane or qualitative filter paper?
Awesome video shall I know the best method for extracting Ginseng herb? can we use the same method? please advise and thanking in advance
It really depends on your target bioactive compounds present in Ginseng herb.
Thanks doc. What's the ratio of the extract to solvent?
I would like to ask if there are other ways of extracting epicuticular wax as water resistant coating from leaves? We're just going to use it for our capstone project. Thank you!
your rot' evap' setup ... a few questions :
1. what is that thing the sample flask attaches to ? it looks like some sort of splash - guard
and does it have a part number ?
2. the condenser glass thing ... does it have a part number too ?
you did well with the camera work to capture the p/n's for the rotavapor and heat - bath.
with all these p/n's one will be able to shop for them
Estoy trabajando con extractos para mi tesis y no logro eliminar el etanol del agua, me puede explicar a que temperatura esta el refrigerante y cual es el vacio que utiliza.
hello po, yung sa alcohol plant extract po b, after po ung evaporation of excess solvents sa fume hood, I frefreezer din po ba tsaka ifrefreeze dry , para makagawa ng powder? Or hahayaan lng po hanggang magdry sa fume hood yung extract tapos i mortar and pestle para maging powder?
Looking forward for your answer po Doc. Thank you!
Hello doc!
Pls answer this because I really need it for my team's defense
How many extracts will be if I have 3kg of bougainvillea bracts using the maceration method?
I always heard extractions where done with alcohol, butane, propane, and co2
Infusions where done with water, oil, viniger or a low proof alcohol like wine
?
Hey really nice video, so i wanted to extract anthocyanins from Jamun fruit using maceration with ethanol, do I need to dry the fruit or can I just simply put the fruit inside the solvent ?
Preferably dry the fruit to saturate the concentration of anthocyanin. Since anthocyanins are light sensitive make sure to dry it on a dark areas.
hello doc, your video is a big help to me, May I ask, what is the recommended ratio of solid to solvent when performing maceration. thank you so much
There is no recommended ratio of solid to solvent when performing maceration because the end step is totally evaporating the solvent. So, as long as you have completely submerged your plant sample in your solvent then it’s good.
Brilliant. thank-you. Question.........If you spend $xxxx for that magnificent rotovap shouldn't you have the expectation that there would be no final "air solvent drying" step. (post-roto)? I mean isn't that the one damn job its supposed to do well? Why not just run it longer or spin it faster or add heat to the bath or pull whatever vacuum it takes before you suck your extract into the bump trap?(that last one was humour) This may not be relevant but everything else was very clear. Thanks
Hmm you’re actually right. But when the extract thicken up it’s very hard to collect from the round flask, most especially if you want to collect most of it (example you have limited resources of plant material). That’s the only reason why we need to save a little solvent. 😊
Good day sir, Is the extract from rotavap the final extract that is used for antimicrobial testing? Thank you so much and Im looking forward to hearing your response😊
Yes. But make sure that the solvent or alcohol used has completely evaporated.
@@DoctorJPTV Noted sir. If you don't mind, may I just ask one question po. What is the best alternative to use if our laboratory doesn't have rotavap? Thank you in advance sir☺️
Hi! If you have no plan on retrieving your extraction solvent (alcohol), you can just use a typical water bath. Maintain a certain temperature, use the boiling point of your solvent or somewhere close to that temperature. Then continuously stir your extract during the evaporation process.
@@DoctorJPTV Thank you sir. God bless you always❣️
Good afternoon, Doc. Can we remove the water using rotary evaporator? We have used 80% ethanol and methanol in the maceration process. However, we are planning to further concentrate the crude extract. What is the alternative way po? Thank you, Doc!
great work sir , can you help me with a way to extract tannin from a plant ?
so the vapour temperature is basically the solvent's boiling/evaporation point?
Hi doc, can you make content about extracting polyglutamic acid from soybean and turning into a powder?
Hello Dr. JP hope all is well, I was wondering if there is a less destructive method of extraction there. All of this heating and drying surely would destroy much of the plant original compounds leaving us is a big chunk of oxidized and a lower grad less therapeutic extract... Would love to hear your answer, thank you.
Personally, I agree with you. However, I don’t thinking you’re making too much alterations or destructions to the bioactive compounds as long as you are in the limits. Hence, it is important to really know the your target bioactive compounds, such as their boiling point, melting point, polarity, solubility, etc.
@@DoctorJPTV thank you for your timely response I am definitely learning something new with your video.
But my question remains unanswered if you may. Do we have an alternative to all of this procedure? I am trying to be as puritan as I can in my preperations and know in my gut that there must be a friendlier less destructive alternative. Thank you again 💖
You can try decoction method. However, using this method you can only extract water soluble bioactive compounds.
@@DoctorJPTV ok got you Dr. 👌 a LOT of lerning still lays ahead for me 🙏
What type filter paper was used in the filtration? Is it a Whatman filter paper? If so, what grade of Whatman filter paper was used?
Whatman is okay.
Thanks doc. Learned a lot
Hi, after you dried the extract in the fume hood, do you get thick & concentrated liquid extract or solid (powder) extract? I am still confuse on how to get plant extract concentration as I got thick liquid extract (I want to calculate in mg/mL just like most research papers). can I just weigh the liquid extracts in mg?
Hello, sir! I would like to ask lang po kung ano ang solvent-solute ratio po ang ginamit niyo? Thank you po!
Hi sir. Are there any alternatives for rotary evaporator? Can you use simple heating to remove the solvent?
Hello doc May I know which solvent is effective in plant extracts like leaves, roots, seeds and barks?
@@DIGIWAL_ART it depends on what bioactive compounds you want to extract. Bioactive compounds have different polarity.
Thank you brotherJohn Paul
Good day! The plant powder that you used in the beginning of the video, was it store-bought or did you prepare the powder extract? Thank you!
We prepared the plant powder. We pulverize the leaves using a mechanical miller.
hello! good am I am a 4th-year high school student I just wanna ask what is the best solvent to use for maceration for extracting the components in the plant and also good for the skin?
Thank you for sharing but I need more clarifications how can you help me?
I have a question, the powder that i have used for the maceration can i still reuse it? After filtering my solution, there was still leftover solute. After some time, it became dry again. And so i was hoping if i could use it again to make another batch of the maceration process. My plant material is very limited, you see. I used methanol as my solvent btw
You can but the amount of bioactive compounds might be lesser than the first batch.
Good day doc! I just want to ask if you have any home based alternative for extracting D-limonene from citrus peels?
I’m not sure but I think you need to perform steam distillation to get extract d-limonene.. try checking then form of D-limonene and look what steam distillation is. 😊
@@DoctorJPTV thank you doc! 🙏
Good day, I would like to ask if what plant extraction is applicable to acquire its epicuticular wax from it?
Hi sir, would like to ask how to do this extraction using only aqueous solution since my research is about how to get plant extracts in the most economical way possible for farm raisers.
Good day, Doc. Doc, how long did you store your crude extracts in your fume hood for further evaporation? I mean how long does it take to make the crude extract from syrupsy to dried form, if possible? Thank you in advance, Doc.
Around 1-3 days.. it depends.. you’ll know it’s ready if you can’t smell the solvent from your extract and you can observe that it has dried up.
Thanks, Doc!
Hi! May I ask what is the proper measurement of your solvent and your grind products?
Hi! For this experiment, I used 95% ethanol and my plant material was powdered Corchorus olitorius leaves.
Good day Doc. Please which is a better polar solvent between aqueous and ethanol in extracting plants bioactive compounds against microbes?
Water is more polar than ethanol. If you want to extract bioactive compounds specifically those with antimicrobial properties, better check the literature of what Antimicrobial compounds present in your plant and identify in which solvent are they soluble.
Great video!
Hi Sir, how many plant powder and solvent during maceration in this video? Thankyou
Hi sir, what do you think about making herbal extract with glycerine
You can use glycerine to extract your compounds from your plant sample if your target compounds are highly soluble in glycerine.
How to chose best solvent for Phthalates extraction? GC-MS analysis
Hello. After you put the alcohol extract to the fume hood, did you store it also sa freezer and had it dried through freeze dryer the next day?
In this video, you only elaborated the extraction By boiling. Thanks
After putting your extracts in the fumehood, you can use them for assays. If you want to store them, you can put them in a refrigerator.
Freeze dryer is used for aqueous extracts.
Another question Doc please, I Will do maceration here but I Need to Ship dry extracts to Hiroshima University. The extracts should be dry. How should I be able to ship my extracts without compromising the compounds? I Will do there HPLC purification, NMR and enzyme assays. Thank you.
@@princessanncuartero7423 yes, you can ship them without damaging the bioactive compounds. Just store it in an amber vial. Cover the vial with a foil. Seal it properly.
Thanks Doc!
How'd you identify what ratio of solute:solvent to use?
what solvent did you use po for the extraction? i am working on my research paper similar with this methodology however i don’t have enough knowledge about it since i am only shs student and i don’t have consultant as well bcs of this pandemic.
I used 99% analytical grade ethanol. If you need some help on your research paper, feel free to email me at doctorjohnpaulmedia@gmail.com.
thanks. one query is if you have prepared ethanol extract then how to incorporate it in feed during feed formulation.?
Before introducing you treatment (extract) to animal models you should make sure that all of the extraction solvent (ethanol) has completely evaporated. Usually, you can administer your treatment using the common drug delivery methods like oral, intravenous, intraperitoneal, etc. You can also add your treatment to the feed formulation but it will depend on what kind of feeds you have and its nature (dry/wet). Moreover, adding your treatment to the feed formulation might lead to inaccuracies in the volume or concentration of the drug (extract). So, you have to take note of that too. Like for example, if you want to treat your animal model with 200uL of 10 mg/kg extract, you plan how you can administer this accurately to all of your replicates to avoid biases and such.
Hi sir, thanks for the vid, learned a lot. May question po sana ako, pano nyo po ginawa yung sa process ng maceration?
You just have to completely soak your plant sample (better if cut in very small pieces or pulverized) for a couple of days, like 3-7 days.
Hi Doc!
We are currently working on a research where are going to extract Peroxidase enzyme from papaya to detect glucose in a urine through GOD-POD method, but we are struggling in finding a laboratory that will help us to extract the Peroxidase from Papaya so that we can have a analytical grade peroxidase that we can use for our research.
Do you have laboratories that you can refer so that we can contact them?
Are you using Papaya leaves or Papaya fruit?
Hey..can we extract anticancerous constituent from plant by this method of extraction?
Yes, you can. But first, identify your target anticancer compounds. Next, determine in which solvent they are soluble. Then use that solvent to extract your compounds.
I wanna ask how much the ratio of plant sample and solvent do you use. If i use 5g of dried plant how much of ethanol do i need to dissolve the sample?
what solvent did you use in maceration of the plant? :)
I used different extraction solvents. I used ethanol, methanol, DCM, hexane, toluene, etc.
hello po doc JPTV, how am I able to know if suitable po yung particular extraction process sa target ko na makuhang compound po sa plant material?
You can use the literature. You can take note of the most commonly used solvents to extract your target compounds. Also, you can follow their methods.
your video is super helpful. thank you so much
Thank you ❤️
Hey doc how r u doing that was a great video. Can you please show us fraction distillation of essential oil to extract different components from it or any other technique. For example I want to seperate citrol and Other components
Hi! Due to the COVID-19 pandemic, our lab is currently closed. I’ll make a video as soon as our lab opens. 😊
Hello, Sir! Can I ask why does the plant extract needs to be freezed?
Freezing your aqueous extract (decoction method) is important to ensure that sublimation rather than melting will occur when freeze drying.
How long does it take po bago ma evaporate lahat ng solvents? Like how many hours po
Hello Doc, I would like to ask if it is possible that after decoction, I filter the mixture through a whatman filter paper and freeze dry the liquid extract without the use of a centrifuge and rotary evaporator? (I am using an aqueous extract)
Yes, you can. 😊
Hello! Sir, may I ask about apple maceration?. We're planning on getting its extract, but we're not sure if we still need to dry them or it's fine to just cut it into small pieces?. Thank you!
Just cut them in smaller pieces. If you have a dehydrator you can also do it then pulverized the fruit prior to maceration.
@@DoctorJPTV noted, thank you so much!!
Can i ask for list of all lab materials you used in the process?
Hi. you look amazing. Can you help me how to do dna extraction of deadwood ? I mean the best technique
Hi! My knowledge about DNA extraction is quite limited. Maybe you can check recent literatures about that.
@@DoctorJPTV
please help me to answer this question: What strategies can be employed so that the extraction and isolation of natural products are done under mildest possible conditions?
Use lesser heat as possible to prevent the compounds from degradation. For isolation, prepare your samples as clean and as accurate as possible.
Hello! I am currently doing my research in plants. I have extracted it with ethanol extract and already airdried it. However, it contains chlorophyll and I am trying to remove it using a separating funnel with non-polar solvents. But there is a lot of confusion, can you explain how can I remove chlorophyll without destroying my active constituents in ethanolic extract also which non polar solvet is best or if there is any other methods.
use n-hexane
I have a stupid question but please help, i want to extract nutrients from Seaweed to use it as fertilizer does boiling water denature or destroy any useful amino acids or plant hormones like auxins or cytokinin etc?
Hello sir I have ..I've kept coriander leaves for maceration (for antibacterial property against p.acne) .but only using water I did not use ethanol or methanol with the water ..is it possible to obtain the concentrated extract from it and if so wt should be the temperature maintained in the water bath and also the pressure
Question, how much volume is left or acquired after using rotary evaporator?
It depends on the amount of your plant material.
Hi sir, may I ask how many grams are in the vial bottle after rotary evaporation. Thank you
musta na mga lods
Hai, when you freeze dry. Is it ok to fill almost full or leave a small space in your centrifuge tube? Because the technician in the lab that I'm using freeze dryer would tell to fill just half. but I filled the same as in your video. Thanks.
We also fill half of our containers.
Hi, did you get the plant powder by using mortar and pestle?
We freeze-dried our plant sample and pulverized it using a milling machine.
@@DoctorJPTV
How do you dry a plant? What is the best way to dry roots without a lost effective compound?
Im interested in plant extraction and recrystalization can you do a video for that