Plant Tissue Culture Media Preparation

PhytoTechnology Laboratories presents Media Preparation, an informative video highlighting the steps to prepare plant tissue culture and micropropagation media from powdered formulations.
For plant tissue culture media, supplies, equipment, biochemicals, antibiotics, and plant growth regulators, please visit our website at www.phytotechlab.com. You can also become a fan of PhytoTech Labs on facebook to keep informed of new product introductions, recent laboratory studies, and other news.
For technical support, please contact [e-mail]

[@monikandafuda4554]

simplest way of learning I learnt a lot from It very educating. Thank you for being there for me.

[@kowsalyamarimuthu5083]

Nice video

[@muddypawswanderlust]

How do you deal with too much condensation and plant vitrification?

[@PhytoTechLabs]

We recommend measuring & adjusting the media pH after adding supplements, including gelling agents. Agar can have a wide pH range. Sodium hydroxide and other bases are used to increase the gel strength of some agars during their manufacture; this can result in a pH range from slightly acidic to basic (pH 9+). It is for this reason that we recommend adding it before checking the pH of your medium.
[Response continued on next posting]

[@johncarinamartin6716]

PhytoTechnology Laboratories aaaa

[@PhytoTechLabs]

@Gemthermakleingk Yes, this is done intentionally with polypropylene containers like these. The lids seal tightly which results in deformed containers when the pressure drops at the end of the autoclave cycle. This is circumvented by leaving the lids slightly ajar and then closing them when the autoclave is opened at the end of the cycle. This is not a problem with baby food jars using our PhytaCaps or Magenta B-Caps.

[@goldslinger]

Thank You so much for Your continued effort with these videos!
Please keep them coming.
Gary
Garden City, KS

[@g-phoria]

#plant #tissue #culture #medium
Great information and #demonstration

[@PhytoTechLabs]

If you’re using an agar with a high pH and adding it after adjusting the pH of your media then it could cause your media’s pH to increase without your knowledge. In many cases this may have negligible impact on your cultures… unless you’re culturing plant tissues where the pH needs to be more tightly controlled.
Feel free to contact our technical support if you should have any additional questions.

[@alinnicolau1077]

where can i buy all these substances i need?
I want to do an experiment for blueberries

[@tulsiramdagur9580]

Iam also

[@MilonaDupreeVlogs]

So if I use pressure cooker how many minutes needed for that?

[@aravindchary9132]

it is very help full to me for my project

[@maleeshamadubhashini2128]

Thank you so much ❤️

[@TheBgur123]

Check out the book "Plants From Test Tubes, An Introduction to Micropropagation" by Lydiane Kyte & John Kleyn. This will help you very much. It also includes how to build a lab in your house which may be useful for you.

[@TlalocChak]

and thank you for the tips on plant propagation via tissue culture/in vitro.

[@criscarmen524]

I want to enroll for this. can you help where

[@gp235801]

Your pH electrode will be screwed if you adjust the pH after adding agar. I always add the agar separately to the medium after taking pH. Been doing this since 12 yrs now and never had any problems. :)

[@adilnisar8629]

this is very helpful for us ..... thanks

[@milkasusan]

Sir why sucrose is not added to stock solution?

[@aelexpatrick303]

Nice video

[@PhytoTechLabs]

On the contrary, as long as the agar is not melted it will have little effect on your electrode. Actually, measuring the pH of media containing proteinaceous components (e.g., peptones, typtone, casein hydrolysate) is more likely to coat your electrode and reduce its sensitivity.
[Response continued on next postings]

[@Macronvargas]

PhytoTechnology Laboratories could you please help me with a couple of things?

[@1982myo]

It is great!
Thanks

[@yadesabayisa9717]

i like this so much,

[@iqbalkh9009]

thank u good work

[@সৈয়দমোশাররফহোসেন]

Please add the video label of Used Material Name, Process time, Mixing time's pH, Temperature and other issue...

[@dileshbansoodeb4962]

I want to buy the agar micropropagation A111 and what more help

[@grahamalex2765]

good vid thanks

[@dilshaddavood]

How many gram for one litter ?

[@baltiwalibaji5907]

Sir I'm facing problem in making ms media. Sometimes plant grows well while sometimes no growth is observed

[@gouharali3697]

Very helpful

[@PhytoTechLabs]

@340070007
Most plant growth regulators are routinely autoclaved in the medium, especially the stable ones such as BA & NAA. Some are heat labile and should be filter sterilized and added after the medium is autoclaved and cooled a while. Our "Plant Growth Regulators, Part II" document on the Technical Info page on our web site offers guidelines for whether the plant growth regulator can be autoclaved in the medium or filter sterilized.
Gary Seckinger
Technical Services
PhytoTechnology Labs

[@tulsiramdagur9580]

Hello friend,
Blue berry plants Riqar ms midia prportion

[@phillipkoether6087]

You thought we wouldn't notice 0:23

[@shanglemsipong1138]

???????

[@iguniaigunia8488]

Has someone tried this? Is that wiek?

[@iguniaigunia8488]

Work*

[@udjiko]

thank you :)

[@baltiwalibaji5907]

Precipitation is another issue

[@baochengsun7257]

Is vitamins Heat stable?

[@PhytoTechLabs]

Most vitamins used in plant tissue culture are heat stable.

[@baochengsun7257]

thansks!

[@pankajkamboj6434]

Sir I am facing problem in making Plant gel Media . Sterile media Temp.121 c for 17 Min 1.2 Pressure . but Media not proper Solidify PH 5.95 Vertical Shape Autoclave

[@ЕленаЧолич-к3у]

Скажите а можно у вас купить фласки? И сколько стоят?

[@omstshankarmeghasahu4694]

Wow

[@abhishekdhumal2525]

nice

[@laxmikantaghorai9786]

Orchid culture medium Name

[@lordmazinger]

Well done

[@TlalocChak]

that girl is so cute and smart :) very appealing. ^^

[@amrosama6529]

I HATE PHARMACOGNOCY DEPARTEMENT