I can't express enough how much into the science I am with this hobby! Thank you for making this video. I'm curious to know if the protocols you used are generally available online for "common" plants like pothos. I understand why they are a well guarded secret, but I've seen a lot of small nurseries here in California (as well as instagrammers) performing tissue culture in their back rooms. So it makes me wonder how readily available (or shared) certain protocols are seeing so many hoods outside of a hospital or lab setting.
I'm so with you on this, Chad! TC is what really tickles my brain in the plant world. I find it fascinating! I think there are tons of protocols that are good starting points available online. In my four years of experience playing with TC, I've found each species, and potentially sub-species, needs its own curated recipe for that EXACT plant. This can be tough because I usually make 1 Liter of Multiplication media at a time, and that fills 20-30 vessels. So I usually just put Monstera, Philo, Epi, all in the generic Multiplication Media I've made for that day. But I would say I change the mix almost every time I make it!
@@prettyingreen Thanks for the tips! I'm not jumping into tissue culture just yet, but if I do I may want to experiment with a Pink Pineapple, or other patented plants like the Philo Gabby.
I can understand your concerns.. I am trained for TC, I work in the biotechnology field, and yes protocols are well guarded because it's intellectual property. But if you understand how it works an learn the basics, it's not that complicated :) Personnaly I prefer not to do this at home because the quality of the materials and equipements can cause issues... and of course knowing how to manipulate under a BSC requires some training. I think it's best to leave this kind of stuff to people that really know what they are doing and have real lab conditions 😅
I'm a pharmacy technician by trade and I specifically work in a sterile admixtures lab where I utilize aseptic technique to prepare drugs for intravenous use. I find the process of tissue culturing really fascinating and I kind of want to try it myself since it seems to fall in my lane. I'm a little curious about your setup, though, if you don't mind some questions. In a typical laminar flow hood, the air flow is in sheets (hence "laminar flow") from a singular direction. I could be totally wrong, but I don't think that can be achieved with the type of filter you're using. We also utilize PPE including (but definitely not limited to) sterile gloves to avoid introducing potential pathogens. I know I'm working with more sensitive mixtures, considering their administration, but do these practices not impact your process? I'm legitimately curious because I'd like to see if this is something I could set up at home and while a fully sterile environment would be impractical, I could probably rig something up if it doesn't need to be 100%!
It's nice to see how you explain everything about TC. I myself working on Plant tissue culture where I have to induce callus. I have used reported PGR for callusing but it seems like any hormone and its concentration has no effect on my leaf explant which is concerning. Also I'm using both young and mature leaves. What could be a reason for my leaf explant to not give any response on any callus inducing PGR? If you could answer my query?
Wow, so I have watched about 8 videos pertaining to this subject and I wish I would have just started with this one. This is a very well put together video. I really like your brevity and concise instruction. I think TC is going to be my new hobby. Thanks for sharing the video.
could someone answer a question for me? I understand the concept of tissue culture BUT how is it any different from propagation aside from the sterile environment and the more nutrient rich growth medium. I just wonder, if the cutting and everything is all the same, is the only difference a more controlled environment? This may be a stupid question. Lol. I'm just trying to understand exactly how it works and why it's preferred to regular propagation? Especially with plants that aren't too difficult to propagate to begin with. EDIT....maybe I should have finished the video first. Lol. I think I understand a bit better. This was a much more in depth explaination than other videos I've seen. Very interesting
Undifferentiated cells AKA stem cells, exists in the plants structure, like the stem. "Cuttings" typically have one node from which one plant can grow out from. But with the activation of stem cells, multiple plants can grow from one stem. The protocols (aka the growth medium mix) can activate stem cells to grow whatever we want. The initial phase are leaves (to create new clones) second phase is the activation of the roots.
@@ChadGardenSinLA thank you! I think I wasn't realizing that the cutting wasn't only growing normally from the node in a perfect environment but also multiplying quickly due to the stem cells. This video and your comment explained all of this much better than other videos I have watched about it.
This is so interesting. It’s not easy, but hopefully more TC will enable me to get a Thai less expensive. Thanks so much for your work on TC & for sharing!
@@prettyingreenOK....ive come back to this comment because I was looking for someone who knows about tissue culture and can maybe help me with a plant problem I'm having. Basically, I had an apparently healthy plant that rotted quickly and I'm wondering if it could be related to TC. I'llstry to keep it short but give the important details. About a month ago I ordered an N'joy pothos. I got it the next week and it looked beautiful. I let it just sit for a couple days but then repotted it because it was just in a soggy coir mix. The roots looked good....white, maybe a bit thin, but healthy for sure. Each individual plant (I think there were 6) were all crowded in the very center of the pot and there were a LOT of little sprouts, too small to identify as pothos or not. I separated, repotted, and after about a week I noticed they weren't perking up the way I expected. I tugged on the plants and almost ALL of them just came right out and were black and rotted under the soil. Could this possibly be because it wasn't hardened off after TC? And could those sprouts have been because it was still a bit exposed to the hormones and making babies? I don't think it could be the soil because I potted the pothos with another plant and it is just as happy as ever. It also couldn't be over watering. If it were pests or already rotted, I would have seen the evidence when removing the old soil....thanks so much if you see this LONG comment! I'm just trying to figure it out and I'm hoping I don't need to worry about my adansonii (the plant it was potted with) . Could it be because it wasn't hardened off properly? Also, if it's important I got it on amazon from California tropicals.
I am really looking forward to the future tissue culture videos. I harbor delusions of my own little lab some day. Another video topic that you might want to cover is light & variagation. I see conflicting advice and in my experience when increasing light should improve variagation, it does not. (I'm looking at you, cebu blue ghost). I've never had a plant revert. How does variagation happen? How is it replicated in tc and when can it not be? So many questions!
Hello . I love your videos. Im really interested to learn more about tissue culture. All the tools and compounds needed. I really want to try it at home.
Can be a long time if one is proving their clones as stable, healthy and hardy for long life in normal conditions before distributing to the public. Short answer is maybe 3 months from plant cutting to sell question mark plants in dirt with minimal acclimatization.
Correct! I was using a Golden Pothos in the video bc it likely will have contamination due to me talking through the entire video without the laminar flow hood on, and handling it incorrectly
Hello I got a Philodendron Pink Princess that has no grow points left. Unfortunately it would produce only pink leaves which is why that part died off and now I am left with only one initial leaf (from the cutting) and no growth point. I check the roots after 7 months of no visible new shoots. Is that plant doomed? Or is the only thing I could do to try it with tissue culture?
@@prettyingreen hey thanks for replying! So I just need to hormones into the soil? I have rooting hormone. I need to check if I can get my hands on some BAP Hormones.
@@prettyingreen ou wow ok thats great! thanks so much for this information! Ill try that to safe my pink princess… because the rootsystem looks very strong. :)
Is plant also cleaned from viruses and diseases which are inside plant and does't allow plant to give full potential? Does tissue cultured plant have bigger yield?
I’ve found TC doesn’t necessarily breed weak plants. It can at times due to somalclonal variation from repeated sub culturing, but that can also result in positive benefits too.
so, basically the ,,you can make a new plant from every part of the plant, even from a piece of leaf" part is bs and you literaly propagate the plant by node, just on a fancy way
It's not laminar flow. Also, you shouldn't use a magnetic stirrer for sterilizing explants, the magnet can damage cells and cause somatic mutations.@@prettyingreen
I'm A/l agri student so this video is a very important for me.And i like this video .thank u so much❤❤
Glad to hear that 🙌
I think this may be my favorite video you've done so far❤🎉
Aww thank you!!! I tried something a little different this week -- glad to hear you enjoyed :)
I can't express enough how much into the science I am with this hobby! Thank you for making this video. I'm curious to know if the protocols you used are generally available online for "common" plants like pothos. I understand why they are a well guarded secret, but I've seen a lot of small nurseries here in California (as well as instagrammers) performing tissue culture in their back rooms. So it makes me wonder how readily available (or shared) certain protocols are seeing so many hoods outside of a hospital or lab setting.
I'm so with you on this, Chad! TC is what really tickles my brain in the plant world. I find it fascinating! I think there are tons of protocols that are good starting points available online. In my four years of experience playing with TC, I've found each species, and potentially sub-species, needs its own curated recipe for that EXACT plant. This can be tough because I usually make 1 Liter of Multiplication media at a time, and that fills 20-30 vessels. So I usually just put Monstera, Philo, Epi, all in the generic Multiplication Media I've made for that day. But I would say I change the mix almost every time I make it!
@@prettyingreen Thanks for the tips! I'm not jumping into tissue culture just yet, but if I do I may want to experiment with a Pink Pineapple, or other patented plants like the Philo Gabby.
I can understand your concerns..
I am trained for TC, I work in the biotechnology field, and yes protocols are well guarded because it's intellectual property.
But if you understand how it works an learn the basics, it's not that complicated :)
Personnaly I prefer not to do this at home because the quality of the materials and equipements can cause issues... and of course knowing how to manipulate under a BSC requires some training.
I think it's best to leave this kind of stuff to people that really know what they are doing and have real lab conditions 😅
@@aroidaddiction I have Biotechnology diploma 🙂 bio tech is a very wide field and used for much things
TC is biotechnology !
@@alienperfection973 i'll do what i want thanks, easy to find studies with protocols
Check your flowhood air vortex. Sometimes, the way you mive your air around can create pockets of suction bringing bad air in the flowhokd.
I'm a pharmacy technician by trade and I specifically work in a sterile admixtures lab where I utilize aseptic technique to prepare drugs for intravenous use. I find the process of tissue culturing really fascinating and I kind of want to try it myself since it seems to fall in my lane. I'm a little curious about your setup, though, if you don't mind some questions.
In a typical laminar flow hood, the air flow is in sheets (hence "laminar flow") from a singular direction. I could be totally wrong, but I don't think that can be achieved with the type of filter you're using. We also utilize PPE including (but definitely not limited to) sterile gloves to avoid introducing potential pathogens. I know I'm working with more sensitive mixtures, considering their administration, but do these practices not impact your process?
I'm legitimately curious because I'd like to see if this is something I could set up at home and while a fully sterile environment would be impractical, I could probably rig something up if it doesn't need to be 100%!
You can make an environment sterile enough by using a still air box, I'm sure his flow hood is fine :D
This is fascinating! Thank you for the information!!
Thank you for sharing the knowledge. Blessings to you 🙏🏻
It's nice to see how you explain everything about TC. I myself working on Plant tissue culture where I have to induce callus. I have used reported PGR for callusing but it seems like any hormone and its concentration has no effect on my leaf explant which is concerning. Also I'm using both young and mature leaves.
What could be a reason for my leaf explant to not give any response on any callus inducing PGR?
If you could answer my query?
Thank you so much its very informative and easy to understand
I’ve always wondered…
Thanks for sharing!❤️
Absolutely! 😄
Wow, so I have watched about 8 videos pertaining to this subject and I wish I would have just started with this one. This is a very well put together video. I really like your brevity and concise instruction. I think TC is going to be my new hobby. Thanks for sharing the video.
Glad you enjoyed it! TC is really fun and challenging :)
Amazing, thank you so much for sharing this information, I enjoy learning about TC and the future of using it.
Absolutely! I enjoyed making this video 😄 More like it to come!
Superb presentation !
Thank you!!!
This is great information, thanks for dumbing this down. So interesting
So glad you enjoyed!! 😄
What kind of jelly did you use inside the bottle
Agar agar? I think it's a type of gelatin like seaweed
Same question here
It’s agar agar @@queeniefelixjoiedomantay1913
@@beanerb822yes but with plant food and other hormones. This process would be hard to replicate in a home due to the lack of a sterile environment.
Aeroplane
excellent video, thanks for sharing.
What antioxidant do you use for monstera? I have had serious problems with oxidation.
Thanks
could someone answer a question for me? I understand the concept of tissue culture BUT how is it any different from propagation aside from the sterile environment and the more nutrient rich growth medium. I just wonder, if the cutting and everything is all the same, is the only difference a more controlled environment? This may be a stupid question. Lol. I'm just trying to understand exactly how it works and why it's preferred to regular propagation? Especially with plants that aren't too difficult to propagate to begin with. EDIT....maybe I should have finished the video first. Lol. I think I understand a bit better. This was a much more in depth explaination than other videos I've seen. Very interesting
Undifferentiated cells AKA stem cells, exists in the plants structure, like the stem. "Cuttings" typically have one node from which one plant can grow out from. But with the activation of stem cells, multiple plants can grow from one stem. The protocols (aka the growth medium mix) can activate stem cells to grow whatever we want. The initial phase are leaves (to create new clones) second phase is the activation of the roots.
@@ChadGardenSinLA thank you! I think I wasn't realizing that the cutting wasn't only growing normally from the node in a perfect environment but also multiplying quickly due to the stem cells. This video and your comment explained all of this much better than other videos I have watched about it.
This is so interesting. It’s not easy, but hopefully more TC will enable me to get a Thai less expensive. Thanks so much for your work on TC & for sharing!
This is exactly the beauty and speed TC provides over typical stem propagation!
@@prettyingreenOK....ive come back to this comment because I was looking for someone who knows about tissue culture and can maybe help me with a plant problem I'm having. Basically, I had an apparently healthy plant that rotted quickly and I'm wondering if it could be related to TC. I'llstry to keep it short but give the important details. About a month ago I ordered an N'joy pothos. I got it the next week and it looked beautiful. I let it just sit for a couple days but then repotted it because it was just in a soggy coir mix. The roots looked good....white, maybe a bit thin, but healthy for sure. Each individual plant (I think there were 6) were all crowded in the very center of the pot and there were a LOT of little sprouts, too small to identify as pothos or not. I separated, repotted, and after about a week I noticed they weren't perking up the way I expected. I tugged on the plants and almost ALL of them just came right out and were black and rotted under the soil. Could this possibly be because it wasn't hardened off after TC? And could those sprouts have been because it was still a bit exposed to the hormones and making babies? I don't think it could be the soil because I potted the pothos with another plant and it is just as happy as ever. It also couldn't be over watering. If it were pests or already rotted, I would have seen the evidence when removing the old soil....thanks so much if you see this LONG comment! I'm just trying to figure it out and I'm hoping I don't need to worry about my adansonii (the plant it was potted with) . Could it be because it wasn't hardened off properly? Also, if it's important I got it on amazon from California tropicals.
Thank you for sharing! What an amazing concept. Good job on this video and detailed explanation!
Ahhhh thank you Rose!!
I am really looking forward to the future tissue culture videos. I harbor delusions of my own little lab some day.
Another video topic that you might want to cover is light & variagation. I see conflicting advice and in my experience when increasing light should improve variagation, it does not. (I'm looking at you, cebu blue ghost). I've never had a plant revert. How does variagation happen? How is it replicated in tc and when can it not be? So many questions!
I think it's very much possible to create an "affordable" tissue lab for
@@prettyingreen I have a lot of questions. 😆 Thank you for the informative videos!
Very informative .🎉
🤗
Hello . I love your videos. Im really interested to learn more about tissue culture. All the tools and compounds needed. I really want to try it at home.
This is truly incredible indeed.
It is! Big fan of TC
Do you guys doing raspberry tc?
Would the formula same as any plants that i can refer to?
Should mention if tissue culture plants or not by all sellers.
Dr. Hussain
A beautiful and wonderful channel, but I have a question. How long does plant tissue culture take until the seedlings are ready for sale? Thank you
Can be a long time if one is proving their clones as stable, healthy and hardy for long life in normal conditions before distributing to the public. Short answer is maybe 3 months from plant cutting to sell question mark plants in dirt with minimal acclimatization.
What a great video 👍
So great
When in the process do you usually transfer your tissue cultures into semi-hydro environment vrs soil?
Is it possible to make plant media at home, what ingredients are in growing medium. Possible to assist me sir
I think economically it makes sense to use tc for producing more monstera albo or Thai plants rather than golden pothos.
Correct! I was using a Golden Pothos in the video bc it likely will have contamination due to me talking through the entire video without the laminar flow hood on, and handling it incorrectly
Is that possible to do tissue culture on elephant foot yam?
When we select part of plant for tissue culture it's called explant not mother plant
Correct. I guess once it’s severed from the mother plant, it becomes an “explant”
Hello I got a Philodendron Pink Princess that has no grow points left. Unfortunately it would produce only pink leaves which is why that part died off and now I am left with only one initial leaf (from the cutting) and no growth point. I check the roots after 7 months of no visible new shoots. Is that plant doomed? Or is the only thing I could do to try it with tissue culture?
Hey! yes, you can induce it to produce shoots with BAP hormone. This can be done in tissue culture media, or in a normal growing mix like aroid mix
@@prettyingreen hey thanks for replying! So I just need to hormones into the soil? I have rooting hormone. I need to check if I can get my hands on some BAP Hormones.
@@ayem9504 Yup, just a shooting hormone like BAP -- hopefully it's available in your area!
@@prettyingreen ou wow ok thats great! thanks so much for this information! Ill try that to safe my pink princess… because the rootsystem looks very strong. :)
Is plant also cleaned from viruses and diseases which are inside plant and does't allow plant to give full potential? Does tissue cultured plant have bigger yield?
Possible to helpe in tissue culture, my medium get contaminated every time
How many hours light and AC must be on for tissue culture plants
Lights are usually 16H on. Temps around 76f
Do tou sell culture bottle of Albo variegated
How do I get into this for a possible future job?
Move to a country where labor is basically worthless and apply for the job.
would you not wear a mask and gloves to avoid breathing on the TC?
Absolutely wear mask. Gloves are dependent on style of technician
The only reason I Don't do TC is of weak plants. It's more like quantity vs quality. And I'm more into quality.
I’ve found TC doesn’t necessarily breed weak plants. It can at times due to somalclonal variation from repeated sub culturing, but that can also result in positive benefits too.
TC makes plants more vigorous, at least temporarily.
i think calling it the future of houseplants is a bit misleading because its been around for quite a while now(like 30+ years i think), nice video tho
True!
That is not producing laminar flow my good sir other then that great video
What is your sterile solution? I'm trying to replicate
6:14 those roots are JUICY
Yessssss 😄🤗
Interesting
😄
wow very nice video👍 from my fren farhan safuddin
so, basically the ,,you can make a new plant from every part of the plant, even from a piece of leaf" part is bs and you literaly propagate the plant by node, just on a fancy way
You can make another plant out of ANY cell of a plant. Even a single cell. It's just harder.
Beautiful🪴🌱
Take away: don't build a house with a tissue cultured tree.
Lol
That's not a laminar flow hood.
It's MY laminar flow hood: en.wikipedia.org/wiki/Laminar_flow
What he's saying is, your filter is not producing a laminar flow of air (which it's not), which is the sole purpose of a "laminar flow" hood
It's not laminar flow. Also, you shouldn't use a magnetic stirrer for sterilizing explants, the magnet can damage cells and cause somatic mutations.@@prettyingreen
1:45 You violated the area, you should never put your arms above the steril area.