i was looking for the procedure to make MS media at my lab, I could find nothing except the ingredients list, This video has helped me a lot about the procedure
it is a really good video for beginners. I want to start tissue culture on Banana. please can you inform the required media? i want to purchase ready-made media from himedia company. what should I ask them for that particular material?
Is it okay if I prepare 10X macro stock solution with final volume 500ml and instead of halving the weighed salts, I just halve the pipetted stock volume for working solution?
@@kralice462 you can have any X (times) you want, then prepare the volume accordingly… As long as you calculate using dilution formula, M1V1=M2V2 correctly, it won’t be an issue ☺️
Thank you sir for a very informative video.... i hope you intertain us here for some questions in the future.. im going to invite also everyone ( my collegues ) to subscribe your videos to get notification.. thank you once again
sir can you make a video teaching banana tissue culture, making culture media from four stages initiation media, multiplication media, elongation media and rooting media. I'd like to know the chemicals composition of that sir. please help thank you so much in advance. Godbless
Prajwal dongare to prepare 200mL stock, you need to divide the amount of salt needed to prepare stock solutions by 5... (1000mL/200mL)= 5 So divide them by 5 I do hope you know how to divide based on my examples... If there’s still confusion in it, you may use 250mL as stated in this video...it doesn’t make much difference in weight 😉
Hiii im big fan of ur voice and ur my friend excellent explanation reply pizzz
@@SubhaGeorge-wf7hn thank you!
@Plantastic12345 let's be friends
Thank you so much for uploading! Excellent video and excellent English. You’ve got a new sub for sure.
A A thanks for supporting! Please share to your friends too! More are preparing and coming
Great video & very clear
i was looking for the procedure to make MS media at my lab, I could find nothing except the ingredients list, This video has helped me a lot about the procedure
Great that this helped!
Hongs on intellectual beast mode. Best TC informative video on TH-cam
Thank you!
Thank you for your very useful inforation
No problem!
Thanks alot, it is excellent video👏👏
Thank you so much ...Sir
Beautiful video.. helps a lot 😊
Thanks! Please subsribe the channel if you found it useful~
🎉🎉
Thankyou so much ❤️sir
Thanks! I do hope it helps! Please subscribe and share! =)
How many ML to each culture dish?
Thank you have you ever use hydroponic nutrients. I just want to minimize the spend
Theoretically, you may use it, but, you need to adjust the concentration to tissue culture level...
@@Plantastic12345 thank you very much
it is a really good video for beginners. I want to start tissue culture on Banana. please can you inform the required media? i want to purchase ready-made media from himedia company. what should I ask them for that particular material?
I think you could refer to any journals on banana you want, then purchase ready MS media and add the hormone respectively
Is it okay if I prepare 10X macro stock solution with final volume 500ml and instead of halving the weighed salts, I just halve the pipetted stock volume for working solution?
@@kralice462 you can have any X (times) you want, then prepare the volume accordingly…
As long as you calculate using dilution formula, M1V1=M2V2 correctly, it won’t be an issue ☺️
10ml of stock solution @ x100 = 1L of working MS media?
Yes, if you prepared it at 100x, then you use only 10mL...
For other concentrations, you need to use dilution formula to calculate~
Thank you sir for a very informative video.... i hope you intertain us here for some questions in the future.. im going to invite also everyone ( my collegues ) to subscribe your videos to get notification.. thank you once again
Thanks for supporting!
I'm thinking Q&A series one day...
Will definitely do it! thank you!
@@Plantastic12345 it
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sir can you make a video teaching banana tissue culture, making culture media from four stages initiation media, multiplication media, elongation media and rooting media. I'd like to know the chemicals composition of that sir. please help thank you so much in advance. Godbless
Bryan Bornilla sure! Will do that when I got more subscribers more than 2k...
I’m now making videos for other tissue culture like bamboo etc
@@Plantastic12345 w0w so active I got replied lol thank you so much it will reach 2000 subs someday because u r so kind sir thank you so much
Bryan Bornilla thank you! Any request you may just let me know 😉
Bryan Bornilla currently I’m busy with other stuff...I will try my best to make more contents 😉 stay tuned!
Stock solution for 200 ml sir ?
Prajwal dongare it depends...it can be 1L, 500 mL, or 250mL...please look at 3:17...
200 ml also possible
Yes sir . Thanks but for greeting 200 ml from what number we should divide. Please help sir?
Prajwal dongare to prepare 200mL stock, you need to divide the amount of salt needed to prepare stock solutions by 5...
(1000mL/200mL)= 5
So divide them by 5
I do hope you know how to divide based on my examples...
If there’s still confusion in it, you may use 250mL as stated in this video...it doesn’t make much difference in weight 😉
Thank u sir. Great interaction with you. And best video .
Prajwal dongare if you wish to know further about the preparation, please follow us at IG and FB @Plantastic...I will share your questions
Are you a Russian Scientist? You sounded like a Russian .....
Oh, is it? hahahahhahaha....I never have the chance to communicate with Russian scientist yet...
@@Plantastic12345 if you're not a Russian, you're an Italian?
@@rjjr.1071 I'm Malaysian =)
@@Plantastic12345 wow.....but you sounded like a Russian......