DNA Sequencing By Sanger Method

This Video Explains DNA Sequencing - Sanger Method.
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[@Braindeadsoda]

It is safe to say that this is the clearest video about Sanger Method I've found on TH-cam by far, thanks for your work!

[@EasyPeasyLearning]

You are welcome 😊

[@fairooz3986]

I couldn't agree more.

[@raanamangrio322]

Exactly same words by me

[@pamom100]

100%

[@PomegranateDroid]

Lady, you just rescued my Biochemistry grade. Much appreciation.

[@EasyPeasyLearning]

Thank you 😊

[@ahmadabdullah2997]

God bless you I watched 3 lectures but could not understand the last part you made it clear
Thank you❤

[@EasyPeasyLearning]

You are welcome 🤗

[@thiagoamorim9161]

Omg, i love u, my apresentarion of Master's degree is tomorrow and I was confused on this topic. You save me

[@EasyPeasyLearning]

Thank you 😊

[@user-cp1kz4gp9k]

To the point video ever found

[@EasyPeasyLearning]

Thank you 😊

[@waterswater6544]

Needed this last year): but thank u for finally understanding this😍😍😍

[@EasyPeasyLearning]

You are welcome 😊

[@jackwining1928]

beautiful video, I'm currently doing my masters in Clinical immunology and was stuck on Sanger DNA method. THANK YOU

[@EasyPeasyLearning]

You are welcome 😊

[@bryanstapleton311]

Im not sure if Im thinking about this right, but basically, since we can differentiate between fragment sizes based on the electrophoresis (assuming the gel can differentiate sizes of fragments by just 1 base pair), we can determine a sequence simply by analyzing where each fragment migrated in each tube based on the ddNTP?
If that is correct, then how do we confirm the starting base pair of the sequence? I get that it would be the smallest fragment on the gel, therefore the band that traveled furthest, but that band can be a fragment that has 1 dNTP before the ddNTP, so that wouldn't tell us the very 1st base pair of the sequence necessarily.
So in other words, how do we know that the smallest fragment had a ddNTP at the first base pair after the primer?
Im not sure if that question makes since. But great video anyway, really clear.

[@raanamangrio322]

Best explained

[@EasyPeasyLearning]

Thank you 😊

[@mohammedal-hammadi5085]

Thank you for the informative videos

[@EasyPeasyLearning]

You are welcome dear

[@ruvimbomahewu3925]

Thank you

[@EasyPeasyLearning]

You are welcome 🤗

[@fanfan9768]

How to calculate the number of fragments with different lengths? From short to long are N (Number of DNA template) x p (Termination probability), N x (1-p)*p ... ? Is it correct? why the band in the real gel is not like this?

[@mehmethanarslan8829]

Thank you thank you thank uuuuu

[@EasyPeasyLearning]

You are welcome 😊

[@spiritedzee1158]

My Goly, I get it!

[@EasyPeasyLearning]

That's great thank you 😊

[@khalidaamir2046]

Very nice

[@EasyPeasyLearning]

Thank you

[@Jcastillo0125]

Finally I get it haha

[@coldsparks1662]

3:13 "when I added all those things in it" 😭😭 what things ?? please avoid saying vague THINGS like that . throughout the video, there were a couple vague words you used like that. Otherwise, the topic would have been clear .

[@dusk0135]

Thanks!!!! 🤍🤍🤍🤍🤍💗

[@EasyPeasyLearning]

You are welcome 😊