Hitting the *LIKE* and dropping a *COMMENT* on a Mycophilia video has been known to increase flushing power to the MAX! It's true! 🎉🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄👽🛸 1) Patreon Replacement (memberships for videos and monthly genetics): tinyurl.com/mycosage 2) Mentorship and Genetics, I don't bite!: mycophilia.official@gmail.com 3) Discount codes in description 🍄💜 Sage
Hey sage quick question I’ve recently had a problem where my jars colonize everywhere except the tops of the grain they just reach out there and seem to stall any idea on why this might be? And is there anything I can do to prevent it from happening again thank you very much very grateful and thankful for your videos 🤝💙
Duuuude!!! Just struck GOLD finding this video!!! Been struggling with bacterial riders for a whiiiile now. Basically have narrowed it down to my LFH filter been saturated, and every "open jar" agar/grain transfer I do in front of it gets bacteria that I later transfer to tubs and get Trich. Really frustrating cuz the myc looks "OK" but at the end of the cycle, all that effort and resources are lost. But, looking at your video I've learned that they may ALSO be embedded bacteria coming in the genetics purchased. 🤔 Cheers man! Keep doing these videos! One about possible origins of such contams/bacteria would be nice!!!
Thanks man, talk about serendipity. I’m currently editing a video where I briefly talk about one of the sources of such contams. Should be coming out today!
The jar that won't break-up is really good info! If your mycelium doesn't break up easily then you're going to have problems. Took me a while to figure that one out.
@@DARKW1ZARD616 I've personally never had this issue it just looks overly colonized the me but that's not a problem in my experience. I'm thinking I'm maybe not seeing the texture he's trying to show off
@@DARKW1ZARD616 Seems like the jar needed to be 'shook more' before turning too 'rubbery/solid'. It appears the yellow color is just the isolation of existing bacteria, by sitting too long.. But still; if the majority is white, it seems to need a literal breaking of the jar and salvaging the majority into at least a chance of some propriety.
Ah man, My first grow I did 10 jars and I didn’t get one that was completely clean. I was able to salvage enough to do one tub of gt And it worked. Got a bunch of really small ones the first flush, I was so happy it worked. It was mss straight to grain and I wasn’t very good at hydrating the popcorn at all. Plus I don’t think I did even a full 90 mins.
Use water agar. Or cut out a channel in your agar so the mycelium can escape the bacteria by jumping the chasm. Cleaned some s.n.a.p.e culture up via these two methods in one transfer
@@Mycophiliahe means embedded bacteria. Basically youd leave 1/3 of the dish with the bacteria embedded mycellium then empty the rest out and fill with fresh agar alongside that wedge and then cut a "ditch" out of the agar so there is a line of clear space between what's already colonized with the bacterial mycelium and the side with the fresh agar. The mycelium will walk over that gap but the bacteria in theory wouldn't transfer along with it. I haven't personally had to do this, but it certainly does make a lot of sense
@@MCH400 Yeah it's called the "trenching" method, and I haven't tried it myself either (it wasn't a thing when I made this video). Plan on trying the next time I see an issue with embedded contam 👍
I find Trich has a greyish hue, colonises very quickly and looks like cotton balls, but mostly it doesn’t have the beautiful alabaster white shiny complexion of mycelium.
I'd love a video on how to use a microscope to verify grain spawn. I've seen very few 'scopes while perusing TH-cam myco tutorials. Also, thank you for the tip regarding pressure cooking contaminated grains. I've been driving a mile away to dump, still obviously bringing spores home on my clothes.
i learned something new , ive had this . at the very leading edge of the mycelium on agar you can see a faint hays . i have a fruit on the petri dish , so im going to collect the cap and make a new spore print .
Thanks for this content, its hard to find information on hidden contamination, but it's quite common for me! One idea I wanna share with you @Mycophilia: I remember Mycoplasma are smaller then other bacteria and prefer to stay intracellular, not intercellular. Maybe they are your hidden embedded contamination. Did you try growing this kind of contaminated mycelium on Agar-water? all the best!
If your bulk is going bad then you have 1) Not fully sterilized (longer higher temps required) Why does this happen???? Elevation has an effect on temp. 2) You introduced a contaminant (Lysol/alcohol and draft hood) 3) Your inoculate is contaminated (do a better job on sterilizing and use smaller wide mouth jars)
That explains a lot. I have to cut my spawn out of the jars. There's no breaking it up. All my agar looks great ! At T -7 now starting to get ropey but put to grain it stalls out . If I shake it, it doesn't recover. Time to top fruit for some new spores. Thanks !
Glad I found your channel. I have a year old Oyster jar of spawn that is tightly grown in and has several tablespoons of liquid in it. It was pushed to the back of the fridge and forgotten. I'm gonna give it a shot, thanks for the info it looks like it might not be contam after all.
Thank you for an informative video as well. Super cool that we are all teaching each other. It won’t be a formal science in my lifetime but maybe in yours humanity will see a school of mycology on the same level as other sciences have.
Trich kinda takes over in growth quickly, if it's spotting light green it's penicillium, trich spreads much faster and has a forest green color when matured, penicillium takes longer so you can let it go a little longer before you have to toss it out, gives you chance to get some fruits out if you're at that point that is. Love the vids brotha!!! Keep fighting the good fight!!! 🦍🙏💪🏿
@@nattybizzle depends how contaminated I guess lol, you mean like if you picked fruits from a tub that was contaminated? Or do you mean if the fruit itself has contamination on it? Either way best case scenario is you get diarrhea, worst case is you get sick and die lol but i would use the fruit from a contaminated grow to try to clean up into a healthy culture for future purposes if that's what my objective was at the time.
Bro... You. Are. A. MAVERICK. thank you so much🙏. This has been EXTREMELY enlightening. I thought I had been making my grain too wet at times, but it was just the bacteria bacteriaing... That slimy grain usually smells like a jock strap, or fermented fruit. Putting it in lower temps can sometimes negate the viability of the contaminant. I've been inadvertantly experimenting with it for years. Thanks again for your insight. Very helpful!
I loved the video, but I am not really sure what to look for. I see the little yellow metabolites, and the fluffy white jars definitely look strange. But I think I just need to do more research on contam issues to get a better free asp of the possible mycelium pitfalls that you’re likely to encounter, compared to healthy growth.
After a few grows you will begin to see what healthy mycelium looks like and unhealthy mycelium looks like. It’ll all fit together so have fun while you’re at it!
I'm in the process of building a professional lab, so if you want to keep me in mind towards the end of 2023, I can run some tests on that one spawn jar that you can't identify the mold/foreign spores that keeps ruining your grow
Very interesting, but what makes you think the jars with the white mycelium are contaminated with bacteria, or even contaminated at all? Have you done some tests that support that notion? Might not something else actually be going on? (BTW, next time why don't you slice the white mycelial mass into 1.5-2 cm 'breadslices' with a knife and try putting them btw a thin 50-50 CC-Verm bottom and thicker 50-50 CC-verm top layer and see what happens. I suspect breaking up spawn that looks like that might not work very well.)
They are 💯 contaminated, once you’ve grown for some time you know what’s contaminated and what’s not. I have listed a number of reasons on this video, the primary ones being the extremely thick growth that makes it impossible to break up, along with all previous attempts with this culture always ending up with the same green mold, at least those that have been spawned to bulk. The ones that have not (bottle fruited), have not fruited in 2+ months of fruiting conditions, in addition to growing very strange mycelium masses after the first month, a hallmark of contamination. As for your idea of slicing, a better method exists called jar/top fruiting. It is better because you don’t need to break up the mycelium at all. Hope that helps.
Thanks for the video. Very informative and helpful. I appreciate it Im a beginner and having lots of trouble and videos like this one is invaluable for me
Every jar I've ever tried to spawn has turned into those thick bacterial contam but fully colonized / smells fine jars. I've been thinking it's the WBS I've been using and/or the holes in my modified lids aren't large enough.
I had a few fully colonized jars of grain and they didn't appear to have any contamination. but there was a little bit of water in the jars after they were fully colonized. But I opened them to spawn them to bulk and they all smelled good like fresh mushrooms. Do you think they should be fine ?
I have a tub that looks like the jar you said would become trich. I also think my tub is going to turn to trich, after seeing this though I am hopeful because it's passed the time other tubs usually turn green. We will see though, probably just jinxed myself lol
Mold infects plants and fungi systematically, it has me question if some bacterial infections aren’t the same. Thank you for sharing so much information 🙏
Omg that really white mycelium is something I thought was healthy and it's true right after the first flush they contam 😭 or even during the first one and I only get two fruits
I had a box that formed hard nodes all over the surface and wouldn't fruit, never seen something like that before, smelt great tho even when I broke it up and threw it away, wonder if it was some sort of embedded bacteria, I got rid of all the grain cause I tried to fruit it 4 times and gave it a month each time no success
Use plates with multiple compartments for SN-T1 2/6. Mycelium climbs over walls to section with clean agar and there's your clean wedge, success is like 99.999%. Just be very sure not to spill agar on walls.
@@Mycophiliahave you tried water agar? The mycelium Will stretch out and look for nutrients while the bacteria won't have an ability to spread without nutrients. I've been able to cleanly transfer bacterially contaminated mycelium after one or two water agar transfers
Im in a huge battle with Trich, Using peroxide....soaking....then rinsing with water. Multiple rounds....been cleaning up alot of the green. Took me so long to spawn, gonna see this battle thru
You said "these two guys are full of truffles". What does that mean? Is that an insider term for some kind of contaminant I haven't heard of? Or have you actually discovered a way to cultivate truffles?
Your vids are great reference. Two lots of 8 jars from spore 💉, using two moisture methods and 1 control jar kept outside the control environment. Only 5 days in and trying to just leave them alone is so difficult 😂. Hopefully in a week or so I'll find out how clean ive been.
Hey! I think I'm dealing with the same bacteria as you. I've also done multiple transfers to both agar and grain and still am seeing those big fluffy clumps on top. Do you know if it's definitely bacterial, or could it be a mold or yeast? If it's bacterial then perhaps a transfer to antibiotic agar could clean it up?
Definitely worth a try. I would recommend doing at 2-3 xfers on antibiotic agar, and then 2 xfers on regular agar to make sure it won’t come back. This is because bacteria will always eventually outsmart the antibiotics eventually
Great vid man. I stopped using WBS because some bacteria can burro deep in low grade grains and can survive the pressure cooker. Keen eye on that particularly deceptive agar plate. I wouldn't have caught that one in a million years. Patches of mycelium that grow little spikes are another sign to look out for. Keep up the good work, homie.
@The Eye-Rollers Guide to the Galaxy haha just watched your vid about this! Embedded bacteria huh? Well guys live and learn...sigh. What in Your guys opinion would be the bullet proof medium to avoid all this? Thanks to both of you
Thank you! I just spawned a bacterial B+ to a shoebox with a 1:1 ratio. It looks great but I will have an eye on it. Had to scoop out the grains but they smelled wonderful still.
@@Mycophilia If you have a few bacterial spots on the outside, cant you rub the spawn until its sides are rubbed off and just bulk the middle part, which should be clean because it has its stable immune system.?
@@user-xr5cz2mr4vno, the bacteria transfers via water, humidity. Culture has to be cleaned up via water agar, or multiple transfers on regular nutrient agar with chasms sliced into the agar to impede bacterial spread
I am in the same Situation right now.. Mycelium looks really thick and a lot of White dots and uneven, not flat at all.. but no bad smell at all... I still made a bulk in a monotub, but I don't know if it works.. I have got an orange touch and some dry powder from some jars.. I assumed it to be Penicillin, but who knows!!
I wish I would have seen this yesterday. I had one jar that wasn't quite as far as the other. Went to bulk with them both....together....in the same tub. Both were full of mycelium but one just looked better. They smelled earthy. Had no reason to think they were bad in any way. Judging by your video I may have messed up. What should I look for? Is there a chance it's fine? I feel like a bad parent. These are my babies 😢
Hey great video! I am doing LC's for my tranfers to grain and it seems i get this thick white mycileum along the sides of my jars, but the center is pretty easy to break up and not thick at all. Does that sound like that bacteria or just mycelium ?
Those "embedded bacterial" jars are not actually contaminated. I think he's mistaking crappy genetics. Those don't look bad at all. There's no "tell tale" sign of anything in those jars
Ive just started out and did as much of the sterilisation I could with everything. I did an oyster batch but looked like white fuzzy mold starting after a few days so got rid of it also those random white dots in various places. Then I redid the process trying to be even more careful with another LC syringe (GT) I got in a pack. Was as sterile as i could be with alcohol, etc but on rye and oven baked at 80 deg C for about 2.5 hrs since i dont have a PC yet. Made sure to squirt down side to monitor, after 3 days I can see white either whispy or early signs of white fuzz. Should i leave it and monitor or perhaps toss it while its pretty early? Appreciate the content and learning a ton so far.
Ok ignore previous comment I watched your rhizo video and my mind is at ease now. I wish we could show pics here so you can see how my grows are coming along thanks to your advice. Keep at it love the info ❤
Apologies if you've already addressed this in a previous video, but when fruiting truffle producers, will the fruits be more potent the longer i let the spawn colonize? Or as soon as the jars is colonized(little to no truffles forming) spawning to fruit will be the same potency?
the fruits are tiny and potent as it is. Recent run was 6 months truffle then fruiting trays with peatmoss casing, but I'm going straight fruiting for Tampa / Mexi this round. Will see if they are in the same ballpark.
Small changes to variables is useful to determine a proper path ? Is this the reason one of three had different outcomes?Thanks for informative science on these fascinating life species💕❤️
It’ll help deter bacterial growth, but if the bacteria’s already ‘triggered’ then it won’t make much difference as the mycelium will always lose eventually
Question, were those white jars of the compressed myc started from what looked like a singular culture on an agar plate from spores? I think people get germinating spores mixed up at times. Spores can germinate and grow by themselves. They only need to meet up later with other germinated spores to go on and grow healthy mycelium and eventually fruit. This may be the problem with that myc. I'd try introducing another sample of myc that came from the same strain and see of they marry 🤷
After scaling up my grow I got a lot of bacterial contam, still trying to rule it out. I was using popcorn, never had a problem before while using jars, but when I moved to bags I got into problems, all of my contams are bacterial, not a single colorful mold :( Now im using millet, perhaps it will be easier to achieve clean spawn.
I would never thought that unbreakable mycelium is contaminated...I am no expert tho.... maybe anti bacterial agar would help... make clean coulture...?
What exactly the smell end up like if that's not contaminated? I've done my first try, the mycelium bottle smelled like wine, quite strong when breathing in, suppose I should wear mask before opening the lid
Hey I had a grain bag w some wet rot and a lot of v healthy mycelium. I mixed it into substrate and it did smell for a bit but now the smell is gone and the cake looks amazing. Should I be worried about the fruits having some toxins from the wet rot? Can the mycelium eat the bacillus and any toxins they would have thrown? Any tips on what to do w the fruits? I have a feeling they are going to “look” v healthy and don’t want to waste the bag I’ve had a hard time salvaging this strain! Thank u!!!
I started doing this chasing mycelium into fruit about a year ago and I use the uncle Ben’s method but the last six syringes I received all turned into super green nasty. My guess is that it’s Trichoderma has a very foul smell to it. my first I’d say eight times was very successful. I have not changed my procedures if anything I have gotten even more excessive compulsive about cleanliness. I am guessing that I am getting contaminated syringes. From your experience, is that a possibility?
Why one would bother with PF tek anymore is beyond me. But each to there own. First I had to pressure cook the jars, drill holes then squirt 2ml of liquid spawn into each jar. I did 4 jars, lost two cakes to bacteria, then put the other two in a tub with perlite on foil. (It took a month to colonise the jars btw). One cake produced a few fruits then that was it. I’ll never do it again.
Thanks for the good oil! i have a similar situation with a H.PES genetic... i was sure its carrying something, but i cant see it on agar. there is a new tek available that is being tested in the community btw. Rit dye agar.... the other option would perhaps be water agar ?
@@Animal-Reaction-Clips lmao you’re one of those morons. Okay then explain how one would “keep it simple” when their cultures are contaminated? Dirty straight from the vendor?
It should, I would recommend doing 2-3 xfers on antibiotic bacteria, then 2 transfers on normal agar to make sure it doesn’t come back (as bacteria will always overcome the antibiotics eventually, so you need to make sure they’re gone for sure before putting it to grain)
So what if your spawn bag had some wet rot maybe not wet rot but a few slimy spots in the beginning but then the mycelium still took over and properly spawned? Is it still good to use? I went ahead and put it in a montotub and it looks like it’s colonizing properly
Because water agar does nothing to embedded contams as the mycelium itself is contaminated, it’s not something you can easily isolate like regular bacteria via water agar.
This would be the first I have heard of “embedded” bacteria that can not be removed. Can you kill it with antibiotics? It seems like you’re suggesting that there is a bacteria that is living and feeding from the mycelium it’s self, definitely seems plausible.
Super helpful video thank you so much! I've heard that the "infused bacteria" in the mycelium can be a sign of a bad syringe, can anyone confirm if this is true?
Not necessarily a sign of a bad syringe as you can get it thru any medium. But dirty spores will greatly increase likelihood of getting contaminated mycelium.
Is this all you got? lol. I was hoping to get nightmares from a mutant Blob consuming the city. I grow Tric just for fun (or so I tell myself). Sorry but my contaminants would eat yours for breakfast. Aspergrillis is my homeboy. It's not a grow till I see happy knats greeting me! But seriously folks... Good stuff. I see wet rot too often in my oat grain jars. Thanks for good info!!
have you added ashes just wanting to know if your hypothesis works ? i personally wanna try those jelly-looking colored balls that you soak in water till they become the size of a marble after 24 hrs later. people use them inplace of soil to grow most houseplants anyway i was thinking just adding a few dry balls when they are the same size of rice and it works just like white rice to suck the moisture out of your cell phone. like a silica packet does.idk ill let you know how it works 🙂
@@Lexington-Felix orbees only hold moisture, and you have to use nutrients to keep a plant alive. Along with frequent replacement of the spheres of polymer and water. Mushrooms are very hard to grow outside of their natural environment. In nature they can survive other molds and bacteria. In the lab they require a sterile environment.
So i am letting my agar plates grow . I plan to transfer them to jars and substrate in a tub . My question is i just recently noticed that my ceiling has a few black colored mould on the ceiling .if im careful and do my air transfer outsude is it ok to grow inside of a tub if there is some mould on the ceiling ? Is there anything i should or shouldn't do ? This is my first time growing . Please and thank you
Where can I get ahold of You, personally? I've got some Grains I'd like You to see so You can possibly help me decide on what it is & the nxt steps I'll be needin to take. Also, THANK YOU for all the GREAT ADVICE & KNOWLEDGE that You KINDLY SHARE w ALL OF US!!! 🤙🏽😎🤝🏽
Hey 1T thanks for watching and commenting! I currently only do private questions in my mentorship program. Otherwise you can post your questions on my subreddit or discord server (you can tag me with your picture with @mycosage and I will come take a look).
Heey homie i Had nothing but problems with fliped lid tek!!! 😞 Switched to ball brand plastic lids with 1 , 1/4 inch hole , covered with mirco pore tape i use 2 layers! Grab some low nutrient antibiotic agar if ya haven't already those plates will clean up 🔥🤙 🍄
Nothing to do with flipped lid here. But I appreciate your comment nonetheless. Antibiotic agar is also not a cure all. Most times, it will only accomplish suppressing the contamination temporarily, but once put to grain or a non-antibiotic plate, the bacteria will be back.
I'm on my first attempt, and at first, the mycelium was growing beautifully, but today, I noticed a spot of localised green mould near the top of the jar. What would be your advice? It hasn't spread yet, and it would be easy enough to scoop it out with a sterilised spoon and discard, but I'm worried that the whole lot might need to be thrown out.
I just got some golden teacher. And when I injected my bag, it looks very wet like this. Did I put to much? Do I have mold? Bacteria?? It's been 5 days.
![[#2024MAMA] BIGBANG (빅뱅) - 뱅뱅뱅 (BANG BANG BANG) + FANTASTIC BABY | Mnet 241123 방송](http://i.ytimg.com/vi/EQsYfiPR9uM/mqdefault.jpg)
Hitting the *LIKE* and dropping a *COMMENT* on a Mycophilia video has been known to increase flushing power to the MAX! It's true! 🎉🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄👽🛸
1) Patreon Replacement (memberships for videos and monthly genetics): tinyurl.com/mycosage
2) Mentorship and Genetics, I don't bite!: mycophilia.official@gmail.com
3) Discount codes in description
🍄💜
Sage
I think I'm just going to clean all my stuff out start fresh with a new spore syringe it's been awhile I need to get some fruits
Hey sage quick question I’ve recently had a problem where my jars colonize everywhere except the tops of the grain they just reach out there and seem to stall any idea on why this might be? And is there anything I can do to prevent it from happening again thank you very much very grateful and thankful for your videos 🤝💙
Great vid
Hi sage, I need grain spawn bags, do you have a referral? Thanks
your Canadian?
eh
🍁
Duuuude!!! Just struck GOLD finding this video!!! Been struggling with bacterial riders for a whiiiile now. Basically have narrowed it down to my LFH filter been saturated, and every "open jar" agar/grain transfer I do in front of it gets bacteria that I later transfer to tubs and get Trich. Really frustrating cuz the myc looks "OK" but at the end of the cycle, all that effort and resources are lost. But, looking at your video I've learned that they may ALSO be embedded bacteria coming in the genetics purchased. 🤔 Cheers man! Keep doing these videos! One about possible origins of such contams/bacteria would be nice!!!
Thanks man, talk about serendipity. I’m currently editing a video where I briefly talk about one of the sources of such contams. Should be coming out today!
Good shout bless you and your family
The jar that won't break-up is really good info! If your mycelium doesn't break up easily then you're going to have problems. Took me a while to figure that one out.
I recently broke up couple spawn bags all of them were kinda hard should I be concerned?
Thanks for sharing this information . Bless you and your family
@@DARKW1ZARD616 I've personally never had this issue it just looks overly colonized the me but that's not a problem in my experience. I'm thinking I'm maybe not seeing the texture he's trying to show off
@@DARKW1ZARD616 Seems like the jar needed to be 'shook more' before turning too 'rubbery/solid'. It appears the yellow color is just the isolation of existing bacteria, by sitting too long.. But still; if the majority is white, it seems to need a literal breaking of the jar and salvaging the majority into at least a chance of some propriety.
Ah man, My first grow I did 10 jars and I didn’t get one that was completely clean. I was able to salvage enough to do one tub of gt And it worked. Got a bunch of really small ones the first flush, I was so happy it worked. It was mss straight to grain and I wasn’t very good at hydrating the popcorn at all. Plus I don’t think I did even a full 90 mins.
Use water agar.
Or cut out a channel in your agar so the mycelium can escape the bacteria by jumping the chasm.
Cleaned some s.n.a.p.e culture up via these two methods in one transfer
You were able to clean up embedded bacteria with water agar? Or regular bacteria?
That's a phenomenal idea actually. Smart. The mycelium will reach across while the bacteria is held on the contaminated side 🎉🎉🎉🎉
@@Mycophiliahe means embedded bacteria. Basically youd leave 1/3 of the dish with the bacteria embedded mycellium then empty the rest out and fill with fresh agar alongside that wedge and then cut a "ditch" out of the agar so there is a line of clear space between what's already colonized with the bacterial mycelium and the side with the fresh agar. The mycelium will walk over that gap but the bacteria in theory wouldn't transfer along with it. I haven't personally had to do this, but it certainly does make a lot of sense
@@MCH400 Yeah it's called the "trenching" method, and I haven't tried it myself either (it wasn't a thing when I made this video). Plan on trying the next time I see an issue with embedded contam 👍
I find Trich has a greyish hue, colonises very quickly and looks like cotton balls, but mostly it doesn’t have the beautiful alabaster white shiny complexion of mycelium.
A lot of trich is gray, but they also go super white just as much. Waaaay whiter than mycelium, usually before turning green.
I'd love a video on how to use a microscope to verify grain spawn. I've seen very few 'scopes while perusing TH-cam myco tutorials. Also, thank you for the tip regarding pressure cooking contaminated grains. I've been driving a mile away to dump, still obviously bringing spores home on my clothes.
i learned something new , ive had this . at the very leading edge of the mycelium on agar you can see a faint hays . i have a fruit on the petri dish , so im going to collect the cap and make a new spore print .
This video has answered so many questions for me and new avenues to pursue which I thought were ended, thank you for this
Did it scare you away or make you spend 250$ on cleaning supplies and protection ?
my fav youtube chanel so far
💜 thanks 🍄
Thanks for this content, its hard to find information on hidden contamination, but it's quite common for me!
One idea I wanna share with you @Mycophilia: I remember Mycoplasma are smaller then other bacteria and prefer to stay intracellular, not intercellular. Maybe they are your hidden embedded contamination.
Did you try growing this kind of contaminated mycelium on Agar-water?
all the best!
Certainly could be the case. As for water agar, yes, and ime it does nothing for embedded contams unfortunately
If your bulk is going bad then you have
1) Not fully sterilized (longer higher temps required) Why does this happen???? Elevation has an effect on temp.
2) You introduced a contaminant (Lysol/alcohol and draft hood)
3) Your inoculate is contaminated (do a better job on sterilizing and use smaller wide mouth jars)
It’s 3, I cover that in the video. Thanks for the comment!
That explains a lot. I have to cut my spawn out of the jars. There's no breaking it up. All my agar looks great ! At T -7 now starting to get ropey but put to grain it stalls out . If I shake it, it doesn't recover. Time to top fruit for some new spores. Thanks !
🍀 💜
7 transfers?
I had no idea that the mycelium itself could be contaminated. I had this problem for many runs and took a break because of it.
Something that isn’t talked about enough!
Agreed! @@Mycophilia
Glad I found your channel. I have a year old Oyster jar of spawn that is tightly grown in and has several tablespoons of liquid in it. It was pushed to the back of the fridge and forgotten. I'm gonna give it a shot, thanks for the info it looks like it might not be contam after all.
That sounds like bacteria to me but I’d give it a shot anyways!
Glad you found my channel, hope you enjoy!
Thank you for an informative video as well. Super cool that we are all teaching each other. It won’t be a formal science in my lifetime but maybe in yours humanity will see a school of mycology on the same level as other sciences have.
Trich kinda takes over in growth quickly, if it's spotting light green it's penicillium, trich spreads much faster and has a forest green color when matured, penicillium takes longer so you can let it go a little longer before you have to toss it out, gives you chance to get some fruits out if you're at that point that is. Love the vids brotha!!! Keep fighting the good fight!!! 🦍🙏💪🏿
👍 👍
You can eat contamed fruit?
@@nattybizzle depends how contaminated I guess lol, you mean like if you picked fruits from a tub that was contaminated? Or do you mean if the fruit itself has contamination on it? Either way best case scenario is you get diarrhea, worst case is you get sick and die lol but i would use the fruit from a contaminated grow to try to clean up into a healthy culture for future purposes if that's what my objective was at the time.
Bro... You. Are. A. MAVERICK. thank you so much🙏. This has been EXTREMELY enlightening. I thought I had been making my grain too wet at times, but it was just the bacteria bacteriaing... That slimy grain usually smells like a jock strap, or fermented fruit. Putting it in lower temps can sometimes negate the viability of the contaminant. I've been inadvertantly experimenting with it for years. Thanks again for your insight. Very helpful!
I loved the video, but I am not really sure what to look for. I see the little yellow metabolites, and the fluffy white jars definitely look strange. But I think I just need to do more research on contam issues to get a better free asp of the possible mycelium pitfalls that you’re likely to encounter, compared to healthy growth.
After a few grows you will begin to see what healthy mycelium looks like and unhealthy mycelium looks like. It’ll all fit together so have fun while you’re at it!
I'm in the process of building a professional lab, so if you want to keep me in mind towards the end of 2023, I can run some tests on that one spawn jar that you can't identify the mold/foreign spores that keeps ruining your grow
FBI
What is blud wafflin about
Have u tried blastinguvc sterilizing light myco survives the bactia is bleached works best on colonized agar and liquid culture
@@JamesButler-j5f what is the tech that you use for the UVC light?
Very interesting, but what makes you think the jars with the white mycelium are contaminated with bacteria, or even contaminated at all? Have you done some tests that support that notion? Might not something else actually be going on? (BTW, next time why don't you slice the white mycelial mass into 1.5-2 cm 'breadslices' with a knife and try putting them btw a thin 50-50 CC-Verm bottom and thicker 50-50 CC-verm top layer and see what happens. I suspect breaking up spawn that looks like that might not work very well.)
They are 💯 contaminated, once you’ve grown for some time you know what’s contaminated and what’s not. I have listed a number of reasons on this video, the primary ones being the extremely thick growth that makes it impossible to break up, along with all previous attempts with this culture always ending up with the same green mold, at least those that have been spawned to bulk. The ones that have not (bottle fruited), have not fruited in 2+ months of fruiting conditions, in addition to growing very strange mycelium masses after the first month, a hallmark of contamination.
As for your idea of slicing, a better method exists called jar/top fruiting. It is better because you don’t need to break up the mycelium at all. Hope that helps.
By the smell lololol
@Enochulate88 he said it didn't have the smell.
@@Mycophilia were you ever able to get bottle fruits from these or did you end up scrapping them?
@@Alexmw777 never fruited after like 2 months
Thanks for the video. Very informative and helpful. I appreciate it Im a beginner and having lots of trouble and videos like this one is invaluable for me
Glad it was helpful!
THANK YOU!!! This was very informative.
Great video! Now I know why I keep getting failures with my latest batches. Time to go back to spore.
Thanks Melissa for the comment!
How do you recommend disposing of contaminated spawn? Thanks for the great content.
th-cam.com/video/FJmXVLYp954/w-d-xo.html
Chuck in in your neighbour dish
Every jar I've ever tried to spawn has turned into those thick bacterial contam but fully colonized / smells fine jars. I've been thinking it's the WBS I've been using and/or the holes in my modified lids aren't large enough.
You should see how small premade holes are. Like a screw driver or nail size
You should see how small premade holes are. Like a screw driver or nail size
I had a few fully colonized jars of grain and they didn't appear to have any contamination. but there was a little bit of water in the jars after they were fully colonized. But I opened them to spawn them to bulk and they all smelled good like fresh mushrooms. Do you think they should be fine ?
Probably, only so much I have to go on with that description but I’d definitely spawn it either way
Thank you for putting this together.
My pleasure Hudman, glad you enjoy!
One day I'll be ready for this
I have a tub that looks like the jar you said would become trich. I also think my tub is going to turn to trich, after seeing this though I am hopeful because it's passed the time other tubs usually turn green. We will see though, probably just jinxed myself lol
Love this video content. Very helpful. Thank you so much! Subscribed!
Thank you 😊
Finally a video with good information.
Cheers thanks for your comment 😊
Mold infects plants and fungi systematically, it has me question if some bacterial infections aren’t the same.
Thank you for sharing so much information 🙏
Thank you for watching :)
Omg that really white mycelium is something I thought was healthy and it's true right after the first flush they contam 😭 or even during the first one and I only get two fruits
I dig your channel man...doing gods work
Thank you 😊
I had a box that formed hard nodes all over the surface and wouldn't fruit, never seen something like that before, smelt great tho even when I broke it up and threw it away, wonder if it was some sort of embedded bacteria, I got rid of all the grain cause I tried to fruit it 4 times and gave it a month each time no success
Use plates with multiple compartments for SN-T1 2/6. Mycelium climbs over walls to section with clean agar and there's your clean wedge, success is like 99.999%. Just be very sure not to spill agar on walls.
I like that idea for regular bacteria but with embedded bacteria like this, I can’t imagine it helping as the contam exists within the mycelium
@@Mycophilia i had same problem and climbing mycelium was the only way in my case
@@Mycophiliahave you tried water agar? The mycelium Will stretch out and look for nutrients while the bacteria won't have an ability to spread without nutrients. I've been able to cleanly transfer bacterially contaminated mycelium after one or two water agar transfers
Im in a huge battle with Trich, Using peroxide....soaking....then rinsing with water. Multiple rounds....been cleaning up alot of the green. Took me so long to spawn, gonna see this battle thru
Another banger video my brother!!!
You said "these two guys are full of truffles". What does that mean? Is that an insider term for some kind of contaminant I haven't heard of? Or have you actually discovered a way to cultivate truffles?
i think hes talking about the kind of truffles you find at headshops in ansterdam. not a new phenomenon.
Amazing video, very informative :D
Mush love
Thanks MJ 💜
Your vids are great reference. Two lots of 8 jars from spore 💉, using two moisture methods and 1 control jar kept outside the control environment. Only 5 days in and trying to just leave them alone is so difficult 😂. Hopefully in a week or so I'll find out how clean ive been.
thank you so much for this video. 🍄💙
You're welcome, glad it helped 😊
Hey! I think I'm dealing with the same bacteria as you. I've also done multiple transfers to both agar and grain and still am seeing those big fluffy clumps on top. Do you know if it's definitely bacterial, or could it be a mold or yeast? If it's bacterial then perhaps a transfer to antibiotic agar could clean it up?
Definitely worth a try. I would recommend doing at 2-3 xfers on antibiotic agar, and then 2 xfers on regular agar to make sure it won’t come back. This is because bacteria will always eventually outsmart the antibiotics eventually
It’s definitely not mold, that much I can say. Yeast is possible as well, although I can’t say for certain.
"They are like, one unit". The Cids comedown has that hitting hard in the feels.
Great vid man. I stopped using WBS because some bacteria can burro deep in low grade grains and can survive the pressure cooker. Keen eye on that particularly deceptive agar plate. I wouldn't have caught that one in a million years. Patches of mycelium that grow little spikes are another sign to look out for. Keep up the good work, homie.
Thanks, appreciate it 💜
So what is the best thing to use then? Thanks
If you soak the grains for 48 hours you can germinate the bacterial endospores and this way they will be killed in the pressure cooker.
@@consolechips Right on, Thanks. That makes perfect sense.
@The Eye-Rollers Guide to the Galaxy haha just watched your vid about this! Embedded bacteria huh? Well guys live and learn...sigh. What in Your guys opinion would be the bullet proof medium to avoid all this? Thanks to both of you
Cleared up a lot here good timing on this topic!!! Thank you so much Sage always learning more from you!
Glad it was helpful!
Thank you! I just spawned a bacterial B+ to a shoebox with a 1:1 ratio. It looks great but I will have an eye on it. Had to scoop out the grains but they smelled wonderful still.
Best of luck! 🤞
@@Mycophilia If you have a few bacterial spots on the outside, cant you rub the spawn until its sides are rubbed off and just bulk the middle part, which should be clean because it has its stable immune system.?
@@user-xr5cz2mr4vno, the bacteria transfers via water, humidity.
Culture has to be cleaned up via water agar, or multiple transfers on regular nutrient agar with chasms sliced into the agar to impede bacterial spread
I am in the same Situation right now.. Mycelium looks really thick and a lot of White dots and uneven, not flat at all.. but no bad smell at all... I still made a bulk in a monotub, but I don't know if it works.. I have got an orange touch and some dry powder from some jars.. I assumed it to be Penicillin, but who knows!!
Powder and orange are a no-go
@@Mycophilia yes, but is it Penicillin, because it has no smell. It actually smells fresh?!
@@Mycophilia the Orange is like the colour from oyster mycelium, more white, it just looks like that in the reflection, only slightly!
I wish I would have seen this yesterday. I had one jar that wasn't quite as far as the other. Went to bulk with them both....together....in the same tub. Both were full of mycelium but one just looked better. They smelled earthy. Had no reason to think they were bad in any way. Judging by your video I may have messed up. What should I look for? Is there a chance it's fine? I feel like a bad parent. These are my babies 😢
There is hope, they may be completely fine. Only one way to tell
@@Mycophilia only way out of it is through it! Full steam ahead!
Hey great video!
I am doing LC's for my tranfers to grain and it seems i get this thick white mycileum along the sides of my jars, but the center is pretty easy to break up and not thick at all. Does that sound like that bacteria or just mycelium ?
Thank you, if it’s easy to break up that is a good sign that it’s not contaminated. The only way to truly tell if it is clean is to just try using it.
Thank you for the quick response, much appreciated.
I will se how they work out 🙂
never heard of embedded bacteria, I thought if it was clean on agar it's clean period, thanks for the vid
Those "embedded bacterial" jars are not actually contaminated. I think he's mistaking crappy genetics. Those don't look bad at all. There's no "tell tale" sign of anything in those jars
Ive just started out and did as much of the sterilisation I could with everything. I did an oyster batch but looked like white fuzzy mold starting after a few days so got rid of it also those random white dots in various places. Then I redid the process trying to be even more careful with another LC syringe (GT) I got in a pack. Was as sterile as i could be with alcohol, etc but on rye and oven baked at 80 deg C for about 2.5 hrs since i dont have a PC yet. Made sure to squirt down side to monitor, after 3 days I can see white either whispy or early signs of white fuzz. Should i leave it and monitor or perhaps toss it while its pretty early? Appreciate the content and learning a ton so far.
Ok ignore previous comment I watched your rhizo video and my mind is at ease now. I wish we could show pics here so you can see how my grows are coming along thanks to your advice. Keep at it love the info ❤
Glad to hear that 😊 best of luck!
Apologies if you've already addressed this in a previous video, but when fruiting truffle producers, will the fruits be more potent the longer i let the spawn colonize? Or as soon as the jars is colonized(little to no truffles forming) spawning to fruit will be the same potency?
the fruits are tiny and potent as it is. Recent run was 6 months truffle then fruiting trays with peatmoss casing, but I'm going straight fruiting for Tampa / Mexi this round. Will see if they are in the same ballpark.
Small changes to variables is useful to determine a proper path ? Is this the reason one of three had different outcomes?Thanks for informative science on these fascinating life species💕❤️
Great video! Will keeping my grains at a slightly cooler temp help deter the bacterial growth and allow the mycelium to overtake it?
It’ll help deter bacterial growth, but if the bacteria’s already ‘triggered’ then it won’t make much difference as the mycelium will always lose eventually
I'm really glad to find this video today because I have a big tub exactly like that
Glad to hear that!
I'm when I can get a set up figured out I'm gonna look at mutations with variable mutates like bacteria uv exposer ect...
Question, were those white jars of the compressed myc started from what looked like a singular culture on an agar plate from spores?
I think people get germinating spores mixed up at times.
Spores can germinate and grow by themselves. They only need to meet up later with other germinated spores to go on and grow healthy mycelium and eventually fruit.
This may be the problem with that myc. I'd try introducing another sample of myc that came from the same strain and see of they marry 🤷
No
After scaling up my grow I got a lot of bacterial contam, still trying to rule it out. I was using popcorn, never had a problem before while using jars, but when I moved to bags I got into problems, all of my contams are bacterial, not a single colorful mold :( Now im using millet, perhaps it will be easier to achieve clean spawn.
I would never thought that unbreakable mycelium is contaminated...I am no expert tho....
maybe anti bacterial agar would help... make clean coulture...?
What exactly the smell end up like if that's not contaminated? I've done my first try, the mycelium bottle smelled like wine, quite strong when breathing in, suppose I should wear mask before opening the lid
worst is to clean the jars bacterial is fine only weird smell but the mold ew.
Excellent and informative content 🤗
Thanks Ovrjoyd 💜
I have the same problem when I put him to fruit I get a little bit but just stalls out
Hey I had a grain bag w some wet rot and a lot of v healthy mycelium. I mixed it into substrate and it did smell for a bit but now the smell is gone and the cake looks amazing. Should I be worried about the fruits having some toxins from the wet rot? Can the mycelium eat the bacillus and any toxins they would have thrown? Any tips on what to do w the fruits? I have a feeling they are going to “look” v healthy and don’t want to waste the bag I’ve had a hard time salvaging this strain! Thank u!!!
very useful info 👍 cheers Sage
Glad it was helpful!
Cheers new channel and new to growing mushrooms good info thanks
🍄 💜
Hi❤
Such a great video for a beginner Ty xoxoxo 😊
Üblicher Weise fertigt man aus dem Petrischalenansatz erst einmal Flüssigmycel an. Dadurch lassen sich viele Probleme mit Kontaminationen vermeiden.
I started doing this chasing mycelium into fruit about a year ago and I use the uncle Ben’s method but the last six syringes I received all turned into super green nasty. My guess is that it’s Trichoderma has a very foul smell to it. my first I’d say eight times was very successful. I have not changed my procedures if anything I have gotten even more excessive compulsive about cleanliness. I am guessing that I am getting contaminated syringes. From your experience, is that a possibility?
Absolutely
Why one would bother with PF tek anymore is beyond me.
But each to there own.
First I had to pressure cook the jars, drill holes then squirt 2ml of liquid spawn into each jar.
I did 4 jars, lost two cakes to bacteria, then put the other two in a tub with perlite on foil.
(It took a month to colonise the jars btw).
One cake produced a few fruits then that was it.
I’ll never do it again.
What do you do now
Thanks for the good oil! i have a similar situation with a H.PES genetic... i was sure its carrying something, but i cant see it on agar. there is a new tek available that is being tested in the community btw. Rit dye agar.... the other option would perhaps be water agar ?
I’ll make an update if anything works!
Why not just keep it simple, it's fungus it'll grow on shit if you leave it alone.
@@Animal-Reaction-Clips lmao you’re one of those morons. Okay then explain how one would “keep it simple” when their cultures are contaminated? Dirty straight from the vendor?
What if instead of breaking them up you used wide mouth jars and just bury them in substrate?
as in top-fruiting? If so I cover that in this video.
@@Mycophilia Not exactly, I was thinking of doing it in a tub.
@@Mycophilia Wide mouth just to get the cakes out easy.
I think I have the same problem. Transfer through antibiotic agar could help with those embedded bacteria (gentamicin, chloramfenicol)?
It should, I would recommend doing 2-3 xfers on antibiotic bacteria, then 2 transfers on normal agar to make sure it doesn’t come back (as bacteria will always overcome the antibiotics eventually, so you need to make sure they’re gone for sure before putting it to grain)
So what if your spawn bag had some wet rot maybe not wet rot but a few slimy spots in the beginning but then the mycelium still took over and properly spawned? Is it still good to use? I went ahead and put it in a montotub and it looks like it’s colonizing properly
Let me say it better I did too much syringe and it was a little wet took a few weeks to colonize but it did so is it still safe * newbie
It’s not an automatic death sentence, fruits aren’t guaranteed but with wet rot you can oftentimes get a flush or two before trich takes over.
How do you dispose it, throw the jars out, or can they be reused?
I got a vid on that:
th-cam.com/video/FJmXVLYp954/w-d-xo.htmlsi=SBJ3nBrx3mI7vhx9
Im curious why don't you use water agar to isolate the Mycelium from the bacteria ???
Because water agar does nothing to embedded contams as the mycelium itself is contaminated, it’s not something you can easily isolate like regular bacteria via water agar.
This would be the first I have heard of “embedded” bacteria that can not be removed. Can you kill it with antibiotics? It seems like you’re suggesting that there is a bacteria that is living and feeding from the mycelium it’s self, definitely seems plausible.
Bro❤❤❤❤ i fucking love yer plates😊 absolutely gorgeous
I always run into the bottom of my jars getting wet mold.idk if it pack the grain to tight or what
Very informative, thank you!
Super helpful video thank you so much! I've heard that the "infused bacteria" in the mycelium can be a sign of a bad syringe, can anyone confirm if this is true?
Not necessarily a sign of a bad syringe as you can get it thru any medium. But dirty spores will greatly increase likelihood of getting contaminated mycelium.
Those overlaying myceliums often look quite funky such as the ps. Nat
I got some real overlay on a mckenaii tub
How did the top fruiting jar do? I have an identical situation going on here 😂
Nothing after months unfortunately, the contam was too strong. Hope you have better results :)
Is this all you got? lol. I was hoping to get nightmares from a mutant Blob consuming the city. I grow Tric just for fun (or so I tell myself). Sorry but my contaminants would eat yours for breakfast. Aspergrillis is my homeboy. It's not a grow till I see happy knats greeting me!
But seriously folks... Good stuff. I see wet rot too often in my oat grain jars.
Thanks for good info!!
Oh no, not the aspergillus! Thanks for the comment Beekeeper!
Steam your jars longer, or test out adding ashes. Some mushrooms bloom best after a fire
have you added ashes just wanting to know if your hypothesis works ? i personally wanna try those jelly-looking colored balls that you soak in water till they become the size of a marble after 24 hrs later. people use them inplace of soil to grow most houseplants anyway i was thinking just adding a few dry balls when they are the same size of rice and it works just like white rice to suck the moisture out of your cell phone. like a silica packet does.idk ill let you know how it works 🙂
@@Lexington-Felix orbees only hold moisture, and you have to use nutrients to keep a plant alive. Along with frequent replacement of the spheres of polymer and water. Mushrooms are very hard to grow outside of their natural environment. In nature they can survive other molds and bacteria. In the lab they require a sterile environment.
So i am letting my agar plates grow . I plan to transfer them to jars and substrate in a tub . My question is i just recently noticed that my ceiling has a few black colored mould on the ceiling .if im careful and do my air transfer outsude is it ok to grow inside of a tub if there is some mould on the ceiling ? Is there anything i should or shouldn't do ? This is my first time growing . Please and thank you
Awesome video man.
Thanks Steven 💜
Great video ❤
Where can I get ahold of You, personally? I've got some Grains I'd like You to see so You can possibly help me decide on what it is & the nxt steps I'll be needin to take. Also, THANK YOU for all the GREAT ADVICE & KNOWLEDGE that You KINDLY SHARE w ALL OF US!!! 🤙🏽😎🤝🏽
Hey 1T thanks for watching and commenting! I currently only do private questions in my mentorship program. Otherwise you can post your questions on my subreddit or discord server (you can tag me with your picture with @mycosage and I will come take a look).
@@Mycophilia Okay, cool. Thank you!
Where can I get the truffle culture and how it's called?
Awesome video very helpful thanks bro
Thanks for the comment, glad you found it helpful Mr. Pro!
Heey homie i Had nothing but problems with fliped lid tek!!! 😞 Switched to ball brand plastic lids with 1 , 1/4 inch hole , covered with mirco pore tape i use 2 layers! Grab some low nutrient antibiotic agar if ya haven't already those plates will clean up 🔥🤙 🍄
Nothing to do with flipped lid here. But I appreciate your comment nonetheless. Antibiotic agar is also not a cure all. Most times, it will only accomplish suppressing the contamination temporarily, but once put to grain or a non-antibiotic plate, the bacteria will be back.
interesting I've probably come across that without realizing what it was.
Are you going to throw away the jars or sanitize/keep?
I always reuse jars using this process:
How to Clean and Reuse Contaminated Jars (Mycology)
th-cam.com/video/FJmXVLYp954/w-d-xo.html
I'm on my first attempt, and at first, the mycelium was growing beautifully, but today, I noticed a spot of localised green mould near the top of the jar. What would be your advice? It hasn't spread yet, and it would be easy enough to scoop it out with a sterilised spoon and discard, but I'm worried that the whole lot might need to be thrown out.
Throw everything you used away. Otherwise you risk everything. Look up trichoderma
Good info. I have dealt with a bit of contamination over the years.
Can you still eat the mushrooms safely if they have bacterial blotch ?
I just got some golden teacher. And when I injected my bag, it looks very wet like this. Did I put to much? Do I have mold? Bacteria?? It's been 5 days.
Wet rot. Bacteria.
What about a 10:46 bacterial myc. set pushing out massive flushes of boomers? Because that what I had happen with the few Bac sets I have had.