NGBS2022 Talk 5: Dynamic motion during loading and activation of the yeast Replisome - Nynke Dekker

Dynamic motion during loading and activation of the yeast Replisome
Speaker: Nynke Dekker, Delft University of Technology, Netherlands
Chair: David Rueda, Imperial College, London/MRC-LMS, UK
Co-Chair: Geoff Holdgate, AstraZeneca, UK
Nynke Dekker describes her work on the DNA replication in yeast at the single molecule level. She used optical tweezers to trap DNA between the beads and then visualizes assembly of the fluorescently labelled replication proteins on the DNA. Alternatively, she captures pre-assembled DNA-protein complexes between the beads. This has enabled quantitation of both binding and diffusion of proteins on DNA. This has led to the first successful analysis of CMG motion on DNA and the effect of nucleotide state on this process.
Read more about Nynke Dekker’s work here:
www.tudelft.nl/en/faculty-of-...
You can find more about the Next Generation Biophysics Symposium 2022 here:
www3.mrc-lmb.cam.ac.uk/sites/...
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