[TALK 17] Mass Spectrometry in the LMB - Cat Franco

Mass Spectrometry in the LMB
Speaker: Cat Franco, MRC Laboratory of Molecular Biology, UK
2024 Introduction to Biophysical Techniques
The LMB Mass Spectrometry and Proteomics Facility houses a wide range of mass spectrometers enabling biological proteomics and structural proteomics. Biological proteomics involves the identification of proteins and their interactions and the comparative proteomics of cells and tissues. In structural proteomics purified proteins or their complexes are investigated by hydrogen-deuterium exchange or cross-linking mass spectrometry.
In this video, Cat begins with the question: what does a mass spectrometer measure and what are its mains components? She describes how samples are ionized, mass is analysed and detected by the mass spectrometer. She explains why mass spectrometers are often coupled to hplcs for sample preparation. She then follows with a detailed description of approaches used in the facility for biological mass spectrometry for proteomics including sample requirements, workflows and labelling strategies for quantification. In addition, she shows different data acquisitions methods for label-free quantification. Lastly, she shows approaches for structural proteomics via cross-linking or by hydrogen-deuterium exchange.
The Mass Spectrometry and Proteomics Facility is headed by Cat Franco.
Read more about the facility here: www2.mrc-lmb.c...
You can find more videos from the LMB 2024 Biophysics Techniques Lecture series here:
• Biophysical Techniques...
--
About the MRC Laboratory of Molecular Biology (LMB):
The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Our scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.
More links:
Official Site: www2.mrc-lmb.c...
Facebook: / mrc.lmb
X/Twitter: / mrc_lmb
Instagram: / mrc_lmb
LinkedIn: / mrc-laboratory-of-mole...
Click here to subscribe to MRC Laboratory of Molecular Biology on TH-cam: www.youtube.co...