Hello John :) Degradative enzymes in lysozymes are active in pH about 4,5-5 due to proton pump activity maintainting proper environment inside of it. In case on lysozyme damage ( for example during the proces of homogenization) this enzymes are not active because of too high pH of cell matrix (about 7,4). Please give me some feedback if You agree or not :) Best regards !!!! :)
These methodology videos are most useful. I watch all of them at 2x speed
All the knowledge in half the time and quickens up structural drawing
Thank you. Your video are very clearly and easily to understand.
This is great thank you!
Cara, muito bom esse vídeo! Eu estava com alguma dúvidas, mas você foi muito esclarecedor! Obrigado!
Who is the instructor? His English is so clear and intelligible, like the schematics/shapes he draws. I am jealous :)
very good explanation with basic theories
Te amo, haha, gracias por esta gran explicación :D
I needed this thank u
Very good video although i wish you would have explained the reason why the cells were attached in the wall on the tank in the first place
Hello John :) Degradative enzymes in lysozymes are active in pH about 4,5-5 due to proton pump activity maintainting proper environment inside of it. In case on lysozyme damage ( for example during the proces of homogenization) this enzymes are not active because of too high pH of cell matrix (about 7,4). Please give me some feedback if You agree or not :) Best regards !!!! :)
can we use this method to extract intacellular enzymes from cells and whats the name of the plastic flask u used?
when preparing the lysis buffer can one use HCl instead of NaCl
use acid instead of salt?
Can u plz tell how can we locate an unknown protein in a cell?? What technique we can use??
Hello ... is protein extraction and RNA isolation the same thing ?
dear can you contact me bcz i need something about the topic
@@katwil8431 no protein is RNA...
good video! but man the way u say culture flask!! i had to turn on captions on that... but no, you're fine dw
basicaly this method explain extraction intracellular protein, if u need extracellular maybe this jurnal vvhile help doi: 10.5539/jmbr.w7n1p50