And now that i think a little, unless FEI had changed the system in the last 3-4 years, you are not cooling the sample with the liquid nitrogen. you are putting liquid nitrogen to cool down the cold trap, which is a ring concentric with the sample, very near it, which is responsable for condensing all the contamination in the column. it improves the vacuum, and if the sample evaporates with the energy of the beam, condensates in there and not inside the column.
to cool the sample you usually need a special sample holder, with a liquid nitrogen vessel also. that is very common in biological samples observation, or in very reactive/heat sensitive samples.
inside tem, it requires ultra high vacuum. sweat and dirt on hand and fingers if in touch with the holder can lead to outgassing and it will take very long time to pump down.
Hello, I have recently joined as Technical Assistant at a university in India with a TEM facility. I need some of your suggestions for proper handling and its operation
to keep it clean ;-) with many people operating the instruments for thousands of hours, the knobs n all do get rather icky.. i always wear gloves to protect myself!
Can someone tell how to operate STEM in FEI TECNAI G2-20 twin?
6 ปีที่แล้ว
set eucentric height, go to STEM, set SS9, GL6 -> then use intensity focus to find ronchi, switch off diffraction, use objective focus to focus the beam and do pivot points, go to higer magn and switch on diffraction again, adjust the intesity focus to find ronchi again -> repeat until you have ronchi and focused spot (out of diffraction), then start search -> focus using z-heigh and fine with intesity focus, stigmate.... done... (ps do not forget to properly orient your sample before this)
And now that i think a little, unless FEI had changed the system in the last 3-4 years, you are not cooling the sample with the liquid nitrogen. you are putting liquid nitrogen to cool down the cold trap, which is a ring concentric with the sample, very near it, which is responsable for condensing all the contamination in the column. it improves the vacuum, and if the sample evaporates with the energy of the beam, condensates in there and not inside the column.
It's a very good tutorial. TEM looks much more automated than STM I am currently using.
the cold trap is not for the temperature control of the sample but for the contamination control.
Very nicely explained - I followed instructions and I got superb images from tecnai
@xudong53
I put a pdf of the script on our our wiki page for this instrumennt. You can also turn on captions with CC on the TH-cam.
to cool the sample you usually need a special sample holder, with a liquid nitrogen vessel also. that is very common in biological samples observation, or in very reactive/heat sensitive samples.
Thanks for a comprehensive introduction!
inside tem, it requires ultra high vacuum. sweat and dirt on hand and fingers if in touch with the holder can lead to outgassing and it will take very long time to pump down.
Hello, I have recently joined as Technical Assistant at a university in India with a TEM facility. I need some of your suggestions for proper handling and its operation
I did not realize that you could set the eucentricity by observing the diffraction pattern. Thanks!
10 kev사용하는거랑 차원이다르군!
thanks a lot... i find this video is very helpful... hope you had video to get electron diffraction pattern
Excelente vídeo. Obrigado.
thank you very much. it is really helpful
Thank you!
hi
If you have a steel, how can you prepare your sample for TEM examiniations?
I am nearly sure that you can find an electrolyte to use with a twin-jet electropolisher.
to keep it clean ;-) with many people operating the instruments for thousands of hours, the knobs n all do get rather icky.. i always wear gloves to protect myself!
Yeah, nice thing, You can send one to my University in Poland. We do not have such a new TEMs ;-)
Can someone tell how to operate STEM in FEI TECNAI G2-20 twin?
set eucentric height, go to STEM, set SS9, GL6 -> then use intensity focus to find ronchi, switch off diffraction, use objective focus to focus the beam and do pivot points, go to higer magn and switch on diffraction again, adjust the intesity focus to find ronchi again -> repeat until you have ronchi and focused spot (out of diffraction), then start search -> focus using z-heigh and fine with intesity focus, stigmate.... done... (ps do not forget to properly orient your sample before this)
nihao ma?
Shoutout to DR. WANG #LaTech
Taking him now.
yeap... but that is why i never touch the holder!
And its xp, not 98.
Wow
tejidos a croche bolsos
I know, it was a joke.
I Wouldn't have thought that anyone could send us TEM for free.
You've very dirty diaphragm. Charged dirt on 200 kV is not really good.
Nice microscope, to bad it runs on Windows! Doh!
hoi im tem
hOI!
not what I expected after looking up tem
Very nicely explained - I followed instructions and I got superb images from tecnai