Fixation by glutaraldehdye Wash with optimum tetraoxide Wash with pbs Dehydration with ethanol Propylene oxide Infiltrate with resin Embedding put resin inside a mold Oven for overnight 60 degrees Cut the plastic mold Sectioning by ultramicrotome
Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)
Fixation by glutaraldehdye
Wash with optimum tetraoxide
Wash with pbs
Dehydration with ethanol
Propylene oxide
Infiltrate with resin
Embedding put resin inside a mold
Oven for overnight 60 degrees
Cut the plastic mold
Sectioning by ultramicrotome
You deserve a raise!
😘😌o god 🙏.... Awesome yar... It's just amazing.
Excellent, very complicated. Thanks for sharing, enlightening us.
Thank you Ma'am for this clear explanation 😀
Very well explained
Thank you for your hardwork
Wow!! Amazed. Thank you
It's more complicated than I thought :D Thank you.
wow you saveddddd my life! best video
Very much intresting 👏👏👏
Very useful video of ultramicrotomy sample preparation and processing. Thank you very much.
Thank you mam🌺
Very Interesting and Amazing ☺👍
Thank you so much it was really helpful. Keep up the good work
Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)
thank you for sharing very clear and interesting explanation
Thanks a lot
very helpful thank you so much
hi i have a question, first at all dorry for my english!. to discard the osmiun u use a container onli for it? or u mix with the another discards? thx
“We are now sectioning our sample *machine beeps* oh god dang it” I felt that
can you tell me what was the specimen used in this prepration
@@ayeshairshad8681 no, they never mentioned what sample it is but my guess is tissue but looks more like plant
@@alejandratellez8848 ok thanks
can you tell me which resin to use for post embedding of plant samples? mention resin name please
how much time the sample should be incubated with Osmium Tetroxide
how long sample need to put osmium tetraoxide ? after stain with lead citrate then the sample can be taken for electron microscope ?
Why do we add uranyl acetate first and then lead citrate?
Thank you madam
How do you prepare red blood cells for SEM?
thanks
What is that drop in which you are finally dropping the grid?
Some of the steps the voice was cut. How much time needed to keep the sample with the stain? Same thing with the last step.
10:03 What is name of blue color container having water and knife in it?
60 degree temp incubation????
Can be applied for TiO2 sample preparation
do you really think this research reflects biological reality after all this processing?
Nice question
What was the resin name? please mention
Epoxy Resin....
Please what is the first staining reagent?? I heard urine acetate 🤔
Uranyl acetate