Blood Banking: Acid Elution Part 2
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- เผยแพร่เมื่อ 24 ต.ค. 2017
- How to perform an Acid Elution on a patient with a positive Direct Antiglobulin Test (DAT). The video also shows how to modify the procedure for specimens with an inadequate amount of packed RBCs.
Thanks for this video, I have blood bank clinicals tomorrow and this is something I'll be doing tomorrow. This was a great refresher because I didn't remember the different steps. Thanks again!
Glad to help! Hope your rotation is going well!
I just found your channel, i wish found early(my grade is not that great), this video makes it easier to understand the procedure since things have switched online and we have no Labs in person but have online work
Hope it helps bring up your grade! You can do it!!!!!
Same here!
@@macdaddyjill thank you for your support! Glad to help!
Thank you for this video
thankyou! it really helps me a lot ♥️
Thanks for your lectures here , i will follow you from Jordan , please keep on :)
Sahar Jamil
Thank you so much for your support! Best wishes to you!
Thanks for the video
thanx! very helpful🙏
Thank you for your guidelines, however I'm not sure I saw when you added the eluting solution (probably the camera didn't capture it or my eyes are playing with me). Would like to know how many mls or drops of the eluting solution you added. Thank you
Thanks for the class. Would like to know the difference between elution and adsorption and why do elution instead of adsorption
Thank you so much . It was so helpful because I am doing my project on elution. I subscribed you. However, one question how do you use the last wash for control?
Anusha
Thank you for your support!
The last wash is tested parallel to the eluate (as if it was the patient's plasma) using the antibody identification panel. The idea is that there should only be an antibody in the eluate and not the last wash.
The eluate should contain the antibody taken off the patient's RBCs. It is important to identify this antibody so as to ensure safe transfusions in the future.
The last wash should not contain any antibody. If there is a positive reaction to any of the reagent RBCs in the antibody identification panel it could mean a couple of things. The most likely cause would be the wash steps were not performed correctly and there are still some residual unbound antibodies floating around in the last wash. The other scenario is that the working wash solution liberated some of the antibodies from the patient's RBCs (which is not desirable). If this is the case, you might see a mirroring of a reaction pattern with the eluate or negative reactions from the eluate. In any case, you would need to perform the elution and antibody identification panel over again.
so 50ul of last wash and 25ul of eluate or the other way around? because for antibody Id I will have to use Panel A 50 ul of Rbcs and 25 ul of eluate. I would really appreciate your help.
Anusha
Using the tube method, you would have a set of tubes which would test the eluate against the reagent RBCs. At the same time, you would have another set of tubes which would be testing the last wash against the reagent RBCs. So, if you have a 10 cell panel you would have 10 tubes testing the eluate and 10 tubes testing the last wash (20 total).
You would treat the procedure the same as if you were doing an antibody identification panel with a patient specimen (1 drop of reagent cells and 2 drops of eluate or last wash in place of the plasma).
For how long do you do the wash? Min/rpm?
If only there was plenty sample for proficiency samples