Blood Banking: Antibody Identification Panel Interpretation Part 1
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- เผยแพร่เมื่อ 21 ส.ค. 2024
- How to interpret positive Antibody Screen and Identification Panel antigrams. This includes the importance of the autocontrol, IS, 37, AHG, and CC reactions.
You're amazing, keep it up. We need more educators like you!
Thank you so much! You made my day!
Thanks for your video, this helped me start to understand and get a clue of how to read and interpret antibody screens for my classes. Super helpful and easy to follow!
So glad it was helpful! Good luck in your course ; )
Omg the better explanation ! I want the copy for the sheet you have for you student! Thanks
Thanks so much,I now have a faint idea of what is antibody identification,and to solve my panel sheet questions.
Nnenna Ezeoke
Glad I could is help!
Thanks alot for the procedure..Good luck from Pakistan
i really love your video. You sincerely making this video so it is not boring to me. I am fully giving my attention to see your video rather in my class hahahha. You got a new subcriber. :D Please make more video to help MLTs student to be more interested in this course. ;D
Thank you for your support! Welcome to the MLLG Family!
Hi my lecturer gave a tutorial on this sheet with no explanation so your videos are really helping, I cannot see a thing though and really basing what you're referring to off what I saw in the previous video, your phone camera was better.
Q: if the AHG is positive with IS and 37 negative do I just assume that it's IgG and which column shows the control to identify whether it's an allo or auto antibody?
Thank you for your support and feedback!
A: Yes, IgG most likely will show up just in AHG because it is an enhanced reaction/visual of what happened in the 37 phase. The autocontrol line is where you are testing the patient's plasma against their own cells. This will show either an auto antibody or formation of a new allo antibody to an antigen on a recentently transfused RBC in the patient's blood. You would also need to look at the rest of the panel reactions in concert with the autocontrol to accurately interpret the results (auto vs allo antibody).
@@MedicalLabLadyGillwow this was quick, it's 4am in South Africa now. Thank you, I don't understand how to rule out, or why do you cross out the homozygous positives in the negative lines? Sorry if this is a stupid question
I'm still having issues understanding the dosage effect. I have read various definitions, but none of them really make whole lot of sense to me. Could you please explain the dosage effect or the meaning of "showing dosage."
Sure! It all comes down to how the antigen is inherited. Lets take the Rh grouping's Cc or Ee. Both the c's and e's are inherited together on the same gene. Each individual inherits one gene from each parent (1 from each gamete, sperm and egg). If the reagent cell donor gets a E gene from his/her mom and e from his/her dad, then that person is considered heterozygous. This would be represented as a + in both the E and e columns on the antigram. If the donor reagent cell is heterozygous, we generally see a weaker reaction with the patient's corresponding antibodies (Anti-E and/or Anti-e) in the AHG phase.
If the donor reagent cell is homozyogous for only E, there would be a + in the E column of the antigram and a 0 in the e column of the antigram. When this happens, there is a stronger reaction with the patient's corresponding Anti-E antibody (in the AHG phase) because only the E transmembrane protein is present on the donor's RBC membrane.
With that being said, think about having a limited (20) number of antigen proteins available for each gene. If 20 out of 20 proteins are E then one would expect the a larger reaction with Anti-E. If 10/20 proteins were E and 10/20 were e, then one would expect a smaller reaction with Anti-E.
Great video Gill! I was wondering, would it be possible to get a copy of the sheet you give to your students?
Thank you for the question. I am looking at releasing those sheets to educators soon. It would be part of a kit for an educational LIS.
really worthwhile. thanks!
thank u my teacher
Glad to help!
I love the view
Thank you!
It's so blurry though 😔
Would you happen to have a copy of your cheat sheet. I would like a copy. I am struggling with blood bank
thanks for this video highly appreciated, by the way, im a volunteer trainee at a component processing unit, I wonder if panocell is used for tube method, because I saw my staff also doing an antigram of Panel C, using gel card method, p.s. they're using OrthoInnova/ OrthoMax Vision machine, if ever in the future also make a video in terms of gel card and automation.
thanks again! looking forward for more educational and informative tutorials, you inspire us a lot. God Bless.😇
Pokemon Go
Thank you so much!!! Yes, these reagent cells would be used in the tube, gel card, and microwell methods. The patient plasma and reagent cell would be added to the upper portion of the gel card before the respective incubation and centrifugation steps.
I will do my best to meet your request. We do not have one of those analyzers on campus but I will see if I can persuade one of our clinical affiliates to let me video at their facility!
Thank you for your support!!! I am so thankful this channel is helpful 😇 God's blessings to you as well!!!
Pokemon Go
Also, thank you for the work you do!!! Component processing is soooo crucial to the industry and patient health!!!
Pokemon Go
Oops, I forgot to tell you that the reagent cells used for gel cards may be of a different percentage instead of the usual 3-5% required for tube testing. Check the package insert or manufacturer's guidelines of the method in use.
Medical Lab Lady Gill noted ma'am, looking forward for more helpful and educational videos. wishing you to have more viewers and subscribers because you deserve it, and kudos also to you your family :)
I would love to have that checklist in pdf
Can you share the cheat sheet you used pls ❤
Please give me your check list, I like it.
Do you need to use donor cells to do Ab identification for patient? Or just the company reagents (11panel)!?
Thank you and please if possible could you give us your paper sheet they are really good.
Working on putting a whole notes packet together 😉
could you post the cheat sheet?
Have you received. I would also like it
why did u skip over V* and C*?
How can we get a copy of your cheat sheet?
same question!
Big K
It's haisy l can't see anything
Wide angle lens on an Ab id panel video where the sheet of paper is huge and already difficult enough to understand? Seriously?
Yep. I guess so. Sorry it didn't work for you. Maybe better luck next time. There is a gel antibody ID video that may be better for you.
What does +s mean on an antigram panel?
They mean that an antigen (protein) is present on the donor cell's RBC membrane.