Super informative even after two years, I am considering buying a microscope and this video is the most informative I have found on the internet. Thank you ;)
Thank you. I haven't used a microscope ever until this week. your videos have been helpful in finding and using it. TH-cam likes recommending your videos and for good reasons! More visual examples when explaining something visual would be more pleasant to look at
I would have love to see a comparison images with cheap but still quite usable Aliexpress Vs quality eyepiece on a cheap microscope, then the same combination on the high end microscope.
Some eyepieces have much larger diameter lenses than others , whats the difference , pros, cons etc , i think i watched a vid of yours that addressed this specific subject but wasnt able to find it again . A link or title would be great , thanks
Would u be able to do a microscope video observing bacteriophage? That would be really cool, I’ve been looking around and can’t find any videos of them moving around. I can only find computer animations of them. Loving your videos btw!
I can not do this - because bacteriophages can not be seen with light microscopes. They are too small. You need electron microscopes to see them, but then you will not be able to see movement because specimens for electron microscopes must be completely dry. Movement would not be interesting to observe anyway, because bacteriophages (same like other viruses) do not move on their own but simply float with the fluid in which they are until they hit a cell to infect.
....When One is not using the scope, for any length of time, is there any recommendations as to how to cover (protect) the eye pieces, or to set the adjustments, to minimize any "wear and tear" from long term storage?.... Also: What about cleaning of the various lenses, etc?..... [Thank you, for another great video, on this kind of equipment!!!!]
Dust is a general problem for microscopy, not only for the eyepieces. The whole microscope should be covered (cloth, bag, box etc). Cleaning should be minimized. Objectives do not have to be cleaned (unliess oil immersion). Eyepieces can be cleaned to remove grease from the eyelashes with alcohol/ether (not with cleaning agents). I made another video on cleaning: th-cam.com/video/vbYY8qCDrhU/w-d-xo.html
I have a Zeize 47 30 11 - 9901 compound microscope , one of the eyepieces have what looks like a crack on the side of the lens going around , I am sure it is in the eyepiece because the crack moves with the eyepiece moved from one side to another , the eyepieces are KPL W10X18 with a eyeglasses symbol on the side of it , there is 3 objectives on it , Plam 10/0.22 , ph2 40/0.65 , planapo 100/1.3 Oel , the planapo 100/1.3 Oel have a large amount of dots in it , the revolving nose piece have a place for 5 objectives . first question , what do I need to upgrade ? second question , What is the meaning of eyeglasses image on a microscope eyepiece .
Could you do a video demonstration of the various types of eyepieces in terms of their glass element construction, and dismantling for cleaning the inner sides of the elements. I have found some dirt inside the barrel of my eyepiece between the two glass elements, but I am not confident about unscrewing the barrel in fear of getting the elements around the wrong way when reconstructing them, in the event one falls out before I get a chance of seeing which way they were put in. My fingers aren't the most nimblest of fingers, I admit, and these are small elements. It's delicate work. Thanks.
I'm almost sure each lens element of the eyepiece has a convex and concave side, could you please help me understand which direction each of them should point.
1:41 If we machine them and custom fit them with 3D printing, will it be possible to focus them to get a proper image? I still don't fully understand the magic in theses optics. Why 3 types? why not only one 10x with same distance and same focal for all 3 system?
Are the eye pieces manufacturer specific or can they be used interchangeably as long as they're the same type of microscope? Can I use eye pieces from a Nikon stereo microscope on an Olympus stereo microscope, etc?
Even from the same manufacturer, some eyepieces are designed to work only with specific objectives. This is very much a case where one wants to try eyepieces before buying.
Hello. I want to get a 20x eyepiece for my inspection microscope to use in electronics inspection (7x-45x body - eakins). I have option of WF20x/10 and WF20x/12 eyepieces. Any of this options is preferable over the other?
@microbehunter I live in the UK I'm looking the a wf eye piece with close eye relief which one can you recommend that I'm having issues with my current one as I can see my eyelashes and eyebrows it's so annoying I'm using a trinocular stereo microscope.thx
You need one with a long eye relief. Can not recommend a specific one, but look for those that have eyeglasses printed on them. They are for people who want to use the microscope with eyeglasses on, and they have a larger distance. Check diameter of eyepiece barrel, for stereo microscopes the diameter is about 30mm.
The total magnification can be calculated by multiplying the objective magnification by eyepiece magnification. For example, you can get 100x total magnification by using 10x eypiece and 10x objective (100=10*10) OR by using a 25x eyepiece and 4x objective (100=25*4). However the 25*4 combination will produce a more blurry image. The reason is that it is the objective that is responsible for collecting the image information. If the image information is not there in the first place, then you can not make this information by using a stronger eyepiece. The 10x objective can collect more image information (higher "numerical aperture") than the 4x, becasue the 10x is closer to the specimen. Example: if you use a wide angle camera lens (on a photo camera) then by zoomin in, you can also not see more detail. If you want to see more detail, you have to use a telephoto objective. The same idea here. If your camera has low megapixel, then you will also see a more blurry image when you zoom in (because the camera did not capture the image information in the first place).
It sound as though you are saying that: One must obtain a suitable, (compatible) "Photo Projection Occular", in order to adapt a camera/video/recorder device, to the scope's "trinocular" port....(?)...
A photo projection ocular is not absolutely necessary but improves the image quality as it compensates lens errors (aberrations) from the objective. It is designed also in such a way that the projected image is flat on the camera's sensor. Sometimes they are therefore called "compensating" oculars as they compensate the lens errors from the objective. But for them to work well, they have to be made by the same company (as they have to be adjusted to the objectives).
I have difficulties using a binocular optical microscope - I adjust the distance between the eyepieces, but still find it hard to achieve a single image. Am I supposed to smooth my eyes close into the eye-pieces, or keep them a few cm away?
check that the diopter adjustment is set appropriately (by turning the eyepieces). Some eyepieces have a long eye-relief (for people who wear glasses) and then you need a longer distance to the eyepiece. Check the image of both eyepieces separately, it should show the same part of the specimen (check border to border). If it does not show the same section, then this could mean that some prisms are dislocated (unlikely, but possible).
How can I improve the eye-relief of my AO 110 Microstar microscope? I find that it is almost impossible to maintain a full field of view because any movement of my head at all causes the field to shrink. It is extremely annoying and unnecessarily complicates the use of my microscope. Thx.
The eye relief is set. I do not think that you can change it. You can buy different eyepieces and try them out if they work better. Try changing the distance to the eyepeice. Maybe you have some with a large eye relief (used by people who wear glasses) but are looking through it too close.
@@Microbehunter Thx, I've thought of and tried all of the above. I guess I'll have to make do the best I can. I'm also trying to find out how to make my own dark field filter and where exactly to place it.
WK is German and stands for Weitfeld-Kompensation. It is a wide-field eyepiece that compensates (corrects) optical aberrations form the objectives. Designed to be used with the semiplan objectives. I found this information in the instruction manual here: www.science-info.net/docs/reichert/A4/Neovar.pdf
Can you combine any microscope objective and any microscope ocular? Are this parts completely interchangeable? E.g. you have a Zeiss microscope combined with a objective from Olympus or a Nikon microscope with a ocular from Zeiss? The ocular mount is normed to 23 mm so it will fit but will it work? The objectives are also standardized and you can change them. Will it work?
I know this comment is a year old, but the answer is yes and no. Certain brands often make their eyepieces to correct for aberration and other things resulting from their objectives. So yes, it will work. But you may not get the best results like you would with Nikon eyepiece and Nikon objectives, or Olympus eyepieces and Olympus objectives. Its best to stick to the same brand eyepieces as your objectives.
Do all trinocular microscopes hve such eye-pieces for the trinocular head to project the image into the camera lense? I have a couple of Bresser trinocular microscopes, one compound and one stereoscopic for viewing insects and such. Bought myself a T2-ring for my Nikon Dslr, and a adapter which connects to the T2 ring and has a metal tube that goes into the trinocular head. But there is no lenses in that and I have not had much success with photographing any subjects with my dslr. Or is such photography eyepieces reserved for higher end microscopes? I know Bresser has a eyepiece looking thing for the science grade microscopes, but not the ones I have it seems. But then I do not see why Bresser would make trinocular head microscopes with no photography dedicated eyepiece for those microscopes.
3:55 QUIZ QUESTION: What will be the difference: 5x Eye piece with 40x Objective 10x Eye piece with 20x Objective Both are giving a 200x total magnification, but what about the image quality? Colors, field of view, etc? If we want to use the setup with a Phone camera or just my bare eyes. What would be the best and why?
40x objective with a 5x eyepiece would be a higher resolution image. As he said in the video, the eyepiece doesn't magnify the image, it just enlarges whatever the objective projects to it.
@@intensecutn Ok, but how does this two image will compare for the field of view? I have a feeling that with the 5x, I will have half the field of view. Is this correct?
Hey microbe hunter look on your last video and look for my comment I have a whole bunch of questions I’m not bothered writing it and I can’t copy and paste it
![BOWKYLION - วิงวอน (ex-change) [Official MV]](http://i.ytimg.com/vi/cLdnZWDaO-w/mqdefault.jpg)
Super informative even after two years, I am considering buying a microscope and this video is the most informative I have found on the internet. Thank you ;)
Thank you. I haven't used a microscope ever until this week. your videos have been helpful in finding and using it.
TH-cam likes recommending your videos and for good reasons! More visual examples when explaining something visual would be more pleasant to look at
Great video! This and your video on objectives have been helpful. Now I want some 5x eye pieces.
I would have love to see a comparison images with cheap but still quite usable Aliexpress Vs quality eyepiece on a cheap microscope, then the same combination on the high end microscope.
Excellent helpfyl information. Thank you for compiling all of this.
Some eyepieces have much larger diameter lenses than others , whats the difference , pros, cons etc , i think i watched a vid of yours that addressed this specific subject but wasnt able to find it again . A link or title would be great , thanks
Would u be able to do a microscope video observing bacteriophage? That would be really cool, I’ve been looking around and can’t find any videos of them moving around. I can only find computer animations of them.
Loving your videos btw!
I can not do this - because bacteriophages can not be seen with light microscopes. They are too small. You need electron microscopes to see them, but then you will not be able to see movement because specimens for electron microscopes must be completely dry. Movement would not be interesting to observe anyway, because bacteriophages (same like other viruses) do not move on their own but simply float with the fluid in which they are until they hit a cell to infect.
Hi Oliver- would you mind clicking on closed caption so the deaf population can enjoy your video’s please.
very nice video, MH, thank you so much
Hallo Sir... can youre shae link to buy a Adaptor DSLR to Microscope yore use ...🙏
What do you call the last part with grid lines?is this can be attach on the microscope?
Thank you for the tutorial !
....When One is not using the scope, for any length of time, is there any recommendations as to how to cover (protect) the eye pieces, or to set the adjustments, to minimize any "wear and tear" from long term storage?.... Also: What about cleaning of the various lenses, etc?..... [Thank you, for another great video, on this kind of equipment!!!!]
Dust is a general problem for microscopy, not only for the eyepieces. The whole microscope should be covered (cloth, bag, box etc). Cleaning should be minimized. Objectives do not have to be cleaned (unliess oil immersion). Eyepieces can be cleaned to remove grease from the eyelashes with alcohol/ether (not with cleaning agents). I made another video on cleaning: th-cam.com/video/vbYY8qCDrhU/w-d-xo.html
Thank you for your videos! very helpful.
Can you plz tell us what's the difference between a plan objective and an achromatic objective? And which is better?
Very informative! thank you.
I have a Zeize 47 30 11 - 9901 compound microscope , one of the eyepieces have what looks like a crack on the side of the lens going around , I am sure it is in the eyepiece because the crack moves with the eyepiece moved from one side to another , the eyepieces are KPL W10X18 with a eyeglasses symbol on the side of it , there is 3 objectives on it , Plam 10/0.22 , ph2 40/0.65 , planapo 100/1.3 Oel ,
the planapo 100/1.3 Oel have a large amount of dots in it , the revolving nose piece have a place for 5 objectives .
first question ,
what do I need to upgrade ?
second question ,
What is the meaning of eyeglasses image on a microscope eyepiece .
Could you do a video demonstration of the various types of eyepieces in terms of their glass element construction, and dismantling for cleaning the inner sides of the elements. I have found some dirt inside the barrel of my eyepiece between the two glass elements, but I am not confident about unscrewing the barrel in fear of getting the elements around the wrong way when reconstructing them, in the event one falls out before I get a chance of seeing which way they were put in. My fingers aren't the most nimblest of fingers, I admit, and these are small elements. It's delicate work. Thanks.
Yes. Working on a video.
@@Microbehunter: Thank you.
I'm almost sure each lens element of the eyepiece has a convex and concave side, could you please help me understand which direction each of them should point.
1:41 If we machine them and custom fit them with 3D printing, will it be possible to focus them to get a proper image?
I still don't fully understand the magic in theses optics.
Why 3 types? why not only one 10x with same distance and same focal for all 3 system?
Outstanding. Thank you again. 👍
Are the eye pieces manufacturer specific or can they be used interchangeably as long as they're the same type of microscope? Can I use eye pieces from a Nikon stereo microscope on an Olympus stereo microscope, etc?
Even from the same manufacturer, some eyepieces are designed to work only with specific objectives.
This is very much a case where one wants to try eyepieces before buying.
Some eyepieces have an H before the magnification instead of WF ..what does it mean? thank u for the info
H is for a "High eyepoint". You can wear glasses while looking through the eyepiece (larger distance to the eyepiece is possible).
Hello. I want to get a 20x eyepiece for my inspection microscope to use in electronics inspection (7x-45x body - eakins). I have option of WF20x/10 and WF20x/12 eyepieces. Any of this options is preferable over the other?
Hello sir can you explain the difference between plan achromatic and semi plan achromatic and normal achromatic objectives
@microbehunter I live in the UK I'm looking the a wf eye piece with close eye relief which one can you recommend that I'm having issues with my current one as I can see my eyelashes and eyebrows it's so annoying I'm using a trinocular stereo microscope.thx
You need one with a long eye relief. Can not recommend a specific one, but look for those that have eyeglasses printed on them. They are for people who want to use the microscope with eyeglasses on, and they have a larger distance. Check diameter of eyepiece barrel, for stereo microscopes the diameter is about 30mm.
Most important Question NOT Answered: can you interchange eye piece? Can you just get a microscope eye piece from olympus and give it to Amscope?
he has already talked about that elsewhere and it has to do with 160 versus infinity
Thanks for the video. Do you know the difference between a khw and a hwf eyepiece?
good video !
Great info! "student proof..." Aahhhhaaaaahhhhaaaaaaaaahhaaa! 🤣
Could you please help me understand why magnifying the "image" through eyepieces makes it more blurry compared to object lens
The total magnification can be calculated by multiplying the objective magnification by eyepiece magnification. For example, you can get 100x total magnification by using 10x eypiece and 10x objective (100=10*10) OR by using a 25x eyepiece and 4x objective (100=25*4). However the 25*4 combination will produce a more blurry image. The reason is that it is the objective that is responsible for collecting the image information. If the image information is not there in the first place, then you can not make this information by using a stronger eyepiece. The 10x objective can collect more image information (higher "numerical aperture") than the 4x, becasue the 10x is closer to the specimen. Example: if you use a wide angle camera lens (on a photo camera) then by zoomin in, you can also not see more detail. If you want to see more detail, you have to use a telephoto objective. The same idea here. If your camera has low megapixel, then you will also see a more blurry image when you zoom in (because the camera did not capture the image information in the first place).
@@Microbehunter thank you!!
It sound as though you are saying that: One must obtain a suitable, (compatible) "Photo Projection Occular", in order to adapt a camera/video/recorder device, to the scope's "trinocular" port....(?)...
A photo projection ocular is not absolutely necessary but improves the image quality as it compensates lens errors (aberrations) from the objective. It is designed also in such a way that the projected image is flat on the camera's sensor. Sometimes they are therefore called "compensating" oculars as they compensate the lens errors from the objective. But for them to work well, they have to be made by the same company (as they have to be adjusted to the objectives).
I have difficulties using a binocular optical microscope - I adjust the distance between the eyepieces, but still find it hard to achieve a single image. Am I supposed to smooth my eyes close
into the eye-pieces, or keep them a few cm away?
check that the diopter adjustment is set appropriately (by turning the eyepieces). Some eyepieces have a long eye-relief (for people who wear glasses) and then you need a longer distance to the eyepiece. Check the image of both eyepieces separately, it should show the same part of the specimen (check border to border). If it does not show the same section, then this could mean that some prisms are dislocated (unlikely, but possible).
@@Microbehunter thanks i try to do that i dnt think the prob is the prisms cos i have 3 binocular microscope - nb: i wear glasses
How can I improve the eye-relief of my AO 110 Microstar microscope? I find that it is almost impossible to maintain a full field of view because any movement of my head at all causes the field to shrink. It is extremely annoying and unnecessarily complicates the use of my microscope. Thx.
The eye relief is set. I do not think that you can change it. You can buy different eyepieces and try them out if they work better. Try changing the distance to the eyepeice. Maybe you have some with a large eye relief (used by people who wear glasses) but are looking through it too close.
@@Microbehunter Thx, I've thought of and tried all of the above. I guess I'll have to make do the best I can. I'm also trying to find out how to make my own dark field filter and where exactly to place it.
Visit this other channel of mine, in the most recent video I show you how to make filters:
th-cam.com/channels/ZG2CV8y-tjzoy_zCsg2NJQ.html
My Reichert Neovar has WK 10x eyepieces, what does the K stands for? Thanks
WK is German and stands for Weitfeld-Kompensation. It is a wide-field eyepiece that compensates (corrects) optical aberrations form the objectives. Designed to be used with the semiplan objectives. I found this information in the instruction manual here: www.science-info.net/docs/reichert/A4/Neovar.pdf
Can you combine any microscope objective and any microscope ocular? Are this parts completely interchangeable? E.g. you have a Zeiss microscope combined with a objective from Olympus or a Nikon microscope with a ocular from Zeiss? The ocular mount is normed to 23 mm so it will fit but will it work? The objectives are also standardized and you can change them. Will it work?
I know this comment is a year old, but the answer is yes and no. Certain brands often make their eyepieces to correct for aberration and other things resulting from their objectives. So yes, it will work. But you may not get the best results like you would with Nikon eyepiece and Nikon objectives, or Olympus eyepieces and Olympus objectives.
Its best to stick to the same brand eyepieces as your objectives.
Do all trinocular microscopes hve such eye-pieces for the trinocular head to project the image into the camera lense? I have a couple of Bresser trinocular microscopes, one compound and one stereoscopic for viewing insects and such. Bought myself a T2-ring for my Nikon Dslr, and a adapter which connects to the T2 ring and has a metal tube that goes into the trinocular head. But there is no lenses in that and I have not had much success with photographing any subjects with my dslr. Or is such photography eyepieces reserved for higher end microscopes? I know Bresser has a eyepiece looking thing for the science grade microscopes, but not the ones I have it seems. But then I do not see why Bresser would make trinocular head microscopes with no photography dedicated eyepiece for those microscopes.
3:55 QUIZ QUESTION: What will be the difference:
5x Eye piece with 40x Objective
10x Eye piece with 20x Objective
Both are giving a 200x total magnification, but what about the image quality? Colors, field of view, etc?
If we want to use the setup with a Phone camera or just my bare eyes. What would be the best and why?
40x objective with a 5x eyepiece would be a higher resolution image. As he said in the video, the eyepiece doesn't magnify the image, it just enlarges whatever the objective projects to it.
@@intensecutn Ok, but how does this two image will compare for the field of view? I have a feeling that with the 5x, I will have half the field of view. Is this correct?
the best is Leitz periplan
By student proof you mean burglar proof? :)
Hey microbe hunter look on your last video and look for my comment I have a whole bunch of questions I’m not bothered writing it and I can’t copy and paste it
Here are the answers :-)
th-cam.com/video/7OM6DNg0s70/w-d-xo.html