Microscopy techniques basics | Microscopy lecture | magnification and resolution of a microscope
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- เผยแพร่เมื่อ 31 ก.ค. 2024
- Microscopy techniques basics | Microscopy lecture | magnification and resolution of a microscope - This microscopy lecture is going to explain the basics of light microscopy in details. light microscope working principle explained with this microscopy tutorial. Here we have discussed the following important topics -
1. Microscope parts and functions
2. Magnification of a microscope
3. Resolution of a microscope
4. Use of light microscope to visualize sample with staining.
So, if you a new to this field and want to know more about the basics of the light microscope working principle, stay tuned to this microscopy lecture tutorial.
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Thank you so much!! Your video is super helpful. I learned to understand this concept now for my Microbiology class.
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you said smaller the d, greater the resolution? but according to the diagram of two dots on board, as d decreases, resolution also decreases towards right side, and as d increases towards left side, resolution also increases.. please explain?
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If we use an oil immersion objective with blue light for sample illumination then wt will be the resolving power?
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I would like to ask what I should use as lambda in the formel for the limit resolution by epiflurescence. d=0,61.lambda/ NA
What is lambda by epiflurescence: excitatationwavelenght or emissionwavelengt?
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You just saved me one more time... I am attending a microscopy course where the professor is not always clear, especially for someone who doesn't a have a good enough physics background like me. After watching this, I have the basics to proceed and actually comprehensively follow the lectures. Thanks a lot, you really are a life saver for so many people around the world :)
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Sir, does the overall 'd' represent the distance between the sample slide and the objective?
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Various sources of illumination used in light microscopes ??
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In some questions there is given; value of 2 theta= 90° ..If we want calculate the value of resolving power..should I use the value theta or 2theta?
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Does magnification causes the entities to appear as one or are they already ?
do you have a lecture on binomial nomenclature?
Sir, how to calculate highest resolution expected from a bright field microscope?
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What should we take wavelength of white colour if it is being used as source ?
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Which objective is better at the same magnification : having aperture of 0.34 or 0.87 ?
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What was d? The distance between eye piece and object or the distance between objective lens and object?
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Read books and any piece of information on that topic available in such a way that you are able to explain the topic to a layman
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