You just saved me one more time... I am attending a microscopy course where the professor is not always clear, especially for someone who doesn't a have a good enough physics background like me. After watching this, I have the basics to proceed and actually comprehensively follow the lectures. Thanks a lot, you really are a life saver for so many people around the world :)
by watching your lectures and getting inspired by u sir I have now opted Bsc biotechnology instead of medical.....now all I am dependent is on ur lectures .... an ideal guru🙏🏻🙏🏻🙏🏻
Thank you so much for your video sir. I am messaging from Canada. Your videos provide me relief for a cell biology course im about to take. I have already failed it once and I am retaking it and just seeing and watching your videos provides me a really good idea and makes me more confident. I had this severe anxiety about succeeding in the course and with your videos I feel so much better. I subscribed and Im excited to see you hit 1million soon.
Got to your video from link mentioned in Kaunas University of Technollogy lecture slide. Proud to see Inndians recognised in Foreign universities as well. Good job bro.
you said smaller the d, greater the resolution? but according to the diagram of two dots on board, as d decreases, resolution also decreases towards right side, and as d increases towards left side, resolution also increases.. please explain?
Resolution is the ability to see two things apart As we move closer to the screen (d is decreasing) the two dots placed on the board will be clearly visible as two distinct dots And vice versa if we zoom out ( d is increasing) the two dots appears one
Great video sir, it's really helpful. Can u make a video on how to prepare ARS NET in Agriculture Biotechnology? I know you are the only one to sort out and make a video on it ...........please sir
Sir u mentioned that resolution is the ability to see two points seperately.. Then u mentioned d is inversely proportional to resolution..that means the closer the points are the better is the resolution(ability to see two points seperately) Is it that.. resolution is the ability to see two points seperately without missing it's finer details??
D means minimum distance b/w 2 points recognisable as 2 diff entities. If you can visualise 2 points clearly which are at 5 nm and other at 2.5 nm, which one would you prefer? Obviously the second one. So, d is inversely prop to resolution.
Thank you for the great video! I would like to ask what I should use as lambda in the formel for the limit resolution by epiflurescence. d=0,61.lambda/ NA What is lambda by epiflurescence: excitatationwavelenght or emissionwavelengt?
Can u tell me a idea how i will get a good technical knowledge ? For example i saw your video , i took notes also but is that information is enough for getting deapth knowledge ? Or i can read more related to that topics?
I have a doubt sir ,you say resolution is increases when we decrease 'd' ( distance between two points ) but sir if we decrease the distance between two points then points came near and nothing will be visible ,then how can we differentiate between two points Please reply me sir 🙏i am confused
Hello sir! I have a doubt...u said, higher the angle...higher will be the resolution and on the same time, higher the refractive index... higher will be the resolution. Right? But u also said, higher the refractive index, lesser will be the angle...eg. In case of oil...refractive index is 1.51 which is higher than air and water... it's angle will be less...then, it's resolution will be high! But how's it possible? It declines ur 1st statement that higher the angle, higher will be the resolution....! Kindly clear my doubt and thanks a lot for such an amazing explanation of this topic🙏🏻
The bestest teacher on TH-cam for lifescience 🤞. Thank you so much sir 😊💖
No doubt dude
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You just saved me one more time... I am attending a microscopy course where the professor is not always clear, especially for someone who doesn't a have a good enough physics background like me. After watching this, I have the basics to proceed and actually comprehensively follow the lectures. Thanks a lot, you really are a life saver for so many people around the world :)
I am glad to hear that you're getting benefit from my lectures
Yes! An indian guy always helps me with my Assignments.
by watching your lectures and getting inspired by u sir I have now opted Bsc biotechnology instead of medical.....now all I am dependent is on ur lectures .... an ideal guru🙏🏻🙏🏻🙏🏻
Thank you so much for appreciating my efforts
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He has just a white board and a marker, yet he helped me survive uni successfully :)
Thank you a lot sir!
That's all I need to make you fall in love with biology
Thank you so much for your video sir. I am messaging from Canada. Your videos provide me relief for a cell biology course im about to take. I have already failed it once and I am retaking it and just seeing and watching your videos provides me a really good idea and makes me more confident. I had this severe anxiety about succeeding in the course and with your videos I feel so much better. I subscribed and Im excited to see you hit 1million soon.
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Your lectures let me pass my experimental methods exam, thank you sir.
Your explanation is clear, keep the good work.
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@@shomusbiologyofficialsir meri exam hai but i am from Gujarati medium plz guide me
Is area se bhut sare question aate h CSIR net me..clear ho gya formula ab.thank u sir for making so simple..
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Well explained! I just ordered my first microscope. Thank you for expanding my knowledge ♥
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This was what I was not getting
Now it's 💯 % clear
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Sir, can't explain how much you are helping me during this corona time for self study..🙏
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Thank you so much!! Your video is super helpful. I learned to understand this concept now for my Microbiology class.
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I am so so proud of you ,you are always great , glad i found you . Thankyou so very much, god bless you ! 💐
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He is the best teacher... Thank you so much Sir for your helful videos 🤗🤗
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@@shomusbiologyofficial Thank you so much for your reply Sir🤗
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@@shomusbiologyofficial ok sir thnxx again
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Sir ap bht acha teach krte hai you are very nice teacher on TH-cam thnku so much🙏
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Ur lecture alwayz helpful ...I see ur total video sir ...Thanku u so much
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I am a msc student and your videos are more helpful for me
Excellent.thank u brother for giving such easy explanation.
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Sir BHT Sundar samjhaya h apne ...
Agar notes bhi mil jate to sone pe suhaga ho jata
You are really gifted by goddess saraswati , not only to learn things but to teach things too
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thank u sir,,i am learning from Bangladesh.keep it up sir.God bless u
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You are a good teacher. Thank you Sir
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Thank you so much sir.... your way of teaching is great 🙏😊
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Sir it's really helpful to understand the microscope thanks for guid us
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Got to your video from link mentioned in Kaunas University of Technollogy lecture slide. Proud to see Inndians recognised in Foreign universities as well. Good job bro.
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you said smaller the d, greater the resolution? but according to the diagram of two dots on board, as d decreases, resolution also decreases towards right side, and as d increases towards left side, resolution also increases.. please explain?
Resolution is the ability to see two things apart
As we move closer to the screen (d is decreasing) the two dots placed on the board will be clearly visible as two distinct dots
And vice versa if we zoom out ( d is increasing) the two dots appears one
Great video sir, it's really helpful. Can u make a video on how to prepare ARS NET in Agriculture Biotechnology? I know you are the only one to sort out and make a video on it ...........please sir
Okay
@@shomusbiologyofficial Thank u sir....
great explainer :)
Thank you
Sir u mentioned that resolution is the ability to see two points seperately..
Then u mentioned d is inversely proportional to resolution..that means the closer the points are the better is the resolution(ability to see two points seperately)
Is it that.. resolution is the ability to see two points seperately without missing it's finer details??
I'm having the same doubt if you got the answer then can you please explain
D means minimum distance b/w 2 points recognisable as 2 diff entities. If you can visualise 2 points clearly which are at 5 nm and other at 2.5 nm, which one would you prefer? Obviously the second one. So, d is inversely prop to resolution.
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Yup
Hello, I have question that what is answer of
Various sources of illumination used in light microscopes ??
You are Really good
Thank you
What was d? The distance between eye piece and object or the distance between objective lens and object?
you are the best thank you
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Tq so much Sir 🙏🙏
Fabulous man
Thank you
Thank you ❤️
Sir, does the overall 'd' represent the distance between the sample slide and the objective?
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This is a really great channel! I just subscribed. Could you also explain RICM ?
Good job sir
Thank you
Thanks sir aap bhaut acha pdhate h
Thank you so much for appreciating my efforts
Very very helpful
Thank you for the great video!
I would like to ask what I should use as lambda in the formel for the limit resolution by epiflurescence. d=0,61.lambda/ NA
What is lambda by epiflurescence: excitatationwavelenght or emissionwavelengt?
Superb
Thank you
Thank you sir
You're welcome
You are my hero
Thank you
In some questions there is given; value of 2 theta= 90° ..If we want calculate the value of resolving power..should I use the value theta or 2theta?
Sir, how to calculate highest resolution expected from a bright field microscope?
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Thanks a lot sir
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Sir ur grat sir plz answer me
If we use an oil immersion objective with blue light for sample illumination then wt will be the resolving power?
Can u tell me a idea how i will get a good technical knowledge ? For example i saw your video , i took notes also but is that information is enough for getting deapth knowledge ? Or i can read more related to that topics?
Read books and any piece of information on that topic available in such a way that you are able to explain the topic to a layman
I have a doubt sir ,you say resolution is increases when we decrease 'd' ( distance between two points ) but sir if we decrease the distance between two points then points came near and nothing will be visible ,then how can we differentiate between two points
Please reply me sir 🙏i am confused
Resolution is the capability to distinguish between two points
Hello sir!
I have a doubt...u said, higher the angle...higher will be the resolution and on the same time, higher the refractive index... higher will be the resolution. Right?
But u also said, higher the refractive index, lesser will be the angle...eg. In case of oil...refractive index is 1.51 which is higher than air and water... it's angle will be less...then, it's resolution will be high! But how's it possible?
It declines ur 1st statement that higher the angle, higher will be the resolution....!
Kindly clear my doubt and thanks a lot for such an amazing explanation of this topic🙏🏻
Sirr could you please tell me which book you referred for microscopic techniques
What should we take wavelength of white colour if it is being used as source ?
Which objective is better at the same magnification : having aperture of 0.34 or 0.87 ?