What is the PAM? - A CRISPR Whiteboard Lesson
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- เผยแพร่เมื่อ 2 ส.ค. 2024
- CRISPR-Cas9 searches and chops up invading viral DNA to defend bacterium against destruction. What prevents Cas9 from cutting the bacteria's own genome? The answer is the PAM sequence. Watch this whiteboard video to learn more about this important stretch of DNA. Video recorded and produced by Eugene Lo. Learn more in CRISPRpedia: innovativegenomics.org/crispr...
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Great...the best crispr video in youtube
indeed
Amazing video. Thank you for sharing.
The most excellent video I ever see. No one explains what is PAM and why it is important exactly until now.
I'm a simple housewife/grandma, but have been fascinated by CRISPR Cas9 since its first mentions in the news. Since then, I've striven to learn all I can, and have read Code Breaker (which was much more dramatic than I expected!) I'm truly happy to see it being used now to correct sickle cell and blindness, and have great hopes for the future. Thanks for this video, and for shedding a little more light into my understanding of the subject.
Couldn't find this explanation anywhere else, super clear and answered all my questions, great video really
Amazing video. Thats how a brilliant human explains science... Brilliant drawings.
Had to slow this down to 0.75 to take clear notes for myself lol Thanks for the clear explanation!
same , I kept pausing throughout and watched the video twice
Wow, that was extremely helpful! I don't normally comment on vids but I had to thank you for making such a fantastic video.
You have just answered my whole questions. Thank you. Amazing video.
your explanation is so clear but yet so thorough, salute from a non-bio phd trying to understand what my colleagues are doing...
Thank you! A lot of work went into this one. :)
This is an example of what well-explained means. Thanks a lot
A wonderful and thorough explanation of the Pam sequence. Thank you so much
Thank you for this OUTSTANDING video!
I have studied numerous sources to explain how PAM works and none came close to the clarity of this video....great work, thank you
Congrats on the video.
You explained what none of the all the most viewed and most liked videos didn't.
Needed this Video, because my professor rushed through the PAM part way too quickly.
Thankfully, you've explained the details and even the consequences for the "genome editing tool"
Thank you so much for creating this video! Finally I have a clear picture of how PAM works and why it is so useful!
Wow! Thank you for this video, it makes CRISPR Cas-9 so much easier to understand! Great vid
Great work, Eugene!
Such a good & clear explanation! The drawings really help the understanding. Thank you!
This video just goes to show that you don't need any fancy modeling animations. This white board explanation is better than anything else out there. 5-Stars.
Helping so much to understand the complex consent
Crystal clear explanation! Thank you!
This is super helpful
The pictures and explanation really helped me wrap my head around CRISPR
Thank you 😊 💓
Amazing! Incredible! Fantastic! Astounding! Mighty! Wonder! Super!
Thank you so so much for this video! It really helped me understand the detailed mechanisms of CRISPR Cas9 for my human developmental bio midterm presentation! Also going to be doing CRISPR undergraduate research this summer, so this is SUPER helpful! Cheers!
So happy we could help! Hope you enjoy your research experience. :)
Thank you! Just thank you!
This was so helpful! Thank you for creating this
Love this video! Great help! Thank you!
Just answered my whole question about CRISPR :") really helpful thank you soo much!!
This is a great video explaining the PAM, we only learned about the NGG PAM site. But now you explained that cas1 and cas2 look for different PAM site's it makes way more sense! Thank you, this will help me with my exam!
Glad you enjoyed the video and found it helpful! Most people are familiar with the NGG PAM because that is what SpyCas9 recognizes and SpyCas9 is the most common Cas9 protein used for genome editing. Other species of Cas9 have different PAM sequences. In our video at 3:10, we show that Cas9 works with the Cas1-Cas2 complex to find the PAM sequence and then Cas1-Cas2 removes the piece of viral DNA. In this instance Cas1-Cas2 is not recognizing the PAM sequence, but is working with Cas9. This is still and active area of research with many exciting questions to answer!
What course or institution is teaching and testing these concepts in class?
Amazing, thank you!
I watch the video a second time and is still incredible!
Excellent video...great job explaining PAM. Thank you!
Amazing explanation! Thank you so much
Really excellent video, thank you IGI
Awesome video! Super helpful. So glad I was able to find this.
this was the best video ive ever seen in my life
Excellent video
Thank you so much! I had a lecture about CRISPR and this PAM sequence was on many different slides but nowhere was explained it purpose nor what exactly actually this is :)
Ah amazing. Thank you so much. You are a live saver.
This help in my interview 👍 thanks a million!!
thanks for your input and great work! I will always remember PAM now :)
Thanks for a great video!
So helpful! The only video I could find to clearly understand the concepts for my exam!
Glad you enjoyed the video! Which school was teaching CRISPR in one of their courses? Also, we will be making more whiteboard videos and would love some suggestions about which CRISPR topics to explain.
RPI! We had a lecture on CRISPR/Cas9 and it's current uses in editing!
honestly, you deserve 10M like bro. Thank you
Awesome, thanks
the BEST explanation of CRISPR, hands down
This is amazing!
this video saved my life!! twice! hhhhh. It explained so well and the drawing makes it even more clear! Thank you so much for making this video!!!
thank you so much
Awesome video, thank you!
Incredible video, sooo helpful!
Incredible video! Helped me so much
fantastic video thank you
Brilliant!
AWESOME!!!!!!!
excellent, thank you
Thank you so much, this literally answered every question I possibly had.
So many of the very long videos entirely left aside either or both the evolutionary and recombinant highjacking significance of the PAM. This very short video gave necessary and sufficient description of both.
This was great. Tysm!
Thank you x 100!
Great video
I needed this video .. thank you so much
Thanks!
Outstanding!
Thank you so much. It helped
Seriously I love the way you question to every point and answering them nice 👌👌
Motived for my video 👌👌
love your introduction, it is so useful to me, much thanks!
It is really helpful, thanks a lot!
Great explanation, and you're great too.
thank you! amazing video, it helped me a lot
cannot thank you enough.
Muchas gracias por la explicación!, ahora la función de PAM me queda mas claro :)
Wow, I tried to understend that part in wikipedia. But it was impossible. Here everything seems so simple. Thanks
Brilliant~
thankyou! doing a SHE task on CRISPR and know i understand the topic
perfect !
Great video!!! It helped me a lot preparing for my exams!
We are curious to learn which school or course is teaching and testing these concepts?
My undergrad molecular genetics course at the University of Florida teaches a section of CRISPR-CAS9. Awesome video hope to see more
Awesome!
nice simple and straighforward
Excelent! i love it
Great job, ı havent seen any video about pam sequence before
incredibly explained
i love this video so much
amazing
I wish there were TH-cam videos like this when I was in college. 🙁
Thanks
Danke Bruder
Great video, i just wish that some references were attached in the description 😅 would make my essay writing so much easier hahaha
Thank yuuuuu
So it needs to have the GG after in order to attach
Please make more such content. You explained really well. I am doing a project on CRISPR so it'd be helpful.
Thank you! We're always interested in learning what topics people want to learn about, feel free to share your thoughts! And check out our website for lots of other CRISPR resources: innovativegenomics.org/
@@innovativegenomicsinstitute Sure I'll definitely check that out. :)
This is super helpful than you so much!!! :D
OMG bro i searched for hours to understand this but google just gives definition which explain nothing. In 5 minutes i Checked everything. Thx you bro i liked and subsribed
Glad you liked the video! We are always looking for new topics to explain, so please tell us if you would like to see another CRISPR concept animated.
@@innovativegenomicsinstitute i dont know what you could put new on the new vid this here had nearly every Thing:) i Need to Finish a presentation till wednesday this Video helped a lot to understand. I dont ''need" more Infos but a 2nd One would still be interesting!
Hello.. How can I get this lecture in the form of a PDF file
Hello.. How can I get this lecture in the form of a PDF or PowerPoint file ?
Great video but I wish you explained how the bacteria makes the TracrRNA that bind to the repeat RNA
I Love You.
You just saved me from a headache
Hello, I was brought to this video after clicking on a question and answer on Google and my question is related to the answer I was reading where it says "This Is Where The Pam Comes In The Pan Which Stands For Photo Spacer Adjacent Motif " So What I Would Like To Know Is What Is The Pan Cause Thats What The Pam Is And What Does Come In The Pan Mean Also? Thanks 😊
What if the target region never contains PAM, i.e. the NGG on the 3' side? Would that imply that CRISPR will fail at cutting out the sequence?
Hello. Can you make a video like this about cas12 or cas13? Thanks
You're in luck-we did! :) th-cam.com/video/e7SfWhfKf9s/w-d-xo.html