3. How to Read an ANA IFA Pattern - 3 Steps

In this educational video series we will discuss how to read ANA (antinuclear antibody) IFA patterns in HEp-2 substrate and how ANA IFA can aid in the diagnosis of autoimmune disease. This video tutorial will include three simple steps used in reading an ANA IFA.

[@juhaszsc]

Your videos are so clear to understand compared to the immunology class. Thank you for posting these.

[@romshasirkanungo3197]

Wonderful concise information.

[@imranafzal3973]

Good work.

[@junjunlupisan8569]

Hello! I just want to clarify: 200x is used to evaluate a titer if positive or negative, while 400x is for confirming patterns?

[@KaoruMina]

Gold!

[@ank6043]

Could you please answer my question?
Can ANA test (IFA, 1/80, IgG) show a response of the body to a metal implant (titanium screws on the jaw)?
Is an autoimmune/autoinflammatory response can be induced by metal adjuvants and may they cause a positive ANA test result?
Or, could we say a negative ANA test means there is no response of the body to the metal (titanium) implants?

[@bio-raddiagnostics9127]

LIVE WEBINAR November 16, 2022 - Autoantibody serology tests are not chemistry tests. Join Dr. Melissa Snyder from the Mayo Clinic as she discusses the heterogeneity of autoantibodies and approaches specifically for verifying autoantibody assays. Register: bit.ly/LRautoNov16

[@zipporaoosterling2468]

Very nice.
I would like to know the procedure for working with HEp-2 slides

[@bio-raddiagnostics9127]

The procedure is specific to the manufacturer, for Bio-Rad HEp-2 slides go to the following site www.bio-rad.com/en-us/category/hep-2-ifa?ID=38d7b598-d719-4887-b7c4-1373f1d68204

[@zipporaoosterling2468]

thank you

[@almasamir3717]

🔥🔥🔥👍👍👍

[@Lifeisgood-t3d]

Is there anyone that you could recommend I could consult about a very high anti-nuclear antibody’s test of 1290? I would be willing to do a phone/video consult if that is the only plausible way. I would so very much appreciate a response from you. Thanks you in advance.

[@shreestareddy5172]

Good evening ma'am/sir pls do reply recent i did ana test my report
ANA (HELP 2 CELLS )
Necloplasma -- Fine speckled
Nuclear membrane - negative
Nuclear dots -negative
Necleoil - negative
Cystoplasm - negative
Intensity -(+)
Impression - border line positive
Sir please explain my report

[@camdin8122]

Why shouldn't we read the center?

[@zipporaoosterling2468]

Why can't you read the edge?

[@bio-raddiagnostics9127]

The edge may have increased fluorescence intensity due to evaporation or decreased fluorescence intensity due to failure to apply reagents over the entire well