Glove boxes are no longer recommended as they have a tendency to create more air movement than a basic still air box. They are old tek that has been deprecated for several years now. Also, never flame anything inside a still air box.
The flame is what I was here to find out about. If you spray the entire area, aerosoloizing 70% isopropyl alcohol, how is that not risking combustion? Do you flame sterilize outside the box and then move the sterile tool inside?
@@benson733 Thank you. There's so many more steps and precautions to take with a SAB than with a laminar flow hood, but not everyone has $1000-$2000 to spend on a hobby 😒
Is this just for DIY stuff? That’s interesting I was under the impression real glove boxes (used in university labs) are basically completely sterile environments. I can definitely see how a DIY glove box might force more air movement tho, I’ve never used one
@@big_hat_logan I mean I use it for mycology and diy. I'm thinking the term glove box refers to a different type of box with gloves attached and that creates much more air flow than a standard still air box with only holes cut. You disinfect and then spray the box full of bleach water or even soapy water and let it sit for 10+ minutes. Then just use proper sterile techniques and you should have good low contamination rates. Laminar flow hoods are king though.
Do not flame sterilize inside the SAB!!! First, you're putting yourself in danger of igniting the isopropyl fumes, second the flame produces a lot of circulation inside the still air box, defeating the purpose of STILL AIR
So question, I’m over thinking it but the sterilizing the box thing before aren’t you letting in more contam opening it an putting everything inside? I had a bad lc from the jump so made me feel like I messed everything up an I went crazy with iso an sterilizing. Waiting for my new lc now an redoing this
Thanks for the video and a clip of what it looks like working in the box. Wondering - is it necessary to have a grill on the bottom so contaminants "fall through the cracks" below, and not on the same surface where your working items are? And for SABs, is 'bigger better'? My guess is that the larger the box, the less likely air movement will be an issue (due to hands/arms moving around the hole?) One last one - I see people complain about seeing through the plastic. But I don't see much online about people building SAB's out of glass (ie using old window glass, or even fish tanks). Any reason why someone wouldn't build a SAB out of glass (with silicone glue/sealant). Thanks!
Nice job, I like the idea of bleach instead of alcohol , you said 10% bleach but most consumer bleach is only 6% available chlorine ( usually hypochlorite ). Did you find a stronger bleach somewhere or did you mean you diluted household bleach at a 9:1 ratio with water ? I have hypochlorite available to me , its twice as strong as household bleach ( usually 12% but like bleach loses strength over time ) the weaker the solution the longer it takes to disinfect. I'm thinking a .3 or .4% chlorine solution would be plenty good. The smell is a bit worse than alcohol but worth it to eliminate the torch issue...cheers
Bleach only creates fumes for our purposes, its useless vs many spores. Even hospitals have stopped using it to sanitize. Also, ISO and bleach create chloroform. There is no need for it. Remember, its not a clean air box, its a still air box. There is no way you are gonna kill all the baddies in your box no matter how much bleach you spray. Thats why we use PHYSICS with a STILL AIR box. We wait until everything settles and try to leave it there as best we can. Its a simple, important theory that the maker of this video has left out.
Fwiw, I just picked up a 90 qt Room Essentials bin from Target for 13 bucks. It's maybe 10-15% larger than OP's. Flat lid, no handles/latches, and it sits flat upside down.
BLEACH AND ISO MAKE CHLOROFORM. I had to start there. Sorry mate but this is a poor instructional video that includes some dangerously bad advice. Who the hell lights a torch in an SAB sprayed down with ISO??? No offense mate, but you are gonna cause a nice boom or a hot ass flame inside your box. Ive done it decades ago and never have since. You can find all sorts of humorous and alarming stories of people doing this on the Shroomery. This is terrible advice to be giving to what are most likely noobs watching this video. Please, for everyone else's sake, edit this or put in a disclaimer about alcohol fumes in a small area and fire. LIGHTING A TORCH IN A BOX FILLED WITH ALCOHOL VAPOR IS A VERY BAD THING. YOU CAN START A SERIOUSLY BAD FIRE THIS WAY OR INJURE YOURSELF. Ok, so I let the video run on and you do clairify this, however you do show yourself lighting the torch inside the box. People might miss you saying that and see you doing it. Quite contradictive. Saying that.... ISO AND BLEACH MAKE CHLOROFORM. HOLY SHIT THAT IS SERIOUSLY BAD. Forget blowing up your SAB, this will wreck your lungs and cause some damage to your eyes and liver! Also, what are those... 4 inch holes? Dude, you are gonna be scraping your arms with those holes which will unleash aall sorts of microscopic shit along with your skin cells. If you are gonna use holes that small for gods sake use sleeves of some sort. Even saran wrap would be better than your bare skin. Another thing is that you are creating more air movement with small holes vs large ones. We are not making glove boxes anymore, those days are over (for most lol). Those are dogshit because of the piston effect. You are creating a piston effect by using such small holes. You really need to have at least 6 inch holes. I had 8 inch holes on my 200quart sterlite monster of a SAB (highly recommended @30 bucks. Target has one on sale now for $18!) Also working fast is not advised either. Slow, deliberate movements are necessary. The quicker you work the more shit you stir up. Take your time and dont rush anything. Im sorry if I sound like a dick, but please dont tell people to light up in their SABs or cook up some chloroform just to grow mushrooms.
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the way you make the holes is big brain !
certainly better than putting a bunch of microplastics in the air trying to shave away a good sized hole with a box cutter
Don't wipe the freshly flamed needle/scalpel with anything. Defeats the purpose..
for real! the soot is perfectly harmless.
Why flip the tub for when you use the gloves setup? Why not keep the lid on the bottom, same as without the gloves?
Glove boxes are no longer recommended as they have a tendency to create more air movement than a basic still air box. They are old tek that has been deprecated for several years now. Also, never flame anything inside a still air box.
The flame is what I was here to find out about. If you spray the entire area, aerosoloizing 70% isopropyl alcohol, how is that not risking combustion? Do you flame sterilize outside the box and then move the sterile tool inside?
@@ScottWConvid19Yes. You hold the tool outside and then bring it back in slowly while still hot....
@@benson733 Thank you. There's so many more steps and precautions to take with a SAB than with a laminar flow hood, but not everyone has $1000-$2000 to spend on a hobby 😒
Is this just for DIY stuff? That’s interesting I was under the impression real glove boxes (used in university labs) are basically completely sterile environments. I can definitely see how a DIY glove box might force more air movement tho, I’ve never used one
@@big_hat_logan I mean I use it for mycology and diy. I'm thinking the term glove box refers to a different type of box with gloves attached and that creates much more air flow than a standard still air box with only holes cut. You disinfect and then spray the box full of bleach water or even soapy water and let it sit for 10+ minutes. Then just use proper sterile techniques and you should have good low contamination rates. Laminar flow hoods are king though.
Reliable.
Do not flame sterilize inside the SAB!!! First, you're putting yourself in danger of igniting the isopropyl fumes, second the flame produces a lot of circulation inside the still air box, defeating the purpose of STILL AIR
He said this. He also was using a diluted bleach to sterilize, not iso.
Awesome. Non-locking totes are 6.98 at wally world right now 👍
So question, I’m over thinking it but the sterilizing the box thing before aren’t you letting in more contam opening it an putting everything inside? I had a bad lc from the jump so made me feel like I messed everything up an I went crazy with iso an sterilizing. Waiting for my new lc now an redoing this
Thanks for the video and a clip of what it looks like working in the box. Wondering - is it necessary to have a grill on the bottom so contaminants "fall through the cracks" below, and not on the same surface where your working items are? And for SABs, is 'bigger better'? My guess is that the larger the box, the less likely air movement will be an issue (due to hands/arms moving around the hole?) One last one - I see people complain about seeing through the plastic. But I don't see much online about people building SAB's out of glass (ie using old window glass, or even fish tanks). Any reason why someone wouldn't build a SAB out of glass (with silicone glue/sealant). Thanks!
Just more labour intensive. It's easier to spend the same ammount of time building a laminar flow hood.
A glass SAB would be nice. It will disinfect easier too.
Nice 😀👍
Nice job, I like the idea of bleach instead of alcohol , you said 10% bleach but most consumer bleach is only 6% available chlorine ( usually hypochlorite ). Did you find a stronger bleach somewhere or did you mean you diluted household bleach at a 9:1 ratio with water ? I have hypochlorite available to me , its twice as strong as household bleach ( usually 12% but like bleach loses strength over time ) the weaker the solution the longer it takes to disinfect. I'm thinking a .3 or .4% chlorine solution would be plenty good. The smell is a bit worse than alcohol but worth it to eliminate the torch issue...cheers
I believe he means 10% bleach to water mixure that you would make
@@sxrry.8206yeah he overthought a little
Bleach only creates fumes for our purposes, its useless vs many spores. Even hospitals have stopped using it to sanitize.
Also, ISO and bleach create chloroform. There is no need for it. Remember, its not a clean air box, its a still air box. There is no way you are gonna kill all the baddies in your box no matter how much bleach you spray. Thats why we use PHYSICS with a STILL AIR box. We wait until everything settles and try to leave it there as best we can.
Its a simple, important theory that the maker of this video has left out.
I have a question...can the paper sticker on the coffee-can catch fire?
Probably if it gets hot enough.
How many liters that???
I have the same question. Looks like 60 or so? Maybe a little bigger?
Fwiw, I just picked up a 90 qt Room Essentials bin from Target for 13 bucks. It's maybe 10-15% larger than OP's. Flat lid, no handles/latches, and it sits flat upside down.
Malegowski Polish surname. Thanks for the video.
I just wrecked mine as the plastic tore all down middle
Johnson Joseph Rodriguez Matthew Davis Kevin
BLEACH AND ISO MAKE CHLOROFORM. I had to start there.
Sorry mate but this is a poor instructional video that includes some dangerously bad advice.
Who the hell lights a torch in an SAB sprayed down with ISO??? No offense mate, but you are gonna cause a nice boom or a hot ass flame inside your box. Ive done it decades ago and never have since. You can find all sorts of humorous and alarming stories of people doing this on the Shroomery. This is terrible advice to be giving to what are most likely noobs watching this video. Please, for everyone else's sake, edit this or put in a disclaimer about alcohol fumes in a small area and fire.
LIGHTING A TORCH IN A BOX FILLED WITH ALCOHOL VAPOR IS A VERY BAD THING. YOU CAN START A SERIOUSLY BAD FIRE THIS WAY OR INJURE YOURSELF.
Ok, so I let the video run on and you do clairify this, however you do show yourself lighting the torch inside the box. People might miss you saying that and see you doing it. Quite contradictive. Saying that....
ISO AND BLEACH MAKE CHLOROFORM. HOLY SHIT THAT IS SERIOUSLY BAD. Forget blowing up your SAB, this will wreck your lungs and cause some damage to your eyes and liver!
Also, what are those... 4 inch holes? Dude, you are gonna be scraping your arms with those holes which will unleash aall sorts of microscopic shit along with your skin cells. If you are gonna use holes that small for gods sake use sleeves of some sort. Even saran wrap would be better than your bare skin.
Another thing is that you are creating more air movement with small holes vs large ones. We are not making glove boxes anymore, those days are over (for most lol). Those are dogshit because of the piston effect. You are creating a piston effect by using such small holes. You really need to have at least 6 inch holes. I had 8 inch holes on my 200quart sterlite monster of a SAB (highly recommended @30 bucks. Target has one on sale now for $18!)
Also working fast is not advised either. Slow, deliberate movements are necessary. The quicker you work the more shit you stir up. Take your time and dont rush anything.
Im sorry if I sound like a dick, but please dont tell people to light up in their SABs or cook up some chloroform just to grow mushrooms.
𝓟Ř𝔬𝓂𝔬𝐒ϻ
Nice try
I'll just add that bleach + rubbing alcohol = chloroform. Use one or the other in your workspace, never both.