Viscous oil is treated similarly to solids. Use “wet loading” if it is soluble in a solvent that is of equal polarity to your initial running condition. Use “dry loading” if it is soluble in a solvent that is more polar.
In our lab, we usually stick to fractions about 1/10th of the column volume. For example, if you use 25g of silica gel you should collect fractions of about 3 mL. This can be adjusted depending on your separation as well. Larger fractions can be taken for very good separations and smaller fractions for poor separations. This can help limit mixed fractions.
There is a good paper by W. Clark Still in JOC (Vol 43, No 14, 1978, pg 2923) which shows a table of typical fraction sizes with different size columns, volume of eluant, and sample loading size.
Thanks to all the team members for providing the wonderful content I hope you will make many more videos which helps many students, research scholars & others to get to know the basic understanding of such techniques & methods
Awesome video! super informative and very digestable. Also, I really dig the music. Any idea what the name of the track is? The song name isn't cited in the credits; only the composer is.
happy new year and Thank you so much for this video. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here? in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use? I mean is there ratio from silica gel to sample to use in column, please tell me
thank you. If I used wet packing of silica gel to column (slurry), here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
a piece of filter paper covering the porous glass filter plate, can facilitate glass washing, or even make it feasible and glass-lossless maybe... sometime f.e. the precipitate from the quenching of LAH RM, that sometimes unfiltered through cotton wool(for example), fortunately does not block filter paper, allowing filtering. my favorite chose with THF+LAH RM - to quench with a minimum of plain water(only H2O) - until whitening - adding not faster than 0.5 ml of the next portion of water every 30 minutes. Then white suspension diluted with an equal volume of DCM, shake and leave to settle for 30 minutes-to form compact(only 5ml from 1gm of LAH) crumbly solids, that can be filtered by gravity through cheesecloth... results flowing slow only with some rare waxy-nature products(but not with bad weather influence), that also accompanied with same difficulties in tradition LAH workup procedure, but even in that slow-workup synthesis - you will not need a three-liter Schott funnel instead of a one-liter cloth filter in pressurized-filtering-jar
If I have solid sample containing components, can I use any solvent to dissolve it? Or I have to use only the same mobile phase as solvent to dissolved sample? Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot in advance
So would this be considered 'Art Haus' or more 'Classic Cult"? Also, I didn't go to school for serious Chem, do people really think running Flash Chromatography more slowly gives better separation? *SMH* I guess they're confusing it with crystallization?
i am workin on column shromatography.. can you help me in guiding that is there any way of setting some manual punp system to my column that will convert it from standard column chromatography to flash chromatography...
Where in the world would i buy the powder for the silica gel or search on ebay or amazon to get it?!?! Please help me, and would any university carry it at their chemistry stock room? Thanks so much for the vid!
Conocí a colegas que purificaban en una bureta y corrían al Carls Jr por que están plenamente convencidos de que el sueño americano es posible para ellos.
Allway - chemistry rules at least the atomic world, I want president David Nutt (h t t p s : / / w w w.) th-cam.com/video/2Oc_GF65nbg/w-d-xo.html be protonic - be smart! :-))) even carbolic acids better than "neutral hydroxyl in society"
consuming a synthetic product is better than intake of unificated killing, you can even get rid of oxidation process - by reducing vinegar or acetaldehyde, but egregor's bad reputation defiles even something just marked by some individual sign of degradation
You use pressure to force the solvent mixture through the glass as opposed to relying on gravity alone to perform the run which would take a substantial amount of time in some cases
They deserve an oscar
💀💀💀
I was thinking the exact same thing! LMAO!
Such a cliff hanger too, did he get to pack her column at the end?
Really well done. Much better than any of the corporate TH-cams on the subject with a nice reference to the foundational publication on Flash.
5:25 Sick burn!
Jesus XD
Labs *this* clean only exist in movies =D
never partake crude
I liked how you cited a real scientific article and not just a blog's post, like every other tutorial on the internet :P, thanks
Viscous oil is treated similarly to solids. Use “wet loading” if it is soluble in a solvent that is of equal polarity to your initial running condition. Use “dry loading” if it is soluble in a solvent that is more polar.
In our lab, we usually stick to fractions about 1/10th of the column volume. For example, if you use 25g of silica gel you should collect fractions of about 3 mL. This can be adjusted depending on your separation as well. Larger fractions can be taken for very good separations and smaller fractions for poor separations. This can help limit mixed fractions.
There is a good paper by W. Clark Still in JOC (Vol 43, No 14, 1978, pg 2923) which shows a table of typical fraction sizes with different size columns, volume of eluant, and sample loading size.
This helped me review a lot to get started with my research thanks!
This video is incredible. Excellent work!
Wow this is a great video! I'll probably have to watch it a few times to keep up with everything however!
Best flash column tutorial on youtube that Ive seen
Awesome video! My mentor suggested I watch it and it was super helpful!!!!
Thanks to all the team members for providing the wonderful content I hope you will make many more videos which helps many students, research scholars & others to get to know the basic understanding of such techniques & methods
The best thing in this video is how much Silca is needed . You guys did a fabulous job👌
Great Video, Thank you so much💖💖💖💖 I will use the dry loading next time, unfortunately, I did not know about it before.
This was excellent,thanks guys.
Very Beautiful Demo and Explanation!
Thanks! This helps a lot :)
Awesome video! super informative and very digestable. Also, I really dig the music. Any idea what the name of the track is? The song name isn't cited in the credits; only the composer is.
Amazing video!
I would like to read the paper that it´s mention in the video, could you drop it here please.
happy new year and Thank you so much for this video. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here?
in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use?
I mean is there ratio from silica gel to sample to use in column, please tell me
Awesome video
Thank you people
thank you. If I used wet packing of silica gel to column (slurry),
here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
Great video. Cheers from UC Berk.
how can you load very viscous oils? -is it the same technique as drying?
-by the way great video
a piece of filter paper covering the porous glass filter plate, can facilitate glass washing, or even make it feasible and glass-lossless
maybe... sometime
f.e. the precipitate from the quenching of LAH RM, that sometimes unfiltered through cotton wool(for example), fortunately does not block filter paper, allowing filtering. my favorite chose with THF+LAH RM - to quench with a minimum of plain water(only H2O) - until whitening - adding not faster than 0.5 ml of the next portion of water every 30 minutes. Then white suspension diluted with an equal volume of DCM, shake and leave to settle for 30 minutes-to form compact(only 5ml from 1gm of LAH) crumbly solids, that can be filtered by gravity through cheesecloth...
results flowing slow only with some rare waxy-nature products(but not with bad weather influence), that also accompanied with same difficulties in tradition LAH workup procedure, but even in that slow-workup synthesis - you will not need a three-liter Schott funnel instead of a one-liter cloth filter in pressurized-filtering-jar
Educative yet entertaining...
What is the correct volume you should collect for each fraction? How does it depend on the amount of silica or crude?
If I have solid sample containing components,
can I use any solvent to dissolve it?
Or I have to use only the same mobile phase as solvent to dissolved sample?
Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot in advance
great video
Thanks from U of Montreal!
good job, thanks
Wow, another awesome video, Dr. Weizman. You know how to make learning Chemistry fun!
Nice video.
So cool👏🏽👏🏽👏🏽
So would this be considered 'Art Haus' or more 'Classic Cult"? Also, I didn't go to school for serious Chem, do people really think running Flash Chromatography more slowly gives better separation? *SMH* I guess they're confusing it with crystallization?
Nice Free advertisement fro Chemglass.
Great🌺👍
thanks
i am workin on column shromatography.. can you help me in guiding that is there any way of setting some manual punp system to my column that will convert it from standard column chromatography to flash chromatography...
I'm doing it tomorrow in the lab. Thanks from Freiburg, Germany :)
Where in the world would i buy the powder for the silica gel or search on ebay or amazon to get it?!?! Please help me, and would any university carry it at their chemistry stock room? Thanks so much for the vid!
sigmaaldrich.com
www.sigmaaldrich.com/catalog/search?term=silica+gel&interface=All&N=0&mode=match%20partialmax&lang=en®ion=US&focus=product
And later, Nathan gave Kristy a column of his own.
You are so disgusting!
With experiment both chemistry wahooooooo............
Oblivion dialogues in the Arcane University, Department of Alchemy
4:43 wait what, everytime i do that, the silica is stick into the flask
epic dialogue
why isn't she blinking the the first two minutes
All is well, except for TLC in a cup. There must be a Camera for TLC, saturated with eluent pairs.
Beautiful lady, beautiful chromatography, beautiful video!
Amé
Conocí a colegas que purificaban en una bureta y corrían al Carls Jr por que están plenamente convencidos de que el sueño americano es posible para ellos.
Kristy looks like she is having super hard time with her teeth.
5:23 Jesus.. Christie is sassy...
Wait, Dr. Haim Wiezman, a chemist... is he planning to become the President of Israel one day? :-D
Allway - chemistry rules at least the atomic world, I want president David Nutt
(h t t p s : / / w w w.) th-cam.com/video/2Oc_GF65nbg/w-d-xo.html
be protonic - be smart! :-)))
even carbolic acids better than "neutral hydroxyl in society"
consuming a synthetic product is better than intake of unificated killing, you can even get rid of oxidation process - by reducing vinegar or acetaldehyde, but egregor's bad reputation defiles even something just marked by some individual sign of degradation
4:54 no that's not a solution, it's a heterogeneous mixture.
it's not a ''pie pet'' but a pipette
Why is it called "flash" chromotography?
You use pressure to force the solvent mixture through the glass as opposed to relying on gravity alone to perform the run which would take a substantial amount of time in some cases
I would like that she takes care of my column.
No blood? This movie s not written and directed by Quentin Tarantino tho. Even though its an excelent one! lol
Amazing video!
I would like to read the paper that it´s mention in the video, could you drop it here please.
Maybe if you read it yourself, you wouldn't need a link. Ever think of that?